Oral operant ethanol self-administration in the absence of explicit cues, food restriction, water restriction and ethanol fading in C57BL/6J mice
Rationale Mouse models of ethanol (EtOH) self-administration are useful to identify genetic and biological underpinnings of alcohol use disorder. Objectives These experiments developed a novel method of oral operant EtOH self-administration in mice without explicitly paired cues, food/water restrict...
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description | Rationale
Mouse models of ethanol (EtOH) self-administration are useful to identify genetic and biological underpinnings of alcohol use disorder.
Objectives
These experiments developed a novel method of oral operant EtOH self-administration in mice without explicitly paired cues, food/water restriction, or EtOH fading.
Methods
Following magazine and lever training for 0.2 % saccharin (SAC), mice underwent nine weekly overnight sessions with lever pressing maintained by dipper presentation of 0, 3, 10, or 15 % EtOH in SAC or water vehicle. Ad libitum water was available from a bottle.
Results
Water vehicle mice ingested most fluid from the water bottle in contrast to SAC vehicle mice, which despite lever pressing demands, drank most of their fluid from the liquid dipper. Although EtOH in SAC vehicle mice showed concentration-dependent increases of g/kg EtOH intake, lever pressing decreased with increasing EtOH concentration and did not exceed that of SAC vehicle alone at any EtOH concentration. Mice reinforced with EtOH in water ingested less EtOH than mice reinforced with EtOH in SAC. EtOH in water mice, however, showed concentration-dependent increases in g/kg EtOH intake and lever presses. Fifteen percent EtOH in water mice showed significantly greater levels of lever pressing than water vehicle mice and a significant escalation of responding across weeks of exposure. Naltrexone pretreatment reduced EtOH self-administration and intake in these mice without altering responding in the vehicle control condition during the first hour of the session.
Conclusions
SAC facilitated EtOH intake but prevented observation of EtOH reinforcement. Water vehicle unmasked EtOH’s reinforcing effects. |
doi_str_mv | 10.1007/s00213-015-4040-9 |
format | Article |
fullrecord | <record><control><sourceid>gale_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_4667783</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A428126344</galeid><sourcerecordid>A428126344</sourcerecordid><originalsourceid>FETCH-LOGICAL-c640t-e6b3ead4849e7874b8e0cc4cebf8336da700c0a5ba68decccff45ff71e1c9b83</originalsourceid><addsrcrecordid>eNp1UstuEzEUtRCIhsAHsEGW2LDotNePGU82SCXiqUjddG95PNeJq4kd7EmBz-CP8SglpAjshaXr89C5OoS8ZHDBANRlBuBMVMDqSoKEavGIzJgUvOKg-GMyAxCiEqxuz8iznG-hHNnKp-SMN7xpmaxn5Od1MgONO0wmjBTHjQlxoBkHV5l-64PPYzKjj4H6QMcNUtNlDBZpdBS_7wZv_UjtHvM5dTH2NGEheDsxzuk3M2I6HVET-qOJM70P60l3Wat3q8vmC916i8_JE2eGjC_u3zm5-fD-ZvmpWl1__Ly8WlW2kTBW2HQCTV_yLFC1SnYtgrXSYudaIZreKAALpu5M0_ZorXVO1s4phswuulbMyduD7G7fbbG3GErQQe-S35r0Q0fj9cOf4Dd6He-0bBqlisWcvLkXSPFrWcCotz5bHAYTMO6zZopzVnBi8nr9F_Q27lMo6QqKASshgP9Brc2A2gcXi6-dRPWV5C3jjZCyoC7-gSq3x7K9GND5Mn9AYAeCTTHnhO6YkYGeaqQPNdKlRnqqkV4UzqvT5RwZv3tTAPwAyOUrrDGdJPqv6i9Nv9Q5</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1710148402</pqid></control><display><type>article</type><title>Oral operant ethanol self-administration in the absence of explicit cues, food restriction, water restriction and ethanol fading in C57BL/6J mice</title><source>MEDLINE</source><source>SpringerLink Journals - AutoHoldings</source><creator>Stafford, Alexandra M. ; Anderson, Shawn M. ; Shelton, Keith L. ; Brunzell, Darlene H.</creator><creatorcontrib>Stafford, Alexandra M. ; Anderson, Shawn M. ; Shelton, Keith L. ; Brunzell, Darlene H.</creatorcontrib><description>Rationale
Mouse models of ethanol (EtOH) self-administration are useful to identify genetic and biological underpinnings of alcohol use disorder.
