Efficacy of cleansing agents in killing microorganisms in mixed species biofilms present on silicone facial prostheses—an in vitro study
Objectives The purpose of this study was to assess the efficacy of different cleansing agents in killing mixed species biofilms on silicone facial prostheses. Materials and methods Two bacterial and three yeast strains, isolated from silicone facial prostheses, were selected for the mixed species bi...
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| Veröffentlicht in: | Clinical oral investigations 2015-12, Vol.19 (9), p.2285-2293 |
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| creator | Ariani, Nina Visser, Anita Teulings, Margot R. I. M. Dijk, Melissa Rahardjo, Tri Budi W. Vissink, Arjan van der Mei, Henny C. |
| description | Objectives
The purpose of this study was to assess the efficacy of different cleansing agents in killing mixed species biofilms on silicone facial prostheses.
Materials and methods
Two bacterial and three yeast strains, isolated from silicone facial prostheses, were selected for the mixed species biofilms. A variety of agents used to clean facial prostheses were employed, viz., antibacterial soap, essential-oil-containing mouth rinse, ethanol 27 %, chlorhexidine mouth rinse, and buttermilk. Colony forming units (CFUs) and live/dead staining were analyzed to assess the efficacy of these cleansing agents against 24-h and 2-week biofilms and regrown biofilms on silicone samples.
Results
Chlorhexidine was the most effective cleansing agent. Chlorhexidine killed 8 log unit CFUs (>99.99 % killing) in a 24-h biofilm and 5 log unit CFUs (>99.99 % killing) in 2-week biofilms. Also, after regrowth and repeated treatment of the biofilm, chlorhexidine was the most effective cleansing agent showing no detectable CFUs. The essential-oil-containing mouth rinse (containing 26.9 % ethanol) showed a similar efficacy as ethanol (27 %) alone. Antibacterial soap and buttermilk were the least effective agents tested.
Conclusions
Chlorhexidine showed the highest reduction in CFUs in 24-h, 2-week, and regrown mixed species biofilm of microorganisms isolated from silicone facial prostheses.
Clinical relevance
Chlorhexidine mouth rinse (easy obtainable and relatively cheap) is very effective in killing bacteria and yeast present in biofilms on silicone facial prostheses. When applied on a regular basis, cleansing a facial prosthesis with chlorhexidine will presumably increase its lifetime and reduce skin irritations. |
| doi_str_mv | 10.1007/s00784-015-1453-0 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_4656749</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1823943074</sourcerecordid><originalsourceid>FETCH-LOGICAL-c573t-483d3737704e009b7ae5828beef1061c6ff65d43a76f6b87b6845a60f2d79d303</originalsourceid><addsrcrecordid>eNqFkk1vFSEYhSfGxn7oD3BjSNy4GQvD18zGxDTVmjRxU9eEYWBKZeDKO9P07ly79hf6S2R6a1NNTDdAOA-H94VTVS8JfkswlsdQhpbVmPCaME5r_KQ6IIyKmkpJnt6um1p0LdmvDgGuMCZMSPqs2m94yyjm-KD6ceqcN9psUXLIBKsj-DgiPdo4A_IRffUhrDuTNzmlPOroYbpVJn9jBwQba7wF1PvkfCjKJlsoh1GKCHzwJkWLnDZehyIlmC-LDL--_9RxNbn2c04I5mXYPq_2nA5gX9zNR9WXD6cXJ2f1-eePn07en9eGSzrXrKUDlaVDzCzGXS-15W3T9tY6ggUxwjnBB0a1FE70rexFy7gW2DWD7AaK6VH1bue7WfrJDqYUm3VQm-wnnbcqaa_-VqK_VGO6VkxwIVlXDN7cGeT0bbEwq8mDsSHoaNMCirQN7cr7SvY4KqlghOGGF_T1P-hVWnIsL7FSnHeiY6JQZEeV3wDI1t3XTbBaQ6F2oVAlFGoNhVobfvWw4fsTf1JQgGYHQJHiaPODq__r-hvhucWH</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1735596946</pqid></control><display><type>article</type><title>Efficacy of cleansing agents in killing microorganisms in mixed species biofilms present on silicone facial prostheses—an in vitro study</title><source>MEDLINE</source><source>SpringerLink Journals - AutoHoldings</source><creator>Ariani, Nina ; Visser, Anita ; Teulings, Margot R. I. M. ; Dijk, Melissa ; Rahardjo, Tri Budi W. ; Vissink, Arjan ; van der Mei, Henny C.</creator><creatorcontrib>Ariani, Nina ; Visser, Anita ; Teulings, Margot R. I. M. ; Dijk, Melissa ; Rahardjo, Tri Budi W. ; Vissink, Arjan ; van der Mei, Henny C.</creatorcontrib><description>Objectives
The purpose of this study was to assess the efficacy of different cleansing agents in killing mixed species biofilms on silicone facial prostheses.
