Hydrogen peroxide-induced apoptosis in human gingival fibroblasts
In the process of bleaching vital, discolored teeth, low concentrations of hydrogen peroxide (H2O2) are effective alternatives to heat-activated 30% H2O2. However, interest has been expressed in the assessment of pathological effects of long-term exposure to bleaching agents such as irritation and u...
Gespeichert in:
| Veröffentlicht in: | International journal of clinical and experimental pathology 2015-01, Vol.8 (12), p.15563-15572 |
|---|---|
| Hauptverfasser: | , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 15572 |
|---|---|
| container_issue | 12 |
| container_start_page | 15563 |
| container_title | International journal of clinical and experimental pathology |
| container_volume | 8 |
| creator | Gutiérrez-Venegas, Gloria Guadarrama-Solís, Adriana Muñoz-Seca, Carmen Arreguín-Cano, Juan Antonio |
| description | In the process of bleaching vital, discolored teeth, low concentrations of hydrogen peroxide (H2O2) are effective alternatives to heat-activated 30% H2O2. However, interest has been expressed in the assessment of pathological effects of long-term exposure to bleaching agents such as irritation and ulceration of the gingival or other soft tissues. The aim of the present study was to determine the effect of hydrogen peroxide on apoptosis in human gingival fibroblasts (HGF). Cytochrome c, Bcl-2, Bax, Bid and caspase-3 protein expression were detected by Western blotting. HGF cell apoptosis induced by H2O2 was both dose and time dependent. The addition of H2O2 resulted in the release of cytochrome c to the cytosol, and an increase of Caspase-3 cleavage. Data suggest that oxidative stress-induced apoptosis in HGF is intrinsic pathway involved the release of apoptotic signal from mitochondria. |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_4730038</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1766264249</sourcerecordid><originalsourceid>FETCH-LOGICAL-p266t-6a90f846e6d3a4b28d4a2b6b3bc2d4dd9ad14b509b3c00f06034d23c34e500293</originalsourceid><addsrcrecordid>eNpVkE9LwzAYh4Mgbk6_gvTopfA2Sd-1F2EMdYOBFz2H_GsXaZOatMN9exWn6Ol3eOB54HdG5kXNMKdIyxm5TOkVAAvK4YLMKFYVr2g5J6vN0cTQWp8NNoZ3Z2zuvJm0NZkcwjCG5FLmfLafeumz1vnWHWSXNU7FoDqZxnRFzhvZJXt92gV5ebh_Xm_y3dPjdr3a5QNFHHOUNTQVR4uGSa5oZbikChVTmhpuTC1NwVUJtWIaoAEExg1lmnFbAtCaLcjdt3eYVG-Ntn6MshNDdL2MRxGkE_-Jd3vRhoPgSwbAqk_B7UkQw9tk0yh6l7TtOultmJIologUOeVfrZu_rd_Iz2_sAzMSakc</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1766264249</pqid></control><display><type>article</type><title>Hydrogen peroxide-induced apoptosis in human gingival fibroblasts</title><source>MEDLINE</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>PubMed Central</source><creator>Gutiérrez-Venegas, Gloria ; Guadarrama-Solís, Adriana ; Muñoz-Seca, Carmen ; Arreguín-Cano, Juan Antonio</creator><creatorcontrib>Gutiérrez-Venegas, Gloria ; Guadarrama-Solís, Adriana ; Muñoz-Seca, Carmen ; Arreguín-Cano, Juan Antonio</creatorcontrib><description>In the process of bleaching vital, discolored teeth, low concentrations of hydrogen peroxide (H2O2) are effective alternatives to heat-activated 30% H2O2. However, interest has been expressed in the assessment of pathological effects of long-term exposure to bleaching agents such as irritation and ulceration of the gingival or other soft tissues. The aim of the present study was to determine the effect of hydrogen peroxide on apoptosis in human gingival fibroblasts (HGF). Cytochrome c, Bcl-2, Bax, Bid and caspase-3 protein expression were detected by Western blotting. HGF cell apoptosis induced by H2O2 was both dose and time dependent. The addition of H2O2 resulted in the release of cytochrome c to the cytosol, and an increase of Caspase-3 cleavage. Data suggest that oxidative stress-induced apoptosis in HGF is intrinsic pathway involved the release of apoptotic signal from mitochondria.