Development of an Intracellular Screen for New Compounds Able To Inhibit Mycobacterium tuberculosis Growth in Human Macrophages

Here we describe the development and validation of an intracellular high-throughput screening assay for finding new antituberculosis compounds active in human macrophages. The assay consists of a luciferase-based primary identification assay, followed by a green fluorescent protein-based secondary p...

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Veröffentlicht in:	Antimicrobial agents and chemotherapy 2016-01, Vol.60 (1), p.640-645
Hauptverfasser:	Sorrentino, Flavia, Gonzalez del Rio, Ruben, Zheng, Xingji, Presa Matilla, Jesus, Torres Gomez, Pedro, Martinez Hoyos, Maria, Perez Herran, Maria Esther, Mendoza Losana, Alfonso, Av-Gay, Yossef
Format:	Artikel
Sprache:	eng
Schlagworte:	Antitubercular Agents Antitubercular Agents - chemistry Antitubercular Agents - pharmacology Cell Line Experimental Therapeutics Gene Expression Genes, Reporter Green Fluorescent Proteins - genetics Green Fluorescent Proteins - metabolism Hep G2 Cells High-Throughput Screening Assays Humans Luciferases - genetics Luciferases - metabolism Macrophages Macrophages - drug effects Macrophages - microbiology Microbial Sensitivity Tests Mycobacterium tuberculosis Mycobacterium tuberculosis - drug effects Mycobacterium tuberculosis - growth & development Structure-Activity Relationship
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