Materials and methods
Two bacterial and three yeast strains, isolated from silicone facial prostheses, were selected for the mixed species biofilms. A variety of agents used to clean facial prostheses were employed, viz., antibacterial soap, essential-oil-containing mouth rinse, ethanol 27 %, chlorhexidine mouth rinse, and buttermilk. Colony forming units (CFUs) and live/dead staining were analyzed to assess the efficacy of these cleansing agents against 24-h and 2-week biofilms and regrown biofilms on silicone samples.
Results
Chlorhexidine was the most effective cleansing agent. Chlorhexidine killed 8 log unit CFUs (>99.99 % killing) in a 24-h biofilm and 5 log unit CFUs (>99.99 % killing) in 2-week biofilms. Also, after regrowth and repeated treatment of the biofilm, chlorhexidine was the most effective cleansing agent showing no detectable CFUs. The essential-oil-containing mouth rinse (containing 26.9 % ethanol) showed a similar efficacy as ethanol (27 %) alone. Antibacterial soap and buttermilk were the least effective agents tested.
Conclusions
Chlorhexidine showed the highest reduction in CFUs in 24-h, 2-week, and regrown mixed species biofilm of microorganisms isolated from silicone facial prostheses.
Clinical relevance
Chlorhexidine mouth rinse (easy obtainable and relatively cheap) is very effective in killing bacteria and yeast present in biofilms on silicone facial prostheses. When applied on a regular basis, cleansing a facial prosthesis with chlorhexidine will presumably increase its lifetime and reduce skin irritations.</description><identifier>ISSN: 1432-6981</identifier><identifier>EISSN: 1436-3771</identifier><identifier>DOI: 10.1007/s00784-015-1453-0</identifier><identifier>PMID: 25843050</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Anti-Infective Agents, Local - pharmacology ; Biofilms - drug effects ; Buttermilk ; Candida - growth & development ; Chlorhexidine ; Dentistry ; Disinfectants - pharmacology ; Ethanol ; Face ; Humans ; Medicine ; Mouthwashes ; Oils, Volatile ; Original ; Original Article ; Prostheses and Implants ; Silicone Elastomers ; Soaps ; Staphylococcus - growth & development</subject><ispartof>Clinical oral investigations, 2015-12, Vol.19 (9), p.2285-2293</ispartof><rights>The Author(s) 2015</rights><rights>Springer-Verlag Berlin Heidelberg 2015</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c573t-483d3737704e009b7ae5828beef1061c6ff65d43a76f6b87b6845a60f2d79d303</citedby><cites>FETCH-LOGICAL-c573t-483d3737704e009b7ae5828beef1061c6ff65d43a76f6b87b6845a60f2d79d303</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00784-015-1453-0$$EPDF$$P50$$Gspringer$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00784-015-1453-0$$EHTML$$P50$$Gspringer$$Hfree_for_read</linktohtml><link.rule.ids>230,315,781,785,886,27929,27930,41493,42562,51324</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25843050$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ariani, Nina</creatorcontrib><creatorcontrib>Visser, Anita</creatorcontrib><creatorcontrib>Teulings, Margot R. I. M.</creatorcontrib><creatorcontrib>Dijk, Melissa</creatorcontrib><creatorcontrib>Rahardjo, Tri Budi W.</creatorcontrib><creatorcontrib>Vissink, Arjan</creatorcontrib><creatorcontrib>van der Mei, Henny C.</creatorcontrib><title>Efficacy of cleansing agents in killing microorganisms in mixed species biofilms present on silicone facial prostheses—an in vitro study</title><title>Clinical oral investigations</title><addtitle>Clin Oral Invest</addtitle><addtitle>Clin Oral Investig</addtitle><description>Objectives
The purpose of this study was to assess the efficacy of different cleansing agents in killing mixed species biofilms on silicone facial prostheses.
