Development of a luminescent G-quadruplex-selective iridium(III) complex for the label-free detection of adenosine
A panel of six luminescent iridium(III) complexes were synthesized and evaluated for their ability to act as G-quadruplex-selective probes. The novel iridium(III) complex 1 was found to be highly selective for G-quadruplex DNA and was employed for the construction of a label-free G-quadruplex-based...
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| Veröffentlicht in: | Scientific reports 2016-01, Vol.6 (1), p.19368-19368, Article 19368 |
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| creator | Lu, Lihua Zhong, Hai-Jing He, Bingyong Leung, Chung-Hang Ma, Dik-Lung |
| description | A panel of six luminescent iridium(III) complexes were synthesized and evaluated for their ability to act as G-quadruplex-selective probes. The novel iridium(III) complex
1
was found to be highly selective for G-quadruplex DNA and was employed for the construction of a label-free G-quadruplex-based adenosine detection assay in aqueous solution. Two different detection strategies were investigated for adenosine detection and the results showed that initial addition of adenosine to the adenosine aptamer gave superior results. The assay exhibited a linear response for adenosine in the concentration range of 5 to 120 μM (R
2
= 0.992) and the limit of detection for adenosine was 5 μM. Moreover, this assay was highly selective for adenosine over other nucleosides and exhibited potential use for biological sample analysis. |
| doi_str_mv | 10.1038/srep19368 |
| format | Article |
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1
was found to be highly selective for G-quadruplex DNA and was employed for the construction of a label-free G-quadruplex-based adenosine detection assay in aqueous solution. Two different detection strategies were investigated for adenosine detection and the results showed that initial addition of adenosine to the adenosine aptamer gave superior results. The assay exhibited a linear response for adenosine in the concentration range of 5 to 120 μM (R
2
= 0.992) and the limit of detection for adenosine was 5 μM. Moreover, this assay was highly selective for adenosine over other nucleosides and exhibited potential use for biological sample analysis.</description><identifier>ISSN: 2045-2322</identifier><identifier>EISSN: 2045-2322</identifier><identifier>DOI: 10.1038/srep19368</identifier><identifier>PMID: 26778273</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>639/638/263/49 ; 639/638/263/914 ; 9/10 ; Adenosine ; Aptamers ; Biosensing Techniques ; Chromatography ; Coordination Complexes - chemistry ; Deoxyribonucleic acid ; DNA ; DNA probes ; G-Quadruplexes ; Humanities and Social Sciences ; Iridium ; Laboratories ; Ligands ; Luminescence ; Luminescent Measurements ; Molecular Structure ; multidisciplinary ; Nervous system ; Nucleosides ; Science</subject><ispartof>Scientific reports, 2016-01, Vol.6 (1), p.19368-19368, Article 19368</ispartof><rights>The Author(s) 2016</rights><rights>Copyright Nature Publishing Group Jan 2016</rights><rights>Copyright © 2016, Macmillan Publishers Limited 2016 Macmillan Publishers Limited</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c438t-64dfd0f463cb6ca1edf888120e6d8cc8667620bcf2742cd442565ed8a35809c73</citedby><cites>FETCH-LOGICAL-c438t-64dfd0f463cb6ca1edf888120e6d8cc8667620bcf2742cd442565ed8a35809c73</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4726048/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4726048/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,27924,27925,41120,42189,51576,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26778273$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lu, Lihua</creatorcontrib><creatorcontrib>Zhong, Hai-Jing</creatorcontrib><creatorcontrib>He, Bingyong</creatorcontrib><creatorcontrib>Leung, Chung-Hang</creatorcontrib><creatorcontrib>Ma, Dik-Lung</creatorcontrib><title>Development of a luminescent G-quadruplex-selective iridium(III) complex for the label-free detection of adenosine</title><title>Scientific reports</title><addtitle>Sci Rep</addtitle><addtitle>Sci Rep</addtitle><description>A panel of six luminescent iridium(III) complexes were synthesized and evaluated for their ability to act as G-quadruplex-selective probes. The novel iridium(III) complex
1
was found to be highly selective for G-quadruplex DNA and was employed for the construction of a label-free G-quadruplex-based adenosine detection assay in aqueous solution. Two different detection strategies were investigated for adenosine detection and the results showed that initial addition of adenosine to the adenosine aptamer gave superior results. The assay exhibited a linear response for adenosine in the concentration range of 5 to 120 μM (R
2
