Expression of vascular endothelial growth factor and its role in oncogenesis of human gastric carcinoma
To establish the role of vascular endothelial growth factor (VEGF) in the oncogenesis of human gastric carcinoma more directly. The expression of VEGF and its receptor kinase-domain insert containing receptor (KDR) in human gastric cancer tissue were observed by immunohistochemical staining. VEGF le...
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| Veröffentlicht in: | World journal of gastroenterology : WJG 2001-08, Vol.7 (4), p.500-505 |
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| creator | Liu, D H Zhang, X Y Fan, D M Huang, Y X Zhang, J S Huang, W Q Zhang, Y Q Huang, Q S Ma, W Y Chai, Y B Jin, M |
| description | To establish the role of vascular endothelial growth factor (VEGF) in the oncogenesis of human gastric carcinoma more directly.
The expression of VEGF and its receptor kinase-domain insert containing receptor (KDR) in human gastric cancer tissue were observed by immunohistochemical staining. VEGF levels were manipulated in human gastric cancer cell using eukaryotic expression constructs designed to express the complete VEGF(165) complimentary DNA in either the sense or antisense orientation. The biological changes of the cells were observed in which VEGF was up-regulated or down-regulated.
VEGF-positive rate was 50%, and VEGF was mainly localized in the cytoplasm and membrane of the tumor cells, while KDR was mainly located in the membrane of vascular endothelial cells in gastric cancer tissues and peri-cancerous tissue. In 2 cases of 50 specimens, the gastric cancer cells expressed KDR, localized in both the cytoplasm and membrane. Introduction of VEGF(165) antisense into human gastric cancer cells (SGC-7901, immunofluorescence intensity, 31.6%)) resulted in a significant reduction in VEGF-specific messenger RNA and total and cell surface VEGF protein (immunofluorescence intensity, 8.9%) (P |
| doi_str_mv | 10.3748/wjg.v7.i4.500 |
| format | Article |
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The expression of VEGF and its receptor kinase-domain insert containing receptor (KDR) in human gastric cancer tissue were observed by immunohistochemical staining. VEGF levels were manipulated in human gastric cancer cell using eukaryotic expression constructs designed to express the complete VEGF(165) complimentary DNA in either the sense or antisense orientation. The biological changes of the cells were observed in which VEGF was up-regulated or down-regulated.
VEGF-positive rate was 50%, and VEGF was mainly localized in the cytoplasm and membrane of the tumor cells, while KDR was mainly located in the membrane of vascular endothelial cells in gastric cancer tissues and peri-cancerous tissue. In 2 cases of 50 specimens, the gastric cancer cells expressed KDR, localized in both the cytoplasm and membrane. Introduction of VEGF(165) antisense into human gastric cancer cells (SGC-7901, immunofluorescence intensity, 31.6%)) resulted in a significant reduction in VEGF-specific messenger RNA and total and cell surface VEGF protein (immunofluorescence intensity, 8.9%) (P<0.05). Conversely, stable integration of VEGF(165) in the sense orientation resulted in an increase in cellular and cell surface VEGF (immunofluorescence intensity, 75.4%) (P<0.05). Lowered VEGF levels were associated with a marked decrease in the growth of nude mouse xenografted tumor (at 33 days postimplantation, tumor volume: 345.40 +/- 136.31 mm3)(P<0.05 vs control SGC-7901 group: 1534.40 +/- 362.88 mm3), whereas up-regulation of VEGF resulted in increased xenografted tumor size (at 33 days postimplantation, tumor volume: 2350.50 +/- 637.70 mm3) (P<0.05 vs control SGC-7901 group).
