Chlamydia gallinacea, not C. psittaci, is the endemic chlamydial species in chicken (Gallus gallus)
To investigate the prevalence and diversity of Chlamydia spp. in domestic birds in China, oral and cloacal swabs of healthy chickens, ducks, geese and pigeons were collected nationwide from live-animal markets and examined by Chlamydia spp. 23 S rRNA gene FRET-PCR followed by high-resolution melting...
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| Veröffentlicht in: | Scientific reports 2016-01, Vol.6 (1), p.19638-19638, Article 19638 |
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| creator | Guo, Weina Li, Jing Kaltenboeck, Bernhard Gong, Jiansen Fan, Weixing Wang, Chengming |
| description | To investigate the prevalence and diversity of
Chlamydia
spp. in domestic birds in China, oral and cloacal swabs of healthy chickens, ducks, geese and pigeons were collected nationwide from live-animal markets and examined by
Chlamydia
spp. 23 S rRNA gene FRET-PCR followed by high-resolution melting curve analysis and confirmatory sequencing. Overall, 26.2% of the birds (602/2,300) were positive for
Chlamydia
spp. and five
Chlamydia
spp. were identified. While occasional detection of
C. suis
and
C. muridarum
in poultry is reported here for the first time, the predominant chlamydial agent was
C
.
gallinacea
representing 63.8% of all positives (384/602) and 81.2% of positive chickens (359/442). Analysis of the
C. gallinacea ompA
phylogeny revealed at least 13 well segregated variants (serovars). Seven-month monitoring of
C. gallinacea
-infected chickens indicated that the infection was persistent.
C. gallinacea
-infected chickens remained without overt clinical disease, but showed body weight gains significantly reduced by 6.5–11.4% beginning in week 3 post-infection. This study indicates that
C. gallinacea
is the endemic chlamydial species in chickens, whereas
C. psittaci
dominates only in pigeons. Further studies are required to address the specific conditions under which
C. gallinacea
could act as an avian pathogen and possibly also a zoonotic agent. |
| doi_str_mv | 10.1038/srep19638 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_4726042</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1899043371</sourcerecordid><originalsourceid>FETCH-LOGICAL-c438t-e39175f9fd171888bd773237b29f4cd947194a31db8e4380030e6cc623db2bfa3</originalsourceid><addsrcrecordid>eNplkctKxDAUhoMoKurCF5CAGxVHc2uTbAQZvIHgRtchTU9nom1am1bw7Y2ODqNmk5DznS8n_AjtU3JGCVfnsYeO6pyrNbTNiMgmjDO2vnLeQnsxPpO0MqYF1Ztoi-VSKpLxbeSm89o276W3eGbr2gfrwJ7i0A54eoa76IfBOn-KfcTDHDCEEhrvsPvpqnHswHmI2Id0690LBHx0k1Rj_DKO8XgXbVS2jrD3ve-gp-urx-nt5P7h5m56eT9xgqthAlxTmVW6KqmkSqmilJIzLgumK-FKLSTVwnJaFgpSAyGcQO5cznhZsKKyfAddLLzdWDRQOghDb2vT9b6x_btprTe_K8HPzax9M0KynAiWBEffgr59HSEOpvHRQV3bAO0YDZU5USpL4yX08A_63I59SN8zVGlNBOdf1PGCcn0bU1DVchhKzGd6ZpleYg9Wp1-SP1kl4GQBxFQKM-hXnvxn-wBjRaMa</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1899043371</pqid></control><display><type>article</type><title>Chlamydia gallinacea, not C. psittaci, is the endemic chlamydial species in chicken (Gallus gallus)</title><source>MEDLINE</source><source>DOAJ Directory of Open Access Journals</source><source>Springer Nature OA Free Journals</source><source>Nature Free</source><source>EZB-FREE-00999 freely available EZB journals</source><source>PubMed Central</source><source>Free Full-Text Journals in Chemistry</source><creator>Guo, Weina ; Li, Jing ; Kaltenboeck, Bernhard ; Gong, Jiansen ; Fan, Weixing ; Wang, Chengming</creator><creatorcontrib>Guo, Weina ; Li, Jing ; Kaltenboeck, Bernhard ; Gong, Jiansen ; Fan, Weixing ; Wang, Chengming</creatorcontrib><description>To investigate the prevalence and diversity of
Chlamydia
spp. in domestic birds in China, oral and cloacal swabs of healthy chickens, ducks, geese and pigeons were collected nationwide from live-animal markets and examined by
Chlamydia
spp. 23 S rRNA gene FRET-PCR followed by high-resolution melting curve analysis and confirmatory sequencing. Overall, 26.2% of the birds (602/2,300) were positive for
Chlamydia
spp. and five
Chlamydia
spp. were identified. While occasional detection of
C. suis
and
C. muridarum
in poultry is reported here for the first time, the predominant chlamydial agent was
C
.
