Downregulation of retinoic acid receptor-β2expression is linked to aberrant methylation in esophageal squamous cell carcinoma cell lines
AIM:To study the role of hypermethylation in the loss of retinoic acid receptor β2(RARβ2) in esophageal squamous cell carcinoma (ESCC).METHODS:The role of hypermethylation in RARβ2 gene silencing in 6 ESCC cell lines was determined by methylation-specific PCR (MSP),and its methylation status was com...
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| Veröffentlicht in: | World journal of gastroenterology : WJG 2004-03, Vol.10 (6), p.771-775 |
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| container_title | World journal of gastroenterology : WJG |
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| creator | Liu, Zhong-Min Ding, Fang Guo, Ming-Zhou Zhang, Li-Yong Wu, Min Liu, Zhi-Hua |
| description | AIM:To study the role of hypermethylation in the loss of retinoic acid receptor β2(RARβ2) in esophageal squamous cell carcinoma (ESCC).METHODS:The role of hypermethylation in RARβ2 gene silencing in 6 ESCC cell lines was determined by methylation-specific PCR (MSP),and its methylation status was compared with RARβ2 mRNA expression by RT-PCR.The MSP results were confirmed by bisulfite sequencing of RARβ2promoter regions.RESULTS:Methylation was detected in 4 of the 6 cell lines,and the expression of RARβ2 was markedly downregulated in 3 of the 4 methylated cell lines. The expression of RARβ2 was restored in one RARβ2-downregulated cell line with the partial demethylation of promoter region of RARβ2 after 5-aza-2′-deoxycytidine (5-aza-dc) treatment.CONCLUSION:The methylation of the 5′ region may play an important role in the downregulation of RARβ2 in some ESCC cell lines, suggesting that multiple mechanisms contribute to the loss of RARβ2expression in ESCC cell lines.This study may have clinical applications for treatment and prevention of ESCC. |
| doi_str_mv | 10.3748/wjg.v10.i6.771 |
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The expression of RARβ2 was restored in one RARβ2-downregulated cell line with the partial demethylation of promoter region of RARβ2 after 5-aza-2′-deoxycytidine (5-aza-dc) treatment.CONCLUSION:The methylation of the 5′ region may play an important role in the downregulation of RARβ2 in some ESCC cell lines, suggesting that multiple mechanisms contribute to the loss of RARβ2expression in ESCC cell lines.This study may have clinical applications for treatment and prevention of ESCC.</description><identifier>ISSN: 1007-9327</identifier><identifier>EISSN: 2219-2840</identifier><identifier>DOI: 10.3748/wjg.v10.i6.771</identifier><identifier>PMID: 15040015</identifier><language>eng</language><publisher>National Laboratory of Molecular Oncology, Cancer Institute, Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing, China%Division of Tumor Biology, Department of Oncology, The Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins, Baltimore, MD, USA</publisher><subject>Esophageal Cancer ; 甲基化 ; 细胞培养 ; 维甲酸受体β2 ; 肿瘤生物学 ; 肿瘤细胞 ; 食管鳞状细胞癌</subject><ispartof>World journal of gastroenterology : WJG, 2004-03, Vol.10 (6), p.771-775</ispartof><rights>Copyright © Wanfang Data Co. Ltd. All Rights Reserved.</rights><rights>The Author(s) 2004. Published by Baishideng Publishing Group Inc. All rights reserved. 