Changes in the immunohistochemical localization of the glycine receptor in the superior olivary complex of adult circling mice
Circling mice is a mutant model of spontaneous deafness exhibiting degenerated spiral ganglion cells in the cochlea and loss of organ of Corti. The balance between glycinergic inhibition and glutamatergic excitation in the lateral superior olive (LSO) is essential for the detection of inte-raural le...
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| Veröffentlicht in: | Molecular medicine reports 2015-12, Vol.12 (6), p.7927-7932 |
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| creator | YOO, YOUNG-BOK MASKEY, DHIRAJ KIM, MYEUNG JU |
| description | Circling mice is a mutant model of spontaneous deafness exhibiting degenerated spiral ganglion cells in the cochlea and loss of organ of Corti. The balance between glycinergic inhibition and glutamatergic excitation in the lateral superior olive (LSO) is essential for the detection of inte-raural level differences. Long term weakening of glycinergic synaptic inhibition in the LSO may lead to the downregulation of synaptic release of glycine in dorsal cochlear nucleus and downregulation of postsynaptic glycine receptor (GlyR) activity in the LSO, which may contribute to hearing loss. The present study utilized an immunohistochemical method to assess changes in GlyR immunoreactivity (IR) and the cell number in the superior olivary complex (SOC) of heterozygote (+/cir) and homozygote (cir/cir) circling mice. A significant decrease in the IR was observed in all nuclei of the SOC of homozygous mice. Loss of GlyR immunoreactive cells and a decrement in cell size was also observed in the homozygotes. A decrease in the GlyR IR in the neurons and neuropils, cell number and size of the cir/cir, may lead to profound changes in inhibitory transmission and the functional properties in the SOC nuclei. Therefore, the functional loss of inhibitory neurotransmitters in the brainstem may result in deafness of adult cir/cir mice. |
| doi_str_mv | 10.3892/mmr.2015.4431 |
| format | Article |
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The balance between glycinergic inhibition and glutamatergic excitation in the lateral superior olive (LSO) is essential for the detection of inte-raural level differences. Long term weakening of glycinergic synaptic inhibition in the LSO may lead to the downregulation of synaptic release of glycine in dorsal cochlear nucleus and downregulation of postsynaptic glycine receptor (GlyR) activity in the LSO, which may contribute to hearing loss. The present study utilized an immunohistochemical method to assess changes in GlyR immunoreactivity (IR) and the cell number in the superior olivary complex (SOC) of heterozygote (+/cir) and homozygote (cir/cir) circling mice. A significant decrease in the IR was observed in all nuclei of the SOC of homozygous mice. Loss of GlyR immunoreactive cells and a decrement in cell size was also observed in the homozygotes. A decrease in the GlyR IR in the neurons and neuropils, cell number and size of the cir/cir, may lead to profound changes in inhibitory transmission and the functional properties in the SOC nuclei. Therefore, the functional loss of inhibitory neurotransmitters in the brainstem may result in deafness of adult cir/cir mice.</description><identifier>ISSN: 1791-2997</identifier><identifier>EISSN: 1791-3004</identifier><identifier>DOI: 10.3892/mmr.2015.4431</identifier><identifier>PMID: 26498980</identifier><language>eng</language><publisher>Greece: D.A. Spandidos</publisher><subject>Animals ; Brain ; Brain stem ; Cell number ; Cell Size ; circling mice ; Cochlea ; Cochlear nuclei ; Deafness ; Deafness - genetics ; Deafness - metabolism ; Deafness - pathology ; Deoxyribonucleic acid ; DNA ; Ganglion cells ; Glutamatergic transmission ; glycine receptor ; Glycine receptors ; Hearing loss ; Homozygotes ; Immunohistochemistry ; Immunoreactivity ; Laboratories ; lateral superior olive ; Localization ; Male ; medial nucleus of trapezoid body ; Mice ; Mice, Inbred C57BL ; Neurons ; Neurotransmitters ; Olivary complex ; Organ of Corti ; Physiological aspects ; Receptors, Glycine - analysis ; Receptors, Glycine - genetics ; Receptors, Glycine - metabolism ; Spiral ganglion ; Studies ; Superior Olivary Complex</subject><ispartof>Molecular medicine reports, 2015-12, Vol.12 (6), p.7927-7932</ispartof><rights>Copyright: © Yoo et al.