MicroRNA expression analysis in high fat diet-induced NAFLD-NASH-HCC progression: study on C57BL/6J mice
Hepatocellular carcinoma (HCC) is the most common malignant tumor of the liver. Non-alcoholic fatty liver disease (NAFLD) is a frequent chronic liver disorder in developed countries. NAFLD can progress through the more severe non alcoholic steatohepatitis (NASH), cirrhosis and, lastly, HCC. Genetic...
Gespeichert in:
Veröffentlicht in: | BMC cancer 2016-01, Vol.16 (3), p.3-3, Article 3 |
---|---|
Hauptverfasser: | , , , , , , , , , , , , |
Format: | Artikel |
Sprache: | eng |
Schlagworte: | |
Online-Zugang: | Volltext |
Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
container_end_page | 3 |
---|---|
container_issue | 3 |
container_start_page | 3 |
container_title | BMC cancer |
container_volume | 16 |
creator | Tessitore, Alessandra Cicciarelli, Germana Del Vecchio, Filippo Gaggiano, Agata Verzella, Daniela Fischietti, Mariafausta Mastroiaco, Valentina Vetuschi, Antonella Sferra, Roberta Barnabei, Remo Capece, Daria Zazzeroni, Francesca Alesse, Edoardo |
description | Hepatocellular carcinoma (HCC) is the most common malignant tumor of the liver. Non-alcoholic fatty liver disease (NAFLD) is a frequent chronic liver disorder in developed countries. NAFLD can progress through the more severe non alcoholic steatohepatitis (NASH), cirrhosis and, lastly, HCC. Genetic and epigenetic alterations of coding genes as well as deregulation of microRNAs (miRNAs) activity play a role in HCC development. In this study, the C57BL/6J mouse model was long term high-fat (HF) or low-fat (LF) diet fed, in order to analyze molecular mechanisms responsible for the hepatic damage progression.
Mice were HF or LF diet fed for different time points, then plasma and hepatic tissues were collected. Histological and clinical chemistry assays were performed to assess the progression of liver disease. MicroRNAs' differential expression was evaluated on pooled RNAs from tissues, and some miRNAs showing dysregulation were further analyzed at the individual level.
Cholesterol, low and high density lipoproteins, triglycerides and alanine aminotransferase increase was detected in HF mice. Gross anatomical examination revealed hepatomegaly in HF livers, and histological analysis highlighted different degrees and levels of steatosis, inflammatory infiltrate and fibrosis in HF and LF animals, demonstrating the progression from NAFLD through NASH. Macroscopic nodules, showing typical neoplastic features, were observed in 20% of HF diet fed mice. Fifteen miRNAs differentially expressed in HF with respect to LF hepatic tissues during the progression of liver damage, and in tumors with respect to HF non tumor liver specimens were identified. Among them, miR-340-5p, miR-484, miR-574-3p, miR-720, whose expression was never described in NAFLD, NASH and HCC tissues, and miR-125a-5p and miR-182, which showed early and significant dysregulation in the sequential hepatic damage process.
