Cytotoxic and apoptotic effects of boron compounds on leukemia cell line
In this study we investigated the effects of boric acid and sodium tetraborate on an acute leukemia cell line and healthy human lymphocytes. We evaluated the effects of boric acid and sodium tetraborate on the HL-60 cell line and healthy human lymphocytes by using the methods of MTT, Neutral Red, AO...
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| Veröffentlicht in: | Cytotechnology (Dordrecht) 2016-01, Vol.68 (1), p.87-93 |
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| creator | Canturk, Zerrin Tunali, Yağmur Korkmaz, Seval Gulbaş, Zafer |
| description | In this study we investigated the effects of boric acid and sodium tetraborate on an acute leukemia cell line and healthy human lymphocytes. We evaluated the effects of boric acid and sodium tetraborate on the HL-60 cell line and healthy human lymphocytes by using the methods of MTT, Neutral Red, AO (flow cytometry) and transmission electron microscope. We found that there were dead cells at a concentration of 500 µM boric acid and sodium tetraborate (50 % and 40 %, respectively). An apoptotic effect was found at a concentration of 1,000 µM concentration in normal lymphocytes and HL-60 (acute leukemia cells) cells (2.5 % and 8.8 % respectively). We observed that boric acid at a concentration of 500 µM caused double nucleus and micronucleus formation in both HL-60 cells and lymphocytes. An expansion in mitochondrial dimension and deformation in cristas also appeared. Our findings suggest that boric acid is more effective than sodium tetraborate on the HL-60, and boric acid in particular showed a cytotoxic effect on HL-60 in comparison to healthy lymphocytes and it also affected the mitochondrial pathway. |
| doi_str_mv | 10.1007/s10616-014-9755-7 |
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We evaluated the effects of boric acid and sodium tetraborate on the HL-60 cell line and healthy human lymphocytes by using the methods of MTT, Neutral Red, AO (flow cytometry) and transmission electron microscope. We found that there were dead cells at a concentration of 500 µM boric acid and sodium tetraborate (50 % and 40 %, respectively). An apoptotic effect was found at a concentration of 1,000 µM concentration in normal lymphocytes and HL-60 (acute leukemia cells) cells (2.5 % and 8.8 % respectively). We observed that boric acid at a concentration of 500 µM caused double nucleus and micronucleus formation in both HL-60 cells and lymphocytes. An expansion in mitochondrial dimension and deformation in cristas also appeared. 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Our findings suggest that boric acid is more effective than sodium tetraborate on the HL-60, and boric acid in particular showed a cytotoxic effect on HL-60 in comparison to healthy lymphocytes and it also affected the mitochondrial pathway.</description><subject>Apoptosis</subject><subject>Biochemistry</subject><subject>Biomedicine</subject><subject>Biotechnology</subject><subject>Bone marrow</subject><subject>Boric acid</subject><subject>Boron</subject><subject>Cell cycle</subject><subject>Chemistry</subject><subject>Chemistry and Materials Science</subject><subject>Cytotoxicity</subject><subject>Electron microscopes</subject><subject>Flow cytometry</subject><subject>Leukemia</subject><subject>Lymphocytes</subject><subject>Original Research</subject><subject>Penicillin</subject><subject>Prostate cancer</subject><subject>Sodium</subject><subject>Sodium