Beneficial effect of pentoxifylline into the testis of rats in an experimental model of unilateral hindlimb ischemia/reperfusion injury
The objective of the present study was to investigate the role of pentoxifylline (PTX) on remote testicular injury caused by unilateral hind limb ischemia/reperfusion of rats. Twenty healthy male Wistar rats were allocated randomly into two groups: ischemia/reperfusion (IR group) and ischemia/reperf...
Gespeichert in:
| Veröffentlicht in: | International Brazilian Journal of Urology 2015-06, Vol.41 (3), p.576-583 |
|---|---|
| Hauptverfasser: | , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 583 |
|---|---|
| container_issue | 3 |
| container_start_page | 576 |
| container_title | International Brazilian Journal of Urology |
| container_volume | 41 |
| creator | Takhtfooladi, Mohammad Ashrafzadeh Moayer, Fariborz Takhtfooladi, Hamed Ashrafzadeh |
| description | The objective of the present study was to investigate the role of pentoxifylline (PTX) on remote testicular injury caused by unilateral hind limb ischemia/reperfusion of rats.
Twenty healthy male Wistar rats were allocated randomly into two groups: ischemia/reperfusion (IR group) and ischemia/reperfusion + pentoxifylline (IR+PTX group). Ischemia was induced by placement of a rubber tourniquet at the greater trochanter for 2h. Rats in IR+PTX group received PTX (40 mg/kg IP) before the reperfusion period. At 24h after reperfusion, testes were removed and levels of superoxide dismutase (SOD), malondialdehyde (MDA), catalase (CAT) and myeloperoxidase (MPO) activity were determined in testicular tissues. Three rats of each group were used for wet/ dry weight ratio measurement. Testicular tissues were also examined histopathologically under light microscopy.
Activities of SOD and CAT in testicular tissues were decreased by ischemia/ reperfusion (P |
| doi_str_mv | 10.1590/S1677-5538.IBJU.2014.0263 |
| format | Article |
| fullrecord | <record><control><sourceid>pubmed_doaj_</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_4752154</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><doaj_id>oai_doaj_org_article_d11251ec41214b2aa1d484f74d0f4e24</doaj_id><sourcerecordid>26200554</sourcerecordid><originalsourceid>FETCH-LOGICAL-c528t-1d7fcb3cc864f61196c6d35c2726edf4cfebce4a8e94083d34d5584b843fc93b3</originalsourceid><addsrcrecordid>eNpVkd1uGyEQhVHUKknTvkJFH8AOv_tzU6mJmsRVpF60uUYsDDHW7mIBruIn6GsH7DZKroAzcz4YDkJfKFlS2ZPLX7Rp24WUvFuurn48LBmhYklYw0_Q-aHUUNq_e7U_Qx9S2hDCetLSU3TGGkaIlOIc_b2CGZw3Xo8YnAOTcXB4C3MOT97tx9HPgH054bwGnCFln2pH1DkVHesZw9MWop-KpTCmYGGsDbvZjzpDLNraz3b004B9MmuYvL6MUCxul3yYC2Szi_uP6L3TY4JP_9YL9HDz_ff13eL-5-3q-tv9wkjW5QW1rTMDN6ZrhKuTNaaxXBrWsgasE8bBYEDoDnpBOm65sFJ2YugEd6bnA79AqyPXBr1R2_JuHfcqaK8OQoiPSsfszQjKUsokBSMoo2JgWlMrOuFaYYkTwERhfT2ytrthAmvKD5Rx30DfVma_Vo_hjxKtZFRWQH8EmBhSiuBevJSomrQ6JK1q0qomrWrSqiZdvJ9fX_7i_B8tfwYxfKpR</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>Beneficial effect of pentoxifylline into the testis of rats in an experimental model of unilateral hindlimb ischemia/reperfusion injury</title><source>PubMed (Medline)</source><source>MEDLINE</source><source>DOAJ Directory of Open Access Journals</source><source>EZB-FREE-00999 freely available EZB journals</source><source>PubMed Central Open Access</source><creator>Takhtfooladi, Mohammad Ashrafzadeh ; Moayer, Fariborz ; Takhtfooladi, Hamed Ashrafzadeh</creator><creatorcontrib>Takhtfooladi, Mohammad Ashrafzadeh ; Moayer, Fariborz ; Takhtfooladi, Hamed Ashrafzadeh</creatorcontrib><description>The objective of the present study was to investigate the role of pentoxifylline (PTX) on remote testicular injury caused by unilateral hind limb ischemia/reperfusion of rats.
