Simplified Protocol for Carba NP Test for Enhanced Detection of Carbapenemase Producers Directly from Bacterial Cultures

We compared carbapenemase detection among 266 Gram-negative bacilli (161 carbapenemase producers) using the Carba NP tests issued by the CLSI (CNPt-CLSI) and a novel protocol (CNPt-direct) designed for carbapenemase detection direct from bacterial cultures (instead of bacterial extracts required by...

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Veröffentlicht in:	Journal of clinical microbiology 2015-12, Vol.53 (12), p.3908-3911
Hauptverfasser:	Pasteran, Fernando, Tijet, Nathalie, Melano, Roberto G, Corso, Alejandra
Format:	Artikel
Sprache:	eng
Schlagworte:	Bacteria Bacterial Proteins - analysis Bacteriological Techniques - methods Bacteriology beta-Lactamases - analysis Gram-Negative Bacteria - enzymology Gram-Negative Bacteria - isolation & purification Humans Sensitivity and Specificity
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container_title	Journal of clinical microbiology
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creator	Pasteran, Fernando Tijet, Nathalie Melano, Roberto G Corso, Alejandra
description	We compared carbapenemase detection among 266 Gram-negative bacilli (161 carbapenemase producers) using the Carba NP tests issued by the CLSI (CNPt-CLSI) and a novel protocol (CNPt-direct) designed for carbapenemase detection direct from bacterial cultures (instead of bacterial extracts required by the CLSI tests). The specificities were comparable (100%), but the CNPt-direct was more sensitive (98% versus 84%). The CNPt-direct was easier to perform due to the direct use of colonies and offered a more robust detection of carbapenemase producers.
doi_str_mv	10.1128/JCM.02032-15
format	Article
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source	American Society for Microbiology; MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central
subjects	Bacteria Bacterial Proteins - analysis Bacteriological Techniques - methods Bacteriology beta-Lactamases - analysis Gram-Negative Bacteria - enzymology Gram-Negative Bacteria - isolation & purification Humans Sensitivity and Specificity
title	Simplified Protocol for Carba NP Test for Enhanced Detection of Carbapenemase Producers Directly from Bacterial Cultures
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