Immunochemical Detection of 4-Hydroxynonenal Protein Adducts in Oxidized Hepatocytes

We report here the development of an immunochemical procedure that uses an antibody specific to the 4-hydroxynonenal (HNE) moiety for the detection of HNE-protein adducts. The HNE-specific antibody was prepared by immunizing rabbits with a HNE-keyhole limpet hemocyanin conjugate and purifying the ra...

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Veröffentlicht in:	Proceedings of the National Academy of Sciences - PNAS 1993-09, Vol.90 (18), p.8742-8746
Hauptverfasser:	Uchida, Koji, Szweda, Luke I., Chae, Ho-Zoon, Stadtman, Earl R.
Format:	Artikel
Sprache:	eng
Schlagworte:	Adducts Aldehydes - analysis Aldehydes - metabolism Amino Acid Sequence Amino acids Animals Antibodies Biochemistry Biological and medical sciences Cell metabolism, cell oxidation Cell physiology Cells, Cultured Electrophoresis, Polyacrylamide Gel Enzyme-Linked Immunosorbent Assay Epitopes Fundamental and applied biological sciences. Psychology Hepatocytes Histidine - metabolism Immunity (Disease) Immunoblotting Immunohistochemistry - methods Kinetics Lipid Peroxidation Lipids Liver Liver - metabolism Medical research Molecular and cellular biology Molecular Sequence Data Oligopeptides - chemical synthesis Oligopeptides - metabolism Oxidation Phosphates Protein Binding Proteins Proteins - analysis Proteins - metabolism Rats Rats, Inbred BN Rats, Inbred F344
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