Virus replicon particles expressing porcine reproductive and respiratory syndrome virus proteins elicit immune priming but do not confer protection from viremia in pigs
Porcine reproductive and respiratory syndrome virus (PRRSV) is the causative agent of one of the most devastating and economically significant viral disease of pigs worldwide. The vaccines currently available on the market elicit only limited protection. Recombinant vesicular stomatitis virus (VSV)...
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| Veröffentlicht in: | Veterinary research (Paris) 2016-02, Vol.47 (33), p.33-33, Article 33 |
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| creator | Eck, Melanie Durán, Margarita García Ricklin, Meret E Locher, Samira Sarraseca, Javier Rodríguez, María José McCullough, Kenneth C Summerfield, Artur Zimmer, Gert Ruggli, Nicolas |
| description | Porcine reproductive and respiratory syndrome virus (PRRSV) is the causative agent of one of the most devastating and economically significant viral disease of pigs worldwide. The vaccines currently available on the market elicit only limited protection. Recombinant vesicular stomatitis virus (VSV) replicon particles (VRP) have been used successfully to induce protection against influenza A virus (IAV) in chickens and bluetongue virus in sheep. In this study, VSV VRP expressing the PRRSV envelope proteins GP5, M, GP4, GP3, GP2 and the nucleocapsid protein N, individually or in combination, were generated and evaluated as a potential vector vaccine against PRRSV infection. High level expression of the recombinant PRRSV proteins was demonstrated in cell culture. However, none of the PRRSV antigens expressed from VRP, with the exception of the N protein, did induce any detectable antibody response in pigs before challenge infection with PRRSV. After challenge however, the antibody responses against GP5, GP4 and GP3 appeared in average 2 weeks earlier than in pigs vaccinated with the empty control VRP. No reduction of viremia was observed in the vaccinated group compared with the control group. When pigs were co-vaccinated with VRP expressing IAV antigens and VRP expressing PRRSV glycoproteins, only antibody responses to the IAV antigens were detectable. These data show that the VSV replicon vector can induce immune responses to heterologous proteins in pigs, but that the PRRSV envelope proteins expressed from VSV VRP are poorly immunogenic. Nevertheless, they prime the immune system for significantly earlier B-cell responses following PRRSV challenge infection. |
| doi_str_mv | 10.1186/s13567-016-0318-0 |
| format | Article |
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The vaccines currently available on the market elicit only limited protection. Recombinant vesicular stomatitis virus (VSV) replicon particles (VRP) have been used successfully to induce protection against influenza A virus (IAV) in chickens and bluetongue virus in sheep. In this study, VSV VRP expressing the PRRSV envelope proteins GP5, M, GP4, GP3, GP2 and the nucleocapsid protein N, individually or in combination, were generated and evaluated as a potential vector vaccine against PRRSV infection. High level expression of the recombinant PRRSV proteins was demonstrated in cell culture. However, none of the PRRSV antigens expressed from VRP, with the exception of the N protein, did induce any detectable antibody response in pigs before challenge infection with PRRSV. After challenge however, the antibody responses against GP5, GP4 and GP3 appeared in average 2 weeks earlier than in pigs vaccinated with the empty control VRP. No reduction of viremia was observed in the vaccinated group compared with the control group. When pigs were co-vaccinated with VRP expressing IAV antigens and VRP expressing PRRSV glycoproteins, only antibody responses to the IAV antigens were detectable. These data show that the VSV replicon vector can induce immune responses to heterologous proteins in pigs, but that the PRRSV envelope proteins expressed from VSV VRP are poorly immunogenic. Nevertheless, they prime the immune system for significantly earlier B-cell responses following PRRSV challenge infection.