This study provides direct evidence that VEGF plays an important role in the oncogenesis of human gastric cancer.</description><identifier>ISSN: 1007-9327</identifier><identifier>EISSN: 2219-2840</identifier><identifier>DOI: 10.3748/wjg.v7.i4.500</identifier><identifier>PMID: 11819817</identifier><language>eng</language><publisher>United States: lnstitute of Digestive Disease, Xijing Hospital%Department of Gastroenterology, Tangdu Hospital%Department of Histology and Embryology%Department of Microbiology%Department of Biochemistry, Fourth Military Medical University, Xi an 710033,Shaanxi Province, China</publisher><subject>Adult ; Aged ; Animals ; Cell Division ; Cloning, Molecular ; DNA, Antisense ; DNA, Complementary ; Endothelial Growth Factors - genetics ; Endothelium, Vascular - physiology ; Female ; Gene Expression Regulation, Neoplastic ; Humans ; Lymphokines - genetics ; Male ; Mice ; Mice, Nude ; Middle Aged ; Neovascularization, Pathologic - pathology ; Neovascularization, Pathologic - physiopathology ; Original Research ; Receptor Protein-Tyrosine Kinases - genetics ; Receptors, Growth Factor - genetics ; Receptors, Vascular Endothelial Growth Factor ; Stomach Neoplasms - pathology ; Stomach Neoplasms - physiopathology ; Transfection ; Tumor Cells, Cultured ; Vascular Endothelial Growth Factor A ; Vascular Endothelial Growth Factors</subject><ispartof>World journal of gastroenterology : WJG, 2001-08, Vol.7 (4), p.500-505</ispartof><rights>Copyright © Wanfang Data Co. Ltd. All Rights Reserved.</rights><rights>The Author(s) 2001. Published by Baishideng Publishing Group Inc. All rights reserved. 2001</rights><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c412t-d425b523c0aa99754e40616264996d91d08004ec06801660c8fdd6a0a6ea94113</citedby><cites>FETCH-LOGICAL-c412t-d425b523c0aa99754e40616264996d91d08004ec06801660c8fdd6a0a6ea94113</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.wanfangdata.com.cn/images/PeriodicalImages/wjg/wjg.jpg</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4688661/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4688661/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,27923,27924,53790,53792</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11819817$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Liu, D H</creatorcontrib><creatorcontrib>Zhang, X Y</creatorcontrib><creatorcontrib>Fan, D M</creatorcontrib><creatorcontrib>Huang, Y X</creatorcontrib><creatorcontrib>Zhang, J S</creatorcontrib><creatorcontrib>Huang, W Q</creatorcontrib><creatorcontrib>Zhang, Y Q</creatorcontrib><creatorcontrib>Huang, Q S</creatorcontrib><creatorcontrib>Ma, W Y</creatorcontrib><creatorcontrib>Chai, Y B</creatorcontrib><creatorcontrib>Jin, M</creatorcontrib><title>Expression of vascular endothelial growth factor and its role in oncogenesis of human gastric carcinoma</title><title>World journal of gastroenterology : WJG</title><addtitle>World J Gastroenterol</addtitle><description>To establish the role of vascular endothelial growth factor (VEGF) in the oncogenesis of human gastric carcinoma more directly.
The expression of VEGF and its receptor kinase-domain insert containing receptor (KDR) in human gastric cancer tissue were observed by immunohistochemical staining. VEGF levels were manipulated in human gastric cancer cell using eukaryotic expression constructs designed to express the complete VEGF(165) complimentary DNA in either the sense or antisense orientation. The biological changes of the cells were observed in which VEGF was up-regulated or down-regulated.
VEGF-positive rate was 50%, and VEGF was mainly localized in the cytoplasm and membrane of the tumor cells, while KDR was mainly located in the membrane of vascular endothelial cells in gastric cancer tissues and peri-cancerous tissue. In 2 cases of 50 specimens, the gastric cancer cells expressed KDR, localized in both the cytoplasm and membrane. Introduction of VEGF(165) antisense into human gastric cancer cells (SGC-7901, immunofluorescence intensity, 31.6%)) resulted in a significant reduction in VEGF-specific messenger RNA and total and cell surface VEGF protein (immunofluorescence intensity, 8.9%) (P<0.05). Conversely, stable integration of VEGF(165) in the sense orientation resulted in an increase in cellular and cell surface VEGF (immunofluorescence intensity, 75.4%) (P<0.05). Lowered VEGF levels were associated with a marked decrease in the growth of nude mouse xenografted tumor (at 33 days postimplantation, tumor volume: 345.40 +/- 136.31 mm3)(P<0.05 vs control SGC-7901 group: 1534.40 +/- 362.88 mm3), whereas up-regulation of VEGF resulted in increased xenografted tumor size (at 33 days postimplantation, tumor volume: 2350.50 +/- 637.70 mm3) (P<0.05 vs control SGC-7901 group).
