Clonal population expansion in an outbreak of Plasmodium falciparum on the northwest coast of Ecuador
Determining the source of malaria outbreaks in Ecuador and identifying remaining transmission foci will help in malaria elimination efforts. In this study, the genetic signatures of Plasmodium falciparum isolates, obtained from an outbreak that occurred in northwest Ecuador from 2012 to 2013, were c...
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| Veröffentlicht in: | Malaria journal 2015-12, Vol.13 Suppl 1 (1), p.497-497, Article 497 |
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| creator | Sáenz, Fabián E Morton, Lindsay C Okoth, Sheila Akinyi Valenzuela, Gabriela Vera-Arias, Claudia A Vélez-Álvarez, Eileen Lucchi, Naomi W Castro, L Enrique Udhayakumar, Venkatachalam |
| description | Determining the source of malaria outbreaks in Ecuador and identifying remaining transmission foci will help in malaria elimination efforts. In this study, the genetic signatures of Plasmodium falciparum isolates, obtained from an outbreak that occurred in northwest Ecuador from 2012 to 2013, were characterized.
Molecular investigation of the outbreak was performed using neutral microsatellites, drug resistance markers and pfhrp2 and pfhrp3 genotyping.
A majority of parasite isolates (31/32) from this outbreak were of a single clonal type that matched a clonal lineage previously described on the northern coast of Peru and a historical isolate from Ecuador. All but one isolate carried a chloroquine-resistant pfcrt genotype and sulfadoxine- and pyrimethamine-sensitive pfdhps and pfdhfr genotypes. Pfmdr1 mutations were identified in codons 184 and 1042. In addition, most samples (97 %) showed presence of pfhrp2 gene.
This study indicates that parasites from a single clonal lineage largely contributed to this outbreak and this lineage was found to be genetically related to a lineage previously reported in the Peruvian coast and historical Ecuadorian parasites. |
| doi_str_mv | 10.1186/s12936-015-1019-2 |
| format | Article |
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Molecular investigation of the outbreak was performed using neutral microsatellites, drug resistance markers and pfhrp2 and pfhrp3 genotyping.
A majority of parasite isolates (31/32) from this outbreak were of a single clonal type that matched a clonal lineage previously described on the northern coast of Peru and a historical isolate from Ecuador. All but one isolate carried a chloroquine-resistant pfcrt genotype and sulfadoxine- and pyrimethamine-sensitive pfdhps and pfdhfr genotypes. Pfmdr1 mutations were identified in codons 184 and 1042. In addition, most samples (97 %) showed presence of pfhrp2 gene.
This study indicates that parasites from a single clonal lineage largely contributed to this outbreak and this lineage was found to be genetically related to a lineage previously reported in the Peruvian coast and historical Ecuadorian parasites.</description><identifier>ISSN: 1475-2875</identifier><identifier>EISSN: 1475-2875</identifier><identifier>DOI: 10.1186/s12936-015-1019-2</identifier><identifier>PMID: 26651993</identifier><language>eng</language><publisher>England: BioMed Central Ltd</publisher><subject>Codon ; Drug resistance ; Genetic aspects ; Malaria ; Plasmodium falciparum</subject><ispartof>Malaria journal, 2015-12, Vol.13 Suppl 1 (1), p.497-497, Article 497</ispartof><rights>COPYRIGHT 2015 BioMed Central Ltd.</rights><rights>Copyright BioMed Central 2015</rights><rights>Sáenz et al. 2015</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c494t-9648adf88354b439589adee0c7a0bae4ef50b0242384f02a5459db43b5ea0d7d3</citedby><cites>FETCH-LOGICAL-c494t-9648adf88354b439589adee0c7a0bae4ef50b0242384f02a5459db43b5ea0d7d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4676133/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4676133/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26651993$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sáenz, Fabián E</creatorcontrib><creatorcontrib>Morton, Lindsay C</creatorcontrib><creatorcontrib>Okoth, Sheila Akinyi</creatorcontrib><creatorcontrib>Valenzuela, Gabriela</creatorcontrib><creatorcontrib>Vera-Arias, Claudia A</creatorcontrib><creatorcontrib>Vélez-Álvarez, Eileen</creatorcontrib><creatorcontrib>Lucchi, Naomi W</creatorcontrib><creatorcontrib>Castro, L Enrique</creatorcontrib><creatorcontrib>Udhayakumar, Venkatachalam</creatorcontrib><title>Clonal population expansion in an outbreak of Plasmodium falciparum on the northwest coast of Ecuador</title><title>Malaria journal</title><addtitle>Malar J</addtitle><description>Determining the source of malaria outbreaks in Ecuador and identifying remaining transmission foci will help in malaria elimination efforts. In this study, the genetic signatures of Plasmodium falciparum isolates, obtained from an outbreak that occurred in northwest Ecuador from 2012 to 2013, were characterized.
