Submerged fermentation production and characterization of intracellular triterpenoids from Ganoderma lucidum using HPLC-ESI-MS
As the main bioactive metabolites of Ganoderma lucidum , triterpenoids have various pharmacological effects. In this paper, the nutritional requirements and culture conditions of a submerged culture of G. lucidum were optimized using the response surface methodology; maximum mycelia biomass and intr...
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| Veröffentlicht in: | Journal of Zhejiang University. B. Science 2015-12, Vol.16 (12), p.998-1010 |
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| creator | Cui, Mei-lin Yang, Huan-yi He, Guo-qing |
| description | As the main bioactive metabolites of
Ganoderma lucidum
, triterpenoids have various pharmacological effects. In this paper, the nutritional requirements and culture conditions of a submerged culture of
G. lucidum
were optimized using the response surface methodology; maximum mycelia biomass and intracellular triterpenoid production reached 1.87 g/100 ml and 93.21 mg/100 ml, respectively, for a culture consisting of wort 4.10% (0.041 g/ml) and yeast extract 1.89% (0.0189 g/ml), pH 5.40. For the first time, we established that wort, which is cheap and abundant, can replace the more commonly used glucose as the sole source of carbohydrate. Using high-performance liquid chromatography-electrospray ionization-mass spectrometry (HPLC-ESI-MS), 10 major ganoderic acids were tentatively identified based on the predominant fragmentation pathways with the elimination of H
2
O and CO
2
, as well as cleavage of the D-ring. |
| doi_str_mv | 10.1631/jzus.B1500147 |
| format | Article |
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Ganoderma lucidum
, triterpenoids have various pharmacological effects. In this paper, the nutritional requirements and culture conditions of a submerged culture of
G. lucidum
were optimized using the response surface methodology; maximum mycelia biomass and intracellular triterpenoid production reached 1.87 g/100 ml and 93.21 mg/100 ml, respectively, for a culture consisting of wort 4.10% (0.041 g/ml) and yeast extract 1.89% (0.0189 g/ml), pH 5.40. For the first time, we established that wort, which is cheap and abundant, can replace the more commonly used glucose as the sole source of carbohydrate. Using high-performance liquid chromatography-electrospray ionization-mass spectrometry (HPLC-ESI-MS), 10 major ganoderic acids were tentatively identified based on the predominant fragmentation pathways with the elimination of H
2
O and CO
2
, as well as cleavage of the D-ring.</description><identifier>ISSN: 1673-1581</identifier><identifier>EISSN: 1862-1783</identifier><identifier>DOI: 10.1631/jzus.B1500147</identifier><identifier>PMID: 26642183</identifier><language>eng</language><publisher>Hangzhou: Zhejiang University Press</publisher><subject>Acids ; Bioactive compounds ; Biocompatibility ; Biomass ; Biomedical and Life Sciences ; Biomedical materials ; Biomedicine ; Carbohydrates ; Carbon dioxide ; Chromatography, High Pressure Liquid - methods ; Cleavage ; Fermentation ; Fragmentation ; Ganoderma lucidum ; Glucose ; High performance liquid chromatography ; Hydrogen-Ion Concentration ; Intracellular ; Ionization ; Ions ; Liquid chromatography ; Mass spectrometry ; Mass spectroscopy ; Metabolites ; Molecular Structure ; Mushrooms ; Mycelia ; Nitrogen - metabolism ; Nutritional requirements ; Pharmacology ; Reishi - growth & development ; Reishi - metabolism ; Response surface methodology ; Spectrometry, Mass, Electrospray Ionization - methods ; Surgical implants ; Triterpenes - chemistry ; Triterpenes - metabolism ; Triterpenoids ; Water analysis ; Wort ; Yeast</subject><ispartof>Journal of Zhejiang University. B. Science, 2015-12, Vol.16 (12), p.998-1010</ispartof><rights>Zhejiang University and Springer-Verlag Berlin Heidelberg 2015</rights><rights>Journal of Zhejiang University SCIENCE B is a copyright of Springer, 2015.</rights><rights>Copyright © Zhejiang University and Springer-Verlag Berlin Heidelberg 2015 2015</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c487t-75665a822f6925d275e38fa53898c928dfee0040f9212438839fc36c5b75bb823</citedby><cites>FETCH-LOGICAL-c487t-75665a822f6925d275e38fa53898c928dfee0040f9212438839fc36c5b75bb823</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4678043/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4678043/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,27923,27924,41487,42556,51318,53790,53792</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26642183$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Cui, Mei-lin</creatorcontrib><creatorcontrib>Yang, Huan-yi</creatorcontrib><creatorcontrib>He, Guo-qing</creatorcontrib><title>Submerged fermentation production and characterization of intracellular triterpenoids from Ganoderma lucidum using HPLC-ESI-MS</title><title>Journal of Zhejiang University. B. Science</title><addtitle>J. Zhejiang Univ. Sci. B</addtitle><addtitle>J Zhejiang Univ Sci B</addtitle><description>As the main bioactive metabolites of
