On the Directional Specificity of Ribosome Frameshifting at a "Hungry" Codon
Limitation for aminoacyl-tRNA promotes ribosome frameshifting at certain sites. We have previously demonstrated ribosome frameshifting to the right (3') at an AAG site in one context, and to the left (5') at an AAG site in a different context. Here, we demonstrate that the "rightwing&...
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| Veröffentlicht in: | Proceedings of the National Academy of Sciences - PNAS 1993-06, Vol.90 (12), p.5469-5473 |
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| creator | Lindsley, Dale Gallant, Jonathan |
| description | Limitation for aminoacyl-tRNA promotes ribosome frameshifting at certain sites. We have previously demonstrated ribosome frameshifting to the right (3') at an AAG site in one context, and to the left (5') at an AAG site in a different context. Here, we demonstrate that the "rightwing" context is largely specific for frameshifting to the right, and the "leftwing" context is largely specific for frameshifting to the left. Analysis of these context rules, and the conversion of a sequence that promotes leftward frameshifting to one that promotes rightward frameshifting, demonstrated here, permits us to define a minimal heptanucleotide sequence sufficient for shiftiness in each direction at an AAG codon whose lysyl-tRNA is in short supply. |
| doi_str_mv | 10.1073/pnas.90.12.5469 |
| format | Article |
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We have previously demonstrated ribosome frameshifting to the right (3') at an AAG site in one context, and to the left (5') at an AAG site in a different context. Here, we demonstrate that the "rightwing" context is largely specific for frameshifting to the right, and the "leftwing" context is largely specific for frameshifting to the left. Analysis of these context rules, and the conversion of a sequence that promotes leftward frameshifting to one that promotes rightward frameshifting, demonstrated here, permits us to define a minimal heptanucleotide sequence sufficient for shiftiness in each direction at an AAG codon whose lysyl-tRNA is in short supply.</description><identifier>ISSN: 0027-8424</identifier><identifier>EISSN: 1091-6490</identifier><identifier>DOI: 10.1073/pnas.90.12.5469</identifier><identifier>PMID: 8516288</identifier><identifier>CODEN: PNASA6</identifier><language>eng</language><publisher>Washington, DC: National Academy of Sciences of the United States of America</publisher><subject>Amino Acid Sequence ; Amino acids ; Base Sequence ; beta-Galactosidase - biosynthesis ; beta-Galactosidase - genetics ; Biological and medical sciences ; Codon - genetics ; Codons ; Enzymes ; Escherichia coli ; Escherichia coli - enzymology ; Escherichia coli - genetics ; Frameshift Mutation ; Fundamental and applied biological sciences. Psychology ; Gene Deletion ; Genes ; Genes, Bacterial ; Genetics ; Kinetics ; Molecular and cellular biology ; Molecular genetics ; Molecular Sequence Data ; Oligodeoxyribonucleotides ; Oligonucleotides ; Open reading frames ; Plasmids ; Protein sequence analysis ; Reading frames ; Ribonucleic acid ; Ribosomes ; Ribosomes - metabolism ; RNA ; RNA, Transfer, Amino Acyl - genetics ; Transfer RNA ; Translation. Translation factors. Protein processing</subject><ispartof>Proceedings of the National Academy of Sciences - PNAS, 1993-06, Vol.90 (12), p.5469-5473</ispartof><rights>Copyright 1993 The National Academy of Sciences of the United States of America</rights><rights>1993 INIST-CNRS</rights><rights>Copyright National Academy of Sciences Jun 15, 