</description><identifier>ISSN: 1297-9716</identifier><identifier>ISSN: 0928-4249</identifier><identifier>EISSN: 1297-9716</identifier><identifier>DOI: 10.1186/s13567-016-0318-0</identifier><identifier>PMID: 26895704</identifier><language>eng</language><publisher>England: BioMed Central Ltd</publisher><subject>Animals ; antibodies ; antigens ; B-lymphocytes ; Bluetongue virus ; cell culture ; chickens ; Development and progression ; Genetic aspects ; glycoproteins ; Glycoproteins - metabolism ; Immune response ; Influenza A virus ; Life Sciences ; markets ; Medical research ; nucleocapsid proteins ; Porcine reproductive and respiratory syndrome ; Porcine Reproductive and Respiratory Syndrome - immunology ; Porcine Reproductive and Respiratory Syndrome - prevention & control ; Porcine Reproductive and Respiratory Syndrome - virology ; Porcine reproductive and respiratory syndrome virus ; Porcine respiratory and reproductive syndrome virus - immunology ; Properties ; protein synthesis ; replicon ; Replicon - immunology ; sheep ; Swine ; vaccines ; Vaccines, Synthetic - immunology ; Vesiculovirus ; Vesiculovirus - genetics ; Vesiculovirus - immunology ; Veterinary medicine ; Viral proteins ; Viral Proteins - metabolism ; Viral Vaccines - immunology ; viremia ; Viremia - immunology ; Viremia - prevention & control ; Viremia - veterinary ; Virion - immunology ; Virulence (Microbiology) ; viruses</subject><ispartof>Veterinary research (Paris), 2016-02, Vol.47 (33), p.33-33, Article 33</ispartof><rights>COPYRIGHT 2016 BioMed Central Ltd.</rights><rights>Copyright BioMed Central 2016</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><rights>Eck et al. 2016</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c561t-1f285e278bf234c1090397ed65a08a9352b2f9ba67945266090b06d2ede8f56c3</citedby><cites>FETCH-LOGICAL-c561t-1f285e278bf234c1090397ed65a08a9352b2f9ba67945266090b06d2ede8f56c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4761149/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4761149/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26895704$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://hal.science/hal-01341485$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Eck, Melanie</creatorcontrib><creatorcontrib>Durán, Margarita García</creatorcontrib><creatorcontrib>Ricklin, Meret E</creatorcontrib><creatorcontrib>Locher, Samira</creatorcontrib><creatorcontrib>Sarraseca, Javier</creatorcontrib><creatorcontrib>Rodríguez, María José</creatorcontrib><creatorcontrib>McCullough, Kenneth C</creatorcontrib><creatorcontrib>Summerfield, Artur</creatorcontrib><creatorcontrib>Zimmer, Gert</creatorcontrib><creatorcontrib>Ruggli, Nicolas</creatorcontrib><title>Virus replicon particles expressing porcine reproductive and respiratory syndrome virus proteins elicit immune priming but do not confer protection from viremia in pigs</title><title>Veterinary research (Paris)</title><addtitle>Vet Res</addtitle><description>Porcine reproductive and respiratory syndrome virus (PRRSV) is the causative agent of one of the most devastating and economically significant viral disease of pigs worldwide. The vaccines currently available on the market elicit only limited protection. Recombinant vesicular stomatitis virus (VSV) replicon particles (VRP) have been used successfully to induce protection against influenza A virus (IAV) in chickens and bluetongue virus in sheep. In this study, VSV VRP expressing the PRRSV envelope proteins GP5, M, GP4, GP3, GP2 and the nucleocapsid protein N, individually or in combination, were generated and evaluated as a potential vector vaccine against PRRSV infection. High level expression of the recombinant PRRSV proteins was demonstrated in cell culture. However, none of the PRRSV antigens expressed from VRP, with the exception of the N protein, did induce any detectable antibody response in pigs before challenge infection with PRRSV. After challenge however, the antibody responses against GP5, GP4 and GP3 appeared in average 2 weeks earlier than in pigs vaccinated with the empty control VRP. No reduction of viremia was observed in the vaccinated group compared with the control group. When pigs were co-vaccinated with VRP expressing IAV antigens and VRP expressing PRRSV glycoproteins, only antibody responses to the IAV antigens were detectable. These data show that the VSV replicon vector can induce immune responses to heterologous proteins in pigs, but that the PRRSV envelope proteins expressed from VSV VRP are poorly immunogenic. Nevertheless, they prime the immune system for significantly earlier B-cell responses following PRRSV challenge infection.