Serum lipidomic profiling as a useful tool for screening potential biomarkers of hepatitis B-related hepatocellular carcinoma by ultraperformance liquid chromatography-mass spectrometry
Chronic hepatitis B (CHB) virus infection is a major cause of hepatocellular carcinoma (HCC), as late diagnosis is the main factor for the poor survival of patients. There is an urgent need for accurate biomarkers for early diagnosis of HCC. The aim of the study was to explore the serum lipidome pro...
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| Veröffentlicht in: | BMC cancer 2015-12, Vol.15 (983), p.985-985, Article 985 |
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| creator | Passos-Castilho, Ana Maria Carvalho, Valdemir Melechco Cardozo, Karina Helena Morais Kikuchi, Luciana Chagas, Aline Lopes Gomes-Gouvêa, Michele Soares Malta, Fernanda de Seixas-Santos Nastri, Ana Catharina Pinho, João Renato Rebello Carrilho, Flair José Granato, Celso Francisco Hernandes |
| description | Chronic hepatitis B (CHB) virus infection is a major cause of hepatocellular carcinoma (HCC), as late diagnosis is the main factor for the poor survival of patients. There is an urgent need for accurate biomarkers for early diagnosis of HCC. The aim of the study was to explore the serum lipidome profiles of hepatitis B-related HCC to identify potential diagnostic biomarkers.
An ultraperformance liquid chromatography mass spectrometry (UPLC-MS) lipidomic method was used to characterize serum profiles from HCC (n = 32), liver cirrhosis (LC) (n = 30), CHB (n = 25), and healthy subjects (n = 34). Patients were diagnosed by clinical laboratory and imaging evidence and all presented with CHB while healthy controls had normal liver function and no infectious diseases.
The UPLC-MS-based serum lipidomic profile provided more accurate diagnosis for LC patients than conventional alpha-fetoprotein (AFP) detection. HCC patients were discriminated from LC with 78 % sensitivity and 64 % specificity. In comparison, AFP showed sensitivity and specificity of 38 % and 93 %, respectively. HCC was differentiated from CHB with 100 % sensitivity and specificity using the UPLC-MS approach. Identified lipids comprised glycerophosphocolines, glycerophosphoserines and glycerophosphoinositols.
UPLC-MS lipid profiling proved to be an efficient and convenient tool for diagnosis and screening of HCC in a high-risk population. |
| doi_str_mv | 10.1186/s12885-015-1995-1 |
| format | Article |
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An ultraperformance liquid chromatography mass spectrometry (UPLC-MS) lipidomic method was used to characterize serum profiles from HCC (n = 32), liver cirrhosis (LC) (n = 30), CHB (n = 25), and healthy subjects (n = 34). Patients were diagnosed by clinical laboratory and imaging evidence and all presented with CHB while healthy controls had normal liver function and no infectious diseases.
The UPLC-MS-based serum lipidomic profile provided more accurate diagnosis for LC patients than conventional alpha-fetoprotein (AFP) detection. HCC patients were discriminated from LC with 78 % sensitivity and 64 % specificity. In comparison, AFP showed sensitivity and specificity of 38 % and 93 %, respectively. HCC was differentiated from CHB with 100 % sensitivity and specificity using the UPLC-MS approach. Identified lipids comprised glycerophosphocolines, glycerophosphoserines and glycerophosphoinositols.
