Genome Editing in Human Pluripotent Stem Cells: Approaches, Pitfalls, and Solutions
Human pluripotent stem cells (hPSCs) with knockout or mutant alleles can be generated using custom-engineered nucleases. Transcription activator-like effector nucleases (TALENs) and clustered regularly interspaced short palindromic repeats (CRISPR)-Cas9 nucleases are the most commonly employed techn...
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| Veröffentlicht in: | Cell stem cell 2016-01, Vol.18 (1), p.53-65 |
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| creator | Hendriks, William T. Warren, Curtis R. Cowan, Chad A. |
| description | Human pluripotent stem cells (hPSCs) with knockout or mutant alleles can be generated using custom-engineered nucleases. Transcription activator-like effector nucleases (TALENs) and clustered regularly interspaced short palindromic repeats (CRISPR)-Cas9 nucleases are the most commonly employed technologies for editing hPSC genomes. In this Protocol Review, we provide a brief overview of custom-engineered nucleases in the context of gene editing in hPSCs with a focus on the application of TALENs and CRISPR/Cas9. We will highlight the advantages and disadvantages of each method and discuss theoretical and technical considerations for experimental design.
In this Protocol Review, Hendriks et al. provide a brief overview of custom-engineered nucleases for gene editing in hPSCs with a focus on the application of TALENs and CRISPR/Cas9. They highlight the advantages and disadvantages of each method and discuss theoretical and technical considerations for experimental design. |
| doi_str_mv | 10.1016/j.stem.2015.12.002 |
| format | Article |
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| ispartof | Cell stem cell, 2016-01, Vol.18 (1), p.53-65 |
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| language | eng |
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| source | MEDLINE; Cell Press Free Archives; Elsevier ScienceDirect Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | Alleles CRISPR-Cas Systems Deoxyribonucleases - genetics Deoxyribonucleases - metabolism DNA - analysis Genetic Techniques Genetic Vectors Genome, Human Genotype Humans Mutation Pluripotent Stem Cells - cytology Point Mutation Sequence Homology, Nucleic Acid |
| title | Genome Editing in Human Pluripotent Stem Cells: Approaches, Pitfalls, and Solutions |
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