</description><subject>Animals</subject><subject>antibodies</subject><subject>antigens</subject><subject>B-lymphocytes</subject><subject>Bluetongue virus</subject><subject>cell culture</subject><subject>chickens</subject><subject>Development and progression</subject><subject>Genetic aspects</subject><subject>glycoproteins</subject><subject>Glycoproteins - metabolism</subject><subject>Immune response</subject><subject>Influenza A virus</subject><subject>Life Sciences</subject><subject>markets</subject><subject>Medical research</subject><subject>nucleocapsid proteins</subject><subject>Porcine reproductive and respiratory syndrome</subject><subject>Porcine Reproductive and Respiratory Syndrome - immunology</subject><subject>Porcine Reproductive and Respiratory Syndrome - prevention & control</subject><subject>Porcine Reproductive and Respiratory Syndrome - virology</subject><subject>Porcine reproductive and respiratory syndrome virus</subject><subject>Porcine respiratory and reproductive syndrome virus - immunology</subject><subject>Properties</subject><subject>protein synthesis</subject><subject>replicon</subject><subject>Replicon - immunology</subject><subject>sheep</subject><subject>Swine</subject><subject>vaccines</subject><subject>Vaccines, Synthetic - immunology</subject><subject>Vesiculovirus</subject><subject>Vesiculovirus - genetics</subject><subject>Vesiculovirus - immunology</subject><subject>Veterinary medicine</subject><subject>Viral proteins</subject><subject>Viral Proteins - metabolism</subject><subject>Viral Vaccines - immunology</subject><subject>viremia</subject><subject>Viremia - immunology</subject><subject>Viremia - prevention & control</subject><subject>Viremia - veterinary</subject><subject>Virion - immunology</subject><subject>Virulence (Microbiology)</subject><subject>viruses</subject><issn>1297-9716</issn><issn>0928-4249</issn><issn>1297-9716</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNqFks1u1DAQgCMEomXhAbggS1zgkGIn_okvSKsKKNJKXICr5SSTravEDnay6r4Rj8mElNJWSChSYtnffDMZT5a9ZPSMsUq-S6wUUuWUyZyWrMrpo-yUFVrlWjH5-M76JHuW0hVFsBT8aXZSyEoLRflp9vO7i3MiEcbeNcGT0cbJNT0kAtdjhJSc35MxxMZ5WKgY2rmZ3AGI9S1upNFFO4V4JOno2xgGIIffRiQncB49KHYTccMwo2KMbliU9TyRNhAfJoJpO4hrAKqxiA49iwYGZ4nDotw-Pc-edLZP8OLmu8m-ffzw9fwi33359Pl8u8sbIdmUs66oBBSqqrui5A2jmpZaQSuFpZXVpSjqotO1lUpzUUiJ5zWVbQEtVJ2QTbnJ3q_eca4HaBvwU7S9Weq28WiCdeb-iXeXZh8OhivJGNcoeLsKLh-EXWx3ZtmjrOSMV-LAkH1zkyyGHzOkyQwuNdD31kOYk2EVlyWTlPP_o0oqWQhdSERfP0Cvwhw9dg0pxShOhRZ_qb3twTjfBfydZpGaLeZTgmMnkTr7B4VPi5eDNwedw_17AWwNaGJIKUJ32wNGzTK1Zp1abIM0y9Tia5O9utvz24g_Y1r-AgGE6mY</recordid><startdate>20160219</startdate><enddate>20160219</enddate><creator>Eck, Melanie</creator><creator>Durán, Margarita García</creator><creator>Ricklin, Meret E</creator><creator>Locher, Samira</creator><creator>Sarraseca, Javier</creator><creator>Rodríguez, María José</creator><creator>McCullough, Kenneth C</creator><creator>Summerfield, Artur</creator><creator>Zimmer, Gert</creator><creator>Ruggli, Nicolas</creator><general>BioMed Central Ltd</general><general>BioMed Central</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>88I</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>COVID</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>7X8</scope><scope>7S9</scope><scope>L.6</scope><scope>1XC</scope><scope>VOOES</scope><scope>5PM</scope></search><sort><creationdate>20160219</creationdate><title>Virus replicon particles expressing porcine reproductive and respiratory syndrome virus proteins elicit immune priming but do not confer protection from viremia in pigs</title><author>Eck, Melanie ; Durán, Margarita García ; Ricklin, Meret E ; Locher, Samira ; Sarraseca, Javier ; Rodríguez, María José ; McCullough, Kenneth C ; Summerfield, Artur ; Zimmer, Gert ; Ruggli, Nicolas</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c561t-1f285e278bf234c1090397ed65a08a9352b2f9ba67945266090b06d2ede8f56c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Animals</topic><topic>antibodies</topic><topic>antigens</topic><topic>B-lymphocytes</topic><topic>Bluetongue virus</topic><topic>cell culture</topic><topic>chickens</topic><topic>Development and progression</topic><topic>Genetic aspects</topic><topic>glycoproteins</topic><topic>Glycoproteins - metabolism</topic><topic>Immune response</topic><topic>Influenza A virus</topic><topic>Life Sciences</topic><topic>markets</topic><topic>Medical research</topic><topic>nucleocapsid proteins</topic><topic>Porcine reproductive and respiratory syndrome</topic><topic>Porcine Reproductive and Respiratory Syndrome - immunology</topic><topic>Porcine Reproductive and Respiratory Syndrome - prevention & control</topic><topic>Porcine Reproductive and Respiratory Syndrome - virology</topic><topic>Porcine reproductive and respiratory syndrome virus</topic><topic>Porcine respiratory and reproductive syndrome virus - immunology</topic><topic>Properties</topic><topic>protein synthesis</topic><topic>replicon</topic><topic>Replicon - immunology</topic><topic>sheep</topic><topic>Swine</topic><topic>vaccines</topic><topic>Vaccines, Synthetic - immunology</topic><topic>Vesiculovirus</topic><topic>Vesiculovirus - genetics</topic><topic>Vesiculovirus - immunology</topic><topic>Veterinary