Purification and Refolding to Amyloid Fibrils of (His)6-tagged Recombinant Shadoo Protein Expressed as Inclusion Bodies in E. coli

The Escherichia coli expression system is a powerful tool for the production of recombinant eukaryotic proteins. We use it to produce Shadoo, a protein belonging to the prion family. A chromatographic method for the purification of (His)6-tagged recombinant Shadoo expressed as inclusion bodies is de...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Journal of Visualized Experiments 2015-12 (106), p.e53432-e53432
Hauptverfasser:	Li, Qiaojing, Richard, Charles-Adrien, Moudjou, Mohammed, Vidic, Jasmina
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Amyloid - biosynthesis Amyloid - chemistry Amyloid - genetics Amyloid - isolation & purification Animals Biology Chromatography, Gel - methods Escherichia coli - chemistry Escherichia coli - genetics Escherichia coli - metabolism Histidine - biosynthesis Histidine - chemistry Histidine - genetics Histidine - isolation & purification Inclusion Bodies - chemistry Inclusion Bodies - metabolism Mice Molecular Biology Molecular Sequence Data Nerve Tissue Proteins - biosynthesis Nerve Tissue Proteins - chemistry Nerve Tissue Proteins - genetics Nerve Tissue Proteins - isolation & purification Oligopeptides - biosynthesis Oligopeptides - chemistry Oligopeptides - genetics Oligopeptides - isolation & purification Protein Folding Protein Renaturation Recombinant Proteins - biosynthesis Recombinant Proteins - chemistry Recombinant Proteins - genetics Recombinant Proteins - isolation & purification Urea - chemistry
Online-Zugang:	Volltext bestellen
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Schreiben Sie den ersten Kommentar!