Effect of miR-146a/bFGF/PEG-PEI Nanoparticles on Inflammation Response and Tissue Regeneration of Human Dental Pulp Cells
Introduction. Inflammation in dental pulp cells (DPCs) initiated by Lipopolysaccharide (LPS) results in dental pulp necrosis. So far, whether there is a common system regulating inflammation response and tissue regeneration remains unknown. miR-146a is closely related to inflammation. Basic fibrobla...
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| description | Introduction. Inflammation in dental pulp cells (DPCs) initiated by Lipopolysaccharide (LPS) results in dental pulp necrosis. So far, whether there is a common system regulating inflammation response and tissue regeneration remains unknown. miR-146a is closely related to inflammation. Basic fibroblast growth factor (bFGF) is an important regulator for differentiation. Methods. To explore the effect of miR-146a/bFGF on inflammation and tissue regeneration, polyethylene glycol-polyethyleneimine (PEG-PEI) was synthesized, and physical characteristics were analyzed by dynamic light scattering and gel retardation analysis. Cell absorption, transfection efficiency, and cytotoxicity were assessed. Alginate gel was combined with miR-146a/PEG-PEI nanoparticles and bFGF. Drug release ratio was measured by ultraviolet spectrophotography. Proliferation and odontogenic differentiation of DPCs with 1 μg/mL LPS treatment were determined. Results. PEG-PEI prepared at N/P 2 showed complete gel retardation and smallest particle size and zeta potential. Transfection efficiency of PEG-PEI was higher than lipo2000. Cell viability decreased as N/P ratio increased. Drug release rate amounted to 70% at the first 12 h and then maintained slow release afterwards. Proliferation and differentiation decreased in DPCs with LPS treatment, whereas they increased in miR-146a/bFGF gel group. Conclusions. PEG-PEI is a promising vector for gene therapy. miR-146a and bFGF play critical roles in inflammation response and tissue regeneration of DPCs. |
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| fullrecord | <record><control><sourceid>gale_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_4745861</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A520714923</galeid><sourcerecordid>A520714923</sourcerecordid><originalsourceid>FETCH-LOGICAL-c532t-e93122380c0c5c9c706a142dd429677a7e54e473dae5a98114b1aabcf277519e3</originalsourceid><addsrcrecordid>eNqN0s9v0zAUB_AIgdhUduOMInFBGqH-GccXpKm0XaUJqmmcrVfnpfOU2CVOQPvvcdVSBqf54l8ffWU_vSx7S8knSqWcMkLLKa80Kyv5IjtnnIqipIK-PK05P8suYnwgaVS0JLp8nZ0xRaSSgpxnj_OmQTvkock7d1tQUcJ0s1gupuv5sljPV_lX8GEH_eBsizEPPl_5poWug8GlzS3GXfARc_B1fudiHDGdbdFjfwAp93rswOdf0A_Q5uux3eUzbNv4JnvVQBvx4jhPsu-L-d3surj5tlzNrm4KKzkbCtScMsYrYomVVltFSqCC1bVgulQKFEqBQvEaUIKuKBUbCrCxDVNKUo18kn0-5O7GTYe1Te_ooTW73nXQP5oAzvx749292YafRighq1TASfbhGNCHHyPGwXQu2vQF8BjGaKiqiKRMU_0MWkqtmapkou__ow9h7H2qxF4xzQmj9K_aQovG-SakJ9p9qLmSjCgqNONJfTwo24cYe2xOv6PE7PvE7PvEHPsk8XdPK3LCf7oigcsDuHe-hl_umXGYDDbwRGslqor_Brlly3w</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1762930211</pqid></control><display><type>article</type><title>Effect of miR-146a/bFGF/PEG-PEI Nanoparticles on Inflammation Response and Tissue Regeneration of Human Dental Pulp Cells</title><source>MEDLINE</source><source>PubMed Central Open Access</source><source>Wiley-Blackwell Open Access Titles</source><source>PubMed Central</source><source>Alma/SFX Local Collection</source><creator>Wei, Xi ; Cheung, Gary Shunpan ; Shu, Shan ; Liu, Lu</creator><contributor>Safe, Stephen H.</contributor><creatorcontrib>Wei, Xi ; Cheung, Gary Shunpan ; Shu, Shan ; Liu, Lu ; Safe, Stephen H.