medicine</topic><topic>Viral proteins</topic><topic>Viral Proteins - 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Academic</collection><collection>AGRICOLA</collection><collection>AGRICOLA - Academic</collection><collection>Hyper Article en Ligne (HAL)</collection><collection>Hyper Article en Ligne (HAL) (Open Access)</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Veterinary research (Paris)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Eck, Melanie</au><au>Durán, Margarita García</au><au>Ricklin, Meret E</au><au>Locher, Samira</au><au>Sarraseca, Javier</au><au>Rodríguez, María José</au><au>McCullough, Kenneth C</au><au>Summerfield, Artur</au><au>Zimmer, Gert</au><au>Ruggli, Nicolas</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Virus replicon particles expressing porcine reproductive and respiratory syndrome virus proteins elicit immune priming but do not confer protection from viremia in pigs</atitle><jtitle>Veterinary research (Paris)</jtitle><addtitle>Vet Res</addtitle><date>2016-02-19</date><risdate>2016</risdate><volume>47</volume><issue>33</issue><spage>33</spage><epage>33</epage><pages>33-33</pages><artnum>33</artnum><issn>1297-9716</issn><issn>0928-4249</issn><eissn>1297-9716</eissn><abstract>Porcine reproductive and respiratory syndrome virus (PRRSV) is the causative agent of one of the most devastating and economically significant viral disease of pigs worldwide. The vaccines currently available on the market elicit only limited protection. Recombinant vesicular stomatitis virus (VSV) replicon particles (VRP) have been used successfully to induce protection against influenza A virus (IAV) in chickens and bluetongue virus in sheep. In this study, VSV VRP expressing the PRRSV envelope proteins GP5, M, GP4, GP3, GP2 and the nucleocapsid protein N, individually or in combination, were generated and evaluated as a potential vector vaccine against PRRSV infection. High level expression of the recombinant PRRSV proteins was demonstrated in cell culture. However, none of the PRRSV antigens expressed from VRP, with the exception of the N protein, did induce any detectable antibody response in pigs before challenge infection with PRRSV. After challenge however, the antibody responses against GP5, GP4 and GP3 appeared in average 2 weeks earlier than in pigs vaccinated with the empty control VRP. No reduction of viremia was observed in the vaccinated group compared with the control group. When pigs were co-vaccinated with VRP expressing IAV antigens and VRP expressing PRRSV glycoproteins, only antibody responses to the IAV antigens were detectable. These data show that the VSV replicon vector can induce immune responses to heterologous proteins in pigs, but that the PRRSV envelope proteins expressed from VSV VRP are poorly immunogenic. Nevertheless, they prime the immune system for significantly earlier B-cell responses following PRRSV challenge infection.</abstract><cop>England</cop><pub>BioMed Central Ltd</pub><pmid>26895704</pmid><doi>10.1186/s13567-016-0318-0</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record> |
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| issn | 1297-9716 0928-4249 1297-9716 |
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| source | MEDLINE; DOAJ Directory of Open Access Journals; SpringerLink Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; PubMed Central Open Access; Springer Nature OA Free Journals |
| subjects | Animals antibodies antigens B-lymphocytes Bluetongue virus cell culture chickens Development and progression Genetic aspects glycoproteins Glycoproteins - metabolism Immune response Influenza A virus Life Sciences markets Medical research nucleocapsid proteins Porcine reproductive and respiratory syndrome Porcine Reproductive and Respiratory Syndrome - immunology Porcine Reproductive and Respiratory Syndrome - prevention & control Porcine Reproductive and Respiratory Syndrome - virology Porcine reproductive and respiratory syndrome virus Porcine respiratory and reproductive syndrome virus - immunology Properties protein synthesis replicon Replicon - immunology sheep Swine vaccines Vaccines, Synthetic - immunology Vesiculovirus Vesiculovirus - genetics Vesiculovirus - immunology Veterinary medicine Viral proteins Viral Proteins - metabolism Viral Vaccines - immunology viremia Viremia - immunology Viremia - prevention & control Viremia - veterinary Virion - immunology Virulence (Microbiology) viruses |
| title | Virus replicon particles expressing porcine reproductive and respiratory syndrome virus proteins elicit immune priming but do not confer protection from viremia in pigs |
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