Twenty healthy male Wistar rats were allocated randomly into two groups: ischemia/reperfusion (IR group) and ischemia/reperfusion + pentoxifylline (IR+PTX group). Ischemia was induced by placement of a rubber tourniquet at the greater trochanter for 2h. Rats in IR+PTX group received PTX (40 mg/kg IP) before the reperfusion period. At 24h after reperfusion, testes were removed and levels of superoxide dismutase (SOD), malondialdehyde (MDA), catalase (CAT) and myeloperoxidase (MPO) activity were determined in testicular tissues. Three rats of each group were used for wet/ dry weight ratio measurement. Testicular tissues were also examined histopathologically under light microscopy.
Activities of SOD and CAT in testicular tissues were decreased by ischemia/ reperfusion (P<0.05). Significantly increased MDA levels in testicular tissues were decreased by PTX treatment (P<0.05). MPO activity in testicular tissues in the IR group was significantly higher than in the IR+PTX group (P<0.05). The wet/dry weight ratio of testicular tissues in the IR group was significantly higher than in the IR+PTX group (P<0.05). Histopathologically, there was a statistically significant difference between two groups (P<0.05).
According to histological and biochemical findings, we conclude that PTX has preventive effects in the testicular injury induced by hind limb ischemia/reperfusion.</description><identifier>ISSN: 1677-6119</identifier><identifier>ISSN: 1677-5538</identifier><identifier>EISSN: 1677-6119</identifier><identifier>DOI: 10.1590/S1677-5538.IBJU.2014.0263</identifier><identifier>PMID: 26200554</identifier><language>eng</language><publisher>Brazil: Sociedade Brasileira de Urologia</publisher><subject>Animals ; Catalase - analysis ; Disease Models, Animal ; Free Radical Scavengers - pharmacology ; Hindlimb ; Hindlimb - blood supply ; Ischemia ; Ischemia - complications ; Ischemia - prevention & control ; Male ; Malondialdehyde - analysis ; Original ; Oxidative Stress ; Pentoxifylline ; Pentoxifylline - pharmacology ; Peroxidase - analysis ; Random Allocation ; Rats, Wistar ; Reperfusion ; Reperfusion Injury - complications ; Reperfusion Injury - prevention & control ; Reproducibility of Results ; Superoxide Dismutase - analysis ; Testicular Diseases ; Testis - chemistry ; Testis - drug effects ; Testis - metabolism ; Testis - pathology ; Time Factors ; Treatment Outcome</subject><ispartof>International Brazilian Journal of Urology, 2015-06, Vol.41 (3), p.576-583</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c528t-1d7fcb3cc864f61196c6d35c2726edf4cfebce4a8e94083d34d5584b843fc93b3</citedby><cites>FETCH-LOGICAL-c528t-1d7fcb3cc864f61196c6d35c2726edf4cfebce4a8e94083d34d5584b843fc93b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4752154/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4752154/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26200554$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Takhtfooladi, Mohammad Ashrafzadeh</creatorcontrib><creatorcontrib>Moayer, Fariborz</creatorcontrib><creatorcontrib>Takhtfooladi, Hamed Ashrafzadeh</creatorcontrib><title>Beneficial effect of pentoxifylline into the testis of rats in an experimental model of unilateral hindlimb ischemia/reperfusion injury</title><title>International Brazilian Journal of Urology</title><addtitle>Int Braz J Urol</addtitle><description>The objective of the present study was to investigate the role of pentoxifylline (PTX) on remote testicular injury caused by unilateral hind limb ischemia/reperfusion of rats.
Twenty healthy male Wistar rats were allocated randomly into two groups: ischemia/reperfusion (IR group) and ischemia/reperfusion + pentoxifylline (IR+PTX group). Ischemia was induced by placement of a rubber tourniquet at the greater trochanter for 2h. Rats in IR+PTX group received PTX (40 mg/kg IP) before the reperfusion period. At 24h after reperfusion, testes were removed and levels of superoxide dismutase (SOD), malondialdehyde (MDA), catalase (CAT) and myeloperoxidase (MPO) activity were determined in testicular tissues. Three rats of each group were used for wet/ dry weight ratio measurement. Testicular tissues were also examined histopathologically under light microscopy.
Activities of SOD and CAT in testicular tissues were decreased by ischemia/ reperfusion (P<0.05). Significantly increased MDA levels in testicular tissues were decreased by PTX treatment (P<0.05). MPO activity in testicular tissues in the IR group was significantly higher than in the IR+PTX group (P<0.05). The wet/dry weight ratio of testicular tissues in the IR group was significantly higher than in the IR+PTX group (P<0.05). Histopathologically, there was a statistically significant difference between two groups (P<0.05).