</creatorcontrib><description>Introduction. Inflammation in dental pulp cells (DPCs) initiated by Lipopolysaccharide (LPS) results in dental pulp necrosis. So far, whether there is a common system regulating inflammation response and tissue regeneration remains unknown. miR-146a is closely related to inflammation. Basic fibroblast growth factor (bFGF) is an important regulator for differentiation. Methods. To explore the effect of miR-146a/bFGF on inflammation and tissue regeneration, polyethylene glycol-polyethyleneimine (PEG-PEI) was synthesized, and physical characteristics were analyzed by dynamic light scattering and gel retardation analysis. Cell absorption, transfection efficiency, and cytotoxicity were assessed. Alginate gel was combined with miR-146a/PEG-PEI nanoparticles and bFGF. Drug release ratio was measured by ultraviolet spectrophotography. Proliferation and odontogenic differentiation of DPCs with 1 μg/mL LPS treatment were determined. Results. PEG-PEI prepared at N/P 2 showed complete gel retardation and smallest particle size and zeta potential. Transfection efficiency of PEG-PEI was higher than lipo2000. Cell viability decreased as N/P ratio increased. Drug release rate amounted to 70% at the first 12 h and then maintained slow release afterwards. Proliferation and differentiation decreased in DPCs with LPS treatment, whereas they increased in miR-146a/bFGF gel group. Conclusions. PEG-PEI is a promising vector for gene therapy. miR-146a and bFGF play critical roles in inflammation response and tissue regeneration of DPCs.</description><identifier>ISSN: 2314-6133</identifier><identifier>EISSN: 2314-6141</identifier><identifier>DOI: 10.1155/2016/3892685</identifier><identifier>PMID: 27057540</identifier><language>eng</language><publisher>Cairo, Egypt: Hindawi Publishing Corporation</publisher><subject>Adolescent ; Adult ; Analysis ; Biocompatibility ; Cell growth ; Cell Proliferation - drug effects ; Cytokines ; Cytotoxicity ; Dental Pulp - cytology ; Dentistry ; Drug Carriers - chemistry ; Efficiency ; Fibroblast Growth Factor 2 - chemistry ; Fibroblast Growth Factor 2 - pharmacokinetics ; Fibroblast Growth Factor 2 - pharmacology ; Fibroblast growth factors ; Fourier transforms ; Gene therapy ; Genetic vectors ; Humans ; Hydrogels ; Infection ; Inflammation ; Lipopolysaccharides - adverse effects ; MicroRNAs - chemistry ; MicroRNAs - pharmacokinetics ; MicroRNAs - pharmacology ; Mitogens ; Nanoparticles ; Nanoparticles - chemistry ; Polyethylene glycol ; Polyethylene Glycols - chemistry ; Polyethyleneimine - chemistry ; Polymer solubility ; Polyols ; Proteins ; Pulpitis - chemically induced ; Pulpitis - metabolism ; Regeneration - drug effects ; Tissue engineering ; Vectors (Biology) ; Wound healing ; Young Adult</subject><ispartof>BioMed research international, 2016-01, Vol.2016 (2016), p.1-12</ispartof><rights>Copyright © 2016 Lu Liu et al.</rights><rights>COPYRIGHT 2016 John Wiley & Sons, Inc.</rights><rights>Copyright © 2016 Lu Liu et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.</rights><rights>Copyright © 2016 Lu Liu et al. 2016</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c532t-e93122380c0c5c9c706a142dd429677a7e54e473dae5a98114b1aabcf277519e3</citedby><cites>FETCH-LOGICAL-c532t-e93122380c0c5c9c706a142dd429677a7e54e473dae5a98114b1aabcf277519e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4745861/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4745861/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,27903,27904,53769,53771</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27057540$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Safe, Stephen H.