Developmental expression and cardiac transcriptional regulation of Myh7b, a third myosin heavy chain in the vertebrate heart
The mammalian heart expresses two myosin heavy chain (MYH) genes (Myh6 and Myh7), which are major components of the thick filaments of the sarcomere. We have determined that a third MYH, MYH7B, is also expressed in the myocardium. Developmental analysis shows Myh7b expression in cardiac and skeletal...
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| Veröffentlicht in: | Cytoskeleton (Hoboken, N.J.) N.J.), 2012-05, Vol.69 (5), p.324-335 |
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| creator | Warkman, Andrew S. Whitman, Samantha A. Miller, Melanie K. Garriock, Robert J. Schwach, Catherine M. Gregorio, Carol C. Krieg, Paul A. |
| description | The mammalian heart expresses two myosin heavy chain (MYH) genes (Myh6 and Myh7), which are major components of the thick filaments of the sarcomere. We have determined that a third MYH, MYH7B, is also expressed in the myocardium. Developmental analysis shows Myh7b expression in cardiac and skeletal muscle of Xenopus, chick and mouse embryos, and in smooth muscle tissues during later stages of mouse embryogenesis. Myh7b is also expressed in the adult human heart. The promoter region of the Myh7b gene shows remarkable similarity between diverse species, suggesting that transcriptional control mechanisms have been conserved. Using luciferase reporter analysis in rat cardiomyocytes, it can be shown that MEF2, GATA, and E‐box regulatory elements are essential for efficient expression of the Myh7b gene. In addition two conserved elements that do not correspond to consensus binding sites for known transcription factors are also essential for full transcriptional activity of the Myh7b reporter. Finally, the Myh7b gene shows a transcriptional response similar to Myh6 in response to cardiac hypertrophy. © 2012 Wiley Periodicals, Inc |
| doi_str_mv | 10.1002/cm.21029 |
| format | Article |
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We have determined that a third MYH, MYH7B, is also expressed in the myocardium. Developmental analysis shows Myh7b expression in cardiac and skeletal muscle of Xenopus, chick and mouse embryos, and in smooth muscle tissues during later stages of mouse embryogenesis. Myh7b is also expressed in the adult human heart. The promoter region of the Myh7b gene shows remarkable similarity between diverse species, suggesting that transcriptional control mechanisms have been conserved. Using luciferase reporter analysis in rat cardiomyocytes, it can be shown that MEF2, GATA, and E‐box regulatory elements are essential for efficient expression of the Myh7b gene. In addition two conserved elements that do not correspond to consensus binding sites for known transcription factors are also essential for full transcriptional activity of the Myh7b reporter. Finally, the Myh7b gene shows a transcriptional response similar to Myh6 in response to cardiac hypertrophy. © 2012 Wiley Periodicals, Inc</description><identifier>ISSN: 1949-3584</identifier><identifier>EISSN: 1949-3592</identifier><identifier>DOI: 10.1002/cm.21029</identifier><identifier>PMID: 22422726</identifier><language>eng</language><publisher>Hoboken, USA: John Wiley & Sons, Inc</publisher><subject>Adult ; Amino Acid Sequence ; Animals ; Base Sequence ; Binding Sites ; Blotting, Western ; cardiac development ; Cardiomegaly - genetics ; Cardiomegaly - metabolism ; Cardiomegaly - pathology ; Cell Nucleus - metabolism ; Cells, Cultured ; Chick Embryo ; DNA-Binding Proteins - genetics ; DNA-Binding Proteins - metabolism ; Gene Expression Regulation, Developmental ; Heart - embryology ; Heart - physiology ; Humans ; hypertrophy ; Immunoenzyme Techniques ; In Situ Hybridization ; Luciferases - metabolism ; Mice - embryology ; Molecular Sequence Data ; Muscle, Skeletal - cytology ; Muscle, Skeletal - metabolism ; Myocardium - cytology ; Myocardium - metabolism ; myosin