gallinacea
representing 63.8% of all positives (384/602) and 81.2% of positive chickens (359/442). Analysis of the
C. gallinacea ompA
phylogeny revealed at least 13 well segregated variants (serovars). Seven-month monitoring of
C. gallinacea
-infected chickens indicated that the infection was persistent.
C. gallinacea
-infected chickens remained without overt clinical disease, but showed body weight gains significantly reduced by 6.5–11.4% beginning in week 3 post-infection. This study indicates that
C. gallinacea
is the endemic chlamydial species in chickens, whereas
C. psittaci
dominates only in pigeons. Further studies are required to address the specific conditions under which
C. gallinacea
could act as an avian pathogen and possibly also a zoonotic agent.</description><identifier>ISSN: 2045-2322</identifier><identifier>EISSN: 2045-2322</identifier><identifier>DOI: 10.1038/srep19638</identifier><identifier>PMID: 26778053</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>692/4017 ; 692/699/255 ; Animals ; Biodiversity ; Body weight ; Body weight gain ; Chickens - microbiology ; China - epidemiology ; Chlamydia ; Chlamydia - classification ; Chlamydia - genetics ; Chlamydia gallinacea ; Chlamydia Infections - veterinary ; Columbidae ; Ducks ; Endemic species ; Fluorescence resonance energy transfer ; Gallus gallus ; Humanities and Social Sciences ; Melting curve ; multidisciplinary ; Phylogeny ; Polymerase Chain Reaction ; Polymorphism, Genetic ; Poultry Diseases - diagnosis ; Poultry Diseases - epidemiology ; Poultry Diseases - microbiology ; Prevalence ; rRNA ; Science ; Zoonoses</subject><ispartof>Scientific reports, 2016-01, Vol.6 (1), p.19638-19638, Article 19638</ispartof><rights>The Author(s) 2016</rights><rights>Copyright Nature Publishing Group Jan 2016</rights><rights>Copyright © 2016, Macmillan Publishers Limited 2016 Macmillan Publishers Limited</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c438t-e39175f9fd171888bd773237b29f4cd947194a31db8e4380030e6cc623db2bfa3</citedby><cites>FETCH-LOGICAL-c438t-e39175f9fd171888bd773237b29f4cd947194a31db8e4380030e6cc623db2bfa3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4726042/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4726042/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,27924,27925,41120,42189,51576,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26778053$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Guo, Weina</creatorcontrib><creatorcontrib>Li, Jing</creatorcontrib><creatorcontrib>Kaltenboeck, Bernhard</creatorcontrib><creatorcontrib>Gong, Jiansen</creatorcontrib><creatorcontrib>Fan, Weixing</creatorcontrib><creatorcontrib>Wang, Chengming</creatorcontrib><title>Chlamydia gallinacea, not C. psittaci, is the endemic chlamydial species in chicken (Gallus gallus)</title><title>Scientific reports</title><addtitle>Sci Rep</addtitle><addtitle>Sci Rep</addtitle><description>To investigate the prevalence and diversity of
Chlamydia
spp. in domestic birds in China, oral and cloacal swabs of healthy chickens, ducks, geese and pigeons were collected nationwide from live-animal markets and examined by
Chlamydia
spp. 23 S rRNA gene FRET-PCR followed by high-resolution melting curve analysis and confirmatory sequencing. Overall, 26.2% of the birds (602/2,300) were positive for
Chlamydia
spp. and five
Chlamydia
spp. were identified. While occasional detection of
C. suis
and
C. muridarum
in poultry is reported here for the first time, the predominant chlamydial agent was
C
.
gallinacea
representing 63.8% of all positives (384/602) and 81.2% of positive chickens (359/442). Analysis of the
C. gallinacea ompA
phylogeny revealed at least 13 well segregated variants (serovars). Seven-month monitoring of
C. gallinacea
-infected chickens indicated that the infection was persistent.
