No SEVI-mediated enhancement of rectal HIV-1 transmission of HIV-1 in two humanized mouse cohorts
Abstract Amyloid fibrils from semen-derived peptide (SEVI) enhance HIV-1 infectivity in vitro but the ability of SEVI to mediate enhancement of HIV infection in vivo has not been tested. In this study we used immunodeficient mice reconstituted with human immune systems to test for in vivo enhancemen...
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| Veröffentlicht in: | Virology (New York, N.Y.) N.Y.), 2016-01, Vol.488, p.88-95 |
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| container_title | Virology (New York, N.Y.) |
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| creator | Dis, Erik S. Van Moore, Tyler C Lavender, Kerry J Messer, Ronald J Keppler, Oliver T Verheyen, Jens Dittmer, Ulf Hasenkrug, Kim J |
| description | Abstract Amyloid fibrils from semen-derived peptide (SEVI) enhance HIV-1 infectivity in vitro but the ability of SEVI to mediate enhancement of HIV infection in vivo has not been tested. In this study we used immunodeficient mice reconstituted with human immune systems to test for in vivo enhancement of HIV-1 transmission. This mouse model supports mucosal transmission of HIV-1 via the intrarectal route leading to productive infection. In separate experiments with humanized mouse cohorts reconstituted with two different donor immune systems, high dose HIV-1JR-CSF that had been incubated with SEVI amyloid fibrils at physiologically relevant concentrations did not show an increased incidence of infection compared to controls. In addition, SEVI failed to enhance rectal transmission with a reduced concentration of HIV-1. Although we confirmed potent SEVI-mediated enhancement of HIV infectivity in vitro , this model showed no evidence that it plays a role in the much more complex situation of in vivo transmission. |
| doi_str_mv | 10.1016/j.virol.2015.11.005 |
| format | Article |
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Van ; Moore, Tyler C ; Lavender, Kerry J ; Messer, Ronald J ; Keppler, Oliver T ; Verheyen, Jens ; Dittmer, Ulf ; Hasenkrug, Kim J</creator><creatorcontrib>Dis, Erik S. Van ; Moore, Tyler C ; Lavender, Kerry J ; Messer, Ronald J ; Keppler, Oliver T ; Verheyen, Jens ; Dittmer, Ulf ; Hasenkrug, Kim J</creatorcontrib><description>Abstract Amyloid fibrils from semen-derived peptide (SEVI) enhance HIV-1 infectivity in vitro but the ability of SEVI to mediate enhancement of HIV infection in vivo has not been tested. In this study we used immunodeficient mice reconstituted with human immune systems to test for in vivo enhancement of HIV-1 transmission. This mouse model supports mucosal transmission of HIV-1 via the intrarectal route leading to productive infection. In separate experiments with humanized mouse cohorts reconstituted with two different donor immune systems, high dose HIV-1JR-CSF that had been incubated with SEVI amyloid fibrils at physiologically relevant concentrations did not show an increased incidence of infection compared to controls. In addition, SEVI failed to enhance rectal transmission with a reduced concentration of HIV-1. Although we confirmed potent SEVI-mediated enhancement of HIV infectivity in vitro , this model showed no evidence that it plays a role in the much more complex situation of in vivo transmission.