Materials and methods
Two bacterial and three yeast strains, isolated from silicone facial prostheses, were selected for the mixed species biofilms. A variety of agents used to clean facial prostheses were employed, viz., antibacterial soap, essential-oil-containing mouth rinse, ethanol 27 %, chlorhexidine mouth rinse, and buttermilk. Colony forming units (CFUs) and live/dead staining were analyzed to assess the efficacy of these cleansing agents against 24-h and 2-week biofilms and regrown biofilms on silicone samples.
Results
Chlorhexidine was the most effective cleansing agent. Chlorhexidine killed 8 log unit CFUs (>99.99 % killing) in a 24-h biofilm and 5 log unit CFUs (>99.99 % killing) in 2-week biofilms. Also, after regrowth and repeated treatment of the biofilm, chlorhexidine was the most effective cleansing agent showing no detectable CFUs. The essential-oil-containing mouth rinse (containing 26.9 % ethanol) showed a similar efficacy as ethanol (27 %) alone. Antibacterial soap and buttermilk were the least effective agents tested.
Conclusions
Chlorhexidine showed the highest reduction in CFUs in 24-h, 2-week, and regrown mixed species biofilm of microorganisms isolated from silicone facial prostheses.
Clinical relevance
Chlorhexidine mouth rinse (easy obtainable and relatively cheap) is very effective in killing bacteria and yeast present in biofilms on silicone facial prostheses. When applied on a regular basis, cleansing a facial prosthesis with chlorhexidine will presumably increase its lifetime and reduce skin irritations.</description><subject>Anti-Infective Agents, Local - pharmacology</subject><subject>Biofilms - drug effects</subject><subject>Buttermilk</subject><subject>Candida - growth & development</subject><subject>Chlorhexidine</subject><subject>Dentistry</subject><subject>Disinfectants - pharmacology</subject><subject>Ethanol</subject><subject>Face</subject><subject>Humans</subject><subject>Medicine</subject><subject>Mouthwashes</subject><subject>Oils, Volatile</subject><subject>Original</subject><subject>Original Article</subject><subject>Prostheses and Implants</subject><subject>Silicone Elastomers</subject><subject>Soaps</subject><subject>Staphylococcus - growth & development</subject><issn>1432-6981</issn><issn>1436-3771</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>C6C</sourceid><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqFkk1vFSEYhSfGxn7oD3BjSNy4GQvD18zGxDTVmjRxU9eEYWBKZeDKO9P07ly79hf6S2R6a1NNTDdAOA-H94VTVS8JfkswlsdQhpbVmPCaME5r_KQ6IIyKmkpJnt6um1p0LdmvDgGuMCZMSPqs2m94yyjm-KD6ceqcN9psUXLIBKsj-DgiPdo4A_IRffUhrDuTNzmlPOroYbpVJn9jBwQba7wF1PvkfCjKJlsoh1GKCHzwJkWLnDZehyIlmC-LDL--_9RxNbn2c04I5mXYPq_2nA5gX9zNR9WXD6cXJ2f1-eePn07en9eGSzrXrKUDlaVDzCzGXS-15W3T9tY6ggUxwjnBB0a1FE70rexFy7gW2DWD7AaK6VH1bue7WfrJDqYUm3VQm-wnnbcqaa_-VqK_VGO6VkxwIVlXDN7cGeT0bbEwq8mDsSHoaNMCirQN7cr7SvY4KqlghOGGF_T1P-hVWnIsL7FSnHeiY6JQZEeV3wDI1t3XTbBaQ6F2oVAlFGoNhVobfvWw4fsTf1JQgGYHQJHiaPODq__r-hvhucWH</recordid><startdate>20151201</startdate><enddate>20151201</enddate><creator>Ariani, Nina</creator><creator>Visser, Anita</creator><creator>Teulings, Margot R. 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M.</creator><creator>Dijk, Melissa</creator><creator>Rahardjo, Tri Budi W.</creator><creator>Vissink, Arjan</creator><creator>van der Mei, Henny C.</creator><general>Springer Berlin Heidelberg</general><general>Springer Nature B.V</general><scope>C6C</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>7X8</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>5PM</scope></search><sort><creationdate>20151201</creationdate><title>Efficacy of cleansing agents in killing microorganisms in mixed species biofilms present on silicone facial prostheses—an in vitro study</title><author>Ariani, Nina ; Visser, Anita ; Teulings, Margot R. I. M. ; Dijk, Melissa ; Rahardjo, Tri Budi W. ; Vissink, Arjan ; van der Mei, Henny C.