In this study, fifteen microRNAs which were modulated in hepatic tissues and in tumors during the transition NAFLD-NASH-HCC are reported. Besides some already described, new and early dysregulated miRNAs were identified. Functional analyses are needed to validate the results here obtained, and to better define the role of these molecules in the progression of the hepatic disease. |
doi_str_mv | 10.1186/s12885-015-2007-1 |
format | Article |
fullrecord | <record><control><sourceid>gale_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_4700747</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A451358865</galeid><sourcerecordid>A451358865</sourcerecordid><originalsourceid>FETCH-LOGICAL-c667t-58585b9dae5b5f084f24c02dc95d32b4329fb3027a88f61bc01a0956b02fe3b3</originalsourceid><addsrcrecordid>eNptkkGL1DAcxYMo7u7oB_AiAUH0kN0kTZrUg1Cr66yMI-zuPaRp2kY6zdi0y863N8OMyxQkhITk997h_R8Abwi-JESmV4FQKTnChCOKsUDkGTgnTBBEGRbPT-5n4CKE3xgTIbF8Cc5oKqjEjJ2D9qczg79d59A-bgcbgvM91L3udsEF6HrYuqaFtR5h5eyIXF9NxlZwnV-vvqJ1frdEy6KA28E3R_EnGMap2sFoU3DxZXWV_oAbZ-wr8KLWXbCvj-cC3F9_uy-WaPXr-02Rr5BJUzEiLuMqs0pbXvIaS1ZTZjCtTMarhJYsoVldJpgKLWWdktJgonHG0xLT2iZlsgCfD7bbqdzYyth-HHSntoPb6GGnvHZq_tO7VjX-QTERE2QiGnw4Ggz-z2TDqDYuGNt1urd-CooIznCWyLgX4N0BbXRnletrHx3NHlc54yThUqY8Upf_oeKqbMzF97Z28X0m-DgTRGa0j2OjpxDUzd3tnH1_wrZWd2MbfDeNcRJhDpIDGKcdwmDrp0gIVvsyqUOZVCyT2pdJkah5e5rlk-Jfe5K_20TA0A</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1754093809</pqid></control><display><type>article</type><title>MicroRNA expression analysis in high fat diet-induced NAFLD-NASH-HCC progression: study on C57BL/6J mice</title><source>MEDLINE</source><source>DOAJ Directory of Open Access Journals</source><source>SpringerNature Journals</source><source>PubMed Central Open Access</source><source>Springer Nature OA Free Journals</source><source>EZB-FREE-00999 freely available EZB journals</source><source>PubMed Central</source><creator>Tessitore, Alessandra ; Cicciarelli, Germana ; Del Vecchio, Filippo ; Gaggiano, Agata ; Verzella, Daniela ; Fischietti, Mariafausta ; Mastroiaco, Valentina ; Vetuschi, Antonella ; Sferra, Roberta ; Barnabei, Remo ; Capece, Daria ; Zazzeroni, Francesca ; Alesse, Edoardo</creator><creatorcontrib>Tessitore, Alessandra ; Cicciarelli, Germana ; Del Vecchio, Filippo ; Gaggiano, Agata ; Verzella, Daniela ; Fischietti, Mariafausta ; Mastroiaco, Valentina ; Vetuschi, Antonella ; Sferra, Roberta ; Barnabei, Remo ; Capece, Daria ; Zazzeroni, Francesca ; Alesse, Edoardo</creatorcontrib><description>Hepatocellular carcinoma (HCC) is the most common malignant tumor of the liver. Non-alcoholic fatty liver disease (NAFLD) is a frequent chronic liver disorder in developed countries. NAFLD can progress through the more severe non alcoholic steatohepatitis (NASH), cirrhosis and, lastly, HCC. Genetic and epigenetic alterations of coding genes as well as deregulation of microRNAs (miRNAs) activity play a role in HCC development. In this study, the C57BL/6J mouse model was long term high-fat (HF) or low-fat (LF) diet fed, in order to analyze molecular mechanisms responsible for the hepatic damage progression.
Mice were HF or LF diet fed for different time points, then plasma and hepatic tissues were collected. Histological and clinical chemistry assays were performed to assess the progression of liver disease. MicroRNAs' differential expression was evaluated on pooled RNAs from tissues, and some miRNAs showing dysregulation were further analyzed at the individual level.
Cholesterol, low and high density lipoproteins, triglycerides and alanine aminotransferase increase was detected in HF mice. Gross anatomical examination revealed hepatomegaly in HF livers, and histological analysis highlighted different degrees and levels of steatosis, inflammatory infiltrate and fibrosis in HF and LF animals, demonstrating the progression from NAFLD through NASH. Macroscopic nodules, showing typical neoplastic features, were observed in 20% of HF diet fed mice. Fifteen miRNAs differentially expressed in HF with respect to LF hepatic tissues during the progression of liver damage, and in tumors with respect to HF non tumor liver specimens were identified. Among them, miR-340-5p, miR-484, miR-574-3p, miR-720, whose expression was never described in NAFLD, NASH and HCC tissues, and miR-125a-5p and miR-182, which showed early and significant dysregulation in the sequential hepatic damage process.