</description><identifier>ISSN: 0042-6822</identifier><identifier>EISSN: 1096-0341</identifier><identifier>DOI: 10.1016/j.virol.2015.11.005</identifier><identifier>PMID: 26609939</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Amyloid ; Amyloid - metabolism ; Animals ; CD4-Positive T-Lymphocytes - virology ; Cells, Cultured ; Disease Models, Animal ; Female ; HIV ; HIV Infections - transmission ; Human immunodeficiency virus ; Human immunodeficiency virus 1 ; Humanized mice ; Humans ; Incidence ; Infectious Disease ; Male ; Mice, Inbred C57BL ; Mice, SCID ; Rectum - virology ; Semen - chemistry ; Semen-derived enhancer of viral infection ; SEVI ; Transmission</subject><ispartof>Virology (New York, N.Y.), 2016-01, Vol.488, p.88-95</ispartof><rights>2015</rights><rights>Published by Elsevier Inc.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c547t-1afaf7fc04a34cc689503a6cbc4f7271941b3bae45ec2247316261ea6ea8b9f43</citedby><cites>FETCH-LOGICAL-c547t-1afaf7fc04a34cc689503a6cbc4f7271941b3bae45ec2247316261ea6ea8b9f43</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0042682215004687$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>230,314,776,780,881,3537,27901,27902,65534</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26609939$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Dis, Erik S. Van</creatorcontrib><creatorcontrib>Moore, Tyler C</creatorcontrib><creatorcontrib>Lavender, Kerry J</creatorcontrib><creatorcontrib>Messer, Ronald J</creatorcontrib><creatorcontrib>Keppler, Oliver T</creatorcontrib><creatorcontrib>Verheyen, Jens</creatorcontrib><creatorcontrib>Dittmer, Ulf</creatorcontrib><creatorcontrib>Hasenkrug, Kim J</creatorcontrib><title>No SEVI-mediated enhancement of rectal HIV-1 transmission of HIV-1 in two humanized mouse cohorts</title><title>Virology (New York, N.Y.)</title><addtitle>Virology</addtitle><description>Abstract Amyloid fibrils from semen-derived peptide (SEVI) enhance HIV-1 infectivity in vitro but the ability of SEVI to mediate enhancement of HIV infection in vivo has not been tested. In this study we used immunodeficient mice reconstituted with human immune systems to test for in vivo enhancement of HIV-1 transmission. This mouse model supports mucosal transmission of HIV-1 via the intrarectal route leading to productive infection. In separate experiments with humanized mouse cohorts reconstituted with two different donor immune systems, high dose HIV-1JR-CSF that had been incubated with SEVI amyloid fibrils at physiologically relevant concentrations did not show an increased incidence of infection compared to controls. In addition, SEVI failed to enhance rectal transmission with a reduced concentration of HIV-1. Although we confirmed potent SEVI-mediated enhancement of HIV infectivity in vitro , this model showed no evidence that it plays a role in the much more complex situation of in vivo transmission.</description><subject>Amyloid</subject><subject>Amyloid - metabolism</subject><subject>Animals</subject><subject>CD4-Positive T-Lymphocytes - virology</subject><subject>Cells, Cultured</subject><subject>Disease Models, Animal</subject><subject>Female</subject><subject>HIV</subject><subject>HIV Infections - transmission</subject><subject>Human immunodeficiency virus</subject><subject>Human immunodeficiency virus 1</subject><subject>Humanized mice</subject><subject>Humans</subject><subject>Incidence</subject><subject>Infectious Disease</subject><subject>Male</subject><subject>Mice, Inbred C57BL</subject><subject>Mice, SCID</subject><subject>Rectum - virology</subject><subject>Semen - chemistry</subject><subject>Semen-derived enhancer of viral