</contributor><creatorcontrib>Wei, Xi</creatorcontrib><creatorcontrib>Cheung, Gary Shunpan</creatorcontrib><creatorcontrib>Shu, Shan</creatorcontrib><creatorcontrib>Liu, Lu</creatorcontrib><title>Effect of miR-146a/bFGF/PEG-PEI Nanoparticles on Inflammation Response and Tissue Regeneration of Human Dental Pulp Cells</title><title>BioMed research international</title><addtitle>Biomed Res Int</addtitle><description>Introduction. Inflammation in dental pulp cells (DPCs) initiated by Lipopolysaccharide (LPS) results in dental pulp necrosis. So far, whether there is a common system regulating inflammation response and tissue regeneration remains unknown. miR-146a is closely related to inflammation. Basic fibroblast growth factor (bFGF) is an important regulator for differentiation. Methods. To explore the effect of miR-146a/bFGF on inflammation and tissue regeneration, polyethylene glycol-polyethyleneimine (PEG-PEI) was synthesized, and physical characteristics were analyzed by dynamic light scattering and gel retardation analysis. Cell absorption, transfection efficiency, and cytotoxicity were assessed. Alginate gel was combined with miR-146a/PEG-PEI nanoparticles and bFGF. Drug release ratio was measured by ultraviolet spectrophotography. Proliferation and odontogenic differentiation of DPCs with 1 μg/mL LPS treatment were determined. Results. PEG-PEI prepared at N/P 2 showed complete gel retardation and smallest particle size and zeta potential. Transfection efficiency of PEG-PEI was higher than lipo2000. Cell viability decreased as N/P ratio increased. Drug release rate amounted to 70% at the first 12 h and then maintained slow release afterwards. Proliferation and differentiation decreased in DPCs with LPS treatment, whereas they increased in miR-146a/bFGF gel group. Conclusions. PEG-PEI is a promising vector for gene therapy. miR-146a and bFGF play critical roles in inflammation response and tissue regeneration of DPCs.</description><subject>Adolescent</subject><subject>Adult</subject><subject>Analysis</subject><subject>Biocompatibility</subject><subject>Cell growth</subject><subject>Cell Proliferation - drug effects</subject><subject>Cytokines</subject><subject>Cytotoxicity</subject><subject>Dental Pulp - cytology</subject><subject>Dentistry</subject><subject>Drug Carriers - chemistry</subject><subject>Efficiency</subject><subject>Fibroblast Growth Factor 2 - chemistry</subject><subject>Fibroblast Growth Factor 2 - pharmacokinetics</subject><subject>Fibroblast Growth Factor 2 - pharmacology</subject><subject>Fibroblast growth factors</subject><subject>Fourier transforms</subject><subject>Gene therapy</subject><subject>Genetic vectors</subject><subject>Humans</subject><subject>Hydrogels</subject><subject>Infection</subject><subject>Inflammation</subject><subject>Lipopolysaccharides - adverse effects</subject><subject>MicroRNAs - chemistry</subject><subject>MicroRNAs - pharmacokinetics</subject><subject>MicroRNAs - pharmacology</subject><subject>Mitogens</subject><subject>Nanoparticles</subject><subject>Nanoparticles - chemistry</subject><subject>Polyethylene glycol</subject><subject>Polyethylene Glycols - chemistry</subject><subject>Polyethyleneimine - chemistry</subject><subject>Polymer solubility</subject><subject>Polyols</subject><subject>Proteins</subject><subject>Pulpitis - chemically induced</subject><subject>Pulpitis - metabolism</subject><subject>Regeneration - drug effects</subject><subject>Tissue engineering</subject><subject>Vectors (Biology)</subject><subject>Wound healing</subject><subject>Young