According to histological and biochemical findings, we conclude that PTX has preventive effects in the testicular injury induced by hind limb ischemia/reperfusion.</description><subject>Animals</subject><subject>Catalase - analysis</subject><subject>Disease Models, Animal</subject><subject>Free Radical Scavengers - pharmacology</subject><subject>Hindlimb</subject><subject>Hindlimb - blood supply</subject><subject>Ischemia</subject><subject>Ischemia - complications</subject><subject>Ischemia - prevention & control</subject><subject>Male</subject><subject>Malondialdehyde - analysis</subject><subject>Original</subject><subject>Oxidative Stress</subject><subject>Pentoxifylline</subject><subject>Pentoxifylline - pharmacology</subject><subject>Peroxidase - analysis</subject><subject>Random Allocation</subject><subject>Rats, Wistar</subject><subject>Reperfusion</subject><subject>Reperfusion Injury - complications</subject><subject>Reperfusion Injury - prevention & control</subject><subject>Reproducibility of Results</subject><subject>Superoxide Dismutase - analysis</subject><subject>Testicular Diseases</subject><subject>Testis - chemistry</subject><subject>Testis - drug effects</subject><subject>Testis - metabolism</subject><subject>Testis - pathology</subject><subject>Time Factors</subject><subject>Treatment Outcome</subject><issn>1677-6119</issn><issn>1677-5538</issn><issn>1677-6119</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>DOA</sourceid><recordid>eNpVkd1uGyEQhVHUKknTvkJFH8AOv_tzU6mJmsRVpF60uUYsDDHW7mIBruIn6GsH7DZKroAzcz4YDkJfKFlS2ZPLX7Rp24WUvFuurn48LBmhYklYw0_Q-aHUUNq_e7U_Qx9S2hDCetLSU3TGGkaIlOIc_b2CGZw3Xo8YnAOTcXB4C3MOT97tx9HPgH054bwGnCFln2pH1DkVHesZw9MWop-KpTCmYGGsDbvZjzpDLNraz3b004B9MmuYvL6MUCxul3yYC2Szi_uP6L3TY4JP_9YL9HDz_ff13eL-5-3q-tv9wkjW5QW1rTMDN6ZrhKuTNaaxXBrWsgasE8bBYEDoDnpBOm65sFJ2YugEd6bnA79AqyPXBr1R2_JuHfcqaK8OQoiPSsfszQjKUsokBSMoo2JgWlMrOuFaYYkTwERhfT2ytrthAmvKD5Rx30DfVma_Vo_hjxKtZFRWQH8EmBhSiuBevJSomrQ6JK1q0qomrWrSqiZdvJ9fX_7i_B8tfwYxfKpR</recordid><startdate>20150601</startdate><enddate>20150601</enddate><creator>Takhtfooladi, Mohammad Ashrafzadeh</creator><creator>Moayer, Fariborz</creator><creator>Takhtfooladi, Hamed Ashrafzadeh</creator><general>Sociedade Brasileira de Urologia</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20150601</creationdate><title>Beneficial effect of pentoxifylline into the testis of rats in an experimental model of unilateral hindlimb ischemia/reperfusion injury</title><author>Takhtfooladi, Mohammad Ashrafzadeh ; Moayer, Fariborz ; Takhtfooladi, Hamed Ashrafzadeh</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c528t-1d7fcb3cc864f61196c6d35c2726edf4cfebce4a8e94083d34d5584b843fc93b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Animals</topic><topic>Catalase - analysis</topic><topic>Disease Models, Animal</topic><topic>Free Radical Scavengers - pharmacology</topic><topic>Hindlimb</topic><topic>Hindlimb - blood supply</topic><topic>Ischemia</topic><topic>Ischemia - complications</topic><topic>Ischemia - prevention & control</topic><topic>Male</topic><topic>Malondialdehyde - analysis</topic><topic>Original</topic><topic>Oxidative Stress</topic><topic>Pentoxifylline</topic><topic>Pentoxifylline - pharmacology</topic><topic>Peroxidase - analysis</topic><topic>Random Allocation</topic><topic>Rats, Wistar</topic><topic>Reperfusion</topic><topic>Reperfusion Injury - complications</topic><topic>Reperfusion Injury - prevention & control</topic><topic>Reproducibility of Results</topic><topic>Superoxide Dismutase - analysis</topic><topic>Testicular Diseases</topic><topic>Testis - chemistry</topic><topic>Testis - drug effects</topic><topic>Testis - metabolism</topic><topic>Testis - pathology</topic><topic>Time Factors</topic><topic>Treatment Outcome</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Takhtfooladi, Mohammad Ashrafzadeh</creatorcontrib><creatorcontrib>Moayer, Fariborz</creatorcontrib><creatorcontrib>Takhtfooladi, Hamed Ashrafzadeh</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>International Brazilian Journal of Urology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Takhtfooladi, Mohammad Ashrafzadeh</au><au>Moayer, Fariborz</au><au>Takhtfooladi, Hamed Ashrafzadeh</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Beneficial effect of pentoxifylline into the testis of rats in an experimental model of unilateral hindlimb ischemia/reperfusion injury</atitle><jtitle>International Brazilian Journal of Urology</jtitle><addtitle>Int Braz J Urol</addtitle><date>2015-06-01</date><risdate>2015</risdate><volume>41</volume><issue>3</issue><spage>576</spage><epage>583</epage><pages>576-583</pages><issn>1677-6119</issn><issn>1677-5538</issn><eissn>1677-6119</eissn><abstract>The objective of the present study was to investigate the role of pentoxifylline (PTX) on remote testicular injury caused by unilateral hind limb ischemia/reperfusion of rats.
