Transfection and expression of hepatitis B virus x gene and its effect on apoptosis in HL-7702 cells

AIM: To investigate the effects of hepatitis B virus x gene and its protein product HBxAg on apoptosis in hepatocyte line HL-7702. METHODS: The reconstituted plasmid pcDNA3-x was established through recombination DNA technique; pcDNA3X was transfected into HL-7702 cells by lipid-mediated trasfection...

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Veröffentlicht in:	World journal of gastroenterology : WJG 2004-04, Vol.10 (7), p.959-964
Hauptverfasser:	Chen, Hong-Ying, Tang, Nan-Hong, Li, Xiu-Jin, Zhang, Sheng-Jun, Chen, Zhi-Xin, Wang, Xiao-Zhong
Format:	Artikel
Sprache:	eng
Schlagworte:	Apoptosis - drug effects Cell Line Flow Cytometry Gene Expression Hepatocytes - drug effects Hepatocytes - physiology Hepatocytes - ultrastructure HL-7702细胞 Liver Cancer Microscopy, Electron Trans-Activators - genetics Trans-Activators - pharmacology Transfection TUNEL法 X基因乙型肝炎病毒基因转染细胞凋亡
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