In this study, fifteen microRNAs which were modulated in hepatic tissues and in tumors during the transition NAFLD-NASH-HCC are reported. Besides some already described, new and early dysregulated miRNAs were identified. Functional analyses are needed to validate the results here obtained, and to better define the role of these molecules in the progression of the hepatic disease.</description><identifier>ISSN: 1471-2407</identifier><identifier>EISSN: 1471-2407</identifier><identifier>DOI: 10.1186/s12885-015-2007-1</identifier><identifier>PMID: 26728044</identifier><language>eng</language><publisher>England: BioMed Central Ltd</publisher><subject>Analysis ; Animals ; Carcinoma, Hepatocellular - genetics ; Carcinoma, Hepatocellular - pathology ; Cell Line, Tumor ; Cholesterol - blood ; Diet, High-Fat ; Disease Models, Animal ; Disease Progression ; Gene Expression Regulation, Neoplastic ; Hepatoma ; Humans ; Liver Neoplasms - genetics ; Liver Neoplasms - pathology ; Mice ; MicroRNA ; MicroRNAs - biosynthesis ; MicroRNAs - genetics ; Non-alcoholic Fatty Liver Disease - genetics ; Non-alcoholic Fatty Liver Disease - pathology ; Risk factors ; Triglycerides - blood</subject><ispartof>BMC cancer, 2016-01, Vol.16 (3), p.3-3, Article 3</ispartof><rights>COPYRIGHT 2016 BioMed Central Ltd.</rights><rights>Tessitore et al. 2015</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c667t-58585b9dae5b5f084f24c02dc95d32b4329fb3027a88f61bc01a0956b02fe3b3</citedby><cites>FETCH-LOGICAL-c667t-58585b9dae5b5f084f24c02dc95d32b4329fb3027a88f61bc01a0956b02fe3b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4700747/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4700747/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26728044$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Tessitore, Alessandra</creatorcontrib><creatorcontrib>Cicciarelli, Germana</creatorcontrib><creatorcontrib>Del Vecchio, Filippo</creatorcontrib><creatorcontrib>Gaggiano, Agata</creatorcontrib><creatorcontrib>Verzella, Daniela</creatorcontrib><creatorcontrib>Fischietti, Mariafausta</creatorcontrib><creatorcontrib>Mastroiaco, Valentina</creatorcontrib><creatorcontrib>Vetuschi, Antonella</creatorcontrib><creatorcontrib>Sferra, Roberta</creatorcontrib><creatorcontrib>Barnabei, Remo</creatorcontrib><creatorcontrib>Capece, Daria</creatorcontrib><creatorcontrib>Zazzeroni, Francesca</creatorcontrib><creatorcontrib>Alesse, Edoardo</creatorcontrib><title>MicroRNA expression analysis in high fat diet-induced NAFLD-NASH-HCC progression: study on C57BL/6J mice</title><title>BMC cancer</title><addtitle>BMC Cancer</addtitle><description>Hepatocellular carcinoma (HCC) is the most common malignant tumor of the liver. Non-alcoholic fatty liver disease (NAFLD) is a frequent chronic liver disorder in developed countries. NAFLD can progress through the more severe non alcoholic steatohepatitis (NASH), cirrhosis and, lastly, HCC. Genetic and epigenetic alterations of coding genes as well as deregulation of microRNAs (miRNAs) activity play a role in HCC development. In this study, the C57BL/6J mouse model was long term high-fat (HF) or low-fat (LF) diet fed, in order to analyze molecular mechanisms responsible for the hepatic damage progression.
Mice were HF or LF diet fed for different time points, then plasma and hepatic tissues were collected. Histological and clinical chemistry assays were performed to assess the progression of liver disease. MicroRNAs' differential expression was evaluated on pooled RNAs from tissues, and some miRNAs showing dysregulation were further analyzed at the individual level.