= 0.992) and the limit of detection for adenosine was 5 μM. Moreover, this assay was highly selective for adenosine over other nucleosides and exhibited potential use for biological sample analysis.</description><subject>639/638/263/49</subject><subject>639/638/263/914</subject><subject>9/10</subject><subject>Adenosine</subject><subject>Aptamers</subject><subject>Biosensing Techniques</subject><subject>Chromatography</subject><subject>Coordination Complexes - chemistry</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>DNA probes</subject><subject>G-Quadruplexes</subject><subject>Humanities and Social Sciences</subject><subject>Iridium</subject><subject>Laboratories</subject><subject>Ligands</subject><subject>Luminescence</subject><subject>Luminescent Measurements</subject><subject>Molecular Structure</subject><subject>multidisciplinary</subject><subject>Nervous system</subject><subject>Nucleosides</subject><subject>Science</subject><issn>2045-2322</issn><issn>2045-2322</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>C6C</sourceid><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNplkUFPHSEUhUljU4110T9gSLpRk2mBYRhmY9JYtS8x6aZdEx5cFMMMI8y8tP--TJ--PFs2EM7HufdyEPpAySdKavk5JxhpVwv5Bh0xwpuK1Ywd7J0P0UnOj6SshnWcdu_QIRNtK1lbH6H0FTYQ4tjDMOHosMZh7v0A2SwXt9XTrG2axwC_qgwBzOQ3gH3y1s_92Wq1Oscm9ouMXUx4egAc9BpC5RIAtjAtL-Lw19nCEHOxfo_eOh0ynDzvx-jnzfWPq2_V3ffb1dWXu8rwWk6V4NZZ4riozVoYTcE6KSVlBISVxkghWsHI2jjWcmYs56wRDVip60aSzrT1Mbrc-o7zuge7DJR0UGPyvU6_VdRevVYG_6Du40bxlgnCZTE4ezZI8WmGPKnel38JQQ8Q56xoK4iUhLKmoB__QR_jnIYynqKy6whnHSGFOt9SJsVccnO7ZihRS5hqF2ZhT_e735Ev0RXgYgvkIg33kPZK_uf2B0stqk4</recordid><startdate>20160118</startdate><enddate>20160118</enddate><creator>Lu, Lihua</creator><creator>Zhong, Hai-Jing</creator><creator>He, Bingyong</creator><creator>Leung, Chung-Hang</creator><creator>Ma, Dik-Lung</creator><general>Nature Publishing Group UK</general><general>Nature Publishing Group</general><scope>C6C</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20160118</creationdate><title>Development of a luminescent G-quadruplex-selective iridium(III) complex for the label-free detection of adenosine</title><author>Lu, Lihua ; Zhong, Hai-Jing ; He, Bingyong ; Leung, Chung-Hang ; Ma, Dik-Lung</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c438t-64dfd0f463cb6ca1edf888120e6d8cc8667620bcf2742cd442565ed8a35809c73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>639/638/263/49</topic><topic>639/638/263/914</topic><topic>9/10</topic><topic>Adenosine</topic><topic>Aptamers</topic><topic>Biosensing Techniques</topic><topic>Chromatography</topic><topic>Coordination Complexes - chemistry</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>DNA probes</topic><topic>G-Quadruplexes</topic><topic>Humanities and Social Sciences</topic><topic>Iridium</topic><topic>Laboratories</topic><topic>Ligands</topic><topic>Luminescence</topic><topic>Luminescent Measurements</topic><topic>Molecular Structure</topic><topic>multidisciplinary</topic><topic>Nervous system</topic><topic>Nucleosides</topic><topic>Science</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lu, Lihua</creatorcontrib><creatorcontrib>Zhong, Hai-Jing</creatorcontrib><creatorcontrib>He, Bingyong</creatorcontrib><creatorcontrib>Leung, Chung-Hang</creatorcontrib><creatorcontrib>Ma, Dik-Lung</creatorcontrib><collection>Springer Nature OA Free Journals</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>ProQuest Health and Medical</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>ProQuest Science Journals</collection><collection>ProQuest Biological Science Journals</collection><collection>Publicly Available Content Database (Proquest) (PQ_SDU_P3)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Scientific reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lu, Lihua</au><au>Zhong, Hai-Jing</au><au>He, Bingyong</au><au>Leung, Chung-Hang</au><au>Ma, Dik-Lung</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Development of a luminescent G-quadruplex-selective iridium(III) complex for the label-free detection of adenosine</atitle><jtitle>Scientific reports</jtitle><stitle>Sci Rep</stitle><addtitle>Sci Rep</addtitle><date>2016-01-18</date><risdate>2016</risdate><volume>6</volume><issue>1</issue><spage>19368</spage><epage>19368</epage><pages>19368-19368</pages><artnum>19368</artnum><issn>2045-2322</issn><eissn>2045-2322</eissn><abstract>A panel of six luminescent iridium(III) complexes were synthesized and evaluated for their ability to act as G-quadruplex-selective probes. The novel iridium(III) complex
1
was found to be highly selective for G-quadruplex DNA and was employed for the construction of a label-free G-quadruplex-based adenosine detection assay in aqueous solution. Two different detection strategies were investigated for adenosine detection and the results showed that initial addition of adenosine to the adenosine aptamer gave superior results. The assay exhibited a linear response for adenosine in the concentration range of 5 to 120 μM (R
2
= 0.992) and the limit of detection for adenosine was 5 μM. Moreover, this assay was highly selective for adenosine over other nucleosides and exhibited potential use for biological sample analysis.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>26778273</pmid><doi>10.1038/srep19368</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | 639/638/263/49 639/638/263/914 9/10 Adenosine Aptamers Biosensing Techniques Chromatography Coordination Complexes - chemistry Deoxyribonucleic acid DNA DNA probes G-Quadruplexes Humanities and Social Sciences Iridium Laboratories Ligands Luminescence Luminescent Measurements Molecular Structure multidisciplinary Nervous system Nucleosides Science |
| title | Development of a luminescent G-quadruplex-selective iridium(III) complex for the label-free detection of adenosine |
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