DW-F5: A novel formulation against malignant melanoma from Wrightia tinctoria
Wrightia tinctoria is a constituent of several ayurvedic preparations against skin disorders including psoriasis and herpes, though not yet has been explored for anticancer potential. Herein, for the first time, we report the significant anticancer properties of a semi-purified fraction, DW-F5, from...
Gespeichert in:
| Veröffentlicht in: | Scientific reports 2015-06, Vol.5 (1), p.11107, Article 11107 |
|---|---|
| Hauptverfasser: | , , , , , , , , , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | |
|---|---|
| container_issue | 1 |
| container_start_page | 11107 |
| container_title | Scientific reports |
| container_volume | 5 |
| creator | Antony, Jayesh Saikia, Minakshi V, Vinod Nath, Lekshmi. R. Katiki, Mohana Rao Murty, M.S.R. Paul, Anju A, Shabna Chandran, Harsha Joseph, Sophia Margaret S, Nishanth Kumar Panakkal, Elizabeth Jayex V, Sriramya I. V, Sridivya I. Ran, Sophia S, Sankar Rajan, Easwary Anto, Ruby John |
| description | Wrightia tinctoria
is a constituent of several ayurvedic preparations against skin disorders including psoriasis and herpes, though not yet has been explored for anticancer potential. Herein, for the first time, we report the significant anticancer properties of a semi-purified fraction, DW-F5, from the dichloromethane extract of
W. tinctoria
leaves against malignant melanoma. DW-F5 exhibited anti-melanoma activities, preventing metastasis and angiogenesis in NOD-SCID mice, while being non-toxic
in vivo
. The major pathways in melanoma signaling mediated through BRAF, WNT/β-catenin and Akt-NF-κB converging in MITF-M, the master regulator of melanomagenesis, were inhibited by DW-F5, leading to complete abolition of MITF-M. Purification of DW-F5 led to the isolation of two cytotoxic components, one being tryptanthrin and the other being an unidentified aliphatic fraction. The overall study predicts
Wrightia tinctoria
as a candidate plant to be further explored for anticancer properties and DW-F5 as a forthcoming drug formulation to be evaluated as a chemotherapeutic agent against malignant melanoma. |
| doi_str_mv | 10.1038/srep11107 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_4650611</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1899560075</sourcerecordid><originalsourceid>FETCH-LOGICAL-c438t-297641b7df7bdc1ffbe002a0d53a75aef32b5c9d0371dec3d3169c96ca1e2dd53</originalsourceid><addsrcrecordid>eNplkE9LAzEQxYMoVmoPfgFZ8KSwmmT_exBKtSpUvCg9htlssk3ZTWo2W_Dbm9JaKs5lBubHezMPoQuCbwmO8rvOihUhBGdH6IziOAlpROnxwTxAo65bYl8JLWJSnKIBTXFKcorP0NvjPJwm98E40GYtmkAa2_YNOGV0ADUo3bmghUbVGrSfRAPatBBIa9pgblW9cAoCpzR3xio4RycSmk6Mdn2IPqdPH5OXcPb-_DoZz0IeR7kLaZGlMSmzSmZlxYmUpcCYAq6SCLIEhIxomfCiwlFGKsGjKiJpwYuUAxG08tQQPWx1V33ZiooL7Sw0bGVVC_abGVDs70arBavNmsVp4j8nXuBqJ2DNVy86x5amt9rfzEheFEmKcbaxud5S3JrO5yz3DgSzTfhsH75nLw9P2pO_UXvgZgt0fqVrYQ8s_6n9AI23jwE</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1899560075</pqid></control><display><type>article</type><title>DW-F5: A novel formulation against malignant melanoma from Wrightia tinctoria</title><source>MEDLINE</source><source>DOAJ Directory of Open Access Journals</source><source>Springer Nature OA Free Journals</source><source>Nature Free</source><source>EZB-FREE-00999 freely available EZB journals</source><source>PubMed Central</source><source>Free Full-Text Journals in Chemistry</source><creator>Antony, Jayesh ; Saikia, Minakshi ; V, Vinod ; Nath, Lekshmi. R. ; Katiki, Mohana Rao ; Murty, M.S.R. ; Paul, Anju ; A, Shabna ; Chandran, Harsha ; Joseph, Sophia Margaret ; S, Nishanth Kumar ; Panakkal, Elizabeth Jayex ; V, Sriramya I. ; V, Sridivya I. ; Ran, Sophia ; S, Sankar ; Rajan, Easwary ; Anto, Ruby John</creator><creatorcontrib>Antony, Jayesh ; Saikia, Minakshi ; V, Vinod ; Nath, Lekshmi. R. ; Katiki, Mohana Rao ; Murty, M.S.R. ; Paul, Anju ; A, Shabna ; Chandran, Harsha ; Joseph, Sophia Margaret ; S, Nishanth Kumar ; Panakkal, Elizabeth Jayex ; V, Sriramya I. ; V, Sridivya I. ; Ran, Sophia ; S, Sankar ; Rajan, Easwary ; Anto, Ruby John</creatorcontrib><description>Wrightia tinctoria