1993</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c549t-1a094ae11099d5e8996aba1fdfc86c7521e6611463a415739851800f774d0c983</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.pnas.org/content/90/12.cover.gif</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/2362419$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/2362419$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,723,776,780,799,881,27903,27904,53770,53772,57996,58229</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4840342$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8516288$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lindsley, Dale</creatorcontrib><creatorcontrib>Gallant, Jonathan</creatorcontrib><title>On the Directional Specificity of Ribosome Frameshifting at a "Hungry" Codon</title><title>Proceedings of the National Academy of Sciences - PNAS</title><addtitle>Proc Natl Acad Sci U S A</addtitle><description>Limitation for aminoacyl-tRNA promotes ribosome frameshifting at certain sites. We have previously demonstrated ribosome frameshifting to the right (3') at an AAG site in one context, and to the left (5') at an AAG site in a different context. Here, we demonstrate that the "rightwing" context is largely specific for frameshifting to the right, and the "leftwing" context is largely specific for frameshifting to the left. Analysis of these context rules, and the conversion of a sequence that promotes leftward frameshifting to one that promotes rightward frameshifting, demonstrated here, permits us to define a minimal heptanucleotide sequence sufficient for shiftiness in each direction at an AAG codon whose lysyl-tRNA is in short supply.</description><subject>Amino Acid Sequence</subject><subject>Amino acids</subject><subject>Base Sequence</subject><subject>beta-Galactosidase - biosynthesis</subject><subject>beta-Galactosidase - genetics</subject><subject>Biological and medical sciences</subject><subject>Codon - genetics</subject><subject>Codons</subject><subject>Enzymes</subject><subject>Escherichia coli</subject><subject>Escherichia coli - enzymology</subject><subject>Escherichia coli - genetics</subject><subject>Frameshift Mutation</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Deletion</subject><subject>Genes</subject><subject>Genes, Bacterial</subject><subject>Genetics</subject><subject>Kinetics</subject><subject>Molecular and cellular biology</subject><subject>Molecular genetics</subject><subject>Molecular Sequence Data</subject><subject>Oligodeoxyribonucleotides</subject><subject>Oligonucleotides</subject><subject>Open reading frames</subject><subject>Plasmids</subject><subject>Protein sequence analysis</subject><subject>Reading frames</subject><subject>Ribonucleic acid</subject><subject>Ribosomes</subject><subject>Ribosomes - metabolism</subject><subject>RNA</subject><subject>RNA, Transfer, Amino Acyl - genetics</subject><subject>Transfer RNA</subject><subject>Translation. Translation factors. Protein processing</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1993</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkd2LEzEUxYMoa1199kUlFNGn6SaZTD7AF6muKxQW_HgOaSZpU2YmNcmI_e9N7VhcH_QpXM7v3px7DwBPMVpgxOur_aDTQpaCLBrK5D0ww0jiilGJ7oMZQoRXghL6EDxKaYcQko1AF-BCNJgRIWZgdTvAvLXwnY_WZB8G3cHPe2u888bnAwwOfvLrkEJv4XXUvU1b77IfNlBnqOH8Zhw28TCHy9CG4TF44HSX7JPpvQRfr99_Wd5Uq9sPH5dvV5VpqMwV1khSbXFxKtvGCimZXmvsWmcEM7wh2DKGMWW1prjhtSxuBUKOc9oiI0V9Cd6c5u7HdW9bY4ccdaf20fc6HlTQXt1VBr9Vm_BdUcYpKe2vpvYYvo02ZdX7ZGzX6cGGMSnecEEpw_8FMWsIF0wWcP4XuAtjLLdMiiBMOOWYFujqBJkYUorWnQ1jpI5hqmOYSpaCqGOYpeP5n3ue-Sm9or-cdJ2M7lzUg_HpjFFBUf1r3xcTdpz_W73zz-t_AsqNXZftj1zIZydyl3KIZ5TUjFAs65_9n8de</recordid><startdate>19930615</startdate><enddate>19930615</enddate><creator>Lindsley, Dale</creator><creator>Gallant, Jonathan</creator><general>National Academy of Sciences of the United States of America</general><general>National Acad Sciences</general><general>National Academy of Sciences</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QG</scope><scope>7QL</scope><scope>7QP</scope><scope>7QR</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TK</scope><scope>7TM</scope><scope>7TO</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19930615</creationdate><title>On the Directional Specificity of Ribosome