A Revised Mechanism for Human Cyclooxygenase-2

The mechanism of ω-6 polyunsaturated fatty acid oxidation by wild-type cyclooxygenase 2 and the Y334F variant, lacking a conserved hydrogen bond to the catalytic tyrosyl radical/tyrosine, was examined for the first time under physiologically relevant conditions. The enzymes show apparent bimolecular...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	The Journal of biological chemistry 2016-01, Vol.291 (2), p.948-958
Hauptverfasser:	Liu, Yi, Roth, Justine P.
Format:	Artikel
Sprache:	eng
Schlagworte:	Arachidonic Acid - metabolism Chromatography, High Pressure Liquid Cyclooxygenase 2 - chemistry Cyclooxygenase 2 - metabolism Deuterium - metabolism dioxygenase enzyme mechanism Enzymology fatty acid Humans isotope effect Kinetics Linoleic Acid - metabolism Mass Spectrometry Mutant Proteins - metabolism Oxidation-Reduction Oxygen Consumption prostaglandin Solvents Temperature Viscosity
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Schreiben Sie den ersten Kommentar!