heavy chain ; Myosin Heavy Chains - genetics ; Myosin Heavy Chains - metabolism ; promoter analysis ; Promoter Regions, Genetic - genetics ; Rats ; Real-Time Polymerase Chain Reaction ; Reverse Transcriptase Polymerase Chain Reaction ; RNA, Messenger - genetics ; Sequence Homology, Amino Acid ; Sequence Homology, Nucleic Acid ; smooth muscle ; somites ; Transcription Factors - genetics ; Transcription Factors - metabolism ; Transcription, Genetic ; Transcriptional Activation ; Xenopus laevis - embryology</subject><ispartof>Cytoskeleton (Hoboken, N.J.), 2012-05, Vol.69 (5), p.324-335</ispartof><rights>Copyright © 2012 Wiley Periodicals, Inc.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5149-c8df70fb544b187f508d78365959d43d301132cd94c7e4a643733fcfd73d94353</citedby><cites>FETCH-LOGICAL-c5149-c8df70fb544b187f508d78365959d43d301132cd94c7e4a643733fcfd73d94353</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fcm.21029$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fcm.21029$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>230,314,780,784,885,1416,27922,27923,45572,45573</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22422726$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Warkman, Andrew S.</creatorcontrib><creatorcontrib>Whitman, Samantha A.</creatorcontrib><creatorcontrib>Miller, Melanie K.</creatorcontrib><creatorcontrib>Garriock, Robert J.</creatorcontrib><creatorcontrib>Schwach, Catherine M.</creatorcontrib><creatorcontrib>Gregorio, Carol C.</creatorcontrib><creatorcontrib>Krieg, Paul A.</creatorcontrib><title>Developmental expression and cardiac transcriptional regulation of Myh7b, a third myosin heavy chain in the vertebrate heart</title><title>Cytoskeleton (Hoboken, N.J.)</title><addtitle>Cytoskeleton</addtitle><description>The mammalian heart expresses two myosin heavy chain (MYH) genes (Myh6 and Myh7), which are major components of the thick filaments of the sarcomere. We have determined that a third MYH, MYH7B, is also expressed in the myocardium. Developmental analysis shows Myh7b expression in cardiac and skeletal muscle of Xenopus, chick and mouse embryos, and in smooth muscle tissues during later stages of mouse embryogenesis. Myh7b is also expressed in the adult human heart. The promoter region of the Myh7b gene shows remarkable similarity between diverse species, suggesting that transcriptional control mechanisms have been conserved. Using luciferase reporter analysis in rat cardiomyocytes, it can be shown that MEF2, GATA, and E‐box regulatory elements are essential for efficient expression of the Myh7b gene. In addition two conserved elements that do not correspond to consensus binding sites for known transcription factors are also essential for full transcriptional activity of the Myh7b reporter. Finally, the Myh7b gene shows a transcriptional response similar to Myh6 in response to cardiac hypertrophy. © 2012 Wiley Periodicals, Inc</description><subject>Adult</subject><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Base Sequence</subject><subject>Binding Sites</subject><subject>Blotting, Western</subject><subject>cardiac development</subject><subject>Cardiomegaly - genetics</subject><subject>Cardiomegaly - metabolism</subject><subject>Cardiomegaly - pathology</subject><subject>Cell Nucleus - metabolism</subject><subject>Cells, Cultured</subject><subject>Chick Embryo</subject><subject>DNA-Binding Proteins - genetics</subject><subject>DNA-Binding Proteins - metabolism</subject><subject>Gene Expression Regulation, Developmental</subject><subject>Heart - embryology</subject><subject>Heart - physiology</subject><subject>Humans</subject><subject>hypertrophy</subject><subject>Immunoenzyme Techniques</subject><subject>In Situ Hybridization</subject><subject>Luciferases - metabolism</subject><subject>Mice - embryology</subject><subject>Molecular Sequence Data</subject><subject>Muscle, Skeletal - cytology</subject><subject>Muscle, Skeletal - metabolism</subject><subject>Myocardium - cytology</subject><subject>Myocardium - metabolism</subject><subject>myosin heavy chain</subject><subject>Myosin Heavy Chains - genetics</subject><subject>Myosin Heavy Chains - metabolism</subject><subject>promoter analysis</subject><subject>Promoter Regions, Genetic - genetics</subject><subject>Rats</subject><subject>Real-Time Polymerase Chain Reaction</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>RNA, Messenger - genetics</subject><subject>Sequence Homology, Amino Acid</subject><subject>Sequence Homology, Nucleic Acid</subject><subject>smooth muscle</subject><subject>somites</subject><subject>Transcription Factors - genetics</subject><subject>Transcription Factors - metabolism</subject><subject>Transcription, Genetic</subject><subject>Transcriptional Activation</subject><subject>Xenopus laevis - embryology</subject><issn>1949-3584</issn><issn>1949-3592</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kU9v1DAQxSMEoqUg8QmQjxzY4n-J4wsS3dKCtC1CgOBmOfakMSRxsL3bRuLD46XbBQ5Iljye99PzjF5RPCX4mGBMX5rhmBJM5b3ikEguF6yU9P6-rvlB8SjGbxhXkmH2sDiglFMqaHVY_DyFDfR-GmBMukdwMwWI0fkR6dEio4N12qAU9BhNcFPKSsYCXK17vX0g36KLuRPNC6RR6lywaJh9dCPqQG9mZDqd63xSB2gDIUETdIKtGtLj4kGr-whPdvdR8fnszafl28Xq_fm75evVwpQkb2Bq2wrcNiXnDalFW-LaippVpSyl5cwyTAijxkpuBHBdcSYYa01rBcs9VrKj4tWt77RuBrAm7xp0r6bgBh1m5bVT_yqj69SV3yguGBdcZoPnO4Pgf6whJjW4aKDv9Qh-HRXBhBPKZI3_oCb4GAO0-28IVtuwlBnU77Ay-uzvsfbgXToZWNwC166H-b9GanlxZ7jjXUxws-d1-K4qwUSpvlyeq6-r0-XlhxOuPrJfyQ2u1A</recordid><startdate>201205</startdate><enddate>201205</enddate><creator>Warkman, Andrew S.</creator><creator>Whitman, Samantha A.</creator><creator>Miller, Melanie K.</creator><creator>Garriock, Robert J.</creator><creator>Schwach, Catherine M.</creator><creator>Gregorio, Carol C.</creator><creator>Krieg, Paul A.</creator><general>John Wiley & Sons, Inc</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>201205</creationdate><title>Developmental expression and cardiac transcriptional regulation of Myh7b, a third myosin heavy chain in the vertebrate heart</title><author>Warkman, Andrew S. ; Whitman, Samantha A. ; Miller, Melanie K. ; Garriock, Robert J. ; Schwach, Catherine M. ; Gregorio, Carol C. ; Krieg, Paul A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5149-c8df70fb544b187f508d78365959d43d301132cd94c7e4a643733fcfd73d94353</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Adult</topic><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Base Sequence</topic><topic>Binding Sites</topic><topic>Blotting, Western</topic><topic>cardiac development</topic><topic>Cardiomegaly - genetics</topic><topic>Cardiomegaly - metabolism</topic><topic>Cardiomegaly - pathology</topic><topic>Cell Nucleus - metabolism</topic><topic>Cells, Cultured</topic><topic>Chick Embryo</topic><topic>DNA-Binding Proteins - genetics</topic><topic>DNA-Binding Proteins - metabolism</topic><topic>Gene Expression Regulation, Developmental</topic><topic>Heart - embryology</topic><topic>Heart - physiology</topic><topic>Humans</topic><topic>hypertrophy</topic><topic>Immunoenzyme Techniques</topic><topic>In Situ Hybridization</topic><topic>Luciferases - metabolism</topic><topic>Mice - embryology</topic><topic>Molecular Sequence Data</topic><topic>Muscle, Skeletal - cytology</topic><topic>Muscle, Skeletal - metabolism</topic><topic>Myocardium - cytology</topic><topic>Myocardium - metabolism</topic><topic>myosin heavy chain</topic><topic>Myosin Heavy Chains - genetics</topic><topic>Myosin Heavy Chains - metabolism</topic><topic>promoter analysis</topic><topic>Promoter Regions, Genetic - genetics</topic><topic>Rats</topic><topic>Real-Time Polymerase Chain Reaction</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>RNA, Messenger - genetics</topic><topic>Sequence Homology, Amino Acid</topic><topic>Sequence Homology, Nucleic Acid</topic><topic>smooth muscle</topic><topic>somites</topic><topic>Transcription Factors - genetics</topic><topic>Transcription Factors - metabolism</topic><topic>Transcription, Genetic</topic><topic>Transcriptional Activation</topic><topic>Xenopus laevis - embryology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Warkman, Andrew S.