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c573t-483d3737704e009b7ae5828beef1061c6ff65d43a76f6b87b6845a60f2d79d303</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Anti-Infective Agents, Local - pharmacology</topic><topic>Biofilms - drug effects</topic><topic>Buttermilk</topic><topic>Candida - growth & development</topic><topic>Chlorhexidine</topic><topic>Dentistry</topic><topic>Disinfectants - pharmacology</topic><topic>Ethanol</topic><topic>Face</topic><topic>Humans</topic><topic>Medicine</topic><topic>Mouthwashes</topic><topic>Oils, Volatile</topic><topic>Original</topic><topic>Original Article</topic><topic>Prostheses and Implants</topic><topic>Silicone Elastomers</topic><topic>Soaps</topic><topic>Staphylococcus - growth & development</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ariani, Nina</creatorcontrib><creatorcontrib>Visser, Anita</creatorcontrib><creatorcontrib>Teulings, Margot R. 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M.</creatorcontrib><creatorcontrib>Dijk, Melissa</creatorcontrib><creatorcontrib>Rahardjo, Tri Budi W.</creatorcontrib><creatorcontrib>Vissink, Arjan</creatorcontrib><creatorcontrib>van der Mei, Henny C.</creatorcontrib><collection>Springer Nature OA Free Journals</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection (ProQuest)</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biological Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>MEDLINE - Academic</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Clinical oral investigations</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ariani, Nina</au><au>Visser, Anita</au><au>Teulings, Margot R. I. M.</au><au>Dijk, Melissa</au><au>Rahardjo, Tri Budi W.</au><au>Vissink, Arjan</au><au>van der Mei, Henny C.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Efficacy of cleansing agents in killing microorganisms in mixed species biofilms present on silicone facial prostheses—an in vitro study</atitle><jtitle>Clinical oral investigations</jtitle><stitle>Clin Oral Invest</stitle><addtitle>Clin Oral Investig</addtitle><date>2015-12-01</date><risdate>2015</risdate><volume>19</volume><issue>9</issue><spage>2285</spage><epage>2293</epage><pages>2285-2293</pages><issn>1432-6981</issn><eissn>1436-3771</eissn><abstract>Objectives
The purpose of this study was to assess the efficacy of different cleansing agents in killing mixed species biofilms on silicone facial prostheses.
Materials and methods
Two bacterial and three yeast strains, isolated from silicone facial prostheses, were selected for the mixed species biofilms. A variety of agents used to clean facial prostheses were employed, viz., antibacterial soap, essential-oil-containing mouth rinse, ethanol 27 %, chlorhexidine mouth rinse, and buttermilk. Colony forming units (CFUs) and live/dead staining were analyzed to assess the efficacy of these cleansing agents against 24-h and 2-week biofilms and regrown biofilms on silicone samples.
Results
Chlorhexidine was the most effective cleansing agent. Chlorhexidine killed 8 log unit CFUs (>99.99 % killing) in a 24-h biofilm and 5 log unit CFUs (>99.99 % killing) in 2-week biofilms. Also, after regrowth and repeated treatment of the biofilm, chlorhexidine was the most effective cleansing agent showing no detectable CFUs. The essential-oil-containing mouth rinse (containing 26.9 % ethanol) showed a similar efficacy as ethanol (27 %) alone. Antibacterial soap and buttermilk were the least effective agents tested.
Conclusions
Chlorhexidine showed the highest reduction in CFUs in 24-h, 2-week, and regrown mixed species biofilm of microorganisms isolated from silicone facial prostheses.
Clinical relevance
Chlorhexidine mouth rinse (easy obtainable and relatively cheap) is very effective in killing bacteria and yeast present in biofilms on silicone facial prostheses. When applied on a regular basis, cleansing a facial prosthesis with chlorhexidine will presumably increase its lifetime and reduce skin irritations.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>25843050</pmid><doi>10.1007/s00784-015-1453-0</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Anti-Infective Agents, Local - pharmacology Biofilms - drug effects Buttermilk Candida - growth & development Chlorhexidine Dentistry Disinfectants - pharmacology Ethanol Face Humans Medicine Mouthwashes Oils, Volatile Original Original Article Prostheses and Implants Silicone Elastomers Soaps Staphylococcus - growth & development |
| title | Efficacy of cleansing agents in killing microorganisms in mixed species biofilms present on silicone facial prostheses—an in vitro study |
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