</rights><rights>COPYRIGHT 2015 Spandidos Publications</rights><rights>Copyright Spandidos Publications UK Ltd. 2015</rights><rights>Copyright: © Yoo et al. 2015</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c514t-118466e0c783416b4a5a4d890b2d5a3f2e79e9754e6491fd54aa4b00f2eb6f4e3</citedby><cites>FETCH-LOGICAL-c514t-118466e0c783416b4a5a4d890b2d5a3f2e79e9754e6491fd54aa4b00f2eb6f4e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,780,784,885,5570,27923,27924</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26498980$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>YOO, YOUNG-BOK</creatorcontrib><creatorcontrib>MASKEY, DHIRAJ</creatorcontrib><creatorcontrib>KIM, MYEUNG JU</creatorcontrib><title>Changes in the immunohistochemical localization of the glycine receptor in the superior olivary complex of adult circling mice</title><title>Molecular medicine reports</title><addtitle>Mol Med Rep</addtitle><description>Circling mice is a mutant model of spontaneous deafness exhibiting degenerated spiral ganglion cells in the cochlea and loss of organ of Corti. The balance between glycinergic inhibition and glutamatergic excitation in the lateral superior olive (LSO) is essential for the detection of inte-raural level differences. Long term weakening of glycinergic synaptic inhibition in the LSO may lead to the downregulation of synaptic release of glycine in dorsal cochlear nucleus and downregulation of postsynaptic glycine receptor (GlyR) activity in the LSO, which may contribute to hearing loss. The present study utilized an immunohistochemical method to assess changes in GlyR immunoreactivity (IR) and the cell number in the superior olivary complex (SOC) of heterozygote (+/cir) and homozygote (cir/cir) circling mice. A significant decrease in the IR was observed in all nuclei of the SOC of homozygous mice. Loss of GlyR immunoreactive cells and a decrement in cell size was also observed in the homozygotes. A decrease in the GlyR IR in the neurons and neuropils, cell number and size of the cir/cir, may lead to profound changes in inhibitory transmission and the functional properties in the SOC nuclei. Therefore, the functional loss of inhibitory neurotransmitters in the brainstem may result in deafness of adult cir/cir mice.</description><subject>Animals</subject><subject>Brain</subject><subject>Brain stem</subject><subject>Cell number</subject><subject>Cell Size</subject><subject>circling mice</subject><subject>Cochlea</subject><subject>Cochlear nuclei</subject><subject>Deafness</subject><subject>Deafness - genetics</subject><subject>Deafness - metabolism</subject><subject>Deafness - pathology</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>Ganglion cells</subject><subject>Glutamatergic transmission</subject><subject>glycine receptor</subject><subject>Glycine receptors</subject><subject>Hearing loss</subject><subject>Homozygotes</subject><subject>Immunohistochemistry</subject><subject>Immunoreactivity</subject><subject>Laboratories</subject><subject>lateral superior olive</subject><subject>Localization</subject><subject>Male</subject><subject>medial nucleus of trapezoid body</subject><subject>Mice</subject><subject>Mice, Inbred C57BL</subject><subject>Neurons</subject><subject>Neurotransmitters</subject><subject>Olivary complex</subject><subject>Organ of Corti</subject><subject>Physiological aspects</subject><subject>Receptors, Glycine - analysis</subject><subject>Receptors, Glycine - genetics</subject><subject>Receptors, Glycine - metabolism</subject><subject>Spiral ganglion</subject><subject>Studies</subject><subject>Superior Olivary Complex</subject><issn>1791-2997</issn><issn>1791-3004</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNptksuL1TAUxosozji6dCsFF7rpNc-m2QjDxRcMuNF1SNPTNkOa1KQdHBf-7abeO1dHJJDX-Z0vOclXFM8x2tFGkjfTFHcEYb5jjOIHxTkWElcUIfbwOCdSirPiSUrXCNWccPm4OCM1k41s0Hnxcz9qP0AqrS-XEUo7TasPo01LMCNM1mhXupB7-0MvNvgy9L-5wd0a66GMYGBeQrzLT-sM0eZ1cPZGx9vShGl28H3L093qltLYaJz1Q5nF4WnxqNcuwbPjeFF8ff_uy_5jdfX5w6f95VVlOGZLhXHD6hqQEQ1luG6Z5pp1jUQt6bimPQEhQQrOIBeG-44zrVmLUA60dc-AXhRvD7rz2k7QGfBL1E7N0U75kipoq-5HvB3VEG4UE7whEmeB10eBGL6tkBY12WTAOe0hrElhQTmlVCCU0Zf_oNdhjT6Xp7CkhAlaY_aHGrQDZX0f8rlmE1WXjEqMZM1Fpnb_oXLrtr8JHnqb9-8lVIcEE0NKEfpTjRipzTAqG0ZthlGbYTL_4u-HOdF3DsnAqwOQZu0724V0YrJShUmF6kpIIugvBizKeQ</recordid><startdate>20151201</startdate><enddate>20151201</enddate><creator>YOO, YOUNG-BOK</creator><creator>MASKEY, DHIRAJ</creator><creator>KIM, MYEUNG JU</creator><general>D.A. 