Crystallization of membrane proteins by vapor diffusion

X-ray crystallography remains the most robust method to determine protein structure at the atomic level. However, the bottlenecks of protein expression and purification often discourage further study. In this chapter, we address the most common problems encountered at these stages. Based on our expe...

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Veröffentlicht in:	Methods in enzymology 2015, Vol.557, p.363-392
Hauptverfasser:	Delmar, Jared A, Bolla, Jani Reddy, Su, Chih-Chia, Yu, Edward W
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Bacteria - chemistry Bacteria - genetics Bacterial Proteins - chemistry Bacterial Proteins - genetics Bacterial Proteins - isolation & purification Crystallography, X-Ray - methods Detergents - chemistry Diffusion Gene Expression Humans Membrane Proteins - chemistry Membrane Proteins - genetics Membrane Proteins - isolation & purification Models, Molecular Volatilization
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