Effect of local CTLA4Ig gene transfection on acute rejection of small bowel allografts in rats

AIM:To evaluate the local expression of CTLA4Ig gene in small bowels and its effect on preventing acute rejection of the small bowel allografts.METHODS:Groups of Wistar rats underwent heterotopic small bowel transplantation from SD rats.The recipients were randomly divided into experimental group (a...

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Veröffentlicht in:World journal of gastroenterology : WJG 2004-03, Vol.10 (6), p.885-888
Hauptverfasser: Wang, Yi-Fang, Xu, Ai-Gang, Hua, Yi-Bing, Wu, Wen-Xi
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container_title World journal of gastroenterology : WJG
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creator Wang, Yi-Fang
Xu, Ai-Gang
Hua, Yi-Bing
Wu, Wen-Xi
description AIM:To evaluate the local expression of CTLA4Ig gene in small bowels and its effect on preventing acute rejection of the small bowel allografts.METHODS:Groups of Wistar rats underwent heterotopic small bowel transplantation from SD rats.The recipients were randomly divided into experimental group (allografts were transfected with CTLA4Ig gene) and control group (non CTLA4Ig gene transfected).In the experimental group, the donor small bowels were perfused ex vivo with CTLA4Ig cDNA packaged with lipofectin vector via intra-superior mesenteric artery before transplantation, and the CTLA4Ig expression in the small bowel grafts post-transplantation was assessed by immunohistology. On d 3, 7 and 10 post-transplantation,the allografts in each group were harvested for the examination of histology and detection of apoptosis.RESULTS:Small bowel allografts treated with CTLA4Ig cDNA showed abundant CTLA4Ig expression after transplantation.Acute rejection of grade I on d 7 and grade Ⅱ on d 10 after transplantation was noticed in the control allografts,and a dramatically increased number of apoptotic enterocytes in parallel to the progressive rejection could be recognized.In contrast,the allografts treated with CTLA4Ig cDNA showed nonspecific histological changes and only a few apoptotic enterocytes were found after transplantation.CONCLUSION: Local CTLA4Ig gene transfection of small bowel allograft is feasible, and the local CTLA4Ig expression in the allograft can prevent acute rejection after transplantation.
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On d 3, 7 and 10 post-transplantation,the allografts in each group were harvested for the examination of histology and detection of apoptosis.RESULTS:Small bowel allografts treated with CTLA4Ig cDNA showed abundant CTLA4Ig expression after transplantation.Acute rejection of grade I on d 7 and grade Ⅱ on d 10 after transplantation was noticed in the control allografts,and a dramatically increased number of apoptotic enterocytes in parallel to the progressive rejection could be recognized.In contrast,the allografts treated with CTLA4Ig cDNA showed nonspecific histological changes and only a few apoptotic enterocytes were found after transplantation.CONCLUSION: Local CTLA4Ig gene transfection of small bowel allograft is feasible, and the local CTLA4Ig expression in the allograft can prevent acute rejection after transplantation.</description><identifier>ISSN: 1007-9327</identifier><identifier>EISSN: 2219-2840</identifier><identifier>DOI: 10.3748/wjg.v10.i6.885</identifier><identifier>PMID: 15040038</identifier><language>eng</language><publisher>United States: Departnent of Gastrointestinal Surgery, the First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, Jiangsu Province, China</publisher><subject>Abatacept ; Acute Disease ; Animals ; Basic Research ; CTLA4Ig基因 ; Graft Rejection - prevention &amp; control ; Immunoconjugates - genetics ; Intestine, Small - transplantation ; Male ; Rats ; Rats, Sprague-Dawley ; Rats, Wistar ; Transfection ; Transplantation, Heterotopic ; Transplantation, Homologous ; 动物实验 ; 同种异体小肠移植 ; 基因转染 ; 急性排斥反应</subject><ispartof>World journal of gastroenterology : WJG, 2004-03, Vol.10 (6), p.885-888</ispartof><rights>Copyright © Wanfang Data Co. Ltd. All Rights Reserved.</rights><rights>The Author(s) 2004. Published by Baishideng Publishing Group Inc. All rights reserved. 