Effect of Cyclodextrins on Morphology and Barrier Characteristics of Isolated Rabbit Corneas
The objective of the present study is to investigate the confounding effects, if any, of beta-cyclodextrins (βCDs) on corneal permeability coefficients obtained from in vitro transmembrane diffusion studies. Transcorneal permeability studies were carried out with 2-hydroxypropyl-beta-cyclodextrin (H...
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| creator | Adelli, Goutham R. Balguri, Sai Prachetan Majumdar, Soumyajit |
| description | The objective of the present study is to investigate the confounding effects, if any, of beta-cyclodextrins (βCDs) on corneal permeability coefficients obtained from
in vitro
transmembrane diffusion studies. Transcorneal permeability studies were carried out with 2-hydroxypropyl-beta-cyclodextrin (HPβCD) and randomly methylated-beta-cyclodextrin (RMβCD) at 5 and 2.5%
w
/
v
in isotonic phosphate-buffered solution (IPBS) (pH 7.4). Rabbit corneas received from Pel-Freez Biologicals® were used for these studies. Propranolol hydrochloride (PHCl) (1 mg/mL) was used as the paracellular permeability marker. A series of permeation studies were carried out with IPBS as the control, with CDs on the donor side only, CDs on the receiver side only, and CDs on both the donor and receiver sides. At the end of 1 or 3 h, corneas were collected and fixed using a solution containing 2%
v
/
v
glutaraldehyde + 2%
w
/
v
paraformaldehyde + IPBS and histological examinations were performed (Excalibur Pathology, Inc). The order of transcorneal permeability of PHCl was found to be CDs on the receiver side > control (no CDs) ≈ CDs on both the receiver and donor sides > CDs on the donor side. Histology studies revealed that the corneal epithelial and endothelial layers remained intact in the control sets. Damage to the cornea was observed in the order of CDs on the receiver side > CDs on the donor side > CDs on both sides > control. The use of CDs in solutions for
in vitro
permeation experiments with rabbit corneas needs to be carefully considered to avoid confounding effects in the data obtained. |
| doi_str_mv | 10.1208/s12249-015-0315-z |
| format | Article |
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in vitro
transmembrane diffusion studies. Transcorneal permeability studies were carried out with 2-hydroxypropyl-beta-cyclodextrin (HPβCD) and randomly methylated-beta-cyclodextrin (RMβCD) at 5 and 2.5%
w
/
v
in isotonic phosphate-buffered solution (IPBS) (pH 7.4). Rabbit corneas received from Pel-Freez Biologicals® were used for these studies. Propranolol hydrochloride (PHCl) (1 mg/mL) was used as the paracellular permeability marker. A series of permeation studies were carried out with IPBS as the control, with CDs on the donor side only, CDs on the receiver side only, and CDs on both the donor and receiver sides. At the end of 1 or 3 h, corneas were collected and fixed using a solution containing 2%
v
/
v
glutaraldehyde + 2%
w
/
v
paraformaldehyde + IPBS and histological examinations were performed (Excalibur Pathology, Inc). The order of transcorneal permeability of PHCl was found to be CDs on the receiver side > control (no CDs) ≈ CDs on both the receiver and donor sides > CDs on the donor side. Histology studies revealed that the corneal epithelial and endothelial layers remained intact in the control sets. Damage to the cornea was observed in the order of CDs on the receiver side > CDs on the donor side > CDs on both sides > control. The use of CDs in solutions for
in vitro
permeation experiments with rabbit corneas needs to be carefully considered to avoid confounding effects in the data obtained.