Effect of ganglioside GT1b on the in vitro maturation of porcine oocytes and embryonic development
Ganglioside is an acidic glycosphingolipid with sialic acids residues. This study was performed to investigate the effect and mechanism of ganglioside GT1b in porcine oocytes in the process of in vitro maturation (IVM) and preimplantation development. Metaphase II (MII) rates were significantly (P &...
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| Veröffentlicht in: | Journal of Reproduction and Development 2015, Vol.61(6), pp.549-557 |
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| creator | HWANG, Seon-Ung JEON, Yubyeol YOON, Junchul David CAI, Lian KIM, Eunhye YOO, Hyunju KIM, Kyu-Jun PARK, Kyu Mi JIN, Minghui KIM, Hyunggee HYUN, Sang-Hwan |
| description | Ganglioside is an acidic glycosphingolipid with sialic acids residues. This study was performed to investigate the effect and mechanism of ganglioside GT1b in porcine oocytes in the process of in vitro maturation (IVM) and preimplantation development. Metaphase II (MII) rates were significantly (P < 0.05) different between the control group and the 5 nM GT1b treatment group. Intracellular glutathione (GSH) levels in oocytes matured with 5 nM and 20 nM and GT1b decreased significantly (P < 0.05). The 10 nM group showed a significant (P < 0.05) decrease in intracellular reactive oxygen species (ROS) levels compared with the control group. Subsequently, the level of intracellular Ca2+ in oocytes treated with different concentrations of GT1b was measured. Intracellular Ca2+ was significantly (P < 0.05) increased with a higher concentration of GT1b in a dose-dependent manner. Real-time PCR was performed and showed that the expression of bradykinin 2 receptor (B2R) and calcium/calmodulin-dependent protein kinase II delta (CaMKIIδ) in cumulus cells was significantly (P < 0.05) decreased in the 20 nM GT1b treatment group. Treatment with 5 nM GT1b significantly (P < 0.05) decreased the expression of CaMKIIδ. In oocytes, treatment with 5 nM GT1b significantly (P < 0.05) decreased CaMKIIγ and POU5F1 (POU domain, class 5, transcription factor 1). However, treatment with 20 nM GT1b significantly (P < 0.05) increased the expression of POU5F1. Finally, embryonic developmental data showed no significant differences in the two experiments (parthenogenesis and in vitro fertilization). In conclusion, the results of the present study indicated that GT1b plays an important role in increasing the nuclear maturation rate and decreasing the intracellular ROS levels during IVM. However, GT1b inhibited maturation of the cytoplasm by maintaining intracellular Ca2+ in the process of oocyte maturation regardless of the cell cycle stage. Therefore, GT1b is thought to act on another mechanism that controls intracellular Ca2+. |
| doi_str_mv | 10.1262/jrd.2015-049 |
| format | Article |
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This study was performed to investigate the effect and mechanism of ganglioside GT1b in porcine oocytes in the process of in vitro maturation (IVM) and preimplantation development. Metaphase II (MII) rates were significantly (P < 0.05) different between the control group and the 5 nM GT1b treatment group. Intracellular glutathione (GSH) levels in oocytes matured with 5 nM and 20 nM and GT1b decreased significantly (P < 0.05). The 10 nM group showed a significant (P < 0.05) decrease in intracellular reactive oxygen species (ROS) levels compared with the control group. Subsequently, the level of intracellular Ca2+ in oocytes treated with different concentrations of GT1b was measured. Intracellular Ca2+ was significantly (P < 0.05) increased with a higher concentration of GT1b in a dose-dependent manner. Real-time PCR was performed and showed that the expression of bradykinin 2 receptor (B2R) and calcium/calmodulin-dependent protein kinase II delta (CaMKIIδ) in cumulus cells was significantly (P < 0.05) decreased in the 20 nM GT1b treatment group. Treatment with 5 nM GT1b significantly (P < 0.05) decreased the expression of CaMKIIδ. In oocytes, treatment with 5 