Isolation and Transmission of the Lyme Disease Spirochete from the Southeastern United States
The isolation of the Lyme disease spirochete (Borrelia burgdorferi) from the southeastern United States is reported. Three isolates, two from cotton mice (Peromyscus gossypinus) and one from the black-legged tick (Ixodes scapularis), were recovered from Sapelo Island, Georgia, in July and September...
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| Veröffentlicht in: | Proceedings of the National Academy of Sciences - PNAS 1993-08, Vol.90 (15), p.7371-7375 |
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| container_title | Proceedings of the National Academy of Sciences - PNAS |
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| creator | Oliver, J. H. Chandler, F. W. Luttrell, M. P. James, A. M. Stallknecht, D. E. McGuire, B. S. Hutcheson, H. J. Cummins, G. A. Lane, R. S. |
| description | The isolation of the Lyme disease spirochete (Borrelia burgdorferi) from the southeastern United States is reported. Three isolates, two from cotton mice (Peromyscus gossypinus) and one from the black-legged tick (Ixodes scapularis), were recovered from Sapelo Island, Georgia, in July and September 1991. The spirochetes were characterized by indirect fluorescent antibody assay using a battery of five monoclonal antibodies, by sodium dodecyl sulfate/polyacrylamide gel electrophoresis (SDS/PAGE) of whole cell lysates, and by the polymerase chain reaction (PCR) assay using primers for three DNA target sequences found in B. burgdorferi reference strain B-31. Transmission experiments indicate that the three Georgia isolates can infect experimentally inoculated hamsters and mice. Tick transmission of one of the isolates has been attempted so far; I. scapularis transmitted isolate SI-1 from hamsters to mice, but the lone-star tick, Amblyomma americanum, did not. |
| doi_str_mv | 10.1073/pnas.90.15.7371 |
| format | Article |
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H. ; Chandler, F. W. ; Luttrell, M. P. ; James, A. M. ; Stallknecht, D. E. ; McGuire, B. S. ; Hutcheson, H. J. ; Cummins, G. A. ; Lane, R. S.</creator><creatorcontrib>Oliver, J. H. ; Chandler, F. W. ; Luttrell, M. P. ; James, A. M. ; Stallknecht, D. E. ; McGuire, B. S. ; Hutcheson, H. J. ; Cummins, G. A. ; Lane, R. S.</creatorcontrib><description>The isolation of the Lyme disease spirochete (Borrelia burgdorferi) from the southeastern United States is reported. Three isolates, two from cotton mice (Peromyscus gossypinus) and one from the black-legged tick (Ixodes scapularis), were recovered from Sapelo Island, Georgia, in July and September 1991. The spirochetes were characterized by indirect fluorescent antibody assay using a battery of five monoclonal antibodies, by sodium dodecyl sulfate/polyacrylamide gel electrophoresis (SDS/PAGE) of whole cell lysates, and by the polymerase chain reaction (PCR) assay using primers for three DNA target sequences found in B. burgdorferi reference strain B-31. Transmission experiments indicate that the three Georgia isolates can infect experimentally inoculated hamsters and mice. Tick transmission of one of the isolates has been attempted so far; I. scapularis transmitted isolate SI-1 from hamsters to mice, but the lone-star tick, Amblyomma americanum, did not.</description><identifier>ISSN: 0027-8424</identifier><identifier>EISSN: 1091-6490</identifier><identifier>DOI: 10.1073/pnas.90.15.7371</identifier><identifier>PMID: 8346258</identifier><identifier>CODEN: PNASA6</identifier><language>eng</language><publisher>Washington, DC: National Academy of Sciences of the United States of America</publisher><subject>Animals ; Animals, Wild - microbiology ; Antibodies ; Antigens, Surface - genetics ; Arachnid Vectors - microbiology ; Bacterial Outer Membrane Proteins - genetics ; Bacterial Vaccines ; Bacteriology ; Base Sequence ; Biological and medical sciences ; Borrelia burgdorferi ; Borrelia burgdorferi Group - isolation & purification ; DNA, Bacterial - analysis ; Fundamental and applied biological sciences. Psychology ; Gels ; Georgia ; Hamsters ; Ixodes scapularis ; Larvae ; Lipoproteins ; Lyme disease ; Lyme Disease - microbiology ; Medical research ; Mice ; Microbiology ; Molecular Sequence Data ; Nymphs ; Oligodeoxyribonucleotides - chemistry ; Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains ; Peromyscus - microbiology ; Peromyscus gossypinus ; Polymerase Chain Reaction ; Spirochaetales ; Ticks ; Ticks - microbiology</subject><ispartof>Proceedings of the National Academy of Sciences - PNAS, 1993-08, Vol.90 (15), p.7371-7375</ispartof><rights>Copyright 1993 The National Academy of Sciences of the United States of America</rights><rights>1993 INIST-CNRS</rights><rights>Copyright National Academy of Sciences Aug 1, 1993</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c549t-b379f82658d6b455481082777da7967237f3fb21c3d6452b9e6cf35795ab18513</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.pnas.org/content/90/15.cover.gif</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/2362715$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/2362715$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,723,776,780,799,881,27901,27902,53766,53768,57992,58225</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4908340$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8346258$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Oliver, J. H.</creatorcontrib><creatorcontrib>Chandler, F. W.</creatorcontrib><creatorcontrib>Luttrell, M. P.</creatorcontrib><creatorcontrib>James, A. M.</creatorcontrib><creatorcontrib>Stallknecht, D. E.</creatorcontrib><creatorcontrib>McGuire, B. S.</creatorcontrib><creatorcontrib>Hutcheson, H. J.</creatorcontrib><creatorcontrib>Cummins, G. A.</creatorcontrib><creatorcontrib>Lane, R. S.</creatorcontrib><title>Isolation and Transmission of the Lyme Disease Spirochete from the Southeastern United States</title><title>Proceedings of the National Academy of Sciences - PNAS</title><addtitle>Proc Natl Acad Sci U S A</addtitle><description>The isolation of the Lyme disease spirochete (Borrelia burgdorferi) from the southeastern United States is reported. Three isolates, two from cotton mice (Peromyscus gossypinus) and one from the black-legged tick (Ixodes scapularis), were recovered from Sapelo Island, Georgia, in July and September 1991. The spirochetes were characterized by indirect fluorescent antibody assay using a battery of five monoclonal antibodies, by sodium dodecyl sulfate/polyacrylamide gel electrophoresis (SDS/PAGE) of whole cell lysates, and by the polymerase chain reaction (PCR) assay using primers for three DNA target sequences found in B. burgdorferi reference strain B-31. Transmission experiments indicate that the three Georgia isolates can infect experimentally inoculated hamsters and mice. Tick transmission of one of the isolates has been attempted so far; I. scapularis transmitted isolate SI-1 from hamsters to mice, but the lone-star tick, Amblyomma americanum, did not.</description><subject>Animals</subject><subject>Animals, Wild - microbiology</subject><subject>Antibodies</subject><subject>Antigens, Surface - genetics</subject><subject>Arachnid Vectors - microbiology</subject><subject>Bacterial Outer Membrane Proteins - genetics</subject><subject>Bacterial Vaccines</subject><subject>Bacteriology</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Borrelia burgdorferi</subject><subject>Borrelia burgdorferi Group - isolation & purification</subject><subject>DNA, Bacterial - analysis</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gels</subject><subject>Georgia</subject><subject>Hamsters</subject><subject>Ixodes scapularis</subject><subject>Larvae</subject><subject>Lipoproteins</subject><subject>Lyme disease</subject><subject>Lyme Disease - microbiology</subject><subject>Medical research</subject><subject>Mice</subject><subject>Microbiology</subject><subject>Molecular Sequence Data</subject><subject>Nymphs</subject><subject>Oligodeoxyribonucleotides - chemistry</subject><subject>Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains</subject><subject>Peromyscus - microbiology</subject><subject>Peromyscus gossypinus</subject><subject>Polymerase Chain Reaction</subject><subject>Spirochaetales</subject><subject>Ticks</subject><subject>Ticks - microbiology</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1993</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkstv1DAQhy0EKkvhzAVQhCo4ZetH_JK4VKWFSitx2PaILCex2awSe2s7qP3vcdh0RTnAaTSeb54_A_AawSWCnJzunI5LmR265ISjJ2CBoEQlqyR8ChYQYl6KClfPwYsYtxBCSQU8AkeCVAxTsQDfr6Lvdeq8K7Rri-ugXRy6GKcHb4u0McXqfjDF5y4aHU2x3nXBNxuTTGGDH34Daz9mo2MywRU3rkumLdZJJxNfgmdW99G8mu0xuLm8uD7_Wq6-fbk6P1uVDa1kKmvCpRWYUdGyuqK0EggKzDlvNZeMY8ItsTVGDWlZRXEtDWssoVxSXSNBETkGn_Z1d2M9mLYxLgXdq13oBh3uldedehxx3Ub98D9VxRGROf3DnB787WhiUvkEjel77Ywfo-JUyHxc-F8QMcby8DyD7_8Ct34MLt9AYYgwQ5BObU_3UBN8jMHYw8AIqkldNamrZHaomtTNGW__3PPAz3Lm-Mkc17HRvc1qNl08YPlTZHJa492MTfUfoo_6fPwnoOzY98ncpUy-2ZPbmHw4oJgwzBElvwChac6Y</recordid><startdate>19930801</startdate><enddate>19930801</enddate><creator>Oliver, J. 