</description><identifier>ISSN: 1530-9932</identifier><identifier>EISSN: 1530-9932</identifier><identifier>DOI: 10.1208/s12249-015-0315-z</identifier><identifier>PMID: 25771740</identifier><language>eng</language><publisher>New York: Springer US</publisher><subject>2-Hydroxypropyl-beta-cyclodextrin ; Animals ; beta-Cyclodextrins - pharmacology ; beta-Cyclodextrins - toxicity ; Biochemistry ; Biomedical and Life Sciences ; Biomedicine ; Biotechnology ; Cell Membrane Permeability - drug effects ; Cornea - drug effects ; Cornea - metabolism ; Cornea - pathology ; Diffusion ; In Vitro Techniques ; Pharmacology/Toxicology ; Pharmacy ; Propranolol - metabolism ; Rabbits ; Rapid Communication ; Solubility</subject><ispartof>AAPS PharmSciTech, 2015-10, Vol.16 (5), p.1220-1226</ispartof><rights>American Association of Pharmaceutical Scientists 2015</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c442t-8db2da7158420a51abdc28c41863cbe65f41bef3b1a88c70c6ec4b5737bfdfaa3</citedby><cites>FETCH-LOGICAL-c442t-8db2da7158420a51abdc28c41863cbe65f41bef3b1a88c70c6ec4b5737bfdfaa3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4674647/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4674647/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,41488,42557,51319,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25771740$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Adelli, Goutham R.</creatorcontrib><creatorcontrib>Balguri, Sai Prachetan</creatorcontrib><creatorcontrib>Majumdar, Soumyajit</creatorcontrib><title>Effect of Cyclodextrins on Morphology and Barrier Characteristics of Isolated Rabbit Corneas</title><title>AAPS PharmSciTech</title><addtitle>AAPS PharmSciTech</addtitle><addtitle>AAPS PharmSciTech</addtitle><description>The objective of the present study is to investigate the confounding effects, if any, of beta-cyclodextrins (βCDs) on corneal permeability coefficients obtained from
in vitro
transmembrane diffusion studies. Transcorneal permeability studies were carried out with 2-hydroxypropyl-beta-cyclodextrin (HPβCD) and randomly methylated-beta-cyclodextrin (RMβCD) at 5 and 2.5%
w
/
v
in isotonic phosphate-buffered solution (IPBS) (pH 7.4). Rabbit corneas received from Pel-Freez Biologicals® were used for these studies. Propranolol hydrochloride (PHCl) (1 mg/mL) was used as the paracellular permeability marker. A series of permeation studies were carried out with IPBS as the control, with CDs on the donor side only, CDs on the receiver side only, and CDs on both the donor and receiver sides. At the end of 1 or 3 h, corneas were collected and fixed using a solution containing 2%
v
/
v
glutaraldehyde + 2%
w
/
v
paraformaldehyde + IPBS and histological examinations were performed (Excalibur Pathology, Inc). The order of transcorneal permeability of PHCl was found to be CDs on the receiver side > control (no CDs) ≈ CDs on both the receiver and donor sides > CDs on the donor side. Histology studies revealed that the corneal epithelial and endothelial layers remained intact in the control sets. Damage to the cornea was observed in the order of CDs on the receiver side > CDs on the donor side > CDs on both sides > control. The use of CDs in solutions for
in vitro
permeation experiments with rabbit corneas needs to be carefully considered to avoid confounding effects in the data obtained.</description><subject>2-Hydroxypropyl-beta-cyclodextrin</subject><subject>Animals</subject><subject>beta-Cyclodextrins - pharmacology</subject><subject>beta-Cyclodextrins - toxicity</subject><subject>Biochemistry</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Biotechnology</subject><subject>Cell Membrane Permeability - drug effects</subject><subject>Cornea - drug effects</subject><subject>Cornea - metabolism</subject><subject>Cornea - pathology</subject><subject>Diffusion</subject><subject>In Vitro Techniques</subject><subject>Pharmacology/Toxicology</subject><subject>Pharmacy</subject><subject>Propranolol - metabolism</subject><subject>Rabbits</subject><subject>Rapid Communication</subject><subject>Solubility</subject><issn>1530-9932</issn><issn>1530-9932</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kcuKFDEUhoMozkUfwI1k6aY0t6pUbQQtRmdgRBDdCeEkddKdoTppk_Rgz9NbTY_DuHGTBP7LOeQj5BVnb7lg_bvChVBDw3jbMLkcd0_IKW8la4ZBiqeP3ifkrJQbxoTkg3xOTkSrNdeKnZKfF96jqzR5Ou7dnCb8XXOIhaZIv6S8Xac5rfYU4kQ_Qs4BMx3XkMFVzKHU4MohelXSDBUn-g2sDZWOKUeE8oI88zAXfHl_n5Mfny6-j5fN9dfPV-OH68YpJWrTT1ZMoHnbK8Gg5WAnJ3qneN9JZ7FrveIWvbQc-t5p5jp0yrZaausnDyDPyftj73ZnNzg5jDXDbLY5bCDvTYJg_lViWJtVujWq06pTeil4c1-Q068dlmo2oTicZ4iYdsVwrQcmhFZysfKj1eVUSkb_MIYzc6BijlTMQsUcqJi7JfP68X4Pib8YFoM4GsoixRVmc5N2OS5_9p_WPwbgm1Q</recordid><startdate>20151001</startdate><enddate>20151001</enddate><creator>Adelli, Goutham R.