Construction of a subunit-fusion nitrile hydratase and discovery of an innovative metal ion transfer pattern

Metallochaperones are metal-binding proteins designed to deliver the appropriate metal to a target protein. The metal is usually transferred between different proteins. In this study, we discovered that metal was transferred between the same subunit of a mutant nitrile hydratase (NHase). Various “ac...

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Veröffentlicht in:	Scientific reports 2016-01, Vol.6 (1), p.19183-19183, Article 19183
Hauptverfasser:	Xia, Yuanyuan, Cui, Wenjing, Liu, Zhongmei, Zhou, Li, Cui, Youtian, Kobayashi, Michihiko, Zhou, Zhemin
Format:	Artikel
Sprache:	eng
Schlagworte:	38/109 38/23 38/44 38/77 631/337/149 631/45/173 82/16 82/29 82/80 82/83 Biological Transport Biosynthesis Cobalt Enzyme Activation Humanities and Social Sciences Hydro-Lyases - chemistry Hydro-Lyases - genetics Hydro-Lyases - isolation & purification Hydro-Lyases - metabolism Ions Ions - metabolism Mass Spectrometry Metal ions Metals - metabolism Models, Biological Molecular Weight multidisciplinary Nitrile hydratase Protein Binding Protein Subunits - genetics Protein Subunits - metabolism Proteins Recombinant Fusion Proteins Science Thermal stability
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