</description><identifier>EISSN: 1936-2625</identifier><identifier>PMID: 26884825</identifier><language>eng</language><publisher>United States: e-Century Publishing Corporation</publisher><subject>Adolescent ; Apoptosis - drug effects ; Apoptosis Regulatory Proteins - metabolism ; Cells, Cultured ; Dose-Response Relationship, Drug ; Fibroblasts - drug effects ; Fibroblasts - metabolism ; Fibroblasts - pathology ; Gingiva - drug effects ; Gingiva - metabolism ; Gingiva - pathology ; Humans ; Hydrogen Peroxide - toxicity ; Mitochondria - drug effects ; Mitochondria - metabolism ; Mitochondria - pathology ; Original ; Oxidative Stress - drug effects ; Time Factors ; Tooth Bleaching Agents - toxicity ; Young Adult</subject><ispartof>International journal of clinical and experimental pathology, 2015-01, Vol.8 (12), p.15563-15572</ispartof><rights>IJCEP Copyright © 2015 2015</rights><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4730038/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4730038/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26884825$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Gutiérrez-Venegas, Gloria</creatorcontrib><creatorcontrib>Guadarrama-Solís, Adriana</creatorcontrib><creatorcontrib>Muñoz-Seca, Carmen</creatorcontrib><creatorcontrib>Arreguín-Cano, Juan Antonio</creatorcontrib><title>Hydrogen peroxide-induced apoptosis in human gingival fibroblasts</title><title>International journal of clinical and experimental pathology</title><addtitle>Int J Clin Exp Pathol</addtitle><description>In the process of bleaching vital, discolored teeth, low concentrations of hydrogen peroxide (H2O2) are effective alternatives to heat-activated 30% H2O2. However, interest has been expressed in the assessment of pathological effects of long-term exposure to bleaching agents such as irritation and ulceration of the gingival or other soft tissues. The aim of the present study was to determine the effect of hydrogen peroxide on apoptosis in human gingival fibroblasts (HGF). Cytochrome c, Bcl-2, Bax, Bid and caspase-3 protein expression were detected by Western blotting. HGF cell apoptosis induced by H2O2 was both dose and time dependent. The addition of H2O2 resulted in the release of cytochrome c to the cytosol, and an increase of Caspase-3 cleavage. Data suggest that oxidative stress-induced apoptosis in HGF is intrinsic pathway involved the release of apoptotic signal from mitochondria.</description><subject>Adolescent</subject><subject>Apoptosis - drug effects</subject><subject>Apoptosis Regulatory Proteins - metabolism</subject><subject>Cells, Cultured</subject><subject>Dose-Response Relationship, Drug</subject><subject>Fibroblasts - drug effects</subject><subject>Fibroblasts - metabolism</subject><subject>Fibroblasts - pathology</subject><subject>Gingiva - drug effects</subject><subject>Gingiva - metabolism</subject><subject>Gingiva - pathology</subject><subject>Humans</subject><subject>Hydrogen Peroxide - toxicity</subject><subject>Mitochondria - drug effects</subject><subject>Mitochondria - metabolism</subject><subject>Mitochondria - pathology</subject><subject>Original</subject><subject>Oxidative Stress - drug effects</subject><subject>Time Factors</subject><subject>Tooth Bleaching Agents - toxicity</subject><subject>Young Adult</subject><issn>1936-2625</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVkE9LwzAYh4Mgbk6_gvTopfA2Sd-1F2EMdYOBFz2H_GsXaZOatMN9exWn6Ol3eOB54HdG5kXNMKdIyxm5TOkVAAvK4YLMKFYVr2g5J6vN0cTQWp8NNoZ3Z2zuvJm0NZkcwjCG5FLmfLafeumz1vnWHWSXNU7FoDqZxnRFzhvZJXt92gV5ebh_Xm_y3dPjdr3a5QNFHHOUNTQVR4uGSa5oZbikChVTmhpuTC1NwVUJtWIaoAEExg1lmnFbAtCaLcjdt3eYVG-Ntn6MshNDdL2MRxGkE_-Jd3vRhoPgSwbAqk_B7UkQw9tk0yh6l7TtOultmJIologUOeVfrZu_rd_Iz2_sAzMSakc</recordid><startdate>20150101</startdate><enddate>20150101</enddate><creator>Gutiérrez-Venegas, Gloria</creator><creator>Guadarrama-Solís, Adriana</creator><creator>Muñoz-Seca, Carmen</creator><creator>Arreguín-Cano, Juan Antonio</creator><general>e-Century Publishing Corporation</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20150101</creationdate><title>Hydrogen peroxide-induced apoptosis in human gingival fibroblasts</title><author>Gutiérrez-Venegas, Gloria ; Guadarrama-Solís, Adriana ; Muñoz-Seca, Carmen ; Arreguín-Cano, Juan Antonio</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p266t-6a90f846e6d3a4b28d4a2b6b3bc2d4dd9ad14b509b3c00f06034d23c34e500293</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Adolescent</topic><topic>Apoptosis - drug