2004</rights><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c373t-d045823efadc26b5baee04ba74db3805be15cf16ad2e055806b00178cb6e2a673</citedby><cites>FETCH-LOGICAL-c373t-d045823efadc26b5baee04ba74db3805be15cf16ad2e055806b00178cb6e2a673</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://image.cqvip.com/vip1000/qk/84123X/84123X.jpg</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4727007/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4727007/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15040038$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wang, Yi-Fang</creatorcontrib><creatorcontrib>Xu, Ai-Gang</creatorcontrib><creatorcontrib>Hua, Yi-Bing</creatorcontrib><creatorcontrib>Wu, Wen-Xi</creatorcontrib><title>Effect of local CTLA4Ig gene transfection on acute rejection of small bowel allografts in rats</title><title>World journal of gastroenterology : WJG</title><addtitle>World Journal of Gastroenterology</addtitle><description>AIM:To evaluate the local expression of CTLA4Ig gene in small bowels and its effect on preventing acute rejection of the small bowel allografts.METHODS:Groups of Wistar rats underwent heterotopic small bowel transplantation from SD rats.The recipients were randomly divided into experimental group (allografts were transfected with CTLA4Ig gene) and control group (non CTLA4Ig gene transfected).In the experimental group, the donor small bowels were perfused ex vivo with CTLA4Ig cDNA packaged with lipofectin vector via intra-superior mesenteric artery before transplantation, and the CTLA4Ig expression in the small bowel grafts post-transplantation was assessed by immunohistology. On d 3, 7 and 10 post-transplantation,the allografts in each group were harvested for the examination of histology and detection of apoptosis.RESULTS:Small bowel allografts treated with CTLA4Ig cDNA showed abundant CTLA4Ig expression after transplantation.Acute rejection of grade I on d 7 and grade Ⅱ on d 10 after transplantation was noticed in the control allografts,and a dramatically increased number of apoptotic enterocytes in parallel to the progressive rejection could be recognized.In contrast,the allografts treated with CTLA4Ig cDNA showed nonspecific histological changes and only a few apoptotic enterocytes were found after transplantation.CONCLUSION: Local CTLA4Ig gene transfection of small bowel allograft is feasible, and the local CTLA4Ig expression in the allograft can prevent acute rejection after transplantation.</description><subject>Abatacept</subject><subject>Acute Disease</subject><subject>Animals</subject><subject>Basic Research</subject><subject>CTLA4Ig基因</subject><subject>Graft Rejection - prevention &amp; control</subject><subject>Immunoconjugates - genetics</subject><subject>Intestine, Small - transplantation</subject><subject>Male</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Rats, Wistar</subject><subject>Transfection</subject><subject>Transplantation, Heterotopic</subject><subject>Transplantation, Homologous</subject><subject>动物实验</subject><subject>同种异体小肠移植</subject><subject>基因转染</subject><subject>急性排斥反应</subject><issn>1007-9327</issn><issn>2219-2840</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVUV1vFCEUJUZj1-qrjwaTxrdZ-RiGmReTZlNtk018qa8SYC5TVhZamO3Gfy_rbv1ISIB7D-cezkHoLSVLLtv-434zLR_rxXfLvhfP0IIxOjSsb8lztKCEyGbgTJ6hV6VsCGGcC_YSnVFBWkJ4v0Dfr5wDO-PkcEhWB7y6XV-2NxOeIAKes47l0Pcp4rq03c2AM2yeSg6XrQ4Bm7SHgOspTVm7uWAfcdZzeY1eOB0KvDnt5-jb56vb1XWz_vrlZnW5biyXfG5G0oqecXB6tKwzwmgA0hot29HwnggDVFhHOz0yIEL0pDOEUNlb0wHTneTn6NOR935ntjBaiFV6UPfZb3X-qZL26v9O9HdqSo-qlUxWlyrBxZFgr6PTcVKbtMuxSlbVYUaqXR1hbYV9OM3J6WEHZVZbXyyEoCOkXVGSyo7R38DlEWhzKiWD-6OFEnVI7sCranLKd6omVx-8-_cHf-GnqCrg_YnxLsXpwVeNRtsfzgdQg-BkGBj_BWjMoXc</recordid><startdate>20040315</startdate><enddate>20040315</enddate><creator>Wang, Yi-Fang</creator><creator>Xu, Ai-Gang</creator><creator>Hua, Yi-Bing</creator><creator>Wu, Wen-Xi</creator><general>Departnent of Gastrointestinal Surgery, the First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, Jiangsu Province, China</general><general>Baishideng Publishing Group Inc</general><scope>2RA</scope><scope>92L</scope><scope>CQIGP</scope><scope>W91</scope><scope>~WA</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>2B.