Site-specific replacement of the thymine methyl group by fluorine in thrombin binding aptamer significantly improves structural stability and anticoagulant activity

Here we report investigations, based on circular dichroism, nuclear magnetic resonance spectroscopy, molecular modelling, differential scanning calorimetry and prothrombin time assay, on analogues of the thrombin binding aptamer (TBA) in which individual thymidines were replaced by 5-fluoro-2'-...

Ausführliche Beschreibung

Bibliographische Detailangaben

Veröffentlicht in:	Nucleic acids research 2015-12, Vol.43 (22), p.10602-10611
Hauptverfasser:	Virgilio, Antonella, Petraccone, Luigi, Vellecco, Valentina, Bucci, Mariarosaria, Varra, Michela, Irace, Carlo, Santamaria, Rita, Pepe, Antonietta, Mayol, Luciano, Esposito, Veronica, Galeone, Aldo
Format:	Artikel
Sprache:	eng
Schlagworte:	Anticoagulants - chemistry Aptamers, Nucleotide - chemistry Chemical Biology and Nucleic Acid Chemistry Circular Dichroism Deoxyribonucleases Fluorine - chemistry Magnetic Resonance Spectroscopy Models, Molecular Nucleic Acid Denaturation Prothrombin Time Thermodynamics Thymidine - chemistry
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!