2004</rights><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://image.cqvip.com/vip1000/qk/84123X/84123X.jpg</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4726992/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4726992/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,723,776,780,881,27901,27902,53766,53768</link.rule.ids></links><search><creatorcontrib>Liu, Zhong-Min</creatorcontrib><creatorcontrib>Ding, Fang</creatorcontrib><creatorcontrib>Guo, Ming-Zhou</creatorcontrib><creatorcontrib>Zhang, Li-Yong</creatorcontrib><creatorcontrib>Wu, Min</creatorcontrib><creatorcontrib>Liu, Zhi-Hua</creatorcontrib><title>Downregulation of retinoic acid receptor-β2expression is linked to aberrant methylation in esophageal squamous cell carcinoma cell lines</title><title>World journal of gastroenterology : WJG</title><addtitle>World Journal of Gastroenterology</addtitle><description>AIM:To study the role of hypermethylation in the loss of retinoic acid receptor β2(RARβ2) in esophageal squamous cell carcinoma (ESCC).METHODS:The role of hypermethylation in RARβ2 gene silencing in 6 ESCC cell lines was determined by methylation-specific PCR (MSP),and its methylation status was compared with RARβ2 mRNA expression by RT-PCR.The MSP results were confirmed by bisulfite sequencing of RARβ2promoter regions.RESULTS:Methylation was detected in 4 of the 6 cell lines,and the expression of RARβ2 was markedly downregulated in 3 of the 4 methylated cell lines. The expression of RARβ2 was restored in one RARβ2-downregulated cell line with the partial demethylation of promoter region of RARβ2 after 5-aza-2′-deoxycytidine (5-aza-dc) treatment.CONCLUSION:The methylation of the 5′ region may play an important role in the downregulation of RARβ2 in some ESCC cell lines, suggesting that multiple mechanisms contribute to the loss of RARβ2expression in ESCC cell lines.This study may have clinical applications for treatment and prevention of ESCC.</description><subject>Esophageal Cancer</subject><subject>甲基化</subject><subject>细胞培养</subject><subject>维甲酸受体β2</subject><subject>肿瘤生物学</subject><subject>肿瘤细胞</subject><subject>食管鳞状细胞癌</subject><issn>1007-9327</issn><issn>2219-2840</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><recordid>eNpVkU2O1DAQhS0EYpqBLWsjsU1jl52_DRIafqWR2MA6qtiVtHsSO2Mn08wRuA4H4Ux41LNhVXqqp6_qVTH2Woq9qnXz7nQc93dZuGpf1_IJ2wHItoBGi6dsJ4Woi1ZBfcFepHQUApQq4Tm7kKXQQshyx35_DCcfadwmXF3wPAw80up8cIajcTYrQ8saYvH3D9CvJVJKDz6X-OT8DVm-Bo49xYh-5TOth_tHkvOcUlgOOBJOPN1uOIctcUPTxA1Gk2fMeJaZROklezbglOjVY71kPz9_-nH1tbj-_uXb1YfrwoBsZFGB7ZW2RpPuUdimBKmxaawZQEMrH5Ib24ph6KWtcMh5EUGAUL2iUqJRl-z9mbts_UzWkF8jTt0S3Yzxvgvouv873h26Mdx1uoaqbSED3p4BJ_QD-rE7hi36vHKXnwEiX7bKt822N2ebOQQ_3rps7NHcDG6iri2VaGtQ_wDpo40a</recordid><startdate>20040315</startdate><enddate>20040315</enddate><creator>Liu, Zhong-Min</creator><creator>Ding, Fang</creator><creator>Guo, Ming-Zhou</creator><creator>Zhang, Li-Yong</creator><creator>Wu, Min</creator><creator>Liu, Zhi-Hua</creator><general>National Laboratory of Molecular Oncology, Cancer Institute, Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing, China%Division of Tumor Biology, Department of Oncology, The Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins, Baltimore, MD, USA</general><general>Baishideng Publishing Group Inc</general><scope>2RA</scope><scope>92L</scope><scope>CQIGP</scope><scope>W91</scope><scope>~WA</scope><scope>2B.