Regulation of Synapse Structure and Function by the Drosophila Tumor Suppressor Gene dlg
Mutations of the tumor suppressor gene discs-large (dlg) lead to postsynaptic structural defects. Here, we report that mutations in dlg also result in larger synaptic currents at fly neuromuscular junctions. By selectively targeting DLG protein to either muscles or motorneurons using Gal-4 enhancer...
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| Veröffentlicht in: | Neuron (Cambridge, Mass.) Mass.), 1996-10, Vol.17 (4), p.627-640 |
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| creator | Budnik, Vivian Koh, Young-Ho Guan, Bo Hartmann, Beate Hough, Colleen Woods, Daniel Gorczyca, Michael |
| description | Mutations of the tumor suppressor gene
discs-large (dlg) lead to postsynaptic structural defects. Here, we report that mutations in
dlg also result in larger synaptic currents at fly neuromuscular junctions. By selectively targeting DLG protein to either muscles or motorneurons using
Gal-4 enhancer trap lines, we were able to rescue substantially the reduced postsynaptic structure in mutants. Rescue of the physiological defect was accomplished by presynaptic, but not postsynaptic targeting, consistent with our finding that miniature excitatory junctional currents were not changed in
dlg mutants. These results suggest that DLG functions in the regulation of neurotransmitter release and postsynaptic structure. We propose that DLG is an integral part of a mechanism by which changes in both neurotransmitter release and synapse structure are accomplished during development and plasticity. |
| doi_str_mv | 10.1016/S0896-6273(00)80196-8 |
| format | Article |
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discs-large (dlg) lead to postsynaptic structural defects. Here, we report that mutations in
dlg also result in larger synaptic currents at fly neuromuscular junctions. By selectively targeting DLG protein to either muscles or motorneurons using
Gal-4 enhancer trap lines, we were able to rescue substantially the reduced postsynaptic structure in mutants. Rescue of the physiological defect was accomplished by presynaptic, but not postsynaptic targeting, consistent with our finding that miniature excitatory junctional currents were not changed in
dlg mutants. These results suggest that DLG functions in the regulation of neurotransmitter release and postsynaptic structure. We propose that DLG is an integral part of a mechanism by which changes in both neurotransmitter release and synapse structure are accomplished during development and plasticity.</description><identifier>ISSN: 0896-6273</identifier><identifier>EISSN: 1097-4199</identifier><identifier>DOI: 10.1016/S0896-6273(00)80196-8</identifier><identifier>PMID: 8893021</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Animals ; Axons ; Drosophila - genetics ; Drosophila - physiology ; Drosophila Proteins ; Evoked Potentials ; Gene Expression Regulation, Developmental ; Genes, Insect ; Genes, Tumor Suppressor ; Insect Hormones - biosynthesis ; Insect Hormones - genetics ; Insecta ; Microscopy, Electron ; Motor Neurons - physiology ; Motor Neurons - ultrastructure ; Muscles - innervation ; Mutagenesis ; Neuromuscular Junction - physiology ; Neuromuscular Junction - ultrastructure ; Synapses - physiology ; Synapses - ultrastructure ; Synaptic Transmission ; Tumor Suppressor Proteins</subject><ispartof>Neuron (Cambridge, Mass.), 1996-10, Vol.17 (4), p.627-640</ispartof><rights>1996 Cell Press</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c611t-210a5cda3bda1a61830884d68ce36d0b81776f2a17b32e2ae0212e1e57d0c7f3</citedby><cites>FETCH-LOGICAL-c611t-210a5cda3bda1a61830884d68ce36d0b81776f2a17b32e2ae0212e1e57d0c7f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0896627300801968$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>230,314,776,780,881,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8893021$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Budnik, Vivian</creatorcontrib><creatorcontrib>Koh, Young-Ho</creatorcontrib><creatorcontrib>Guan, Bo</creatorcontrib><creatorcontrib>Hartmann, Beate</creatorcontrib><creatorcontrib>Hough, Colleen</creatorcontrib><creatorcontrib>Woods, Daniel</creatorcontrib><creatorcontrib>Gorczyca, Michael</creatorcontrib><title>Regulation of Synapse Structure and Function by the Drosophila Tumor Suppressor Gene dlg</title><title>Neuron (Cambridge, Mass.)</title><addtitle>Neuron</addtitle><description>Mutations of the tumor suppressor gene
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dlg also result in larger synaptic currents at fly neuromuscular junctions. By selectively targeting DLG protein to either muscles or motorneurons using
