Topiramate effects lipolysis in 3T3-L1 adipocytes

Studies have shown that topiramate (TPM)-induced weight loss can be dependent on the central nervous system (CNS). However, the direct action of TPM on adipose tissue has not been tested previously. Thus, the present study aimed to examine whether TPM modulates lipolysis in 3T3-L1. The 3T3-L1 cells...

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Veröffentlicht in:Biomedical reports 2015-11, Vol.3 (6), p.827-830
Hauptverfasser: MARTINS, GABRIELA POLTRONIERI CAMPAGNARO, SOUZA, CAMILA OLIVEIRA, MARQUES, SCHEROLIN, LUCIANO, THAIS FERNANDES, DA SILVA PIERI, BRUNO LUIZ, ROSA, JOSÉ CÉSAR, DA SILVA, ADELINO SANCHEZ RAMOS, PAULI, JOSÉ RODRIGO, CINTRA, DENNYS ESPER, ROPELLE, EDUARDO ROCHETE, RODRIGUES, BRUNO, DE LIRA, FABIO SANTOS, DE SOUZA, CLAUDIO TEODORO
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container_issue 6
container_start_page 827
container_title Biomedical reports
container_volume 3
creator MARTINS, GABRIELA POLTRONIERI CAMPAGNARO
SOUZA, CAMILA OLIVEIRA
MARQUES, SCHEROLIN
LUCIANO, THAIS FERNANDES
DA SILVA PIERI, BRUNO LUIZ
ROSA, JOSÉ CÉSAR
DA SILVA, ADELINO SANCHEZ RAMOS
PAULI, JOSÉ RODRIGO
CINTRA, DENNYS ESPER
ROPELLE, EDUARDO ROCHETE
RODRIGUES, BRUNO
DE LIRA, FABIO SANTOS
DE SOUZA, CLAUDIO TEODORO
description Studies have shown that topiramate (TPM)-induced weight loss can be dependent on the central nervous system (CNS). However, the direct action of TPM on adipose tissue has not been tested previously. Thus, the present study aimed to examine whether TPM modulates lipolysis in 3T3-L1. The 3T3-L1 cells were incubated in 50 µM TPM for 30 min. The β-adrenergic stimulator, isoproterenol, was used as a positive control. The release of lactate dehydrogenase, non-esterified fatty acid, glycerol and incorporation of 14C-palmitate to lipid were analyzed. The phosphorylation of protein kinase A (PKA), hormone-sensitive lipase (HSL), adipocyte triglyceride lipase (ATGL) and perilipin A, as well as the protein levels of comparative genetic identification 58 (CGI-58) were assessed. The levels of glycerol and non-esterified fatty acid increased markedly when the cells were treated with TPM. The TPM effects were similar to the isoproterenol positive control. Additionally, TPM reduced lipogenesis. These results were observed without any change in cell viability. Finally, the phosphorylation of PKA, HSL, ATGL and perilipin A, as well as the protein levels of CGI-58 were increased compared to the control cells. These results were similar to those observed in the cells treated with isoproterenol. The present results show that TPM increased the phosphorylation of pivotal lipolytic enzymes, which induced lipolysis in 3T3-L1 adipocytes, suggesting that this drug may act directly in the adipose tissue independent from its effect on the CNS.
doi_str_mv 10.3892/br.2015.514
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However, the direct action of TPM on adipose tissue has not been tested previously. Thus, the present study aimed to examine whether TPM modulates lipolysis in 3T3-L1. The 3T3-L1 cells were incubated in 50 µM TPM for 30 min. The β-adrenergic stimulator, isoproterenol, was used as a positive control. The release of lactate dehydrogenase, non-esterified fatty acid, glycerol and incorporation of 14C-palmitate to lipid were analyzed. The phosphorylation of protein kinase A (PKA), hormone-sensitive lipase (HSL), adipocyte triglyceride lipase (ATGL) and perilipin A, as well as the protein levels of comparative genetic identification 58 (CGI-58) were assessed. The levels of glycerol and non-esterified fatty acid increased markedly when the cells were treated with TPM. The TPM effects were similar to the isoproterenol positive control. Additionally, TPM reduced lipogenesis. These results were observed without any change in cell viability. Finally, the phosphorylation of PKA, HSL, ATGL and perilipin A, as well as the protein levels of CGI-58 were increased compared to the control cells. These results were similar to those observed in the cells treated with isoproterenol. The present results show that TPM increased the phosphorylation of pivotal lipolytic enzymes, which induced lipolysis in 3T3-L1 adipocytes, suggesting that this drug may act directly in the adipose tissue independent from its effect on the CNS.</abstract><cop>England</cop><pub>D.A. Spandidos</pub><pmid>26623024</pmid><doi>10.3892/br.2015.514</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record>
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source Spandidos Publications Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central
subjects 3T3-L1 adipocytes
Adipocytes
Adipose tissue
Central nervous system
Enzymes
Esterification
Fat cells
Fatty acids
Fluorides
Glycerol
Immunoglobulins
Insulin
Isoproterenol
Kinases
L-Lactate dehydrogenase
Lactate dehydrogenase
Lactic acid
Lipase
Lipids
Lipogenesis
Lipolysis
Membranes
Obesity
Palmitic acid
Phosphorylation
Physiological aspects
Protein kinase A
Proteins
Rodents
Software
Topiramate
Weight control
Weight loss
title Topiramate effects lipolysis in 3T3-L1 adipocytes
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