C. gallinacea
-infected chickens remained without overt clinical disease, but showed body weight gains significantly reduced by 6.5–11.4% beginning in week 3 post-infection. This study indicates that
C. gallinacea
is the endemic chlamydial species in chickens, whereas
C. psittaci
dominates only in pigeons. Further studies are required to address the specific conditions under which
C. gallinacea
could act as an avian pathogen and possibly also a zoonotic agent.</description><subject>692/4017</subject><subject>692/699/255</subject><subject>Animals</subject><subject>Biodiversity</subject><subject>Body weight</subject><subject>Body weight gain</subject><subject>Chickens - microbiology</subject><subject>China - epidemiology</subject><subject>Chlamydia</subject><subject>Chlamydia - classification</subject><subject>Chlamydia - genetics</subject><subject>Chlamydia gallinacea</subject><subject>Chlamydia Infections - veterinary</subject><subject>Columbidae</subject><subject>Ducks</subject><subject>Endemic species</subject><subject>Fluorescence resonance energy transfer</subject><subject>Gallus gallus</subject><subject>Humanities and Social Sciences</subject><subject>Melting curve</subject><subject>multidisciplinary</subject><subject>Phylogeny</subject><subject>Polymerase Chain Reaction</subject><subject>Polymorphism, Genetic</subject><subject>Poultry Diseases - diagnosis</subject><subject>Poultry Diseases - epidemiology</subject><subject>Poultry Diseases - microbiology</subject><subject>Prevalence</subject><subject>rRNA</subject><subject>Science</subject><subject>Zoonoses</subject><issn>2045-2322</issn><issn>2045-2322</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>C6C</sourceid><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNplkctKxDAUhoMoKurCF5CAGxVHc2uTbAQZvIHgRtchTU9nom1am1bw7Y2ODqNmk5DznS8n_AjtU3JGCVfnsYeO6pyrNbTNiMgmjDO2vnLeQnsxPpO0MqYF1Ztoi-VSKpLxbeSm89o276W3eGbr2gfrwJ7i0A54eoa76IfBOn-KfcTDHDCEEhrvsPvpqnHswHmI2Id0690LBHx0k1Rj_DKO8XgXbVS2jrD3ve-gp-urx-nt5P7h5m56eT9xgqthAlxTmVW6KqmkSqmilJIzLgumK-FKLSTVwnJaFgpSAyGcQO5cznhZsKKyfAddLLzdWDRQOghDb2vT9b6x_btprTe_K8HPzax9M0KynAiWBEffgr59HSEOpvHRQV3bAO0YDZU5USpL4yX08A_63I59SN8zVGlNBOdf1PGCcn0bU1DVchhKzGd6ZpleYg9Wp1-SP1kl4GQBxFQKM-hXnvxn-wBjRaMa</recordid><startdate>20160118</startdate><enddate>20160118</enddate><creator>Guo, Weina</creator><creator>Li, Jing</creator><creator>Kaltenboeck, Bernhard</creator><creator>Gong, Jiansen</creator><creator>Fan, Weixing</creator><creator>Wang, Chengming</creator><general>Nature Publishing Group UK</general><general>Nature Publishing Group</general><scope>C6C</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20160118</creationdate><title>Chlamydia gallinacea, not C. psittaci, is the endemic chlamydial species in chicken (Gallus gallus)</title><author>Guo, Weina ; Li, Jing ; Kaltenboeck, Bernhard ; Gong, Jiansen ; Fan, Weixing ; Wang, Chengming</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c438t-e39175f9fd171888bd773237b29f4cd947194a31db8e4380030e6cc623db2bfa3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>692/4017</topic><topic>692/699/255</topic><topic>Animals</topic><topic>Biodiversity</topic><topic>Body weight</topic><topic>Body weight gain</topic><topic>Chickens - microbiology</topic><topic>China - epidemiology</topic><topic>Chlamydia</topic><topic>Chlamydia - classification</topic><topic>Chlamydia - genetics</topic><topic>Chlamydia gallinacea</topic><topic>Chlamydia Infections - veterinary</topic><topic>Columbidae</topic><topic>Ducks</topic><topic>Endemic species</topic><topic>Fluorescence resonance energy transfer</topic><topic>Gallus gallus</topic><topic>Humanities and Social Sciences</topic><topic>Melting curve</topic><topic>multidisciplinary</topic><topic>Phylogeny</topic><topic>Polymerase Chain Reaction</topic><topic>Polymorphism, Genetic</topic><topic>Poultry