</creator><creator>Balguri, Sai Prachetan</creator><creator>Majumdar, Soumyajit</creator><general>Springer US</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20151001</creationdate><title>Effect of Cyclodextrins on Morphology and Barrier Characteristics of Isolated Rabbit Corneas</title><author>Adelli, Goutham R. ; Balguri, Sai Prachetan ; Majumdar, Soumyajit</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c442t-8db2da7158420a51abdc28c41863cbe65f41bef3b1a88c70c6ec4b5737bfdfaa3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>2-Hydroxypropyl-beta-cyclodextrin</topic><topic>Animals</topic><topic>beta-Cyclodextrins - pharmacology</topic><topic>beta-Cyclodextrins - toxicity</topic><topic>Biochemistry</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>Biotechnology</topic><topic>Cell Membrane Permeability - drug effects</topic><topic>Cornea - drug effects</topic><topic>Cornea - metabolism</topic><topic>Cornea - pathology</topic><topic>Diffusion</topic><topic>In Vitro Techniques</topic><topic>Pharmacology/Toxicology</topic><topic>Pharmacy</topic><topic>Propranolol - metabolism</topic><topic>Rabbits</topic><topic>Rapid Communication</topic><topic>Solubility</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Adelli, Goutham R.</creatorcontrib><creatorcontrib>Balguri, Sai Prachetan</creatorcontrib><creatorcontrib>Majumdar, Soumyajit</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>AAPS PharmSciTech</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Adelli, Goutham R.</au><au>Balguri, Sai Prachetan</au><au>Majumdar, Soumyajit</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of Cyclodextrins on Morphology and Barrier Characteristics of Isolated Rabbit Corneas</atitle><jtitle>AAPS PharmSciTech</jtitle><stitle>AAPS PharmSciTech</stitle><addtitle>AAPS PharmSciTech</addtitle><date>2015-10-01</date><risdate>2015</risdate><volume>16</volume><issue>5</issue><spage>1220</spage><epage>1226</epage><pages>1220-1226</pages><issn>1530-9932</issn><eissn>1530-9932</eissn><abstract>The objective of the present study is to investigate the confounding effects, if any, of beta-cyclodextrins (βCDs) on corneal permeability coefficients obtained from
in vitro
transmembrane diffusion studies. Transcorneal permeability studies were carried out with 2-hydroxypropyl-beta-cyclodextrin (HPβCD) and randomly methylated-beta-cyclodextrin (RMβCD) at 5 and 2.5%
w
/
v
in isotonic phosphate-buffered solution (IPBS) (pH 7.4). Rabbit corneas received from Pel-Freez Biologicals® were used for these studies. Propranolol hydrochloride (PHCl) (1 mg/mL) was used as the paracellular permeability marker. A series of permeation studies were carried out with IPBS as the control, with CDs on the donor side only, CDs on the receiver side only, and CDs on both the donor and receiver sides. At the end of 1 or 3 h, corneas were collected and fixed using a solution containing 2%
v
/
v
glutaraldehyde + 2%
w
/
v
paraformaldehyde + IPBS and histological examinations were performed (Excalibur Pathology, Inc). The order of transcorneal permeability of PHCl was found to be CDs on the receiver side > control (no CDs) ≈ CDs on both the receiver and donor sides > CDs on the donor side. Histology studies revealed that the corneal epithelial and endothelial layers remained intact in the control sets. Damage to the cornea was observed in the order of CDs on the receiver side > CDs on the donor side > CDs on both sides > control. The use of CDs in solutions for
in vitro
permeation experiments with rabbit corneas needs to be carefully considered to avoid confounding effects in the data obtained.</abstract><cop>New York</cop><pub>Springer US</pub><pmid>25771740</pmid><doi>10.1208/s12249-015-0315-z</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; SpringerNature Journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | 2-Hydroxypropyl-beta-cyclodextrin Animals beta-Cyclodextrins - pharmacology beta-Cyclodextrins - toxicity Biochemistry Biomedical and Life Sciences Biomedicine Biotechnology Cell Membrane Permeability - drug effects Cornea - drug effects Cornea - metabolism Cornea - pathology Diffusion In Vitro Techniques Pharmacology/Toxicology Pharmacy Propranolol - metabolism Rabbits Rapid Communication Solubility |
| title | Effect of Cyclodextrins on Morphology and Barrier Characteristics of Isolated Rabbit Corneas |
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