Gal-4 enhancer trap lines, we were able to rescue substantially the reduced postsynaptic structure in mutants. Rescue of the physiological defect was accomplished by presynaptic, but not postsynaptic targeting, consistent with our finding that miniature excitatory junctional currents were not changed in
dlg mutants. These results suggest that DLG functions in the regulation of neurotransmitter release and postsynaptic structure. We propose that DLG is an integral part of a mechanism by which changes in both neurotransmitter release and synapse structure are accomplished during development and plasticity.</description><subject>Animals</subject><subject>Axons</subject><subject>Drosophila - genetics</subject><subject>Drosophila - physiology</subject><subject>Drosophila Proteins</subject><subject>Evoked Potentials</subject><subject>Gene Expression Regulation, Developmental</subject><subject>Genes, Insect</subject><subject>Genes, Tumor Suppressor</subject><subject>Insect Hormones - biosynthesis</subject><subject>Insect Hormones - genetics</subject><subject>Insecta</subject><subject>Microscopy, Electron</subject><subject>Motor Neurons - physiology</subject><subject>Motor Neurons - ultrastructure</subject><subject>Muscles - innervation</subject><subject>Mutagenesis</subject><subject>Neuromuscular Junction - physiology</subject><subject>Neuromuscular Junction - ultrastructure</subject><subject>Synapses - physiology</subject><subject>Synapses - ultrastructure</subject><subject>Synaptic Transmission</subject><subject>Tumor Suppressor Proteins</subject><issn>0896-6273</issn><issn>1097-4199</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFUcFuEzEUtBCopIVPqOQTgsMWv92s7b2AUKEFqRISyYGb5bXfJkYbe7HXlfL3uEkUwYmTbc28mecZQq6B3QAD_n7FZMcrXovmLWPvJIPyks_IAlgnqiV03XOyOFNeksuUfjEGy7aDC3IhZdewGhbk5w_c5FHPLngaBrraez0lpKs5ZjPniFR7S--yNwdGv6fzFunnGFKYtm7UdJ13IdJVnqaIKZXrPXqkdty8Ii8GPSZ8fTqvyPruy_r2a_Xw_f7b7aeHynCAuaqB6dZY3fRWg-YgGybl0nJpsOGW9RKE4EOtQfRNjbXGsnWNgK2wzIihuSIfjrJT7ndoDfo56lFN0e103KugnfoX8W6rNuFRLXnxF7wIvDkJxPA7Y5rVziWD46g9hpwUtJKLkm0htkeiKb9PEYezCTD11Ig6NKKe4laMqUMjSpa56783PE-dKij4xyOOJaVHh1El49AbtC6imZUN7j8OfwD5fp0j</recordid><startdate>19961001</startdate><enddate>19961001</enddate><creator>Budnik, Vivian</creator><creator>Koh, Young-Ho</creator><creator>Guan, Bo</creator><creator>Hartmann, Beate</creator><creator>Hough, Colleen</creator><creator>Woods, Daniel</creator><creator>Gorczyca, Michael</creator><general>Elsevier Inc</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SS</scope><scope>7TK</scope><scope>5PM</scope></search><sort><creationdate>19961001</creationdate><title>Regulation of Synapse Structure and Function by the Drosophila Tumor Suppressor Gene dlg</title><author>Budnik, Vivian ; 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discs-large (dlg) lead to postsynaptic structural defects. Here, we report that mutations in
dlg also result in larger synaptic currents at fly neuromuscular junctions. By selectively targeting DLG protein to either muscles or motorneurons using
Gal-4 enhancer trap lines, we were able to rescue substantially the reduced postsynaptic structure in mutants. Rescue of the physiological defect was accomplished by presynaptic, but not postsynaptic targeting, consistent with our finding that miniature excitatory junctional currents were not changed in
dlg mutants. These results suggest that DLG functions in the regulation of neurotransmitter release and postsynaptic structure. We propose that DLG is an integral part of a mechanism by which changes in both neurotransmitter release and synapse structure are accomplished during development and plasticity.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>8893021</pmid><doi>10.1016/S0896-6273(00)80196-8</doi><tpages>14</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; Cell Press Free Archives; Elsevier ScienceDirect Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | Animals Axons Drosophila - genetics Drosophila - physiology Drosophila Proteins Evoked Potentials Gene Expression Regulation, Developmental Genes, Insect Genes, Tumor Suppressor Insect Hormones - biosynthesis Insect Hormones - genetics Insecta Microscopy, Electron Motor Neurons - physiology Motor Neurons - ultrastructure Muscles - innervation Mutagenesis Neuromuscular Junction - physiology Neuromuscular Junction - ultrastructure Synapses - physiology Synapses - ultrastructure Synaptic Transmission Tumor Suppressor Proteins |
| title | Regulation of Synapse Structure and Function by the Drosophila Tumor Suppressor Gene dlg |
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