UPLC-MS lipid profiling proved to be an efficient and convenient tool for diagnosis and screening of HCC in a high-risk population.</description><identifier>ISSN: 1471-2407</identifier><identifier>EISSN: 1471-2407</identifier><identifier>DOI: 10.1186/s12885-015-1995-1</identifier><identifier>PMID: 26680993</identifier><language>eng</language><publisher>England: BioMed Central Ltd</publisher><subject>Adult ; Aged ; Biological markers ; Biomarkers ; Biomarkers, Tumor - blood ; Carcinoma, Hepatocellular - blood ; Carcinoma, Hepatocellular - diagnosis ; Carcinoma, Hepatocellular - virology ; Complications and side effects ; Diagnosis, Differential ; Early Detection of Cancer ; Female ; Gas Chromatography-Mass Spectrometry - methods ; Hepatitis B ; Hepatitis B, Chronic - blood ; Hepatitis B, Chronic - diagnosis ; Hepatoma ; Humans ; Lipids ; Lipids - blood ; Liquid chromatography ; Liver cancer ; Liver Cirrhosis - blood ; Liver Cirrhosis - diagnosis ; Liver Neoplasms - blood ; Liver Neoplasms - diagnosis ; Liver Neoplasms - virology ; Male ; Middle Aged ; Risk factors ; Young Adult</subject><ispartof>BMC cancer, 2015-12, Vol.15 (983), p.985-985, Article 985</ispartof><rights>COPYRIGHT 2015 BioMed Central Ltd.</rights><rights>Copyright BioMed Central 2015</rights><rights>Passos-Castilho et al. 2015</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c559t-bd5ac4269a24abf4c2c1b264fd82c3b7e89ef82c2b3b2ebe9333e29b231725663</citedby><cites>FETCH-LOGICAL-c559t-bd5ac4269a24abf4c2c1b264fd82c3b7e89ef82c2b3b2ebe9333e29b231725663</cites><orcidid>0000-0002-4814-5191</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4683945/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4683945/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,315,728,781,785,865,886,27929,27930,53796,53798</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26680993$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Passos-Castilho, Ana Maria</creatorcontrib><creatorcontrib>Carvalho, Valdemir Melechco</creatorcontrib><creatorcontrib>Cardozo, Karina Helena Morais</creatorcontrib><creatorcontrib>Kikuchi, Luciana</creatorcontrib><creatorcontrib>Chagas, Aline Lopes</creatorcontrib><creatorcontrib>Gomes-Gouvêa, Michele Soares</creatorcontrib><creatorcontrib>Malta, Fernanda</creatorcontrib><creatorcontrib>de Seixas-Santos Nastri, Ana Catharina</creatorcontrib><creatorcontrib>Pinho, João Renato Rebello</creatorcontrib><creatorcontrib>Carrilho, Flair José</creatorcontrib><creatorcontrib>Granato, Celso Francisco Hernandes</creatorcontrib><title>Serum lipidomic profiling as a useful tool for screening potential biomarkers of hepatitis B-related hepatocellular carcinoma by ultraperformance liquid chromatography-mass spectrometry</title><title>BMC cancer</title><addtitle>BMC Cancer</addtitle><description>Chronic hepatitis B (CHB) virus infection is a major cause of hepatocellular carcinoma (HCC), as late diagnosis is the main factor for the poor survival of patients. There is an urgent need for accurate biomarkers for early diagnosis of HCC. The aim of the study was to explore the serum lipidome profiles of hepatitis B-related HCC to identify potential diagnostic biomarkers.
An ultraperformance liquid chromatography mass spectrometry (UPLC-MS) lipidomic method was used to characterize serum profiles from HCC (n = 32), liver cirrhosis (LC) (n = 30), CHB (n = 25), and healthy subjects (n = 34). Patients were diagnosed by clinical laboratory and imaging evidence and all presented with CHB while healthy controls had normal liver function and no infectious diseases.
The UPLC-MS-based serum lipidomic profile provided more accurate diagnosis for LC patients than conventional alpha-fetoprotein (AFP) detection. HCC patients were discriminated from LC with 78 % sensitivity and 64 % specificity. In comparison, AFP showed sensitivity and specificity of 38 % and 93 %, respectively. HCC was differentiated from CHB with 100 % sensitivity and specificity using the UPLC-MS approach. Identified lipids comprised glycerophosphocolines, glycerophosphoserines and glycerophosphoinositols.