Ganoderma lucidum
, triterpenoids have various pharmacological effects. In this paper, the nutritional requirements and culture conditions of a submerged culture of
G. lucidum
were optimized using the response surface methodology; maximum mycelia biomass and intracellular triterpenoid production reached 1.87 g/100 ml and 93.21 mg/100 ml, respectively, for a culture consisting of wort 4.10% (0.041 g/ml) and yeast extract 1.89% (0.0189 g/ml), pH 5.40. For the first time, we established that wort, which is cheap and abundant, can replace the more commonly used glucose as the sole source of carbohydrate. Using high-performance liquid chromatography-electrospray ionization-mass spectrometry (HPLC-ESI-MS), 10 major ganoderic acids were tentatively identified based on the predominant fragmentation pathways with the elimination of H
2
O and CO
2
, as well as cleavage of the D-ring.</description><subject>Acids</subject><subject>Bioactive compounds</subject><subject>Biocompatibility</subject><subject>Biomass</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedical materials</subject><subject>Biomedicine</subject><subject>Carbohydrates</subject><subject>Carbon dioxide</subject><subject>Chromatography, High Pressure Liquid - methods</subject><subject>Cleavage</subject><subject>Fermentation</subject><subject>Fragmentation</subject><subject>Ganoderma lucidum</subject><subject>Glucose</subject><subject>High performance liquid chromatography</subject><subject>Hydrogen-Ion Concentration</subject><subject>Intracellular</subject><subject>Ionization</subject><subject>Ions</subject><subject>Liquid chromatography</subject><subject>Mass spectrometry</subject><subject>Mass spectroscopy</subject><subject>Metabolites</subject><subject>Molecular Structure</subject><subject>Mushrooms</subject><subject>Mycelia</subject><subject>Nitrogen - metabolism</subject><subject>Nutritional requirements</subject><subject>Pharmacology</subject><subject>Reishi - growth & development</subject><subject>Reishi - metabolism</subject><subject>Response surface methodology</subject><subject>Spectrometry, Mass, Electrospray Ionization - methods</subject><subject>Surgical implants</subject><subject>Triterpenes - chemistry</subject><subject>Triterpenes - metabolism</subject><subject>Triterpenoids</subject><subject>Water analysis</subject><subject>Wort</subject><subject>Yeast</subject><issn>1673-1581</issn><issn>1862-1783</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqNks1rFDEYxgdRbK0evUrAi5dZ853MRbBLv2BFYfUcsplkm2UmWZOJYA_-7c24baniwVNe8vx48j5v3qZ5jeACcYLe725KXpwiBiGi4klzjCTHLRKSPK01F6RFTKKj5kXOOwgphYI_b44w5xQjSY6bX-uyGW3a2h44m0YbJj35GMA-xb6Y36UOPTDXOmkz2eRvDnp0wIep3tlhKINOYEq-ynsbou8zcCmO4EKH2FdTDYZifF9GULIPW3D5ZbVsz9ZX7af1y-aZ00O2r-7Ok-bb-dnX5WW7-nxxtfy4ag2VYmoF45xpibHjHWY9FswS6TQjspOmw7J31tZ00HUYYUqkJJ0zhBu2EWyzkZicNB8Ovvs5bm_s3Pug9smPOv1UUXv1pxL8tdrGH4pyISEl1eDdnUGK34vNkxp9nsPrYGPJCgkmMe06hv8DpYLgjkha0bd_obtYUqiTUKhDhJPqCCvVHiiTYs7Juoe-EVTzEqh5CdT9ElT-zeOwD_T9r1dgcQBylcLWpkfP_tPxFmiWvsE</recordid><startdate>20151201</startdate><enddate>20151201</enddate><creator>Cui, Mei-lin</creator><creator>Yang, Huan-yi</creator><creator>He, Guo-qing</creator><general>Zhejiang University Press</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QO</scope><scope>7QP</scope><scope>7TK</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>BKSAR</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>M7S</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PCBAR</scope><scope>PDBOC</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>7X8</scope><scope>7T7</scope><scope>C1K</scope><scope>5PM</scope></search><sort><creationdate>20151201</creationdate><title>Submerged fermentation production and characterization of intracellular triterpenoids from Ganoderma lucidum using HPLC-ESI-MS</title><author>Cui, Mei-lin ; Yang, Huan-yi ; He, Guo-qing</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c487t-75665a822f6925d275e38fa53898c928dfee0040f9212438839fc36c5b75bb823</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Acids</topic><topic>Bioactive compounds</topic><topic>Biocompatibility</topic><topic>Biomass</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedical materials</topic><topic>Biomedicine</topic><topic>Carbohydrates</topic><topic>Carbon dioxide</topic><topic>Chromatography, High Pressure Liquid - methods</topic><topic>Cleavage</topic><topic>Fermentation</topic><topic>Fragmentation</topic><topic>Ganoderma lucidum</topic><topic>Glucose</topic><topic>High performance liquid chromatography</topic><topic>Hydrogen-Ion Concentration</topic><topic>Intracellular</topic><topic>Ionization</topic><topic>Ions</topic><topic>Liquid