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MASKEY, DHIRAJ ; KIM, MYEUNG JU</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c514t-118466e0c783416b4a5a4d890b2d5a3f2e79e9754e6491fd54aa4b00f2eb6f4e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Animals</topic><topic>Brain</topic><topic>Brain stem</topic><topic>Cell number</topic><topic>Cell Size</topic><topic>circling mice</topic><topic>Cochlea</topic><topic>Cochlear nuclei</topic><topic>Deafness</topic><topic>Deafness - genetics</topic><topic>Deafness - metabolism</topic><topic>Deafness - pathology</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>Ganglion cells</topic><topic>Glutamatergic transmission</topic><topic>glycine receptor</topic><topic>Glycine receptors</topic><topic>Hearing loss</topic><topic>Homozygotes</topic><topic>Immunohistochemistry</topic><topic>Immunoreactivity</topic><topic>Laboratories</topic><topic>lateral superior olive</topic><topic>Localization</topic><topic>Male</topic><topic>medial nucleus of trapezoid body</topic><topic>Mice</topic><topic>Mice, Inbred C57BL</topic><topic>Neurons</topic><topic>Neurotransmitters</topic><topic>Olivary complex</topic><topic>Organ of Corti</topic><topic>Physiological aspects</topic><topic>Receptors, Glycine - analysis</topic><topic>Receptors, Glycine - genetics</topic><topic>Receptors, Glycine - metabolism</topic><topic>Spiral ganglion</topic><topic>Studies</topic><topic>Superior Olivary Complex</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>YOO, YOUNG-BOK</creatorcontrib><creatorcontrib>MASKEY, DHIRAJ</creatorcontrib><creatorcontrib>KIM, MYEUNG JU</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>ProQuest Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>British Nursing Database</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection (Proquest) (PQ_SDU_P3)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Biological Sciences</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biological Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Molecular medicine reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>YOO, YOUNG-BOK</au><au>MASKEY, DHIRAJ</au><au>KIM, MYEUNG JU</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Changes in the immunohistochemical localization of the glycine receptor in the superior olivary complex of adult circling mice</atitle><jtitle>Molecular medicine reports</jtitle><addtitle>Mol Med Rep</addtitle><date>2015-12-01</date><risdate>2015</risdate><volume>12</volume><issue>6</issue><spage>7927</spage><epage>7932</epage><pages>7927-7932</pages><issn>1791-2997</issn><eissn>1791-3004</eissn><abstract>Circling mice is a mutant model of spontaneous deafness exhibiting degenerated spiral ganglion cells in the cochlea and loss of organ of Corti. The balance between glycinergic inhibition and glutamatergic excitation in the lateral superior olive (LSO) is essential for the detection of inte-raural level differences. Long term weakening of glycinergic synaptic inhibition in the LSO may lead to the downregulation of synaptic release of glycine in dorsal cochlear nucleus and downregulation of postsynaptic glycine receptor (GlyR) activity in the LSO, which may contribute to hearing loss. The present study utilized an immunohistochemical method to assess changes in GlyR immunoreactivity (IR) and the cell number in the superior olivary complex (SOC) of heterozygote (+/cir) and homozygote (cir/cir) circling mice. A significant decrease in the IR was observed in all nuclei of the SOC of homozygous mice. Loss of GlyR immunoreactive cells and a decrement in cell size was also observed in the homozygotes. A decrease in the GlyR IR in the neurons and neuropils, cell number and size of the cir/cir, may lead to profound changes in inhibitory transmission and the functional properties in the SOC nuclei. Therefore, the functional loss of inhibitory neurotransmitters in the brainstem may result in deafness of adult cir/cir mice.</abstract><cop>Greece</cop><pub>D.A. Spandidos</pub><pmid>26498980</pmid><doi>10.3892/mmr.2015.4431</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Molecular medicine reports, 2015-12, Vol.12 (6), p.7927-7932 |
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| source | Spandidos Publications Journals; MEDLINE; Alma/SFX Local Collection; EZB Electronic Journals Library |
| subjects | Animals Brain Brain stem Cell number Cell Size circling mice Cochlea Cochlear nuclei Deafness Deafness - genetics Deafness - metabolism Deafness - pathology Deoxyribonucleic acid DNA Ganglion cells Glutamatergic transmission glycine receptor Glycine receptors Hearing loss Homozygotes Immunohistochemistry Immunoreactivity Laboratories lateral superior olive Localization Male medial nucleus of trapezoid body Mice Mice, Inbred C57BL Neurons Neurotransmitters Olivary complex Organ of Corti Physiological aspects Receptors, Glycine - analysis Receptors, Glycine - genetics Receptors, Glycine - metabolism Spiral ganglion Studies Superior Olivary Complex |
| title | Changes in the immunohistochemical localization of the glycine receptor in the superior olivary complex of adult circling mice |
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