Diseases - diagnosis</topic><topic>Poultry Diseases - epidemiology</topic><topic>Poultry Diseases - microbiology</topic><topic>Prevalence</topic><topic>rRNA</topic><topic>Science</topic><topic>Zoonoses</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Guo, Weina</creatorcontrib><creatorcontrib>Li, Jing</creatorcontrib><creatorcontrib>Kaltenboeck, Bernhard</creatorcontrib><creatorcontrib>Gong, Jiansen</creatorcontrib><creatorcontrib>Fan, Weixing</creatorcontrib><creatorcontrib>Wang, Chengming</creatorcontrib><collection>Springer Nature OA Free Journals</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Biological Science Database</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Scientific reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Guo, Weina</au><au>Li, Jing</au><au>Kaltenboeck, Bernhard</au><au>Gong, Jiansen</au><au>Fan, Weixing</au><au>Wang, Chengming</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Chlamydia gallinacea, not C. psittaci, is the endemic chlamydial species in chicken (Gallus gallus)</atitle><jtitle>Scientific reports</jtitle><stitle>Sci Rep</stitle><addtitle>Sci Rep</addtitle><date>2016-01-18</date><risdate>2016</risdate><volume>6</volume><issue>1</issue><spage>19638</spage><epage>19638</epage><pages>19638-19638</pages><artnum>19638</artnum><issn>2045-2322</issn><eissn>2045-2322</eissn><abstract>To investigate the prevalence and diversity of
Chlamydia
spp. in domestic birds in China, oral and cloacal swabs of healthy chickens, ducks, geese and pigeons were collected nationwide from live-animal markets and examined by
Chlamydia
spp. 23 S rRNA gene FRET-PCR followed by high-resolution melting curve analysis and confirmatory sequencing. Overall, 26.2% of the birds (602/2,300) were positive for
Chlamydia
spp. and five
Chlamydia
spp. were identified. While occasional detection of
C. suis
and
C. muridarum
in poultry is reported here for the first time, the predominant chlamydial agent was
C
.
gallinacea
representing 63.8% of all positives (384/602) and 81.2% of positive chickens (359/442). Analysis of the
C. gallinacea ompA
phylogeny revealed at least 13 well segregated variants (serovars). Seven-month monitoring of
C. gallinacea
-infected chickens indicated that the infection was persistent.
C. gallinacea
-infected chickens remained without overt clinical disease, but showed body weight gains significantly reduced by 6.5–11.4% beginning in week 3 post-infection. This study indicates that
C. gallinacea
is the endemic chlamydial species in chickens, whereas
C. psittaci
dominates only in pigeons. Further studies are required to address the specific conditions under which
C. gallinacea
could act as an avian pathogen and possibly also a zoonotic agent.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>26778053</pmid><doi>10.1038/srep19638</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; DOAJ Directory of Open Access Journals; Springer Nature OA Free Journals; Nature Free; EZB-FREE-00999 freely available EZB journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | 692/4017 692/699/255 Animals Biodiversity Body weight Body weight gain Chickens - microbiology China - epidemiology Chlamydia Chlamydia - classification Chlamydia - genetics Chlamydia gallinacea Chlamydia Infections - veterinary Columbidae Ducks Endemic species Fluorescence resonance energy transfer Gallus gallus Humanities and Social Sciences Melting curve multidisciplinary Phylogeny Polymerase Chain Reaction Polymorphism, Genetic Poultry Diseases - diagnosis Poultry Diseases - epidemiology Poultry Diseases - microbiology Prevalence rRNA Science Zoonoses |
| title | Chlamydia gallinacea, not C. psittaci, is the endemic chlamydial species in chicken (Gallus gallus) |
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