infection</subject><subject>SEVI</subject><subject>Transmission</subject><issn>0042-6822</issn><issn>1096-0341</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNUk1v1DAQtRCIbgu_AAnlyCXBYztOfKASqkq7UgWHQq-W452wXhK72Mmi8utx2FIBFzj5Y968mTdvCHkBtAIK8vWu2rsYhopRqCuAitL6EVkBVbKkXMBjsqJUsFK2jB2R45R2NL-bhj4lR0xKqhRXK2Leh-L6_GZdjrhxZsJNgX5rvMUR_VSEvohoJzMUl-ubEoopGp9Gl5ILfgkefp0vpm-h2M6j8e57phjDnLCwYRvilJ6RJ70ZEj6_P0_Ip3fnH88uy6sPF-uzt1elrUUzlWB60ze9pcJwYa1sVU25kbazom9YA0pAxzuDokbLmGg4SCYBjUTTdqoX_IScHnhv5y6Lsbn_aAZ9G91o4p0Oxuk_I95t9eew16IRoqYqE7y6J4jh64xp0lmpxWEwHrMgDY3Ks811-X9AJVN1q4BlKD9AbQwpRewfOgKqFx_1Tv_0US8-agCdfcxZL38X85Dzy7gMeHMAYB7p3mHUyTrMvm3c4pjeBPePAqd_5dvBeWfN8AXvMO3CHH12S4NOTFN9vazSsklQ55tsG_4DjVPE3A</recordid><startdate>20160115</startdate><enddate>20160115</enddate><creator>Dis, Erik S. Van</creator><creator>Moore, Tyler C</creator><creator>Lavender, Kerry J</creator><creator>Messer, Ronald J</creator><creator>Keppler, Oliver T</creator><creator>Verheyen, Jens</creator><creator>Dittmer, Ulf</creator><creator>Hasenkrug, Kim J</creator><general>Elsevier Inc</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7U9</scope><scope>H94</scope><scope>5PM</scope></search><sort><creationdate>20160115</creationdate><title>No SEVI-mediated enhancement of rectal HIV-1 transmission of HIV-1 in two humanized mouse cohorts</title><author>Dis, Erik S. Van ; Moore, Tyler C ; Lavender, Kerry J ; Messer, Ronald J ; Keppler, Oliver T ; Verheyen, Jens ; Dittmer, Ulf ; Hasenkrug, Kim J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c547t-1afaf7fc04a34cc689503a6cbc4f7271941b3bae45ec2247316261ea6ea8b9f43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Amyloid</topic><topic>Amyloid - metabolism</topic><topic>Animals</topic><topic>CD4-Positive T-Lymphocytes - virology</topic><topic>Cells, Cultured</topic><topic>Disease Models, Animal</topic><topic>Female</topic><topic>HIV</topic><topic>HIV Infections - transmission</topic><topic>Human immunodeficiency virus</topic><topic>Human immunodeficiency virus 1</topic><topic>Humanized mice</topic><topic>Humans</topic><topic>Incidence</topic><topic>Infectious Disease</topic><topic>Male</topic><topic>Mice, Inbred C57BL</topic><topic>Mice, SCID</topic><topic>Rectum - virology</topic><topic>Semen - chemistry</topic><topic>Semen-derived enhancer of viral infection</topic><topic>SEVI</topic><topic>Transmission</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Dis, Erik S. Van</creatorcontrib><creatorcontrib>Moore, Tyler C</creatorcontrib><creatorcontrib>Lavender, Kerry J</creatorcontrib><creatorcontrib>Messer, Ronald J</creatorcontrib><creatorcontrib>Keppler, Oliver T</creatorcontrib><creatorcontrib>Verheyen, Jens</creatorcontrib><creatorcontrib>Dittmer, Ulf</creatorcontrib><creatorcontrib>Hasenkrug, Kim J</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Virology (New York, N.Y.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Dis, Erik S. 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| identifier | ISSN: 0042-6822 |
| ispartof | Virology (New York, N.Y.), 2016-01, Vol.488, p.88-95 |
| issn | 0042-6822 1096-0341 |
| language | eng |
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| source | MEDLINE; ScienceDirect Journals (5 years ago - present); Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | Amyloid Amyloid - metabolism Animals CD4-Positive T-Lymphocytes - virology Cells, Cultured Disease Models, Animal Female HIV HIV Infections - transmission Human immunodeficiency virus Human immunodeficiency virus 1 Humanized mice Humans Incidence Infectious Disease Male Mice, Inbred C57BL Mice, SCID Rectum - virology Semen - chemistry Semen-derived enhancer of viral infection SEVI Transmission |
| title | No SEVI-mediated enhancement of rectal HIV-1 transmission of HIV-1 in two humanized mouse cohorts |
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