This study provides direct evidence that VEGF plays an important role in the oncogenesis of human gastric cancer.</description><subject>Adult</subject><subject>Aged</subject><subject>Animals</subject><subject>Cell Division</subject><subject>Cloning, Molecular</subject><subject>DNA, Antisense</subject><subject>DNA, Complementary</subject><subject>Endothelial Growth Factors - genetics</subject><subject>Endothelium, Vascular - physiology</subject><subject>Female</subject><subject>Gene Expression Regulation, Neoplastic</subject><subject>Humans</subject><subject>Lymphokines - genetics</subject><subject>Male</subject><subject>Mice</subject><subject>Mice, Nude</subject><subject>Middle Aged</subject><subject>Neovascularization, Pathologic - pathology</subject><subject>Neovascularization, Pathologic - physiopathology</subject><subject>Original Research</subject><subject>Receptor Protein-Tyrosine Kinases - genetics</subject><subject>Receptors, Growth Factor - genetics</subject><subject>Receptors, Vascular Endothelial Growth Factor</subject><subject>Stomach Neoplasms - pathology</subject><subject>Stomach Neoplasms - physiopathology</subject><subject>Transfection</subject><subject>Tumor Cells, Cultured</subject><subject>Vascular Endothelial Growth Factor A</subject><subject>Vascular Endothelial Growth Factors</subject><issn>1007-9327</issn><issn>2219-2840</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVkUtv3CAURlHUKJk8ltlGLKruPLlgjGFTqYrSplKkbJI1usHYw8iGKdgzyb-vRzPqY8Xinnv44CPkhsGyrIW626275bZeerGsAE7IgnOmC64EfCILBlAXuuT1ObnIeQ3Ay7LiZ-ScMcW0YvWCdA_vm-Ry9jHQ2NItZjv1mKgLTRxXrvfY0y7F3biiLdoxJoqhoX7MNMXeUT9vBRs7F1z2eW9YTQMG2mEek7fUYrI-xAGvyGmLfXbXx_OSvH5_eLl_LJ6ef_y8__ZUWMH4WDSCV28VLy0gal1XwgmQTHIptJaNZg0oAOEsSAVMSrCqbRqJgNKhFoyVl-TrwbuZ3gbXWBfGhL3ZJD9g-jARvfl_EvzKdHFrhFRKyr3g80Gww9Bi6Mw6TinMkc380xyAgQDQM_bleE-KvyaXRzP4bF3fY3BxyqZmJfA5zwwWB9CmmHNy7Z8sDMy-wb3XbGvjhZkbnPnbfx_wlz5WVv4GY3iZRg</recordid><startdate>20010801</startdate><enddate>20010801</enddate><creator>Liu, D H</creator><creator>Zhang, X Y</creator><creator>Fan, D M</creator><creator>Huang, Y X</creator><creator>Zhang, J S</creator><creator>Huang, W Q</creator><creator>Zhang, Y Q</creator><creator>Huang, Q S</creator><creator>Ma, W Y</creator><creator>Chai, Y B</creator><creator>Jin, M</creator><general>lnstitute of Digestive Disease, Xijing Hospital%Department of Gastroenterology, Tangdu Hospital%Department of Histology and Embryology%Department of Microbiology%Department of Biochemistry, Fourth Military Medical University, Xi an 710033,Shaanxi Province, China</general><general>Baishideng Publishing Group Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>2B.</scope><scope>4A8</scope><scope>92I</scope><scope>93N</scope><scope>PSX</scope><scope>TCJ</scope><scope>5PM</scope></search><sort><creationdate>20010801</creationdate><title>Expression of vascular endothelial growth factor and its role in oncogenesis of human gastric carcinoma</title><author>Liu, D H ; Zhang, X Y ; Fan, D M ; Huang, Y X ; Zhang, J S ; Huang, W Q ; Zhang, Y Q ; Huang, Q S ; Ma, W Y ; Chai, Y B ; Jin, M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c412t-d425b523c0aa99754e40616264996d91d08004ec06801660c8fdd6a0a6ea94113</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Adult</topic><topic>Aged</topic><topic>Animals</topic><topic>Cell Division</topic><topic>Cloning, Molecular</topic><topic>DNA, Antisense</topic><topic>DNA, Complementary</topic><topic>Endothelial Growth Factors - genetics</topic><topic>Endothelium, Vascular - physiology</topic><topic>Female</topic><topic>Gene Expression Regulation, Neoplastic</topic><topic>Humans</topic><topic>Lymphokines - genetics</topic><topic>Male</topic><topic>Mice</topic><topic>Mice, Nude</topic><topic>Middle Aged</topic><topic>Neovascularization, Pathologic - pathology</topic><topic>Neovascularization, Pathologic - physiopathology</topic><topic>Original Research</topic><topic>Receptor Protein-Tyrosine Kinases - genetics</topic><topic>Receptors, Growth Factor - genetics</topic><topic>Receptors, Vascular Endothelial Growth Factor</topic><topic>Stomach Neoplasms - pathology</topic><topic>Stomach Neoplasms - physiopathology</topic><topic>Transfection</topic><topic>Tumor Cells, Cultured</topic><topic>Vascular Endothelial Growth Factor A</topic><topic>Vascular Endothelial Growth Factors</topic><toplevel>online_resources</toplevel><creatorcontrib>Liu, D H</creatorcontrib><creatorcontrib>Zhang, X Y</creatorcontrib><creatorcontrib>Fan, D M</creatorcontrib><creatorcontrib>Huang, Y X</creatorcontrib><creatorcontrib>Zhang, J