The miR-34a-LDHA axis regulates glucose metabolism and tumor growth in breast cancer
Lactate dehydrogenase A (LDHA) is involved in a variety of cancers. The purpose of this study was to investigate the expression, prognostic roles and function of LDHA in breast cancer. We found that LDHA was upregulated in both breast cancer cell lines and clinical specimens using quantitative real-...
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Veröffentlicht in: | Scientific reports 2016-02, Vol.6 (1), p.21735-21735, Article 21735 |
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Sprache: | eng |
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Zusammenfassung: | Lactate dehydrogenase A (LDHA) is involved in a variety of cancers. The purpose of this study was to investigate the expression, prognostic roles and function of LDHA in breast cancer. We found that LDHA was upregulated in both breast cancer cell lines and clinical specimens using quantitative real-time PCR (qRT-PCR). Immunohistochemistry (IHC) analysis of tissue microarrays (TMAs) showed that high LDHA expression was associated with cell proliferation, metastasis and poor patient overall survival (OS) and disease free survival (DFS). Furthermore, we found that LDHA promoted glycolysis and cell proliferation
in vitro
and
in vivo
. We also performed luciferase reporter assays and found that LDHA was a direct target of miR-34a. Repression of LDHA by miR-34a suppressed glycolysis and cell proliferation in breast cancer cells
in vitro
. Our findings provide clues regarding the role of miR-34a as a tumor suppressor in breast cancer through the inhibition of LDHA both
in vitro
and
in vivo
. Targeting LDHA through miR-34a could be a potential therapeutic strategy in breast cancer. |
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ISSN: | 2045-2322 2045-2322 |
DOI: | 10.1038/srep21735 |