Cholesterol, low and high density lipoproteins, triglycerides and alanine aminotransferase increase was detected in HF mice. Gross anatomical examination revealed hepatomegaly in HF livers, and histological analysis highlighted different degrees and levels of steatosis, inflammatory infiltrate and fibrosis in HF and LF animals, demonstrating the progression from NAFLD through NASH. Macroscopic nodules, showing typical neoplastic features, were observed in 20% of HF diet fed mice. Fifteen miRNAs differentially expressed in HF with respect to LF hepatic tissues during the progression of liver damage, and in tumors with respect to HF non tumor liver specimens were identified. Among them, miR-340-5p, miR-484, miR-574-3p, miR-720, whose expression was never described in NAFLD, NASH and HCC tissues, and miR-125a-5p and miR-182, which showed early and significant dysregulation in the sequential hepatic damage process.
In this study, fifteen microRNAs which were modulated in hepatic tissues and in tumors during the transition NAFLD-NASH-HCC are reported. Besides some already described, new and early dysregulated miRNAs were identified. Functional analyses are needed to validate the results here obtained, and to better define the role of these molecules in the progression of the hepatic disease.</description><subject>Analysis</subject><subject>Animals</subject><subject>Carcinoma, Hepatocellular - genetics</subject><subject>Carcinoma, Hepatocellular - pathology</subject><subject>Cell Line, Tumor</subject><subject>Cholesterol - blood</subject><subject>Diet, High-Fat</subject><subject>Disease Models, Animal</subject><subject>Disease Progression</subject><subject>Gene Expression Regulation, Neoplastic</subject><subject>Hepatoma</subject><subject>Humans</subject><subject>Liver Neoplasms - genetics</subject><subject>Liver Neoplasms - pathology</subject><subject>Mice</subject><subject>MicroRNA</subject><subject>MicroRNAs - biosynthesis</subject><subject>MicroRNAs - genetics</subject><subject>Non-alcoholic Fatty Liver Disease - genetics</subject><subject>Non-alcoholic Fatty Liver Disease - pathology</subject><subject>Risk factors</subject><subject>Triglycerides - blood</subject><issn>1471-2407</issn><issn>1471-2407</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNptkkGL1DAcxYMo7u7oB_AiAUH0kN0kTZrUg1Cr66yMI-zuPaRp2kY6zdi0y863N8OMyxQkhITk997h_R8Abwi-JESmV4FQKTnChCOKsUDkGTgnTBBEGRbPT-5n4CKE3xgTIbF8Cc5oKqjEjJ2D9qczg79d59A-bgcbgvM91L3udsEF6HrYuqaFtR5h5eyIXF9NxlZwnV-vvqJ1frdEy6KA28E3R_EnGMap2sFoU3DxZXWV_oAbZ-wr8KLWXbCvj-cC3F9_uy-WaPXr-02Rr5BJUzEiLuMqs0pbXvIaS1ZTZjCtTMarhJYsoVldJpgKLWWdktJgonHG0xLT2iZlsgCfD7bbqdzYyth-HHSntoPb6GGnvHZq_tO7VjX-QTERE2QiGnw4Ggz-z2TDqDYuGNt1urd-CooIznCWyLgX4N0BbXRnletrHx3NHlc54yThUqY8Upf_oeKqbMzF97Z28X0m-DgTRGa0j2OjpxDUzd3tnH1_wrZWd2MbfDeNcRJhDpIDGKcdwmDrp0gIVvsyqUOZVCyT2pdJkah5e5rlk-Jfe5K_20TA0A</recordid><startdate>20160105</startdate><enddate>20160105</enddate><creator>Tessitore, Alessandra</creator><creator>Cicciarelli, Germana</creator><creator>Del Vecchio, Filippo</creator><creator>Gaggiano, Agata</creator><creator>Verzella, Daniela</creator><creator>Fischietti, Mariafausta</creator><creator>Mastroiaco, Valentina</creator><creator>Vetuschi, Antonella</creator><creator>Sferra, Roberta</creator><creator>Barnabei, Remo</creator><creator>Capece, Daria</creator><creator>Zazzeroni, Francesca</creator><creator>Alesse, Edoardo</creator><general>BioMed Central Ltd</general><general>BioMed Central</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>ISR</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20160105</creationdate><title>MicroRNA