Sequencing of lariat termini in S. cerevisiae reveals 5' splice sites, branch points, and novel splicing events

Pre-mRNA splicing is a central step in the shaping of the eukaryotic transcriptome and in the regulation of gene expression. Yet, due to a focus on fully processed mRNA, common approaches for defining pre-mRNA splicing genome-wide are suboptimal-especially with respect to defining the branch point s...

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