CRMAGE: CRISPR Optimized MAGE Recombineering

A bottleneck in metabolic engineering and systems biology approaches is the lack of efficient genome engineering technologies. Here, we combine CRISPR/Cas9 and λ Red recombineering based MAGE technology (CRMAGE) to create a highly efficient and fast method for genome engineering of Escherichia coli...

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Veröffentlicht in:	Scientific reports 2016-01, Vol.6 (1), p.19452, Article 19452
Hauptverfasser:	Ronda, Carlotta, Pedersen, Lasse Ebdrup, Sommer, Morten O. A., Nielsen, Alex Toftgaard
Format:	Artikel
Sprache:	eng
Schlagworte:	42/35 42/41 42/44 631/1647/1511 631/1647/2234 631/326/1320 Automation Cell Death Cloning Codons CRISPR CRISPR-Cas Systems - genetics E coli Engineering Genetic Engineering - methods Genome editing Genomes gRNA Humanities and Social Sciences Metabolic engineering multidisciplinary Mutagenesis, Insertional Mutation - genetics MutS DNA Mismatch-Binding Protein - metabolism Nucleotide Motifs - genetics Oligonucleotides - metabolism Plasmids Plasmids - genetics Protein Biosynthesis Protein synthesis Rec A Recombinases - metabolism RNA, Guide, CRISPR-Cas Systems - genetics Science
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