expression analysis in high fat diet-induced NAFLD-NASH-HCC progression: study on C57BL/6J mice</title><author>Tessitore, Alessandra ; Cicciarelli, Germana ; Del Vecchio, Filippo ; Gaggiano, Agata ; Verzella, Daniela ; Fischietti, Mariafausta ; Mastroiaco, Valentina ; Vetuschi, Antonella ; Sferra, Roberta ; Barnabei, Remo ; Capece, Daria ; Zazzeroni, Francesca ; Alesse, Edoardo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c667t-58585b9dae5b5f084f24c02dc95d32b4329fb3027a88f61bc01a0956b02fe3b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Analysis</topic><topic>Animals</topic><topic>Carcinoma, Hepatocellular - genetics</topic><topic>Carcinoma, Hepatocellular - pathology</topic><topic>Cell Line, Tumor</topic><topic>Cholesterol - blood</topic><topic>Diet, High-Fat</topic><topic>Disease Models, Animal</topic><topic>Disease Progression</topic><topic>Gene Expression Regulation, Neoplastic</topic><topic>Hepatoma</topic><topic>Humans</topic><topic>Liver Neoplasms - genetics</topic><topic>Liver Neoplasms - pathology</topic><topic>Mice</topic><topic>MicroRNA</topic><topic>MicroRNAs - biosynthesis</topic><topic>MicroRNAs - genetics</topic><topic>Non-alcoholic Fatty Liver Disease - genetics</topic><topic>Non-alcoholic Fatty Liver Disease - pathology</topic><topic>Risk factors</topic><topic>Triglycerides - blood</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Tessitore, Alessandra</creatorcontrib><creatorcontrib>Cicciarelli, Germana</creatorcontrib><creatorcontrib>Del Vecchio, Filippo</creatorcontrib><creatorcontrib>Gaggiano, Agata</creatorcontrib><creatorcontrib>Verzella, Daniela</creatorcontrib><creatorcontrib>Fischietti, Mariafausta</creatorcontrib><creatorcontrib>Mastroiaco, Valentina</creatorcontrib><creatorcontrib>Vetuschi, Antonella</creatorcontrib><creatorcontrib>Sferra, Roberta</creatorcontrib><creatorcontrib>Barnabei, Remo</creatorcontrib><creatorcontrib>Capece, Daria</creatorcontrib><creatorcontrib>Zazzeroni, Francesca</creatorcontrib><creatorcontrib>Alesse, Edoardo</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale In Context: Science</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>BMC cancer</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tessitore, Alessandra</au><au>Cicciarelli, Germana</au><au>Del Vecchio, Filippo</au><au>Gaggiano, Agata</au><au>Verzella, Daniela</au><au>Fischietti, Mariafausta</au><au>Mastroiaco, Valentina</au><au>Vetuschi, Antonella</au><au>Sferra, Roberta</au><au>Barnabei, Remo</au><au>Capece, Daria</au><au>Zazzeroni, Francesca</au><au>Alesse, Edoardo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>MicroRNA expression analysis in high fat diet-induced NAFLD-NASH-HCC progression: study on C57BL/6J mice</atitle><jtitle>BMC cancer</jtitle><addtitle>BMC Cancer</addtitle><date>2016-01-05</date><risdate>2016</risdate><volume>16</volume><issue>3</issue><spage>3</spage><epage>3</epage><pages>3-3</pages><artnum>3</artnum><issn>1471-2407</issn><eissn>1471-2407</eissn><abstract>Hepatocellular carcinoma (HCC) is the most common malignant tumor of the liver. Non-alcoholic fatty liver disease (NAFLD) is a frequent chronic liver disorder in developed countries. NAFLD can progress through the more severe non alcoholic steatohepatitis (NASH), cirrhosis and, lastly, HCC. Genetic and epigenetic alterations of coding genes as well as deregulation of microRNAs (miRNAs) activity play a role in HCC development. In this study, the C57BL/6J mouse model was long term high-fat (HF) or low-fat (LF) diet fed, in order to analyze molecular mechanisms responsible for the hepatic damage progression.