</scope><scope>4A8</scope><scope>92I</scope><scope>93N</scope><scope>PSX</scope><scope>TCJ</scope><scope>5PM</scope></search><sort><creationdate>20040315</creationdate><title>Downregulation of retinoic acid receptor-β2expression is linked to aberrant methylation in esophageal squamous cell carcinoma cell lines</title><author>Liu, Zhong-Min ; Ding, Fang ; Guo, Ming-Zhou ; Zhang, Li-Yong ; Wu, Min ; Liu, Zhi-Hua</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c2181-62db34dc4e4ba0d85214a88dcf242912840cd90ffb1d6af352aa20203b3e51ac3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Esophageal Cancer</topic><topic>甲基化</topic><topic>细胞培养</topic><topic>维甲酸受体β2</topic><topic>肿瘤生物学</topic><topic>肿瘤细胞</topic><topic>食管鳞状细胞癌</topic><toplevel>online_resources</toplevel><creatorcontrib>Liu, Zhong-Min</creatorcontrib><creatorcontrib>Ding, Fang</creatorcontrib><creatorcontrib>Guo, Ming-Zhou</creatorcontrib><creatorcontrib>Zhang, Li-Yong</creatorcontrib><creatorcontrib>Wu, Min</creatorcontrib><creatorcontrib>Liu, Zhi-Hua</creatorcontrib><collection>维普_期刊</collection><collection>中文科技期刊数据库-CALIS站点</collection><collection>维普中文期刊数据库</collection><collection>中文科技期刊数据库-医药卫生</collection><collection>中文科技期刊数据库- 镜像站点</collection><collection>Wanfang Data Journals - Hong Kong</collection><collection>WANFANG Data Centre</collection><collection>Wanfang Data Journals</collection><collection>万方数据期刊 - 香港版</collection><collection>China Online Journals (COJ)</collection><collection>China Online Journals (COJ)</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>World journal of gastroenterology : WJG</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Liu, Zhong-Min</au><au>Ding, Fang</au><au>Guo, Ming-Zhou</au><au>Zhang, Li-Yong</au><au>Wu, Min</au><au>Liu, Zhi-Hua</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Downregulation of retinoic acid receptor-β2expression is linked to aberrant methylation in esophageal squamous cell carcinoma cell lines</atitle><jtitle>World journal of gastroenterology : WJG</jtitle><addtitle>World Journal of Gastroenterology</addtitle><date>2004-03-15</date><risdate>2004</risdate><volume>10</volume><issue>6</issue><spage>771</spage><epage>775</epage><pages>771-775</pages><issn>1007-9327</issn><eissn>2219-2840</eissn><abstract>AIM:To study the role of hypermethylation in the loss of retinoic acid receptor β2(RARβ2) in esophageal squamous cell carcinoma (ESCC).METHODS:The role of hypermethylation in RARβ2 gene silencing in 6 ESCC cell lines was determined by methylation-specific PCR (MSP),and its methylation status was compared with RARβ2 mRNA expression by RT-PCR.The MSP results were confirmed by bisulfite sequencing of RARβ2promoter regions.RESULTS:Methylation was detected in 4 of the 6 cell lines,and the expression of RARβ2 was markedly downregulated in 3 of the 4 methylated cell lines. The expression of RARβ2 was restored in one RARβ2-downregulated cell line with the partial demethylation of promoter region of RARβ2 after 5-aza-2′-deoxycytidine (5-aza-dc) treatment.CONCLUSION:The methylation of the 5′ region may play an important role in the downregulation of RARβ2 in some ESCC cell lines, suggesting that multiple mechanisms contribute to the loss of RARβ2expression in ESCC cell lines.This study may have clinical applications for treatment and prevention of ESCC.</abstract><pub>National Laboratory of Molecular Oncology, Cancer Institute, Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing, China%Division of Tumor Biology, Department of Oncology, The Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins, Baltimore, MD, USA</pub><pmid>15040015</pmid><doi>10.3748/wjg.v10.i6.771</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| source | PubMed Central (PMC); Alma/SFX Local Collection |
| subjects | Esophageal Cancer 甲基化 细胞培养 维甲酸受体β2 肿瘤生物学 肿瘤细胞 食管鳞状细胞癌 |
| title | Downregulation of retinoic acid receptor-β2expression is linked to aberrant methylation in esophageal squamous cell carcinoma cell lines |
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