Adult</subject><issn>2314-6133</issn><issn>2314-6141</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>RHX</sourceid><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqN0s9v0zAUB_AIgdhUduOMInFBGqH-GccXpKm0XaUJqmmcrVfnpfOU2CVOQPvvcdVSBqf54l8ffWU_vSx7S8knSqWcMkLLKa80Kyv5IjtnnIqipIK-PK05P8suYnwgaVS0JLp8nZ0xRaSSgpxnj_OmQTvkock7d1tQUcJ0s1gupuv5sljPV_lX8GEH_eBsizEPPl_5poWug8GlzS3GXfARc_B1fudiHDGdbdFjfwAp93rswOdf0A_Q5uux3eUzbNv4JnvVQBvx4jhPsu-L-d3surj5tlzNrm4KKzkbCtScMsYrYomVVltFSqCC1bVgulQKFEqBQvEaUIKuKBUbCrCxDVNKUo18kn0-5O7GTYe1Te_ooTW73nXQP5oAzvx749292YafRighq1TASfbhGNCHHyPGwXQu2vQF8BjGaKiqiKRMU_0MWkqtmapkou__ow9h7H2qxF4xzQmj9K_aQovG-SakJ9p9qLmSjCgqNONJfTwo24cYe2xOv6PE7PvE7PvEHPsk8XdPK3LCf7oigcsDuHe-hl_umXGYDDbwRGslqor_Brlly3w</recordid><startdate>20160101</startdate><enddate>20160101</enddate><creator>Wei, Xi</creator><creator>Cheung, Gary Shunpan</creator><creator>Shu, Shan</creator><creator>Liu, Lu</creator><general>Hindawi Publishing Corporation</general><general>John Wiley & Sons, Inc</general><general>Hindawi Limited</general><scope>ADJCN</scope><scope>AHFXO</scope><scope>RHU</scope><scope>RHW</scope><scope>RHX</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7QO</scope><scope>7T7</scope><scope>7TK</scope><scope>7U7</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>CWDGH</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20160101</creationdate><title>Effect of miR-146a/bFGF/PEG-PEI Nanoparticles on Inflammation Response and Tissue Regeneration of Human Dental Pulp Cells</title><author>Wei, Xi ; Cheung, Gary Shunpan ; Shu, Shan ; Liu, Lu</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c532t-e93122380c0c5c9c706a142dd429677a7e54e473dae5a98114b1aabcf277519e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Adolescent</topic><topic>Adult</topic><topic>Analysis</topic><topic>Biocompatibility</topic><topic>Cell growth</topic><topic>Cell Proliferation - drug effects</topic><topic>Cytokines</topic><topic>Cytotoxicity</topic><topic>Dental Pulp - cytology</topic><topic>Dentistry</topic><topic>Drug Carriers - chemistry</topic><topic>Efficiency</topic><topic>Fibroblast Growth Factor 2 - chemistry</topic><topic>Fibroblast Growth Factor 2 - pharmacokinetics</topic><topic>Fibroblast Growth Factor 2 - pharmacology</topic><topic>Fibroblast growth factors</topic><topic>Fourier transforms</topic><topic>Gene therapy</topic><topic>Genetic vectors</topic><topic>Humans</topic><topic>Hydrogels</topic><topic>Infection</topic><topic>Inflammation</topic><topic>Lipopolysaccharides - adverse effects</topic><topic>MicroRNAs - chemistry</topic><topic>MicroRNAs - pharmacokinetics</topic><topic>MicroRNAs - pharmacology</topic><topic>Mitogens</topic><topic>Nanoparticles</topic><topic>Nanoparticles - chemistry</topic><topic>Polyethylene glycol</topic><topic>Polyethylene Glycols - chemistry</topic><topic>Polyethyleneimine - chemistry</topic><topic>Polymer solubility</topic><topic>Polyols</topic><topic>Proteins</topic><topic>Pulpitis - chemically induced</topic><topic>Pulpitis - metabolism</topic><topic>Regeneration - drug effects</topic><topic>Tissue engineering</topic><topic>Vectors (Biology)</topic><topic>Wound healing</topic><topic>Young Adult</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wei, Xi</creatorcontrib><creatorcontrib>Cheung, Gary Shunpan</creatorcontrib><creatorcontrib>Shu, Shan</creatorcontrib><creatorcontrib>Liu, Lu</creatorcontrib><collection>الدوريات العلمية والإحصائية - e-Marefa Academic and Statistical Periodicals</collection><collection>معرفة - المحتوى العربي الأكاديمي المتكامل - e-Marefa Academic Complete</collection><collection>Hindawi Publishing Complete</collection><collection>Hindawi Publishing Subscription Journals</collection><collection>Hindawi Publishing Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Neurosciences Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies & Aerospace Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>Middle East & Africa Database</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Advanced Technologies & Aerospace Database</collection><collection>ProQuest Advanced Technologies & Aerospace Collection</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>BioMed research international</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wei, Xi</au><au>Cheung, Gary Shunpan</au><au>Shu, Shan</au><au>Liu, Lu</au><au>Safe, Stephen H.