</scope><scope>4A8</scope><scope>92I</scope><scope>93N</scope><scope>PSX</scope><scope>TCJ</scope><scope>5PM</scope></search><sort><creationdate>20040315</creationdate><title>Effect of local CTLA4Ig gene transfection on acute rejection of small bowel allografts in rats</title><author>Wang, Yi-Fang ; Xu, Ai-Gang ; Hua, Yi-Bing ; Wu, Wen-Xi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c373t-d045823efadc26b5baee04ba74db3805be15cf16ad2e055806b00178cb6e2a673</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Abatacept</topic><topic>Acute Disease</topic><topic>Animals</topic><topic>Basic Research</topic><topic>CTLA4Ig基因</topic><topic>Graft Rejection - prevention &amp; control</topic><topic>Immunoconjugates - genetics</topic><topic>Intestine, Small - transplantation</topic><topic>Male</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Rats, Wistar</topic><topic>Transfection</topic><topic>Transplantation, Heterotopic</topic><topic>Transplantation, Homologous</topic><topic>动物实验</topic><topic>同种异体小肠移植</topic><topic>基因转染</topic><topic>急性排斥反应</topic><toplevel>online_resources</toplevel><creatorcontrib>Wang, Yi-Fang</creatorcontrib><creatorcontrib>Xu, Ai-Gang</creatorcontrib><creatorcontrib>Hua, Yi-Bing</creatorcontrib><creatorcontrib>Wu, Wen-Xi</creatorcontrib><collection>中文科技期刊数据库</collection><collection>中文科技期刊数据库-CALIS站点</collection><collection>中文科技期刊数据库-7.0平台</collection><collection>中文科技期刊数据库-医药卫生</collection><collection>中文科技期刊数据库- 镜像站点</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Wanfang Data Journals - Hong Kong</collection><collection>WANFANG Data Centre</collection><collection>Wanfang Data Journals</collection><collection>万方数据期刊 - 香港版</collection><collection>China Online Journals (COJ)</collection><collection>China Online Journals (COJ)</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>World journal of gastroenterology : WJG</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wang, Yi-Fang</au><au>Xu, Ai-Gang</au><au>Hua, Yi-Bing</au><au>Wu, Wen-Xi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of local CTLA4Ig gene transfection on acute rejection of small bowel allografts in rats</atitle><jtitle>World journal of gastroenterology : WJG</jtitle><addtitle>World Journal of Gastroenterology</addtitle><date>2004-03-15</date><risdate>2004</risdate><volume>10</volume><issue>6</issue><spage>885</spage><epage>888</epage><pages>885-888</pages><issn>1007-9327</issn><eissn>2219-2840</eissn><abstract>AIM:To evaluate the local expression of CTLA4Ig gene in small bowels and its effect on preventing acute rejection of the small bowel allografts.METHODS:Groups of Wistar rats underwent heterotopic small bowel transplantation from SD rats.The recipients were randomly divided into experimental group (allografts were transfected with CTLA4Ig gene) and control group (non CTLA4Ig gene transfected).In the experimental group, the donor small bowels were perfused ex vivo with CTLA4Ig cDNA packaged with lipofectin vector via intra-superior mesenteric artery before transplantation, and the CTLA4Ig expression in the small bowel grafts post-transplantation was assessed by immunohistology. On d 3, 7 and 10 post-transplantation,the allografts in each group were harvested for the examination of histology and detection of apoptosis.RESULTS:Small bowel allografts treated with CTLA4Ig cDNA showed abundant CTLA4Ig expression after transplantation.Acute rejection of grade I on d 7 and grade Ⅱ on d 10 after transplantation was noticed in the control allografts,and a dramatically increased number of apoptotic enterocytes in parallel to the progressive rejection could be recognized.In contrast,the allografts treated with CTLA4Ig cDNA showed nonspecific histological changes and only a few apoptotic enterocytes were found after transplantation.CONCLUSION: Local CTLA4Ig gene transfection of small bowel allograft is feasible, and the local CTLA4Ig expression in the allograft can prevent acute rejection after transplantation.</abstract><cop>United States</cop><pub>Departnent of Gastrointestinal Surgery, the First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, Jiangsu Province, China</pub><pmid>15040038</pmid><doi>10.3748/wjg.v10.i6.885</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record>
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subjects Abatacept
Acute Disease
Animals
Basic Research
CTLA4Ig基因
Graft Rejection - prevention & control
Immunoconjugates - genetics
Intestine, Small - transplantation
Male
Rats
Rats, Sprague-Dawley
Rats, Wistar
Transfection
Transplantation, Heterotopic
Transplantation, Homologous
动物实验
同种异体小肠移植
基因转染
急性排斥反应
title Effect of local CTLA4Ig gene transfection on acute rejection of small bowel allografts in rats
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