is a constituent of several ayurvedic preparations against skin disorders including psoriasis and herpes, though not yet has been explored for anticancer potential. Herein, for the first time, we report the significant anticancer properties of a semi-purified fraction, DW-F5, from the dichloromethane extract of
W. tinctoria
leaves against malignant melanoma. DW-F5 exhibited anti-melanoma activities, preventing metastasis and angiogenesis in NOD-SCID mice, while being non-toxic
in vivo
. The major pathways in melanoma signaling mediated through BRAF, WNT/β-catenin and Akt-NF-κB converging in MITF-M, the master regulator of melanomagenesis, were inhibited by DW-F5, leading to complete abolition of MITF-M. Purification of DW-F5 led to the isolation of two cytotoxic components, one being tryptanthrin and the other being an unidentified aliphatic fraction. The overall study predicts
Wrightia tinctoria
as a candidate plant to be further explored for anticancer properties and DW-F5 as a forthcoming drug formulation to be evaluated as a chemotherapeutic agent against malignant melanoma.</description><identifier>ISSN: 2045-2322</identifier><identifier>EISSN: 2045-2322</identifier><identifier>DOI: 10.1038/srep11107</identifier><identifier>PMID: 26061820</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>13 ; 13/1 ; 13/106 ; 13/109 ; 13/2 ; 13/31 ; 13/51 ; 13/95 ; 14 ; 14/34 ; 59 ; 631/67/1813/1634 ; 64 ; 64/60 ; 692/699/67/1813 ; 82 ; 82/1 ; 82/16 ; 82/29 ; 82/51 ; 96 ; 96/1 ; 96/106 ; 96/109 ; 96/2 ; 96/63 ; AKT protein ; Angiogenesis ; Animals ; Antineoplastic Agents, Phytogenic - chemistry ; Antineoplastic Agents, Phytogenic - isolation & purification ; Antineoplastic Agents, Phytogenic - pharmacology ; Apocynaceae - chemistry ; Apoptosis - drug effects ; Cell Line, Tumor ; Cell Survival - drug effects ; Cell Transformation, Neoplastic - drug effects ; Cytotoxicity ; Dichloromethane ; Disease Models, Animal ; Humanities and Social Sciences ; Humans ; Melanoma ; Melanoma - drug therapy ; Melanoma - metabolism ; Melanoma - pathology ; Metastases ; Mice, Inbred NOD ; Mice, SCID ; Microphthalmia-associated transcription factor ; multidisciplinary ; Neoplasm Metastasis ; Neovascularization, Pathologic - drug therapy ; Plant Extracts - pharmacology ; Plant Leaves - chemistry ; Psoriasis ; Purification ; Quinazolines - chemistry ; Quinazolines - pharmacology ; Science ; Science (multidisciplinary) ; Signal Transduction - drug effects ; Skin cancer ; Skin diseases ; Wnt protein ; Xenograft Model Antitumor Assays ; β-Catenin</subject><ispartof>Scientific reports, 2015-06, Vol.5 (1), p.11107, Article 11107</ispartof><rights>The Author(s) 2015</rights><rights>Copyright Nature Publishing Group Jun 2015</rights><rights>Copyright © 2015, Macmillan Publishers Limited 2015 Macmillan Publishers Limited</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c438t-297641b7df7bdc1ffbe002a0d53a75aef32b5c9d0371dec3d3169c96ca1e2dd53</citedby><cites>FETCH-LOGICAL-c438t-297641b7df7bdc1ffbe002a0d53a75aef32b5c9d0371dec3d3169c96ca1e2dd53</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4650611/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4650611/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,27924,27925,41120,42189,51576,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26061820$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Antony, Jayesh</creatorcontrib><creatorcontrib>Saikia, Minakshi</creatorcontrib><creatorcontrib>V, Vinod</creatorcontrib><creatorcontrib>Nath, Lekshmi. R.