Circulating Microparticles Alter Formation, Structure and Properties of Fibrin Clots
Despite the importance of circulating microparticles in haemostasis and thrombosis, there is limited evidence for potential causative effects of naturally produced cell-derived microparticles on fibrin clot formation and its properties. We studied the significance of blood microparticles for fibrin...
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| Veröffentlicht in: | Scientific reports 2015-12, Vol.5 (1), p.17611-17611, Article 17611 |
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| description | Despite the importance of circulating microparticles in haemostasis and thrombosis, there is limited evidence for potential causative effects of naturally produced cell-derived microparticles on fibrin clot formation and its properties. We studied the significance of blood microparticles for fibrin formation, structure and susceptibility to fibrinolysis by removing them from platelet-free plasma using filtration. Clots made in platelet-free and microparticle-depleted plasma samples from the same healthy donors were analyzed in parallel. Microparticles accelerate fibrin polymerisation and support formation of more compact clots that resist internal and external fibrinolysis. These variations correlate with faster thrombin generation, suggesting thrombin-mediated kinetic effects of microparticles on fibrin formation, structure and properties. In addition, clots formed in the presence of microparticles, unlike clots from the microparticle-depleted plasma, contain 0.1–0.5-μm size granular and CD61-positive material on fibres, suggesting that platelet-derived microparticles attach to fibrin. Therefore, the blood of healthy individuals contains functional microparticles at the levels that have a procoagulant potential. They affect the structure and stability of fibrin clots indirectly through acceleration of thrombin generation and through direct physical incorporation into the fibrin network. Both mechanisms underlie a potential role of microparticles in haemostasis and thrombosis as modulators of fibrin formation, structure and resistance to fibrinolysis. |
| doi_str_mv | 10.1038/srep17611 |
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We studied the significance of blood microparticles for fibrin formation, structure and susceptibility to fibrinolysis by removing them from platelet-free plasma using filtration. Clots made in platelet-free and microparticle-depleted plasma samples from the same healthy donors were analyzed in parallel. Microparticles accelerate fibrin polymerisation and support formation of more compact clots that resist internal and external fibrinolysis. These variations correlate with faster thrombin generation, suggesting thrombin-mediated kinetic effects of microparticles on fibrin formation, structure and properties. In addition, clots formed in the presence of microparticles, unlike clots from the microparticle-depleted plasma, contain 0.1–0.5-μm size granular and CD61-positive material on fibres, suggesting that platelet-derived microparticles attach to fibrin. Therefore, the blood of healthy individuals contains functional microparticles at the levels that have a procoagulant potential. They affect the structure and stability of fibrin clots indirectly through acceleration of thrombin generation and through direct physical incorporation into the fibrin network. Both mechanisms underlie a potential role of microparticles in haemostasis and thrombosis as modulators of fibrin formation, structure and resistance to fibrinolysis.</description><identifier>ISSN: 2045-2322</identifier><identifier>EISSN: 2045-2322</identifier><identifier>DOI: 10.1038/srep17611</identifier><identifier>PMID: 26635081</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>101/28 ; 631/45/612/1221 ; 631/535/1258 ; 82 ; Adult ; Blood ; Blood Coagulation ; Blood platelets ; Blood Platelets - chemistry ; Blood Platelets - metabolism ; Cell-Derived Microparticles - metabolism ; Cell-Derived Microparticles - ultrastructure ; Female ; Fibers ; Fibrin ; Fibrin - chemistry ; Fibrin - metabolism ; Fibrinolysis ; Flow Cytometry ; Humanities and Social Sciences ; Humans ; Male ; Microparticles ; multidisciplinary ; Science ; Thrombin ; Thrombin - metabolism ; Thromboembolism ; Thrombosis ; Thrombosis - blood ; Thrombosis - metabolism ; Thrombosis - pathology</subject><ispartof>Scientific reports, 2015-12, Vol.5 (1), p.17611-17611, Article 17611</ispartof><rights>The Author(s) 2015</rights><rights>Copyright Nature Publishing Group Dec 2015</rights><rights>Copyright © 2015, Macmillan Publishers Limited 2015 Macmillan Publishers Limited</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c504t-cf5e608e2ab7786a5a053c40564cdb8227c1130a1aa4d6d35010ebcbd20384443</citedby><cites>FETCH-LOGICAL-c504t-cf5e608e2ab7786a5a053c40564cdb8227c1130a1aa4d6d35010ebcbd20384443</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4669434/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4669434/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,27901,27902,41096,42165,51551,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26635081$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zubairova, Laily D.</creatorcontrib><creatorcontrib>Nabiullina, Roza M.</creatorcontrib><creatorcontrib>Nagaswami, Chandrasekaran</creatorcontrib><creatorcontrib>Zuev, Yuriy F.</creatorcontrib><creatorcontrib>Mustafin, Ilshat G.</creatorcontrib><creatorcontrib>Litvinov, Rustem I.</creatorcontrib><creatorcontrib>Weisel, John W.</creatorcontrib><title>Circulating Microparticles Alter Formation, Structure and Properties of Fibrin Clots</title><title>Scientific reports</title><addtitle>Sci Rep</addtitle><addtitle>Sci Rep</addtitle><description>Despite the importance of circulating microparticles in haemostasis and thrombosis, there is limited evidence for potential causative effects of naturally produced cell-derived microparticles on fibrin clot formation and its properties. We studied the significance of blood microparticles for fibrin formation, structure and susceptibility to fibrinolysis by removing them from platelet-free plasma using filtration. Clots made in platelet-free and microparticle-depleted plasma samples from the same healthy donors were analyzed in parallel. Microparticles accelerate fibrin polymerisation and support formation of more compact clots that resist internal and external fibrinolysis. These variations correlate with faster thrombin generation, suggesting thrombin-mediated kinetic effects of microparticles on fibrin formation, structure and properties. In addition, clots formed in the presence of microparticles, unlike clots from the microparticle-depleted plasma, contain 0.1–0.5-μm size granular and CD61-positive material on fibres, suggesting that platelet-derived microparticles attach to fibrin. Therefore, the blood of healthy individuals contains functional microparticles at the levels that have a procoagulant potential. They affect the structure and stability of fibrin clots indirectly through acceleration of thrombin generation and through direct physical incorporation into the fibrin network. Both mechanisms underlie a potential role of microparticles in haemostasis and thrombosis as modulators of fibrin formation, structure and resistance to fibrinolysis.</description><subject>101/28</subject><subject>631/45/612/1221</subject><subject>631/535/1258</subject><subject>82</subject><subject>Adult</subject><subject>Blood</subject><subject>Blood Coagulation</subject><subject>Blood platelets</subject><subject>Blood Platelets - chemistry</subject><subject>Blood Platelets - metabolism</subject><subject>Cell-Derived Microparticles - metabolism</subject><subject>Cell-Derived Microparticles - ultrastructure</subject><subject>Female</subject><subject>Fibers</subject><subject>Fibrin</subject><subject>Fibrin - chemistry</subject><subject>Fibrin - metabolism</subject><subject>Fibrinolysis</subject><subject>Flow Cytometry</subject><subject>Humanities and Social Sciences</subject><subject>Humans</subject><subject>Male</subject><subject>Microparticles</subject><subject>multidisciplinary</subject><subject>Science</subject><subject>Thrombin</subject><subject>Thrombin - metabolism</subject><subject>Thromboembolism</subject><subject>Thrombosis</subject><subject>Thrombosis - blood</subject><subject>Thrombosis - metabolism</subject><subject>Thrombosis - pathology</subject><issn>2045-2322</issn><issn>2045-2322</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>C6C</sourceid><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNplkVFLwzAUhYMoTuYe_ANS8EXFapImafsijOJUmCg4n0OaZjOjS2aSCv57MzbH1LwkcD5Ozr0HgBMErxHMihvv1BLlDKE9cIQhoSnOMN7feffAwPs5jIfikqDyEPQwYxmFBToCk0o72bUiaDNLnrR0dilc0LJVPhm2QblkZN0iytZcJa_BdTJ0TiXCNMlLZFVkI2mnyUjXTpukam3wx-BgKlqvBpu7D95Gd5PqIR0_3z9Ww3EqKSQhlVOqGCwUFnWeF0xQAWkmCaSMyKYuMM4lQhkUSAjSsCYmRlDVsm5wnJsQkvXB7dp32dUL1UhlghMtXzq9EO6LW6H5b8Xodz6zn5wwVpJsZXC-MXD2o1M-8IX2UrWtMMp2nqOcRBTmBY3o2R90bjtn4ngcFWVZ0BLRMlIXayou0sdmptswCPJVXXxbV2RPd9NvyZ9yInC5BnyUzEy5nS__uX0DAIme8Q</recordid><startdate>20151204</startdate><enddate>20151204</enddate><creator>Zubairova, Laily D.