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Psychology</topic><topic>Gels</topic><topic>Georgia</topic><topic>Hamsters</topic><topic>Ixodes scapularis</topic><topic>Larvae</topic><topic>Lipoproteins</topic><topic>Lyme disease</topic><topic>Lyme Disease - microbiology</topic><topic>Medical research</topic><topic>Mice</topic><topic>Microbiology</topic><topic>Molecular Sequence Data</topic><topic>Nymphs</topic><topic>Oligodeoxyribonucleotides - chemistry</topic><topic>Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains</topic><topic>Peromyscus - microbiology</topic><topic>Peromyscus gossypinus</topic><topic>Polymerase Chain Reaction</topic><topic>Spirochaetales</topic><topic>Ticks</topic><topic>Ticks - microbiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Oliver, J. H.</creatorcontrib><creatorcontrib>Chandler, F. W.</creatorcontrib><creatorcontrib>Luttrell, M. P.</creatorcontrib><creatorcontrib>James, A. 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H.</au><au>Chandler, F. W.</au><au>Luttrell, M. P.</au><au>James, A. M.</au><au>Stallknecht, D. E.</au><au>McGuire, B. S.</au><au>Hutcheson, H. J.</au><au>Cummins, G. A.</au><au>Lane, R. S.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Isolation and Transmission of the Lyme Disease Spirochete from the Southeastern United States</atitle><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle><addtitle>Proc Natl Acad Sci U S A</addtitle><date>1993-08-01</date><risdate>1993</risdate><volume>90</volume><issue>15</issue><spage>7371</spage><epage>7375</epage><pages>7371-7375</pages><issn>0027-8424</issn><eissn>1091-6490</eissn><coden>PNASA6</coden><abstract>The isolation of the Lyme disease spirochete (Borrelia burgdorferi) from the southeastern United States is reported. Three isolates, two from cotton mice (Peromyscus gossypinus) and one from the black-legged tick (Ixodes scapularis), were recovered from Sapelo Island, Georgia, in July and September 1991. The spirochetes were characterized by indirect fluorescent antibody assay using a battery of five monoclonal antibodies, by sodium dodecyl sulfate/polyacrylamide gel electrophoresis (SDS/PAGE) of whole cell lysates, and by the polymerase chain reaction (PCR) assay using primers for three DNA target sequences found in B. burgdorferi reference strain B-31. Transmission experiments indicate that the three Georgia isolates can infect experimentally inoculated hamsters and mice. Tick transmission of one of the isolates has been attempted so far; I. scapularis transmitted isolate SI-1 from hamsters to mice, but the lone-star tick, Amblyomma americanum, did not.</abstract><cop>Washington, DC</cop><pub>National Academy of Sciences of the United States of America</pub><pmid>8346258</pmid><doi>10.1073/pnas.90.15.7371</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Proceedings of the National Academy of Sciences - PNAS, 1993-08, Vol.90 (15), p.7371-7375 |
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| source | Jstor Complete Legacy; MEDLINE; PubMed Central; Alma/SFX Local Collection; Free Full-Text Journals in Chemistry |
| subjects | Animals Animals, Wild - microbiology Antibodies Antigens, Surface - genetics Arachnid Vectors - microbiology Bacterial Outer Membrane Proteins - genetics Bacterial Vaccines Bacteriology Base Sequence Biological and medical sciences Borrelia burgdorferi Borrelia burgdorferi Group - isolation & purification DNA, Bacterial - analysis Fundamental and applied biological sciences. Psychology Gels Georgia Hamsters Ixodes scapularis Larvae Lipoproteins Lyme disease Lyme Disease - microbiology Medical research Mice Microbiology Molecular Sequence Data Nymphs Oligodeoxyribonucleotides - chemistry Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains Peromyscus - microbiology Peromyscus gossypinus Polymerase Chain Reaction Spirochaetales Ticks Ticks - microbiology |
| title | Isolation and Transmission of the Lyme Disease Spirochete from the Southeastern United States |
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