Twenty healthy male Wistar rats were allocated randomly into two groups: ischemia/reperfusion (IR group) and ischemia/reperfusion + pentoxifylline (IR+PTX group). Ischemia was induced by placement of a rubber tourniquet at the greater trochanter for 2h. Rats in IR+PTX group received PTX (40 mg/kg IP) before the reperfusion period. At 24h after reperfusion, testes were removed and levels of superoxide dismutase (SOD), malondialdehyde (MDA), catalase (CAT) and myeloperoxidase (MPO) activity were determined in testicular tissues. Three rats of each group were used for wet/ dry weight ratio measurement. Testicular tissues were also examined histopathologically under light microscopy.
Activities of SOD and CAT in testicular tissues were decreased by ischemia/ reperfusion (P<0.05). Significantly increased MDA levels in testicular tissues were decreased by PTX treatment (P<0.05). MPO activity in testicular tissues in the IR group was significantly higher than in the IR+PTX group (P<0.05). The wet/dry weight ratio of testicular tissues in the IR group was significantly higher than in the IR+PTX group (P<0.05). Histopathologically, there was a statistically significant difference between two groups (P<0.05).
According to histological and biochemical findings, we conclude that PTX has preventive effects in the testicular injury induced by hind limb ischemia/reperfusion.</abstract><cop>Brazil</cop><pub>Sociedade Brasileira de Urologia</pub><pmid>26200554</pmid><doi>10.1590/S1677-5538.IBJU.2014.0263</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1677-6119 |
| ispartof | International Brazilian Journal of Urology, 2015-06, Vol.41 (3), p.576-583 |
| issn | 1677-6119 1677-5538 1677-6119 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_4752154 |
| source | PubMed (Medline); MEDLINE; DOAJ Directory of Open Access Journals; EZB-FREE-00999 freely available EZB journals; PubMed Central Open Access |
| subjects | Animals Catalase - analysis Disease Models, Animal Free Radical Scavengers - pharmacology Hindlimb Hindlimb - blood supply Ischemia Ischemia - complications Ischemia - prevention & control Male Malondialdehyde - analysis Original Oxidative Stress Pentoxifylline Pentoxifylline - pharmacology Peroxidase - analysis Random Allocation Rats, Wistar Reperfusion Reperfusion Injury - complications Reperfusion Injury - prevention & control Reproducibility of Results Superoxide Dismutase - analysis Testicular Diseases Testis - chemistry Testis - drug effects Testis - metabolism Testis - pathology Time Factors Treatment Outcome |
| title | Beneficial effect of pentoxifylline into the testis of rats in an experimental model of unilateral hindlimb ischemia/reperfusion injury |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-25T14%3A24%3A11IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-pubmed_doaj_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Beneficial%20effect%20of%20pentoxifylline%20into%20the%20testis%20of%20rats%20in%20an%20experimental%20model%20of%20unilateral%20hindlimb%20ischemia/reperfusion%20injury&rft.jtitle=International%20Brazilian%20Journal%20of%20Urology&rft.au=Takhtfooladi,%20Mohammad%20Ashrafzadeh&rft.date=2015-06-01&rft.volume=41&rft.issue=3&rft.spage=576&rft.epage=583&rft.pages=576-583&rft.issn=1677-6119&rft.eissn=1677-6119&rft_id=info:doi/10.1590/S1677-5538.IBJU.2014.0263&rft_dat=%3Cpubmed_doaj_%3E26200554%3C/pubmed_doaj_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/26200554&rft_doaj_id=oai_doaj_org_article_d11251ec41214b2aa1d484f74d0f4e24&rfr_iscdi=true |