S</creatorcontrib><creatorcontrib>Huang, W Q</creatorcontrib><creatorcontrib>Zhang, Y Q</creatorcontrib><creatorcontrib>Huang, Q S</creatorcontrib><creatorcontrib>Ma, W Y</creatorcontrib><creatorcontrib>Chai, Y B</creatorcontrib><creatorcontrib>Jin, M</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Wanfang Data Journals - Hong Kong</collection><collection>WANFANG Data Centre</collection><collection>Wanfang Data Journals</collection><collection>万方数据期刊 - 香港版</collection><collection>China Online Journals (COJ)</collection><collection>China Online Journals (COJ)</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>World journal of gastroenterology : WJG</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Liu, D H</au><au>Zhang, X Y</au><au>Fan, D M</au><au>Huang, Y X</au><au>Zhang, J S</au><au>Huang, W Q</au><au>Zhang, Y Q</au><au>Huang, Q S</au><au>Ma, W Y</au><au>Chai, Y B</au><au>Jin, M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Expression of vascular endothelial growth factor and its role in oncogenesis of human gastric carcinoma</atitle><jtitle>World journal of gastroenterology : WJG</jtitle><addtitle>World J Gastroenterol</addtitle><date>2001-08-01</date><risdate>2001</risdate><volume>7</volume><issue>4</issue><spage>500</spage><epage>505</epage><pages>500-505</pages><issn>1007-9327</issn><eissn>2219-2840</eissn><abstract>To establish the role of vascular endothelial growth factor (VEGF) in the oncogenesis of human gastric carcinoma more directly.
The expression of VEGF and its receptor kinase-domain insert containing receptor (KDR) in human gastric cancer tissue were observed by immunohistochemical staining. VEGF levels were manipulated in human gastric cancer cell using eukaryotic expression constructs designed to express the complete VEGF(165) complimentary DNA in either the sense or antisense orientation. The biological changes of the cells were observed in which VEGF was up-regulated or down-regulated.
VEGF-positive rate was 50%, and VEGF was mainly localized in the cytoplasm and membrane of the tumor cells, while KDR was mainly located in the membrane of vascular endothelial cells in gastric cancer tissues and peri-cancerous tissue. In 2 cases of 50 specimens, the gastric cancer cells expressed KDR, localized in both the cytoplasm and membrane. Introduction of VEGF(165) antisense into human gastric cancer cells (SGC-7901, immunofluorescence intensity, 31.6%)) resulted in a significant reduction in VEGF-specific messenger RNA and total and cell surface VEGF protein (immunofluorescence intensity, 8.9%) (P<0.05). Conversely, stable integration of VEGF(165) in the sense orientation resulted in an increase in cellular and cell surface VEGF (immunofluorescence intensity, 75.4%) (P<0.05). Lowered VEGF levels were associated with a marked decrease in the growth of nude mouse xenografted tumor (at 33 days postimplantation, tumor volume: 345.40 +/- 136.31 mm3)(P<0.05 vs control SGC-7901 group: 1534.40 +/- 362.88 mm3), whereas up-regulation of VEGF resulted in increased xenografted tumor size (at 33 days postimplantation, tumor volume: 2350.50 +/- 637.70 mm3) (P<0.05 vs control SGC-7901 group).
This study provides direct evidence that VEGF plays an important role in the oncogenesis of human gastric cancer.</abstract><cop>United States</cop><pub>lnstitute of Digestive Disease, Xijing Hospital%Department of Gastroenterology, Tangdu Hospital%Department of Histology and Embryology%Department of Microbiology%Department of Biochemistry, Fourth Military Medical University, Xi an 710033,Shaanxi Province, China</pub><pmid>11819817</pmid><doi>10.3748/wjg.v7.i4.500</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Adult Aged Animals Cell Division Cloning, Molecular DNA, Antisense DNA, Complementary Endothelial Growth Factors - genetics Endothelium, Vascular - physiology Female Gene Expression Regulation, Neoplastic Humans Lymphokines - genetics Male Mice Mice, Nude Middle Aged Neovascularization, Pathologic - pathology Neovascularization, Pathologic - physiopathology Original Research Receptor Protein-Tyrosine Kinases - genetics Receptors, Growth Factor - genetics Receptors, Vascular Endothelial Growth Factor Stomach Neoplasms - pathology Stomach Neoplasms - physiopathology Transfection Tumor Cells, Cultured Vascular Endothelial Growth Factor A Vascular Endothelial Growth Factors |
| title | Expression of vascular endothelial growth factor and its role in oncogenesis of human gastric carcinoma |
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