Function of desiccate in gustatory sensilla of drosophila melanogaster
Desiccate ( Desi ), initially discovered as a gene expressing in the epidermis of Drosophila larvae for protection from desiccation stress, was recently found to be robustly expressed in the adult labellum; however, the function, as well as precise expression sites, was unknown. Here, we found that...
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| Veröffentlicht in: | Scientific reports 2015-11, Vol.5 (1), p.17195-17195, Article 17195 |
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| creator | Kawano, Takeshi Ryuda, Masasuke Matsumoto, Hitoshi Ochiai, Masanori Oda, Yasunori Tanimura, Teiichi Csikos, Gyorge Moriya, Megumi Hayakawa, Yoichi |
| description | Desiccate
(
Desi
), initially discovered as a gene expressing in the epidermis of
Drosophila
larvae for protection from desiccation stress, was recently found to be robustly expressed in the adult labellum; however, the function, as well as precise expression sites, was unknown. Here, we found that
Desi
is expressed in two different types of non-neuronal cells of the labellum, the epidermis and thecogen accessory cells. Labellar
Desi
expression was significantly elevated under arid conditions, accompanied by an increase in water ingestion by adults.
Desi
overexpression also promoted water ingestion. In contrast, a knockdown of
Desi
expression reduced feeding as well as water ingestion due to a drastic decrease in the gustatory sensillar sensitivity for all tested tastants. These results indicate that
Desi
helps protect insects from desiccation damage by not only preventing dehydration through the integument but also accelerating water ingestion via elevated taste sensitivities of the sensilla. |
| doi_str_mv | 10.1038/srep17195 |
| format | Article |
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(
Desi
), initially discovered as a gene expressing in the epidermis of
Drosophila
larvae for protection from desiccation stress, was recently found to be robustly expressed in the adult labellum; however, the function, as well as precise expression sites, was unknown. Here, we found that
Desi
is expressed in two different types of non-neuronal cells of the labellum, the epidermis and thecogen accessory cells. Labellar
Desi
expression was significantly elevated under arid conditions, accompanied by an increase in water ingestion by adults.
Desi
overexpression also promoted water ingestion. In contrast, a knockdown of
Desi
expression reduced feeding as well as water ingestion due to a drastic decrease in the gustatory sensillar sensitivity for all tested tastants. These results indicate that
Desi
helps protect insects from desiccation damage by not only preventing dehydration through the integument but also accelerating water ingestion via elevated taste sensitivities of the sensilla.</description><identifier>ISSN: 2045-2322</identifier><identifier>EISSN: 2045-2322</identifier><identifier>DOI: 10.1038/srep17195</identifier><identifier>PMID: 26610608</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>38 ; 38/39 ; 42 ; 42/89 ; 631/337/505 ; 631/601/1737 ; Animals ; Antigen-presenting cells ; Chemoreceptor Cells - metabolism ; Chemoreceptor Cells - ultrastructure ; Dehydration ; Dehydration - genetics ; Dehydration - metabolism ; Desiccation ; Drinking - genetics ; Drosophila melanogaster - genetics ; Drosophila melanogaster - metabolism ; Drosophila Proteins - antagonists & inhibitors ; Drosophila Proteins - genetics ; Drosophila Proteins - metabolism ; Eating - genetics ; Epidermis ; Gene Expression Regulation ; Humanities and Social Sciences ; Ingestion ; Insects ; Integument ; Larva - cytology ; Larva - metabolism ; Larvae ; Male ; Membrane Proteins - antagonists & inhibitors ; Membrane Proteins - genetics ; Membrane Proteins - metabolism ; multidisciplinary ; RNA, Small Interfering - genetics ; RNA, Small Interfering - metabolism ; Science ; Sensilla ; Sensilla - metabolism ; Sensilla - ultrastructure ; Taste - genetics ; Taste receptors ; Water - metabolism</subject><ispartof>Scientific reports, 2015-11, Vol.5 (1), p.17195-17195, Article 17195</ispartof><rights>The Author(s) 2015</rights><rights>Copyright Nature Publishing Group Nov 2015</rights><rights>Copyright © 2015, Macmillan Publishers Limited 2015 Macmillan Publishers Limited</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c504t-db79c7f8cf2dfdc4477ad1c7e6e12ebde4f66e2896d8b004b346787ba7932c8d3</citedby><cites>FETCH-LOGICAL-c504t-db79c7f8cf2dfdc4477ad1c7e6e12ebde4f66e2896d8b004b346787ba7932c8d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4661605/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4661605/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,27923,27924,41119,42188,51575,53790,53792</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26610608$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kawano, Takeshi</creatorcontrib><creatorcontrib>Ryuda, Masasuke</creatorcontrib><creatorcontrib>Matsumoto, Hitoshi</creatorcontrib><creatorcontrib>Ochiai, Masanori</creatorcontrib><creatorcontrib>Oda, Yasunori</creatorcontrib><creatorcontrib>Tanimura, Teiichi</creatorcontrib><creatorcontrib>Csikos, Gyorge</creatorcontrib><creatorcontrib>Moriya, Megumi</creatorcontrib><creatorcontrib>Hayakawa, Yoichi</creatorcontrib><title>Function of desiccate in gustatory sensilla of drosophila melanogaster</title><title>Scientific reports</title><addtitle>Sci Rep</addtitle><addtitle>Sci Rep</addtitle><description>Desiccate
(
Desi
), initially discovered as a gene expressing in the epidermis of
Drosophila
larvae for protection from desiccation stress, was recently found to be robustly expressed in the adult labellum; however, the function, as well as precise expression sites, was unknown. Here, we found that
Desi
is expressed in two different types of non-neuronal cells of the labellum, the epidermis and thecogen accessory cells. Labellar
Desi
expression was significantly elevated under arid conditions, accompanied by an increase in water ingestion by adults.