Objectives
These experiments developed a novel method of oral operant EtOH self-administration in mice without explicitly paired cues, food/water restriction, or EtOH fading.
Methods
Following magazine and lever training for 0.2 % saccharin (SAC), mice underwent nine weekly overnight sessions with lever pressing maintained by dipper presentation of 0, 3, 10, or 15 % EtOH in SAC or water vehicle. Ad libitum water was available from a bottle.
Results
Water vehicle mice ingested most fluid from the water bottle in contrast to SAC vehicle mice, which despite lever pressing demands, drank most of their fluid from the liquid dipper. Although EtOH in SAC vehicle mice showed concentration-dependent increases of g/kg EtOH intake, lever pressing decreased with increasing EtOH concentration and did not exceed that of SAC vehicle alone at any EtOH concentration. Mice reinforced with EtOH in water ingested less EtOH than mice reinforced with EtOH in SAC. EtOH in water mice, however, showed concentration-dependent increases in g/kg EtOH intake and lever presses. Fifteen percent EtOH in water mice showed significantly greater levels of lever pressing than water vehicle mice and a significant escalation of responding across weeks of exposure. Naltrexone pretreatment reduced EtOH self-administration and intake in these mice without altering responding in the vehicle control condition during the first hour of the session.
Conclusions
SAC facilitated EtOH intake but prevented observation of EtOH reinforcement. Water vehicle unmasked EtOH’s reinforcing effects.</description><identifier>ISSN: 0033-3158</identifier><identifier>EISSN: 1432-2072</identifier><identifier>DOI: 10.1007/s00213-015-4040-9</identifier><identifier>PMID: 26268145</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Access control ; Administration, Oral ; Alcohol Drinking - prevention & control ; Alcohol Drinking - psychology ; Alcohol use ; Alcoholism ; Animals ; Biomedical and Life Sciences ; Biomedicine ; Conditioning, Operant - drug effects ; Conditioning, Operant - physiology ; Cues ; Ethanol ; Ethanol - administration & dosage ; Food Deprivation - physiology ; Food supply ; Genetic aspects ; Male ; Mice ; Mice, Inbred C57BL ; Naltrexone - administration & dosage ; Neurosciences ; Original Investigation ; Pharmacology/Toxicology ; Physiological aspects ; Psychiatry ; Psychopharmacology ; Reinforcement (Psychology) ; Rodents ; Saccharin - administration & dosage ; Self Administration ; Water - administration & dosage</subject><ispartof>Psychopharmacology, 2015-10, Vol.232 (20), p.3783-3795</ispartof><rights>Springer-Verlag Berlin Heidelberg 2015</rights><rights>COPYRIGHT 2015 Springer</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c640t-e6b3ead4849e7874b8e0cc4cebf8336da700c0a5ba68decccff45ff71e1c9b83</citedby><cites>FETCH-LOGICAL-c640t-e6b3ead4849e7874b8e0cc4cebf8336da700c0a5ba68decccff45ff71e1c9b83</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00213-015-4040-9$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00213-015-4040-9$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>230,314,780,784,885,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26268145$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Stafford, Alexandra M.</creatorcontrib><creatorcontrib>Anderson, Shawn M.</creatorcontrib><creatorcontrib>Shelton, Keith L.</creatorcontrib><creatorcontrib>Brunzell, Darlene H.</creatorcontrib><title>Oral operant ethanol self-administration in the absence of explicit cues, food restriction, water restriction and ethanol fading in C57BL/6J mice</title><title>Psychopharmacology</title><addtitle>Psychopharmacology</addtitle><addtitle>Psychopharmacology (Berl)</addtitle><description>Rationale
Mouse models of ethanol (EtOH) self-administration are useful to identify genetic and biological underpinnings of alcohol use disorder.
Objectives
These experiments developed a novel method of oral operant EtOH self-administration in mice without explicitly paired cues, food/water restriction, or EtOH fading.
Methods
Following magazine and lever training for 0.2 % saccharin (SAC), mice underwent nine weekly overnight sessions with lever pressing maintained by dipper presentation of 0, 3, 10, or 15 % EtOH in SAC or water vehicle. Ad libitum water was available from a bottle.
Results
Water vehicle mice ingested most fluid from the water bottle in contrast to SAC vehicle mice, which despite lever pressing demands, drank most of their fluid from the liquid dipper. Although EtOH in SAC vehicle mice showed concentration-dependent increases of g/kg EtOH intake, lever pressing decreased with increasing EtOH concentration and did not exceed that of SAC vehicle alone at any EtOH concentration. Mice reinforced with EtOH in water ingested less EtOH than mice reinforced with EtOH in SAC. EtOH in water mice, however, showed concentration-dependent increases in g/kg EtOH intake and lever presses. Fifteen percent EtOH in water mice showed significantly greater levels of lever pressing than water vehicle mice and a significant escalation of responding across weeks of exposure. Naltrexone pretreatment reduced EtOH self-administration and intake in these mice without altering responding in the vehicle control condition during the first hour of the session.