nM GT1b significantly (P < 0.05) decreased CaMKIIγ and POU5F1 (POU domain, class 5, transcription factor 1). However, treatment with 20 nM GT1b significantly (P < 0.05) increased the expression of POU5F1. Finally, embryonic developmental data showed no significant differences in the two experiments (parthenogenesis and in vitro fertilization). In conclusion, the results of the present study indicated that GT1b plays an important role in increasing the nuclear maturation rate and decreasing the intracellular ROS levels during IVM. However, GT1b inhibited maturation of the cytoplasm by maintaining intracellular Ca2+ in the process of oocyte maturation regardless of the cell cycle stage. Therefore, GT1b is thought to act on another mechanism that controls intracellular Ca2+.]]></description><identifier>ISSN: 0916-8818</identifier><identifier>EISSN: 1348-4400</identifier><identifier>DOI: 10.1262/jrd.2015-049</identifier><identifier>PMID: 26370787</identifier><language>eng</language><publisher>Japan: THE SOCIETY FOR REPRODUCTION AND DEVELOPMENT</publisher><subject>Animals ; Calcium - analysis ; Embryonic development ; Embryonic Development - physiology ; Female ; Fertilization in Vitro ; Ganglioside GT1b ; Gangliosides - physiology ; Glutathione - analysis ; In vitro maturation (IVM) ; In Vitro Oocyte Maturation Techniques ; Intracellular calcium ; Original ; Porcine ; Reactive Oxygen Species ; Real-Time Polymerase Chain Reaction ; Receptor, Bradykinin B2 - analysis ; RNA, Messenger - analysis ; Swine - physiology</subject><ispartof>Journal of Reproduction and Development, 2015, Vol.61(6), pp.549-557</ispartof><rights>2015 Society for Reproduction and Development</rights><rights>2015 Society for Reproduction and Development 2015</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5329-1154ce2969137f4b3c5e9807558bed599a7d4e4c76c00bfa1f196ffebbc5c0bc3</citedby><cites>FETCH-LOGICAL-c5329-1154ce2969137f4b3c5e9807558bed599a7d4e4c76c00bfa1f196ffebbc5c0bc3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4685221/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4685221/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,1881,4022,27922,27923,27924,53790,53792</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26370787$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>HWANG, Seon-Ung</creatorcontrib><creatorcontrib>JEON, Yubyeol</creatorcontrib><creatorcontrib>YOON, Junchul David</creatorcontrib><creatorcontrib>CAI, Lian</creatorcontrib><creatorcontrib>KIM, Eunhye</creatorcontrib><creatorcontrib>YOO, Hyunju</creatorcontrib><creatorcontrib>KIM, Kyu-Jun</creatorcontrib><creatorcontrib>PARK, Kyu Mi</creatorcontrib><creatorcontrib>JIN, Minghui</creatorcontrib><creatorcontrib>KIM, Hyunggee</creatorcontrib><creatorcontrib>HYUN, Sang-Hwan</creatorcontrib><title>Effect of ganglioside GT1b on the in vitro maturation of porcine oocytes and embryonic development</title><title>Journal of Reproduction and Development</title><addtitle>J. Reprod. Dev.</addtitle><description><![CDATA[Ganglioside is an acidic glycosphingolipid with sialic acids residues. This study was performed to investigate the effect and mechanism of ganglioside GT1b in porcine oocytes in the process of in vitro maturation (IVM) and preimplantation development. Metaphase II (MII) rates were significantly (P < 0.05) different between the control group and the 5 nM GT1b treatment group. Intracellular glutathione (GSH) levels in oocytes matured with 5 nM and 20 nM and GT1b decreased significantly (P < 0.05). The 10 nM group showed a significant (P < 0.05) decrease in intracellular reactive oxygen species (ROS) levels compared with the control group. Subsequently, the level of intracellular Ca2+ in oocytes treated with different concentrations of GT1b was measured. Intracellular Ca2+ was significantly (P < 0.05) increased with a higher concentration of GT1b in a dose-dependent manner. Real-time PCR was performed and showed that the expression of bradykinin 2 receptor (B2R) and calcium/calmodulin-dependent protein kinase II delta (CaMKIIδ) in cumulus cells was significantly (P < 0.05) decreased in the 20 nM GT1b treatment group. Treatment with 5 nM GT1b significantly (P < 0.05) decreased the expression of CaMKIIδ. In oocytes, treatment with 5 nM GT1b significantly (P < 0.05) decreased CaMKIIγ and POU5F1 (POU domain, class 5, transcription factor 1). However, treatment with 20 nM GT1b significantly (P < 0.05) increased the expression of POU5F1. Finally, embryonic developmental data showed no significant differences in the two experiments (parthenogenesis and in vitro fertilization). In conclusion, the results of the present study indicated that GT1b plays an important role in increasing the nuclear maturation rate and decreasing the intracellular ROS levels during IVM. However, GT1b inhibited maturation of the cytoplasm by maintaining intracellular Ca2+ in the process of oocyte maturation regardless of the cell cycle stage. Therefore, GT1b is thought to act on another mechanism that controls intracellular Ca2+.]]></description><subject>Animals</subject><subject>Calcium - analysis</subject><subject>Embryonic development</subject><subject>Embryonic Development - physiology</subject><subject>Female</subject><subject>Fertilization in Vitro</subject><subject>Ganglioside GT1b</subject><subject>Gangliosides - physiology</subject><subject>Glutathione - analysis</subject><subject>In vitro maturation (IVM)</subject><subject>In Vitro Oocyte Maturation Techniques</subject><subject>Intracellular calcium</subject><subject>Original</subject><subject>Porcine</subject><subject>Reactive Oxygen Species</subject><subject>Real-Time Polymerase Chain Reaction</subject><subject>Receptor, Bradykinin B2 - analysis</subject><subject>RNA, Messenger - analysis</subject><subject>Swine - physiology</subject><issn>0916-8818</issn><issn>1348-4400</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVkMFuGyEQhlHUqnHd3nKueIBsCiywy6VSazlppUi9uGcE7KyNtQsWiy357YPj2EovjMR888_oQ-iOkgfKJPu-Td0DI1RUhKsbNKM1byvOCfmAZkRRWbUtbW_R52naElIzIfkndMtk3ZCmbWbILvseXMaxx2sT1oOPk-8AP62oxTHgvAHsAz74nCIeTd4nk335L_guJucD4BjdMcOETegwjDYdY_AOd3CAIe5GCPkL-tibYYKvb3WO_j0uV4vf1fPfpz-Ln8-VEzVTFaWCO2BKKlo3Pbe1E6Ba0gjRWuiEUqbpOHDXSEeI7Q3tqZLleGudcMS6eo5-nHN3eztC58rqZAa9S3406aij8fr_TvAbvY4HzWUrGKMl4P4c4FKcpgT9dZYSfXKti2t9cq2L64J_e7_vCl_kFuDXGdhO2azhCpiUvRvgNU1SLU_PJfXadBuTNIT6BZzGlOI</recordid><startdate>2015</startdate><enddate>2015</enddate><creator>HWANG, Seon-Ung</creator><creator>JEON, Yubyeol</creator><creator>YOON, Junchul David</creator><creator>CAI, Lian</creator><creator>KIM, Eunhye</creator><creator>YOO, Hyunju</creator><creator>KIM, Kyu-Jun</creator><creator>PARK, Kyu Mi</creator><creator>JIN, Minghui</creator><creator>KIM, Hyunggee</creator><creator>HYUN, Sang-Hwan</creator><general>THE SOCIETY FOR REPRODUCTION AND DEVELOPMENT</general><general>The Society for Reproduction and Development</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>5PM</scope></search><sort><creationdate>2015</creationdate><title>Effect of ganglioside GT1b on the in vitro maturation of porcine oocytes and embryonic development</title><author>HWANG, Seon-Ung ; JEON, Yubyeol ; YOON, Junchul David ; CAI, Lian ; KIM, Eunhye ; YOO, Hyunju ; KIM, Kyu-Jun ; PARK, Kyu Mi ; JIN, Minghui ; KIM, Hyunggee ; HYUN, Sang-Hwan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5329-1154ce2969137f4b3c5e9807558bed599a7d4e4c76c00bfa1f196ffebbc5c0bc3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Animals</topic><topic>Calcium - analysis</topic><topic>Embryonic development</topic><topic>Embryonic Development - physiology</topic><topic>Female</topic><topic>Fertilization in Vitro</topic><topic>Ganglioside GT1b</topic><topic>Gangliosides - physiology</topic><topic>Glutathione - analysis</topic><topic>In vitro maturation (IVM)</topic><topic>In Vitro Oocyte Maturation Techniques</topic><topic>Intracellular calcium</topic><topic>Original</topic><topic>Porcine</topic><topic>Reactive Oxygen Species</topic><topic>Real-Time Polymerase Chain Reaction</topic><topic>Receptor, Bradykinin B2 - analysis</topic><topic>RNA, Messenger - analysis</topic><topic>Swine - physiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>HWANG, Seon-Ung</creatorcontrib><creatorcontrib>JEON, Yubyeol</creatorcontrib><creatorcontrib>YOON, Junchul David</creatorcontrib><creatorcontrib>CAI, Lian</creatorcontrib><creatorcontrib>KIM, Eunhye</creatorcontrib><creatorcontrib>YOO, Hyunju</creatorcontrib><creatorcontrib>KIM, Kyu-Jun</creatorcontrib><creatorcontrib>PARK, Kyu Mi</creatorcontrib><creatorcontrib>JIN, Minghui</creatorcontrib><creatorcontrib>KIM, Hyunggee</creatorcontrib><creatorcontrib>HYUN, Sang-Hwan</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of Reproduction and Development</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>HWANG, Seon-Ung</au><au>JEON, Yubyeol</au><au>YOON, Junchul David</au><au>CAI, Lian</au><au>KIM, Eunhye</au><au>YOO, Hyunju</au><au>KIM, Kyu-Jun</au><au>PARK, Kyu Mi</au><au>JIN, Minghui</au><au>KIM, Hyunggee</au><au>HYUN, Sang-Hwan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of ganglioside GT1b on the in vitro maturation of porcine oocytes and embryonic development</atitle><jtitle>Journal of Reproduction and Development</jtitle><addtitle>J. Reprod. Dev.</addtitle><date>2015</date><risdate>2015</risdate><volume>61</volume><issue>6</issue><spage>549</spage><epage>557</epage><pages>549-557</pages><issn>0916-8818</issn><eissn>1348-4400</eissn><abstract><![CDATA[Ganglioside is an acidic glycosphingolipid with sialic acids residues. This study was performed to investigate the effect and mechanism of ganglioside GT1b in porcine oocytes in the process of in vitro maturation (IVM) and preimplantation development. Metaphase II (MII) rates were significantly (P < 0.05) different between the control group and the 5 nM GT1b treatment group. Intracellular glutathione (GSH) levels in oocytes matured with 5 nM and 20 nM and GT1b decreased significantly (P < 0.05). The 10 nM group showed a significant (P < 0.05) decrease in intracellular reactive oxygen species (ROS) levels compared with the control group. Subsequently, the level of intracellular Ca2+ in oocytes treated with different concentrations of GT1b was measured. Intracellular Ca2+ was significantly (P < 0.05) increased with a higher concentration of GT1b in a dose-dependent manner. Real-time PCR was performed and showed that the expression of bradykinin 2 receptor (B2R) and calcium/calmodulin-dependent protein kinase II delta (CaMKIIδ) in cumulus cells was significantly (P < 0.05) decreased in the 20 nM GT1b treatment group. Treatment with 5 nM GT1b significantly (P < 0.05) decreased the expression of CaMKIIδ. In oocytes, treatment with 5 nM GT1b significantly (P < 0.05) decreased CaMKIIγ and POU5F1 (POU domain, class 5, transcription factor 1). However, treatment with 20 nM GT1b significantly (P < 0.05) increased the expression of POU5F1. Finally, embryonic developmental data showed no significant differences in the two experiments (parthenogenesis and in vitro fertilization). In conclusion, the results of the present study indicated that GT1b plays an important role in increasing the nuclear maturation rate and decreasing the intracellular ROS levels during IVM. However, GT1b inhibited maturation of the cytoplasm by maintaining intracellular Ca2+ in the process of oocyte maturation regardless of the cell cycle stage. Therefore, GT1b is thought to act on another mechanism that controls intracellular Ca2+.]]></abstract><cop>Japan</cop><pub>THE SOCIETY FOR REPRODUCTION AND DEVELOPMENT</pub><pmid>26370787</pmid><doi>10.1262/jrd.2015-049</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Animals Calcium - analysis Embryonic development Embryonic Development - physiology Female Fertilization in Vitro Ganglioside GT1b Gangliosides - physiology Glutathione - analysis In vitro maturation (IVM) In Vitro Oocyte Maturation Techniques Intracellular calcium Original Porcine Reactive Oxygen Species Real-Time Polymerase Chain Reaction Receptor, Bradykinin B2 - analysis RNA, Messenger - analysis Swine - physiology |
| title | Effect of ganglioside GT1b on the in vitro maturation of porcine oocytes and embryonic development |
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