Mice were HF or LF diet fed for different time points, then plasma and hepatic tissues were collected. Histological and clinical chemistry assays were performed to assess the progression of liver disease. MicroRNAs' differential expression was evaluated on pooled RNAs from tissues, and some miRNAs showing dysregulation were further analyzed at the individual level.
Cholesterol, low and high density lipoproteins, triglycerides and alanine aminotransferase increase was detected in HF mice. Gross anatomical examination revealed hepatomegaly in HF livers, and histological analysis highlighted different degrees and levels of steatosis, inflammatory infiltrate and fibrosis in HF and LF animals, demonstrating the progression from NAFLD through NASH. Macroscopic nodules, showing typical neoplastic features, were observed in 20% of HF diet fed mice. Fifteen miRNAs differentially expressed in HF with respect to LF hepatic tissues during the progression of liver damage, and in tumors with respect to HF non tumor liver specimens were identified. Among them, miR-340-5p, miR-484, miR-574-3p, miR-720, whose expression was never described in NAFLD, NASH and HCC tissues, and miR-125a-5p and miR-182, which showed early and significant dysregulation in the sequential hepatic damage process.
In this study, fifteen microRNAs which were modulated in hepatic tissues and in tumors during the transition NAFLD-NASH-HCC are reported. Besides some already described, new and early dysregulated miRNAs were identified. Functional analyses are needed to validate the results here obtained, and to better define the role of these molecules in the progression of the hepatic disease.</abstract><cop>England</cop><pub>BioMed Central Ltd</pub><pmid>26728044</pmid><doi>10.1186/s12885-015-2007-1</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record> |
fulltext | fulltext |
identifier | ISSN: 1471-2407 |
ispartof | BMC cancer, 2016-01, Vol.16 (3), p.3-3, Article 3 |
issn | 1471-2407 1471-2407 |
language | eng |
recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_4700747 |
source | MEDLINE; DOAJ Directory of Open Access Journals; SpringerNature Journals; PubMed Central Open Access; Springer Nature OA Free Journals; EZB-FREE-00999 freely available EZB journals; PubMed Central |
subjects | Analysis Animals Carcinoma, Hepatocellular - genetics Carcinoma, Hepatocellular - pathology Cell Line, Tumor Cholesterol - blood Diet, High-Fat Disease Models, Animal Disease Progression Gene Expression Regulation, Neoplastic Hepatoma Humans Liver Neoplasms - genetics Liver Neoplasms - pathology Mice MicroRNA MicroRNAs - biosynthesis MicroRNAs - genetics Non-alcoholic Fatty Liver Disease - genetics Non-alcoholic Fatty Liver Disease - pathology Risk factors Triglycerides - blood |
title | MicroRNA expression analysis in high fat diet-induced NAFLD-NASH-HCC progression: study on C57BL/6J mice |
url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-25T14%3A54%3A14IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=MicroRNA%20expression%20analysis%20in%20high%20fat%20diet-induced%20NAFLD-NASH-HCC%20progression:%20study%20on%20C57BL/6J%20mice&rft.jtitle=BMC%20cancer&rft.au=Tessitore,%20Alessandra&rft.date=2016-01-05&rft.volume=16&rft.issue=3&rft.spage=3&rft.epage=3&rft.pages=3-3&rft.artnum=3&rft.issn=1471-2407&rft.eissn=1471-2407&rft_id=info:doi/10.1186/s12885-015-2007-1&rft_dat=%3Cgale_pubme%3EA451358865%3C/gale_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1754093809&rft_id=info:pmid/26728044&rft_galeid=A451358865&rfr_iscdi=true |