Molecular investigation of the outbreak was performed using neutral microsatellites, drug resistance markers and pfhrp2 and pfhrp3 genotyping.
A majority of parasite isolates (31/32) from this outbreak were of a single clonal type that matched a clonal lineage previously described on the northern coast of Peru and a historical isolate from Ecuador. All but one isolate carried a chloroquine-resistant pfcrt genotype and sulfadoxine- and pyrimethamine-sensitive pfdhps and pfdhfr genotypes. Pfmdr1 mutations were identified in codons 184 and 1042. In addition, most samples (97 %) showed presence of pfhrp2 gene.
This study indicates that parasites from a single clonal lineage largely contributed to this outbreak and this lineage was found to be genetically related to a lineage previously reported in the Peruvian coast and historical Ecuadorian parasites.</description><subject>Codon</subject><subject>Drug resistance</subject><subject>Genetic aspects</subject><subject>Malaria</subject><subject>Plasmodium falciparum</subject><issn>1475-2875</issn><issn>1475-2875</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><recordid>eNptkstu1TAQhiMEoqXwAGxQJDZsUnx3vEGqjtqCVAkWsLYmzqTHJbGDnXB5exydUlqELI1H9vfPaEZ_Vb2k5JTSVr3NlBmuGkJlQwk1DXtUHVOhZcNaLR_fy4-qZznfEEJ1q9nT6ogpJakx_LjC3RgDjPUc53WExcdQ488ZQt4yH2oIdVyXLiF8reNQfxohT7H361QPMDo_QyppQZc91iGmZf8D81K7CCUW_tyt0Mf0vHpS8Iwvbu-T6svF-efd--bq4-WH3dlV44QRS2OUaKEf2pZL0QluZGugRyROA-kABQ6SdIQJxlsxEAZSSNMXsJMIpNc9P6neHerOazdh7zAsCUY7Jz9B-mUjePvwJ_i9vY7frVBaUc5LgTe3BVL8tpZR7OSzw3GEgHHNlmphjFGUyoK-_ge9iWsqu9woXUbhJfylrmFE68MQS1-3FbVnQrVGcqa2tqf_ocrpcfIuBhx8eX8goAeBSzHnhMPdjJTYzRv24A1bvGE3b1hWNK_uL-dO8ccM_Dd0crUb</recordid><startdate>20151210</startdate><enddate>20151210</enddate><creator>Sáenz, Fabián E</creator><creator>Morton, Lindsay C</creator><creator>Okoth, Sheila Akinyi</creator><creator>Valenzuela, Gabriela</creator><creator>Vera-Arias, Claudia A</creator><creator>Vélez-Álvarez, Eileen</creator><creator>Lucchi, Naomi W</creator><creator>Castro, L Enrique</creator><creator>Udhayakumar, Venkatachalam</creator><general>BioMed Central Ltd</general><general>BioMed Central</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7SS</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8C1</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>F1W</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>H94</scope><scope>H95</scope><scope>H97</scope><scope>K9.</scope><scope>L.G</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20151210</creationdate><title>Clonal population expansion in an outbreak of Plasmodium falciparum on the northwest coast of Ecuador</title><author>Sáenz, Fabián E ; 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In this study, the genetic signatures of Plasmodium falciparum isolates, obtained from an outbreak that occurred in northwest Ecuador from 2012 to 2013, were characterized.
Molecular investigation of the outbreak was performed using neutral microsatellites, drug resistance markers and pfhrp2 and pfhrp3 genotyping.
A majority of parasite isolates (31/32) from this outbreak were of a single clonal type that matched a clonal lineage previously described on the northern coast of Peru and a historical isolate from Ecuador. All but one isolate carried a chloroquine-resistant pfcrt genotype and sulfadoxine- and pyrimethamine-sensitive pfdhps and pfdhfr genotypes. Pfmdr1 mutations were identified in codons 184 and 1042. In addition, most samples (97 %) showed presence of pfhrp2 gene.
This study indicates that parasites from a single clonal lineage largely contributed to this outbreak and this lineage was found to be genetically related to a lineage previously reported in the Peruvian coast and historical Ecuadorian parasites.</abstract><cop>England</cop><pub>BioMed Central Ltd</pub><pmid>26651993</pmid><doi>10.1186/s12936-015-1019-2</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Malaria journal, 2015-12, Vol.13 Suppl 1 (1), p.497-497, Article 497 |
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| source | DOAJ Directory of Open Access Journals; PubMed Central Open Access; Springer Nature OA Free Journals; EZB-FREE-00999 freely available EZB journals; PubMed Central; SpringerLink Journals - AutoHoldings |
| subjects | Codon Drug resistance Genetic aspects Malaria Plasmodium falciparum |
| title | Clonal population expansion in an outbreak of Plasmodium falciparum on the northwest coast of Ecuador |
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