UPLC-MS lipid profiling proved to be an efficient and convenient tool for diagnosis and screening of HCC in a high-risk population.</description><subject>Adult</subject><subject>Aged</subject><subject>Biological markers</subject><subject>Biomarkers</subject><subject>Biomarkers, Tumor - blood</subject><subject>Carcinoma, Hepatocellular - blood</subject><subject>Carcinoma, Hepatocellular - diagnosis</subject><subject>Carcinoma, Hepatocellular - virology</subject><subject>Complications and side effects</subject><subject>Diagnosis, Differential</subject><subject>Early Detection of Cancer</subject><subject>Female</subject><subject>Gas Chromatography-Mass Spectrometry - methods</subject><subject>Hepatitis B</subject><subject>Hepatitis B, Chronic - blood</subject><subject>Hepatitis B, Chronic - diagnosis</subject><subject>Hepatoma</subject><subject>Humans</subject><subject>Lipids</subject><subject>Lipids - blood</subject><subject>Liquid chromatography</subject><subject>Liver cancer</subject><subject>Liver Cirrhosis - blood</subject><subject>Liver Cirrhosis - diagnosis</subject><subject>Liver Neoplasms - blood</subject><subject>Liver Neoplasms - diagnosis</subject><subject>Liver Neoplasms - virology</subject><subject>Male</subject><subject>Middle Aged</subject><subject>Risk factors</subject><subject>Young Adult</subject><issn>1471-2407</issn><issn>1471-2407</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><recordid>eNptkttqFTEUhgdRbK0-gDcSEEQvpuYwp9wUavFQKAhWr0OSWbMnNTOZ5iDuR_PtzLBr3VtkYCZkff-fWSt_UTwn-JSQrnkbCO26usSkLgnn-fWgOCZVS0pa4fbh3vqoeBLCDcak7XD3uDiiTdNhztlx8esafJqQNYvp3WQ0WrwbjDXzBsmAJEoBhmRRdM6iwXkUtAeY1_LiIszRSIuUcZP038EH5AY0wiKjiSagd6UHKyP0uz2nwdpkpUdaem3mLEJqi5KNXi7gs_skZw35X26T6ZEefSai2-TquC0nGQIKC-iYtyH67dPi0SBtgGd335Pi24f3Xy8-lVefP15enF-Vuq55LFVfS13RhktaSTVUmmqiaFMNfUc1Uy10HIa8pIopCgo4YwwoV5SRltZNw06Ks53vktQEvc5Ne2nF4k1ueiucNOKwMptRbNwPUTUd41WdDV7fGXh3myBEMZmwzkLO4FIQpK1xRXlLaEZf_oPeuOTn3F6mWl5Thknzl9pIC8LMg8vn6tVUnFc1aVpMWJup0_9Q-ekh37ObIV8zHAreHAgyE-Fn3MgUgri8_nLIvtpjR5A2jsHZFI2bwyFIdqD2LgQPw_3gCBZriMUuxCKHWKwhFiRrXuxP_F7xJ7XsN4lJ8Qc</recordid><startdate>20151218</startdate><enddate>20151218</enddate><creator>Passos-Castilho, Ana Maria</creator><creator>Carvalho, Valdemir Melechco</creator><creator>Cardozo, Karina Helena Morais</creator><creator>Kikuchi, Luciana</creator><creator>Chagas, Aline Lopes</creator><creator>Gomes-Gouvêa, Michele Soares</creator><creator>Malta, Fernanda</creator><creator>de Seixas-Santos Nastri, Ana Catharina</creator><creator>Pinho, João Renato Rebello</creator><creator>Carrilho, Flair José</creator><creator>Granato, Celso Francisco Hernandes</creator><general>BioMed Central Ltd</general><general>BioMed Central</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>ISR</scope><scope>3V.</scope><scope>7TO</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>H94</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0002-4814-5191</orcidid></search><sort><creationdate>20151218</creationdate><title>Serum lipidomic profiling as a useful tool for screening potential biomarkers of hepatitis B-related hepatocellular carcinoma by ultraperformance liquid chromatography-mass spectrometry</title><author>Passos-Castilho, Ana Maria ; Carvalho, Valdemir Melechco ; Cardozo, Karina Helena Morais ; Kikuchi, Luciana ; Chagas, Aline Lopes ; Gomes-Gouvêa, Michele Soares ; Malta, Fernanda ; de Seixas-Santos Nastri, Ana Catharina ; Pinho, João Renato Rebello ; Carrilho, Flair José ; Granato, Celso Francisco Hernandes</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c559t-bd5ac4269a24abf4c2c1b264fd82c3b7e89ef82c2b3b2ebe9333e29b231725663</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Adult</topic><topic>Aged</topic><topic>Biological markers</topic><topic>Biomarkers</topic><topic>Biomarkers, Tumor - blood</topic><topic>Carcinoma, Hepatocellular - blood</topic><topic>Carcinoma, Hepatocellular - diagnosis</topic><topic>Carcinoma, Hepatocellular - virology</topic><topic>Complications and side effects</topic><topic>Diagnosis, Differential</topic><topic>Early Detection of Cancer</topic><topic>Female</topic><topic>Gas Chromatography-Mass Spectrometry - methods</topic><topic>Hepatitis B</topic><topic>Hepatitis B, Chronic - blood</topic><topic>Hepatitis B, Chronic - diagnosis</topic><topic>Hepatoma</topic><topic>Humans</topic><topic>Lipids</topic><topic>Lipids - blood</topic><topic>Liquid chromatography</topic><topic>Liver cancer</topic><topic>Liver Cirrhosis - blood</topic><topic>Liver Cirrhosis - diagnosis</topic><topic>Liver Neoplasms - blood</topic><topic>Liver Neoplasms - diagnosis</topic><topic>Liver Neoplasms - virology</topic><topic>Male</topic><topic>Middle Aged</topic><topic>Risk factors</topic><topic>Young Adult</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Passos-Castilho, Ana