chromatography</topic><topic>Mass spectrometry</topic><topic>Mass spectroscopy</topic><topic>Metabolites</topic><topic>Molecular Structure</topic><topic>Mushrooms</topic><topic>Mycelia</topic><topic>Nitrogen - metabolism</topic><topic>Nutritional requirements</topic><topic>Pharmacology</topic><topic>Reishi - growth & development</topic><topic>Reishi - metabolism</topic><topic>Response surface methodology</topic><topic>Spectrometry, Mass, Electrospray Ionization - methods</topic><topic>Surgical implants</topic><topic>Triterpenes - chemistry</topic><topic>Triterpenes - metabolism</topic><topic>Triterpenoids</topic><topic>Water analysis</topic><topic>Wort</topic><topic>Yeast</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Cui, Mei-lin</creatorcontrib><creatorcontrib>Yang, Huan-yi</creatorcontrib><creatorcontrib>He, Guo-qing</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Biotechnology Research Abstracts</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies & Aerospace Collection</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>Earth, Atmospheric & Aquatic Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>ProQuest Engineering Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biological Science Database</collection><collection>Engineering Database</collection><collection>Advanced Technologies & Aerospace Database</collection><collection>ProQuest Advanced Technologies & Aerospace Collection</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>Earth, Atmospheric & Aquatic Science Database</collection><collection>Materials Science Collection</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Engineering Collection</collection><collection>Environmental Science Collection</collection><collection>MEDLINE - Academic</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Environmental Sciences and Pollution Management</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of Zhejiang University. B. Science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Cui, Mei-lin</au><au>Yang, Huan-yi</au><au>He, Guo-qing</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Submerged fermentation production and characterization of intracellular triterpenoids from Ganoderma lucidum using HPLC-ESI-MS</atitle><jtitle>Journal of Zhejiang University. B. Science</jtitle><stitle>J. Zhejiang Univ. Sci. B</stitle><addtitle>J Zhejiang Univ Sci B</addtitle><date>2015-12-01</date><risdate>2015</risdate><volume>16</volume><issue>12</issue><spage>998</spage><epage>1010</epage><pages>998-1010</pages><issn>1673-1581</issn><eissn>1862-1783</eissn><abstract>As the main bioactive metabolites of
Ganoderma lucidum
, triterpenoids have various pharmacological effects. In this paper, the nutritional requirements and culture conditions of a submerged culture of
G. lucidum
were optimized using the response surface methodology; maximum mycelia biomass and intracellular triterpenoid production reached 1.87 g/100 ml and 93.21 mg/100 ml, respectively, for a culture consisting of wort 4.10% (0.041 g/ml) and yeast extract 1.89% (0.0189 g/ml), pH 5.40. For the first time, we established that wort, which is cheap and abundant, can replace the more commonly used glucose as the sole source of carbohydrate. Using high-performance liquid chromatography-electrospray ionization-mass spectrometry (HPLC-ESI-MS), 10 major ganoderic acids were tentatively identified based on the predominant fragmentation pathways with the elimination of H
2
O and CO
2
, as well as cleavage of the D-ring.</abstract><cop>Hangzhou</cop><pub>Zhejiang University Press</pub><pmid>26642183</pmid><doi>10.1631/jzus.B1500147</doi><tpages>13</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Journal of Zhejiang University. B. Science, 2015-12, Vol.16 (12), p.998-1010 |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; Free Full-Text Journals in Chemistry; SpringerLink Journals - AutoHoldings |
| subjects | Acids Bioactive compounds Biocompatibility Biomass Biomedical and Life Sciences Biomedical materials Biomedicine Carbohydrates Carbon dioxide Chromatography, High Pressure Liquid - methods Cleavage Fermentation Fragmentation Ganoderma lucidum Glucose High performance liquid chromatography Hydrogen-Ion Concentration Intracellular Ionization Ions Liquid chromatography Mass spectrometry Mass spectroscopy Metabolites Molecular Structure Mushrooms Mycelia Nitrogen - metabolism Nutritional requirements Pharmacology Reishi - growth & development Reishi - metabolism Response surface methodology Spectrometry, Mass, Electrospray Ionization - methods Surgical implants Triterpenes - chemistry Triterpenes - metabolism Triterpenoids Water analysis Wort Yeast |
| title | Submerged fermentation production and characterization of intracellular triterpenoids from Ganoderma lucidum using HPLC-ESI-MS |
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