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of miR-146a/bFGF/PEG-PEI Nanoparticles on Inflammation Response and Tissue Regeneration of Human Dental Pulp Cells</atitle><jtitle>BioMed research international</jtitle><addtitle>Biomed Res Int</addtitle><date>2016-01-01</date><risdate>2016</risdate><volume>2016</volume><issue>2016</issue><spage>1</spage><epage>12</epage><pages>1-12</pages><issn>2314-6133</issn><eissn>2314-6141</eissn><abstract>Introduction. Inflammation in dental pulp cells (DPCs) initiated by Lipopolysaccharide (LPS) results in dental pulp necrosis. So far, whether there is a common system regulating inflammation response and tissue regeneration remains unknown. miR-146a is closely related to inflammation. Basic fibroblast growth factor (bFGF) is an important regulator for differentiation. Methods. To explore the effect of miR-146a/bFGF on inflammation and tissue regeneration, polyethylene glycol-polyethyleneimine (PEG-PEI) was synthesized, and physical characteristics were analyzed by dynamic light scattering and gel retardation analysis. Cell absorption, transfection efficiency, and cytotoxicity were assessed. Alginate gel was combined with miR-146a/PEG-PEI nanoparticles and bFGF. Drug release ratio was measured by ultraviolet spectrophotography. Proliferation and odontogenic differentiation of DPCs with 1 μg/mL LPS treatment were determined. Results. PEG-PEI prepared at N/P 2 showed complete gel retardation and smallest particle size and zeta potential. Transfection efficiency of PEG-PEI was higher than lipo2000. Cell viability decreased as N/P ratio increased. Drug release rate amounted to 70% at the first 12 h and then maintained slow release afterwards. Proliferation and differentiation decreased in DPCs with LPS treatment, whereas they increased in miR-146a/bFGF gel group. Conclusions. PEG-PEI is a promising vector for gene therapy. miR-146a and bFGF play critical roles in inflammation response and tissue regeneration of DPCs.</abstract><cop>Cairo, Egypt</cop><pub>Hindawi Publishing Corporation</pub><pmid>27057540</pmid><doi>10.1155/2016/3892685</doi><tpages>12</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Adolescent Adult Analysis Biocompatibility Cell growth Cell Proliferation - drug effects Cytokines Cytotoxicity Dental Pulp - cytology Dentistry Drug Carriers - chemistry Efficiency Fibroblast Growth Factor 2 - chemistry Fibroblast Growth Factor 2 - pharmacokinetics Fibroblast Growth Factor 2 - pharmacology Fibroblast growth factors Fourier transforms Gene therapy Genetic vectors Humans Hydrogels Infection Inflammation Lipopolysaccharides - adverse effects MicroRNAs - chemistry MicroRNAs - pharmacokinetics MicroRNAs - pharmacology Mitogens Nanoparticles Nanoparticles - chemistry Polyethylene glycol Polyethylene Glycols - chemistry Polyethyleneimine - chemistry Polymer solubility Polyols Proteins Pulpitis - chemically induced Pulpitis - metabolism Regeneration - drug effects Tissue engineering Vectors (Biology) Wound healing Young Adult |
| title | Effect of miR-146a/bFGF/PEG-PEI Nanoparticles on Inflammation Response and Tissue Regeneration of Human Dental Pulp Cells |
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