</creatorcontrib><creatorcontrib>Whitman, Samantha A.</creatorcontrib><creatorcontrib>Miller, Melanie K.</creatorcontrib><creatorcontrib>Garriock, Robert J.</creatorcontrib><creatorcontrib>Schwach, Catherine M.</creatorcontrib><creatorcontrib>Gregorio, Carol C.</creatorcontrib><creatorcontrib>Krieg, Paul A.</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Cytoskeleton (Hoboken, N.J.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Warkman, Andrew S.</au><au>Whitman, Samantha A.</au><au>Miller, Melanie K.</au><au>Garriock, Robert J.</au><au>Schwach, Catherine M.</au><au>Gregorio, Carol C.</au><au>Krieg, Paul A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Developmental expression and cardiac transcriptional regulation of Myh7b, a third myosin heavy chain in the vertebrate heart</atitle><jtitle>Cytoskeleton (Hoboken, N.J.)</jtitle><addtitle>Cytoskeleton</addtitle><date>2012-05</date><risdate>2012</risdate><volume>69</volume><issue>5</issue><spage>324</spage><epage>335</epage><pages>324-335</pages><issn>1949-3584</issn><eissn>1949-3592</eissn><abstract>The mammalian heart expresses two myosin heavy chain (MYH) genes (Myh6 and Myh7), which are major components of the thick filaments of the sarcomere. We have determined that a third MYH, MYH7B, is also expressed in the myocardium. Developmental analysis shows Myh7b expression in cardiac and skeletal muscle of Xenopus, chick and mouse embryos, and in smooth muscle tissues during later stages of mouse embryogenesis. Myh7b is also expressed in the adult human heart. The promoter region of the Myh7b gene shows remarkable similarity between diverse species, suggesting that transcriptional control mechanisms have been conserved. Using luciferase reporter analysis in rat cardiomyocytes, it can be shown that MEF2, GATA, and E‐box regulatory elements are essential for efficient expression of the Myh7b gene. In addition two conserved elements that do not correspond to consensus binding sites for known transcription factors are also essential for full transcriptional activity of the Myh7b reporter. Finally, the Myh7b gene shows a transcriptional response similar to Myh6 in response to cardiac hypertrophy. © 2012 Wiley Periodicals, Inc</abstract><cop>Hoboken, USA</cop><pub>John Wiley & Sons, Inc</pub><pmid>22422726</pmid><doi>10.1002/cm.21029</doi><tpages>12</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Cytoskeleton (Hoboken, N.J.), 2012-05, Vol.69 (5), p.324-335 |
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| subjects | Adult Amino Acid Sequence Animals Base Sequence Binding Sites Blotting, Western cardiac development Cardiomegaly - genetics Cardiomegaly - metabolism Cardiomegaly - pathology Cell Nucleus - metabolism Cells, Cultured Chick Embryo DNA-Binding Proteins - genetics DNA-Binding Proteins - metabolism Gene Expression Regulation, Developmental Heart - embryology Heart - physiology Humans hypertrophy Immunoenzyme Techniques In Situ Hybridization Luciferases - metabolism Mice - embryology Molecular Sequence Data Muscle, Skeletal - cytology Muscle, Skeletal - metabolism Myocardium - cytology Myocardium - metabolism myosin heavy chain Myosin Heavy Chains - genetics Myosin Heavy Chains - metabolism promoter analysis Promoter Regions, Genetic - genetics Rats Real-Time Polymerase Chain Reaction Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger - genetics Sequence Homology, Amino Acid Sequence Homology, Nucleic Acid smooth muscle somites Transcription Factors - genetics Transcription Factors - metabolism Transcription, Genetic Transcriptional Activation Xenopus laevis - embryology |
| title | Developmental expression and cardiac transcriptional regulation of Myh7b, a third myosin heavy chain in the vertebrate heart |
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