effects</topic><topic>Apoptosis Regulatory Proteins - metabolism</topic><topic>Cells, Cultured</topic><topic>Dose-Response Relationship, Drug</topic><topic>Fibroblasts - drug effects</topic><topic>Fibroblasts - metabolism</topic><topic>Fibroblasts - pathology</topic><topic>Gingiva - drug effects</topic><topic>Gingiva - metabolism</topic><topic>Gingiva - pathology</topic><topic>Humans</topic><topic>Hydrogen Peroxide - toxicity</topic><topic>Mitochondria - drug effects</topic><topic>Mitochondria - metabolism</topic><topic>Mitochondria - pathology</topic><topic>Original</topic><topic>Oxidative Stress - drug effects</topic><topic>Time Factors</topic><topic>Tooth Bleaching Agents - toxicity</topic><topic>Young Adult</topic><toplevel>online_resources</toplevel><creatorcontrib>Gutiérrez-Venegas, Gloria</creatorcontrib><creatorcontrib>Guadarrama-Solís, Adriana</creatorcontrib><creatorcontrib>Muñoz-Seca, Carmen</creatorcontrib><creatorcontrib>Arreguín-Cano, Juan Antonio</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>International journal of clinical and experimental pathology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gutiérrez-Venegas, Gloria</au><au>Guadarrama-Solís, Adriana</au><au>Muñoz-Seca, Carmen</au><au>Arreguín-Cano, Juan Antonio</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Hydrogen peroxide-induced apoptosis in human gingival fibroblasts</atitle><jtitle>International journal of clinical and experimental pathology</jtitle><addtitle>Int J Clin Exp Pathol</addtitle><date>2015-01-01</date><risdate>2015</risdate><volume>8</volume><issue>12</issue><spage>15563</spage><epage>15572</epage><pages>15563-15572</pages><eissn>1936-2625</eissn><abstract>In the process of bleaching vital, discolored teeth, low concentrations of hydrogen peroxide (H2O2) are effective alternatives to heat-activated 30% H2O2. However, interest has been expressed in the assessment of pathological effects of long-term exposure to bleaching agents such as irritation and ulceration of the gingival or other soft tissues. The aim of the present study was to determine the effect of hydrogen peroxide on apoptosis in human gingival fibroblasts (HGF). Cytochrome c, Bcl-2, Bax, Bid and caspase-3 protein expression were detected by Western blotting. HGF cell apoptosis induced by H2O2 was both dose and time dependent. The addition of H2O2 resulted in the release of cytochrome c to the cytosol, and an increase of Caspase-3 cleavage. Data suggest that oxidative stress-induced apoptosis in HGF is intrinsic pathway involved the release of apoptotic signal from mitochondria.</abstract><cop>United States</cop><pub>e-Century Publishing Corporation</pub><pmid>26884825</pmid><tpages>10</tpages></addata></record> |
| fulltext | fulltext |
| identifier | EISSN: 1936-2625 |
| ispartof | International journal of clinical and experimental pathology, 2015-01, Vol.8 (12), p.15563-15572 |
| issn | 1936-2625 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_4730038 |
| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central |
| subjects | Adolescent Apoptosis - drug effects Apoptosis Regulatory Proteins - metabolism Cells, Cultured Dose-Response Relationship, Drug Fibroblasts - drug effects Fibroblasts - metabolism Fibroblasts - pathology Gingiva - drug effects Gingiva - metabolism Gingiva - pathology Humans Hydrogen Peroxide - toxicity Mitochondria - drug effects Mitochondria - metabolism Mitochondria - pathology Original Oxidative Stress - drug effects Time Factors Tooth Bleaching Agents - toxicity Young Adult |
| title | Hydrogen peroxide-induced apoptosis in human gingival fibroblasts |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-19T06%3A39%3A17IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Hydrogen%20peroxide-induced%20apoptosis%20in%20human%20gingival%20fibroblasts&rft.jtitle=International%20journal%20of%20clinical%20and%20experimental%20pathology&rft.au=Guti%C3%A9rrez-Venegas,%20Gloria&rft.date=2015-01-01&rft.volume=8&rft.issue=12&rft.spage=15563&rft.epage=15572&rft.pages=15563-15572&rft.eissn=1936-2625&rft_id=info:doi/&rft_dat=%3Cproquest_pubme%3E1766264249%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1766264249&rft_id=info:pmid/26884825&rfr_iscdi=true |