tetraborate</subject><issn>0920-9069</issn><issn>1573-0778</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>BENPR</sourceid><recordid>eNp1kU9P3DAQxS1UxC7bfoBeUKReuKSM_8eXStWKlkorcYGzZRybhiZ2sJOqfHu8WtgCEidrZn7zxk8Poc8YvmIAeZYxCCxqwKxWkvNaHqAl5pLWIGXzAS1BEagVCLVAxznfAYCSmB6hBeGYK07wEl2sH6Y4xX-drUxoKzPGsdSlct47O-Uq-uomphgqG4cxzqEtrVD1bv7jhs5U1vV91XfBfUSH3vTZfXp6V-j6x_nV-qLeXP78tf6-qa1gcqq94I0gLeNeKsUNUGabFqxRrG0bY60XhhJFqS8tAkS6xirqDDOSMs8F0BX6ttMd55vBtdaFKZlej6kbTHrQ0XT69SR0v_Vt_KuZUA2RpAicPgmkeD-7POmhy1sbJrg4Z40lp4wxQkVBv7xB7-KcQrGnicIN4QJTXii8o2yKOSfn95_BoLc56V1OuuSktzlpWXZOXrrYbzwHUwCyA3IZhVuX_p9-X_URVD6eQQ</recordid><startdate>20160101</startdate><enddate>20160101</enddate><creator>Canturk, Zerrin</creator><creator>Tunali, Yağmur</creator><creator>Korkmaz, Seval</creator><creator>Gulbaş, Zafer</creator><general>Springer Netherlands</general><general>Springer Nature B.V</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FE</scope><scope>8FH</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>LK8</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20160101</creationdate><title>Cytotoxic and apoptotic effects of boron compounds on leukemia cell line</title><author>Canturk, Zerrin ; Tunali, Yağmur ; Korkmaz, Seval ; Gulbaş, Zafer</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c647t-f65862d45f7995a034c8d0ca94dd8accf6a32933fca92027e8c93ea4a734f5603</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Apoptosis</topic><topic>Biochemistry</topic><topic>Biomedicine</topic><topic>Biotechnology</topic><topic>Bone marrow</topic><topic>Boric acid</topic><topic>Boron</topic><topic>Cell cycle</topic><topic>Chemistry</topic><topic>Chemistry and Materials Science</topic><topic>Cytotoxicity</topic><topic>Electron microscopes</topic><topic>Flow cytometry</topic><topic>Leukemia</topic><topic>Lymphocytes</topic><topic>Original Research</topic><topic>Penicillin</topic><topic>Prostate cancer</topic><topic>Sodium</topic><topic>Sodium tetraborate</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Canturk, Zerrin</creatorcontrib><creatorcontrib>Tunali, Yağmur</creatorcontrib><creatorcontrib>Korkmaz, Seval</creatorcontrib><creatorcontrib>Gulbaş, Zafer</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Biological Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Cytotechnology (Dordrecht)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Canturk, Zerrin</au><au>Tunali, Yağmur</au><au>Korkmaz, Seval</au><au>Gulbaş, Zafer</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cytotoxic and apoptotic effects of boron compounds on leukemia cell line</atitle><jtitle>Cytotechnology (Dordrecht)</jtitle><stitle>Cytotechnology</stitle><addtitle>Cytotechnology</addtitle><date>2016-01-01</date><risdate>2016</risdate><volume>68</volume><issue>1</issue><spage>87</spage><epage>93</epage><pages>87-93</pages><issn>0920-9069</issn><eissn>1573-0778</eissn><abstract>In this study we investigated the effects of boric acid and sodium tetraborate on an acute leukemia cell line and healthy human lymphocytes. We evaluated the effects of boric acid and sodium tetraborate on the HL-60 cell line and healthy human lymphocytes by using the methods of MTT, Neutral Red, AO (flow cytometry) and transmission electron microscope. We found that there were dead cells at a concentration of 500 µM boric acid and sodium tetraborate (50 % and 40 %, respectively). An apoptotic effect was found at a concentration of 1,000 µM concentration in normal lymphocytes and HL-60 (acute leukemia cells) cells (2.5 % and 8.8 % respectively). We observed that boric acid at a concentration of 500 µM caused double nucleus and micronucleus formation in both HL-60 cells and lymphocytes. An expansion in mitochondrial dimension and deformation in cristas also appeared. 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| subjects | Apoptosis Biochemistry Biomedicine Biotechnology Bone marrow Boric acid Boron Cell cycle Chemistry Chemistry and Materials Science Cytotoxicity Electron microscopes Flow cytometry Leukemia Lymphocytes Original Research Penicillin Prostate cancer Sodium Sodium tetraborate |
| title | Cytotoxic and apoptotic effects of boron compounds on leukemia cell line |
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