</creator><creator>Nabiullina, Roza M.</creator><creator>Nagaswami, Chandrasekaran</creator><creator>Zuev, Yuriy F.</creator><creator>Mustafin, Ilshat G.</creator><creator>Litvinov, Rustem I.</creator><creator>Weisel, John W.</creator><general>Nature Publishing Group UK</general><general>Nature Publishing Group</general><scope>C6C</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20151204</creationdate><title>Circulating Microparticles Alter Formation, Structure and Properties of Fibrin Clots</title><author>Zubairova, Laily D. ; Nabiullina, Roza M. ; Nagaswami, Chandrasekaran ; Zuev, Yuriy F. ; Mustafin, Ilshat G. ; Litvinov, Rustem I. ; Weisel, John W.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c504t-cf5e608e2ab7786a5a053c40564cdb8227c1130a1aa4d6d35010ebcbd20384443</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>101/28</topic><topic>631/45/612/1221</topic><topic>631/535/1258</topic><topic>82</topic><topic>Adult</topic><topic>Blood</topic><topic>Blood Coagulation</topic><topic>Blood platelets</topic><topic>Blood Platelets - 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Scientific reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zubairova, Laily D.</au><au>Nabiullina, Roza M.</au><au>Nagaswami, Chandrasekaran</au><au>Zuev, Yuriy F.</au><au>Mustafin, Ilshat G.</au><au>Litvinov, Rustem I.</au><au>Weisel, John W.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Circulating Microparticles Alter Formation, Structure and Properties of Fibrin Clots</atitle><jtitle>Scientific reports</jtitle><stitle>Sci Rep</stitle><addtitle>Sci Rep</addtitle><date>2015-12-04</date><risdate>2015</risdate><volume>5</volume><issue>1</issue><spage>17611</spage><epage>17611</epage><pages>17611-17611</pages><artnum>17611</artnum><issn>2045-2322</issn><eissn>2045-2322</eissn><abstract>Despite the importance of circulating microparticles in haemostasis and thrombosis, there is limited evidence for potential causative effects of naturally produced cell-derived microparticles on fibrin clot formation and its properties. We studied the significance of blood microparticles for fibrin formation, structure and susceptibility to fibrinolysis by removing them from platelet-free plasma using filtration. Clots made in platelet-free and microparticle-depleted plasma samples from the same healthy donors were analyzed in parallel. Microparticles accelerate fibrin polymerisation and support formation of more compact clots that resist internal and external fibrinolysis. These variations correlate with faster thrombin generation, suggesting thrombin-mediated kinetic effects of microparticles on fibrin formation, structure and properties. In addition, clots formed in the presence of microparticles, unlike clots from the microparticle-depleted plasma, contain 0.1–0.5-μm size granular and CD61-positive material on fibres, suggesting that platelet-derived microparticles attach to fibrin. Therefore, the blood of healthy individuals contains functional microparticles at the levels that have a procoagulant potential. They affect the structure and stability of fibrin clots indirectly through acceleration of thrombin generation and through direct physical incorporation into the fibrin network. Both mechanisms underlie a potential role of microparticles in haemostasis and thrombosis as modulators of fibrin formation, structure and resistance to fibrinolysis.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>26635081</pmid><doi>10.1038/srep17611</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | 101/28 631/45/612/1221 631/535/1258 82 Adult Blood Blood Coagulation Blood platelets Blood Platelets - chemistry Blood Platelets - metabolism Cell-Derived Microparticles - metabolism Cell-Derived Microparticles - ultrastructure Female Fibers Fibrin Fibrin - chemistry Fibrin - metabolism Fibrinolysis Flow Cytometry Humanities and Social Sciences Humans Male Microparticles multidisciplinary Science Thrombin Thrombin - metabolism Thromboembolism Thrombosis Thrombosis - blood Thrombosis - metabolism Thrombosis - pathology |
| title | Circulating Microparticles Alter Formation, Structure and Properties of Fibrin Clots |
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