Desi
overexpression also promoted water ingestion. In contrast, a knockdown of
Desi
expression reduced feeding as well as water ingestion due to a drastic decrease in the gustatory sensillar sensitivity for all tested tastants. These results indicate that
Desi
helps protect insects from desiccation damage by not only preventing dehydration through the integument but also accelerating water ingestion via elevated taste sensitivities of the sensilla.</description><subject>38</subject><subject>38/39</subject><subject>42</subject><subject>42/89</subject><subject>631/337/505</subject><subject>631/601/1737</subject><subject>Animals</subject><subject>Antigen-presenting cells</subject><subject>Chemoreceptor Cells - metabolism</subject><subject>Chemoreceptor Cells - ultrastructure</subject><subject>Dehydration</subject><subject>Dehydration - genetics</subject><subject>Dehydration - metabolism</subject><subject>Desiccation</subject><subject>Drinking - genetics</subject><subject>Drosophila melanogaster - genetics</subject><subject>Drosophila melanogaster - metabolism</subject><subject>Drosophila Proteins - antagonists & inhibitors</subject><subject>Drosophila Proteins - genetics</subject><subject>Drosophila Proteins - metabolism</subject><subject>Eating - 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metabolism</topic><topic>Chemoreceptor Cells - ultrastructure</topic><topic>Dehydration</topic><topic>Dehydration - genetics</topic><topic>Dehydration - metabolism</topic><topic>Desiccation</topic><topic>Drinking - genetics</topic><topic>Drosophila melanogaster - genetics</topic><topic>Drosophila melanogaster - metabolism</topic><topic>Drosophila Proteins - antagonists & inhibitors</topic><topic>Drosophila Proteins - genetics</topic><topic>Drosophila Proteins - metabolism</topic><topic>Eating - genetics</topic><topic>Epidermis</topic><topic>Gene Expression Regulation</topic><topic>Humanities and Social Sciences</topic><topic>Ingestion</topic><topic>Insects</topic><topic>Integument</topic><topic>Larva - cytology</topic><topic>Larva - metabolism</topic><topic>Larvae</topic><topic>Male</topic><topic>Membrane Proteins - antagonists & inhibitors</topic><topic>Membrane Proteins - genetics</topic><topic>Membrane Proteins - metabolism</topic><topic>multidisciplinary</topic><topic>RNA, Small Interfering - 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Scientific reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kawano, Takeshi</au><au>Ryuda, Masasuke</au><au>Matsumoto, Hitoshi</au><au>Ochiai, Masanori</au><au>Oda, Yasunori</au><au>Tanimura, Teiichi</au><au>Csikos, Gyorge</au><au>Moriya, Megumi</au><au>Hayakawa, Yoichi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Function of desiccate in gustatory sensilla of drosophila melanogaster</atitle><jtitle>Scientific reports</jtitle><stitle>Sci Rep</stitle><addtitle>Sci Rep</addtitle><date>2015-11-27</date><risdate>2015</risdate><volume>5</volume><issue>1</issue><spage>17195</spage><epage>17195</epage><pages>17195-17195</pages><artnum>17195</artnum><issn>2045-2322</issn><eissn>2045-2322</eissn><abstract>Desiccate
(
Desi
), initially discovered as a gene expressing in the epidermis of
Drosophila
larvae for protection from desiccation stress, was recently found to be robustly expressed in the adult labellum; however, the function, as well as precise expression sites, was unknown. Here, we found that
Desi
is expressed in two different types of non-neuronal cells of the labellum, the epidermis and thecogen accessory cells. Labellar
Desi
expression was significantly elevated under arid conditions, accompanied by an increase in water ingestion by adults.
Desi
overexpression also promoted water ingestion. In contrast, a knockdown of
Desi
expression reduced feeding as well as water ingestion due to a drastic decrease in the gustatory sensillar sensitivity for all tested tastants. These results indicate that
Desi
helps protect insects from desiccation damage by not only preventing dehydration through the integument but also accelerating water ingestion via elevated taste sensitivities of the sensilla.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>26610608</pmid><doi>10.1038/srep17195</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; DOAJ Directory of Open Access Journals; Springer Nature OA Free Journals; Nature Free; EZB-FREE-00999 freely available EZB journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | 38 38/39 42 42/89 631/337/505 631/601/1737 Animals Antigen-presenting cells Chemoreceptor Cells - metabolism Chemoreceptor Cells - ultrastructure Dehydration Dehydration - genetics Dehydration - metabolism Desiccation Drinking - genetics Drosophila melanogaster - genetics Drosophila melanogaster - metabolism Drosophila Proteins - antagonists & inhibitors Drosophila Proteins - genetics Drosophila Proteins - metabolism Eating - genetics Epidermis Gene Expression Regulation Humanities and Social Sciences Ingestion Insects Integument Larva - cytology Larva - metabolism Larvae Male Membrane Proteins - antagonists & inhibitors Membrane Proteins - genetics Membrane Proteins - metabolism multidisciplinary RNA, Small Interfering - genetics RNA, Small Interfering - metabolism Science Sensilla Sensilla - metabolism Sensilla - ultrastructure Taste - genetics Taste receptors Water - metabolism |
| title | Function of desiccate in gustatory sensilla of drosophila melanogaster |
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