Conclusions
SAC facilitated EtOH intake but prevented observation of EtOH reinforcement. Water vehicle unmasked EtOH’s reinforcing effects.</description><subject>Access control</subject><subject>Administration, Oral</subject><subject>Alcohol Drinking - prevention & control</subject><subject>Alcohol Drinking - psychology</subject><subject>Alcohol use</subject><subject>Alcoholism</subject><subject>Animals</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Conditioning, Operant - drug effects</subject><subject>Conditioning, Operant - physiology</subject><subject>Cues</subject><subject>Ethanol</subject><subject>Ethanol - administration & dosage</subject><subject>Food Deprivation - physiology</subject><subject>Food supply</subject><subject>Genetic aspects</subject><subject>Male</subject><subject>Mice</subject><subject>Mice, Inbred C57BL</subject><subject>Naltrexone - administration & dosage</subject><subject>Neurosciences</subject><subject>Original Investigation</subject><subject>Pharmacology/Toxicology</subject><subject>Physiological aspects</subject><subject>Psychiatry</subject><subject>Psychopharmacology</subject><subject>Reinforcement (Psychology)</subject><subject>Rodents</subject><subject>Saccharin - administration & dosage</subject><subject>Self Administration</subject><subject>Water - administration & dosage</subject><issn>0033-3158</issn><issn>1432-2072</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp1UstuEzEUtRCIhsAHsEGW2LDotNePGU82SCXiqUjddG95PNeJq4kd7EmBz-CP8SglpAjshaXr89C5OoS8ZHDBANRlBuBMVMDqSoKEavGIzJgUvOKg-GMyAxCiEqxuz8iznG-hHNnKp-SMN7xpmaxn5Od1MgONO0wmjBTHjQlxoBkHV5l-64PPYzKjj4H6QMcNUtNlDBZpdBS_7wZv_UjtHvM5dTH2NGEheDsxzuk3M2I6HVET-qOJM70P60l3Wat3q8vmC916i8_JE2eGjC_u3zm5-fD-ZvmpWl1__Ly8WlW2kTBW2HQCTV_yLFC1SnYtgrXSYudaIZreKAALpu5M0_ZorXVO1s4phswuulbMyduD7G7fbbG3GErQQe-S35r0Q0fj9cOf4Dd6He-0bBqlisWcvLkXSPFrWcCotz5bHAYTMO6zZopzVnBi8nr9F_Q27lMo6QqKASshgP9Brc2A2gcXi6-dRPWV5C3jjZCyoC7-gSq3x7K9GND5Mn9AYAeCTTHnhO6YkYGeaqQPNdKlRnqqkV4UzqvT5RwZv3tTAPwAyOUrrDGdJPqv6i9Nv9Q5</recordid><startdate>20151001</startdate><enddate>20151001</enddate><creator>Stafford, Alexandra M.</creator><creator>Anderson, Shawn M.</creator><creator>Shelton, Keith L.</creator><creator>Brunzell, Darlene H.</creator><general>Springer Berlin Heidelberg</general><general>Springer</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QG</scope><scope>7QR</scope><scope>7RV</scope><scope>7TK</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>88G</scope><scope>8AO</scope><scope>8FD</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>K9.</scope><scope>KB0</scope><scope>M0S</scope><scope>M1P</scope><scope>M2M</scope><scope>NAPCQ</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PSYQQ</scope><scope>Q9U</scope><scope>5PM</scope></search><sort><creationdate>20151001</creationdate><title>Oral operant ethanol self-administration in the absence of explicit cues, food restriction, water restriction and ethanol fading in C57BL/6J mice</title><author>Stafford, Alexandra M. ; Anderson, Shawn M. ; Shelton, Keith L. ; Brunzell, Darlene H.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c640t-e6b3ead4849e7874b8e0cc4cebf8336da700c0a5ba68decccff45ff71e1c9b83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Access control</topic><topic>Administration, Oral</topic><topic>Alcohol Drinking - prevention & control</topic><topic>Alcohol Drinking - psychology</topic><topic>Alcohol use</topic><topic>Alcoholism</topic><topic>Animals</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>Conditioning, Operant - drug effects</topic><topic>Conditioning, Operant - physiology</topic><topic>Cues</topic><topic>Ethanol</topic><topic>Ethanol - administration & dosage</topic><topic>Food Deprivation - physiology</topic><topic>Food supply</topic><topic>Genetic aspects</topic><topic>Male</topic><topic>Mice</topic><topic>Mice, Inbred C57BL</topic><topic>Naltrexone - administration & dosage</topic><topic>Neurosciences</topic><topic>Original Investigation</topic><topic>Pharmacology/Toxicology</topic><topic>Physiological aspects</topic><topic>Psychiatry</topic><topic>Psychopharmacology</topic><topic>Reinforcement (Psychology)</topic><topic>Rodents</topic><topic>Saccharin - administration & dosage</topic><topic>Self Administration</topic><topic>Water - administration & dosage</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Stafford, Alexandra M.