Frameshifting at a "Hungry" Codon</title><author>Lindsley, Dale ; Gallant, Jonathan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c549t-1a094ae11099d5e8996aba1fdfc86c7521e6611463a415739851800f774d0c983</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1993</creationdate><topic>Amino Acid Sequence</topic><topic>Amino acids</topic><topic>Base Sequence</topic><topic>beta-Galactosidase - biosynthesis</topic><topic>beta-Galactosidase - genetics</topic><topic>Biological and medical sciences</topic><topic>Codon - genetics</topic><topic>Codons</topic><topic>Enzymes</topic><topic>Escherichia coli</topic><topic>Escherichia coli - enzymology</topic><topic>Escherichia coli - genetics</topic><topic>Frameshift Mutation</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Deletion</topic><topic>Genes</topic><topic>Genes, Bacterial</topic><topic>Genetics</topic><topic>Kinetics</topic><topic>Molecular and cellular biology</topic><topic>Molecular genetics</topic><topic>Molecular Sequence Data</topic><topic>Oligodeoxyribonucleotides</topic><topic>Oligonucleotides</topic><topic>Open reading frames</topic><topic>Plasmids</topic><topic>Protein sequence analysis</topic><topic>Reading frames</topic><topic>Ribonucleic acid</topic><topic>Ribosomes</topic><topic>Ribosomes - metabolism</topic><topic>RNA</topic><topic>RNA, Transfer, Amino Acyl - genetics</topic><topic>Transfer RNA</topic><topic>Translation. Translation factors. Protein processing</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lindsley, Dale</creatorcontrib><creatorcontrib>Gallant, Jonathan</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lindsley, Dale</au><au>Gallant, Jonathan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>On the Directional Specificity of Ribosome Frameshifting at a "Hungry" Codon</atitle><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle><addtitle>Proc Natl Acad Sci U S A</addtitle><date>1993-06-15</date><risdate>1993</risdate><volume>90</volume><issue>12</issue><spage>5469</spage><epage>5473</epage><pages>5469-5473</pages><issn>0027-8424</issn><eissn>1091-6490</eissn><coden>PNASA6</coden><abstract>Limitation for aminoacyl-tRNA promotes ribosome frameshifting at certain sites. We have previously demonstrated ribosome frameshifting to the right (3') at an AAG site in one context, and to the left (5') at an AAG site in a different context. Here, we demonstrate that the "rightwing" context is largely specific for frameshifting to the right, and the "leftwing" context is largely specific for frameshifting to the left. Analysis of these context rules, and the conversion of a sequence that promotes leftward frameshifting to one that promotes rightward frameshifting, demonstrated here, permits us to define a minimal heptanucleotide sequence sufficient for shiftiness in each direction at an AAG codon whose lysyl-tRNA is in short supply.</abstract><cop>Washington, DC</cop><pub>National Academy of Sciences of the United States of America</pub><pmid>8516288</pmid><doi>10.1073/pnas.90.12.5469</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Amino Acid Sequence Amino acids Base Sequence beta-Galactosidase - biosynthesis beta-Galactosidase - genetics Biological and medical sciences Codon - genetics Codons Enzymes Escherichia coli Escherichia coli - enzymology Escherichia coli - genetics Frameshift Mutation Fundamental and applied biological sciences. Psychology Gene Deletion Genes Genes, Bacterial Genetics Kinetics Molecular and cellular biology Molecular genetics Molecular Sequence Data Oligodeoxyribonucleotides Oligonucleotides Open reading frames Plasmids Protein sequence analysis Reading frames Ribonucleic acid Ribosomes Ribosomes - metabolism RNA RNA, Transfer, Amino Acyl - genetics Transfer RNA Translation. Translation factors. Protein processing |
| title | On the Directional Specificity of Ribosome Frameshifting at a "Hungry" Codon |
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