Maria</creatorcontrib><creatorcontrib>Carvalho, Valdemir Melechco</creatorcontrib><creatorcontrib>Cardozo, Karina Helena Morais</creatorcontrib><creatorcontrib>Kikuchi, Luciana</creatorcontrib><creatorcontrib>Chagas, Aline Lopes</creatorcontrib><creatorcontrib>Gomes-Gouvêa, Michele Soares</creatorcontrib><creatorcontrib>Malta, Fernanda</creatorcontrib><creatorcontrib>de Seixas-Santos Nastri, Ana Catharina</creatorcontrib><creatorcontrib>Pinho, João Renato Rebello</creatorcontrib><creatorcontrib>Carrilho, Flair José</creatorcontrib><creatorcontrib>Granato, Celso Francisco Hernandes</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale In Context: Science</collection><collection>ProQuest Central (Corporate)</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Access via ProQuest (Open Access)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>BMC cancer</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Passos-Castilho, Ana Maria</au><au>Carvalho, Valdemir Melechco</au><au>Cardozo, Karina Helena Morais</au><au>Kikuchi, Luciana</au><au>Chagas, Aline Lopes</au><au>Gomes-Gouvêa, Michele Soares</au><au>Malta, Fernanda</au><au>de Seixas-Santos Nastri, Ana Catharina</au><au>Pinho, João Renato Rebello</au><au>Carrilho, Flair José</au><au>Granato, Celso Francisco Hernandes</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Serum lipidomic profiling as a useful tool for screening potential biomarkers of hepatitis B-related hepatocellular carcinoma by ultraperformance liquid chromatography-mass spectrometry</atitle><jtitle>BMC cancer</jtitle><addtitle>BMC Cancer</addtitle><date>2015-12-18</date><risdate>2015</risdate><volume>15</volume><issue>983</issue><spage>985</spage><epage>985</epage><pages>985-985</pages><artnum>985</artnum><issn>1471-2407</issn><eissn>1471-2407</eissn><abstract>Chronic hepatitis B (CHB) virus infection is a major cause of hepatocellular carcinoma (HCC), as late diagnosis is the main factor for the poor survival of patients. There is an urgent need for accurate biomarkers for early diagnosis of HCC. The aim of the study was to explore the serum lipidome profiles of hepatitis B-related HCC to identify potential diagnostic biomarkers.
An ultraperformance liquid chromatography mass spectrometry (UPLC-MS) lipidomic method was used to characterize serum profiles from HCC (n = 32), liver cirrhosis (LC) (n = 30), CHB (n = 25), and healthy subjects (n = 34). Patients were diagnosed by clinical laboratory and imaging evidence and all presented with CHB while healthy controls had normal liver function and no infectious diseases.
The UPLC-MS-based serum lipidomic profile provided more accurate diagnosis for LC patients than conventional alpha-fetoprotein (AFP) detection. HCC patients were discriminated from LC with 78 % sensitivity and 64 % specificity. In comparison, AFP showed sensitivity and specificity of 38 % and 93 %, respectively. HCC was differentiated from CHB with 100 % sensitivity and specificity using the UPLC-MS approach. Identified lipids comprised glycerophosphocolines, glycerophosphoserines and glycerophosphoinositols.
UPLC-MS lipid profiling proved to be an efficient and convenient tool for diagnosis and screening of HCC in a high-risk population.</abstract><cop>England</cop><pub>BioMed Central Ltd</pub><pmid>26680993</pmid><doi>10.1186/s12885-015-1995-1</doi><tpages>1</tpages><orcidid>https://orcid.org/0000-0002-4814-5191</orcidid><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central Open Access; Springer Nature OA Free Journals; PubMed Central; SpringerLink Journals - AutoHoldings |
| subjects | Adult Aged Biological markers Biomarkers Biomarkers, Tumor - blood Carcinoma, Hepatocellular - blood Carcinoma, Hepatocellular - diagnosis Carcinoma, Hepatocellular - virology Complications and side effects Diagnosis, Differential Early Detection of Cancer Female Gas Chromatography-Mass Spectrometry - methods Hepatitis B Hepatitis B, Chronic - blood Hepatitis B, Chronic - diagnosis Hepatoma Humans Lipids Lipids - blood Liquid chromatography Liver cancer Liver Cirrhosis - blood Liver Cirrhosis - diagnosis Liver Neoplasms - blood Liver Neoplasms - diagnosis Liver Neoplasms - virology Male Middle Aged Risk factors Young Adult |
| title | Serum lipidomic profiling as a useful tool for screening potential biomarkers of hepatitis B-related hepatocellular carcinoma by ultraperformance liquid chromatography-mass spectrometry |
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