Visualizing viral protein structures in cells using genetic probes for correlated light and electron microscopy

•DAB is a small molecule that assembles into osmiophilic polymer upon oxidation.•Localized formation of DAB polymer enables identification of proteins inside cells in EM.•DAB can be oxidized by either photo-oxidation or enzymatic catalysis.•Immunostaining or genetically encoded tags can generate loc...

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Veröffentlicht in:	Methods (San Diego, Calif.) Calif.), 2015-11, Vol.90, p.39-48
Hauptverfasser:	Ou, Horng D., Deerinck, Thomas J., Bushong, Eric, Ellisman, Mark H., O’Shea, Clodagh C.
Format:	Artikel
Sprache:	eng
Schlagworte:	Adenoviral proteins Adenoviridae Cell Line E4-ORF3 polymer Electron microscopy Host-Pathogen Interactions Humans Microscopy, Electron - methods MiniSOG Models, Chemical Oxidation-Reduction Photo-oxidation Protein Structure, Tertiary Recombinant Fusion Proteins - chemistry Serial block face scanning electron microscopy Viral Proteins - chemistry
Online-Zugang:	Volltext
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