</creatorcontrib><creatorcontrib>Anderson, Shawn M.</creatorcontrib><creatorcontrib>Shelton, Keith L.</creatorcontrib><creatorcontrib>Brunzell, Darlene H.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Proquest Nursing & Allied Health Source</collection><collection>Neurosciences Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Psychology Database (Alumni)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Psychology Database</collection><collection>Nursing & Allied Health Premium</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest One Psychology</collection><collection>ProQuest Central Basic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Psychopharmacology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Stafford, Alexandra M.</au><au>Anderson, Shawn M.</au><au>Shelton, Keith L.</au><au>Brunzell, Darlene H.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Oral operant ethanol self-administration in the absence of explicit cues, food restriction, water restriction and ethanol fading in C57BL/6J mice</atitle><jtitle>Psychopharmacology</jtitle><stitle>Psychopharmacology</stitle><addtitle>Psychopharmacology (Berl)</addtitle><date>2015-10-01</date><risdate>2015</risdate><volume>232</volume><issue>20</issue><spage>3783</spage><epage>3795</epage><pages>3783-3795</pages><issn>0033-3158</issn><eissn>1432-2072</eissn><abstract>Rationale
Mouse models of ethanol (EtOH) self-administration are useful to identify genetic and biological underpinnings of alcohol use disorder.
Objectives
These experiments developed a novel method of oral operant EtOH self-administration in mice without explicitly paired cues, food/water restriction, or EtOH fading.
Methods
Following magazine and lever training for 0.2 % saccharin (SAC), mice underwent nine weekly overnight sessions with lever pressing maintained by dipper presentation of 0, 3, 10, or 15 % EtOH in SAC or water vehicle. Ad libitum water was available from a bottle.
Results
Water vehicle mice ingested most fluid from the water bottle in contrast to SAC vehicle mice, which despite lever pressing demands, drank most of their fluid from the liquid dipper. Although EtOH in SAC vehicle mice showed concentration-dependent increases of g/kg EtOH intake, lever pressing decreased with increasing EtOH concentration and did not exceed that of SAC vehicle alone at any EtOH concentration. Mice reinforced with EtOH in water ingested less EtOH than mice reinforced with EtOH in SAC. EtOH in water mice, however, showed concentration-dependent increases in g/kg EtOH intake and lever presses. Fifteen percent EtOH in water mice showed significantly greater levels of lever pressing than water vehicle mice and a significant escalation of responding across weeks of exposure. Naltrexone pretreatment reduced EtOH self-administration and intake in these mice without altering responding in the vehicle control condition during the first hour of the session.
Conclusions
SAC facilitated EtOH intake but prevented observation of EtOH reinforcement. Water vehicle unmasked EtOH’s reinforcing effects.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>26268145</pmid><doi>10.1007/s00213-015-4040-9</doi><tpages>13</tpages><oa>free_for_read</oa></addata></record> |
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source | MEDLINE; SpringerLink Journals - AutoHoldings |
subjects | Access control Administration, Oral Alcohol Drinking - prevention & control Alcohol Drinking - psychology Alcohol use Alcoholism Animals Biomedical and Life Sciences Biomedicine Conditioning, Operant - drug effects Conditioning, Operant - physiology Cues Ethanol Ethanol - administration & dosage Food Deprivation - physiology Food supply Genetic aspects Male Mice Mice, Inbred C57BL Naltrexone - administration & dosage Neurosciences Original Investigation Pharmacology/Toxicology Physiological aspects Psychiatry Psychopharmacology Reinforcement (Psychology) Rodents Saccharin - administration & dosage Self Administration Water - administration & dosage |
title | Oral operant ethanol self-administration in the absence of explicit cues, food restriction, water restriction and ethanol fading in C57BL/6J mice |
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