</creatorcontrib><creatorcontrib>Katiki, Mohana Rao</creatorcontrib><creatorcontrib>Murty, M.S.R.</creatorcontrib><creatorcontrib>Paul, Anju</creatorcontrib><creatorcontrib>A, Shabna</creatorcontrib><creatorcontrib>Chandran, Harsha</creatorcontrib><creatorcontrib>Joseph, Sophia Margaret</creatorcontrib><creatorcontrib>S, Nishanth Kumar</creatorcontrib><creatorcontrib>Panakkal, Elizabeth Jayex</creatorcontrib><creatorcontrib>V, Sriramya I.</creatorcontrib><creatorcontrib>V, Sridivya I.</creatorcontrib><creatorcontrib>Ran, Sophia</creatorcontrib><creatorcontrib>S, Sankar</creatorcontrib><creatorcontrib>Rajan, Easwary</creatorcontrib><creatorcontrib>Anto, Ruby John</creatorcontrib><title>DW-F5: A novel formulation against malignant melanoma from Wrightia tinctoria</title><title>Scientific reports</title><addtitle>Sci Rep</addtitle><addtitle>Sci Rep</addtitle><description>Wrightia tinctoria
is a constituent of several ayurvedic preparations against skin disorders including psoriasis and herpes, though not yet has been explored for anticancer potential. Herein, for the first time, we report the significant anticancer properties of a semi-purified fraction, DW-F5, from the dichloromethane extract of
W. tinctoria
leaves against malignant melanoma. DW-F5 exhibited anti-melanoma activities, preventing metastasis and angiogenesis in NOD-SCID mice, while being non-toxic
in vivo
. The major pathways in melanoma signaling mediated through BRAF, WNT/β-catenin and Akt-NF-κB converging in MITF-M, the master regulator of melanomagenesis, were inhibited by DW-F5, leading to complete abolition of MITF-M. Purification of DW-F5 led to the isolation of two cytotoxic components, one being tryptanthrin and the other being an unidentified aliphatic fraction. The overall study predicts
Wrightia tinctoria
as a candidate plant to be further explored for anticancer properties and DW-F5 as a forthcoming drug formulation to be evaluated as a chemotherapeutic agent against malignant melanoma.</description><subject>13</subject><subject>13/1</subject><subject>13/106</subject><subject>13/109</subject><subject>13/2</subject><subject>13/31</subject><subject>13/51</subject><subject>13/95</subject><subject>14</subject><subject>14/34</subject><subject>59</subject><subject>631/67/1813/1634</subject><subject>64</subject><subject>64/60</subject><subject>692/699/67/1813</subject><subject>82</subject><subject>82/1</subject><subject>82/16</subject><subject>82/29</subject><subject>82/51</subject><subject>96</subject><subject>96/1</subject><subject>96/106</subject><subject>96/109</subject><subject>96/2</subject><subject>96/63</subject><subject>AKT protein</subject><subject>Angiogenesis</subject><subject>Animals</subject><subject>Antineoplastic Agents, Phytogenic - chemistry</subject><subject>Antineoplastic Agents, Phytogenic - isolation & purification</subject><subject>Antineoplastic Agents, Phytogenic - pharmacology</subject><subject>Apocynaceae - chemistry</subject><subject>Apoptosis - drug effects</subject><subject>Cell Line, Tumor</subject><subject>Cell Survival - drug effects</subject><subject>Cell Transformation, Neoplastic - drug effects</subject><subject>Cytotoxicity</subject><subject>Dichloromethane</subject><subject>Disease Models, Animal</subject><subject>Humanities and Social Sciences</subject><subject>Humans</subject><subject>Melanoma</subject><subject>Melanoma - drug therapy</subject><subject>Melanoma - metabolism</subject><subject>Melanoma - pathology</subject><subject>Metastases</subject><subject>Mice, Inbred NOD</subject><subject>Mice, SCID</subject><subject>Microphthalmia-associated transcription factor</subject><subject>multidisciplinary</subject><subject>Neoplasm Metastasis</subject><subject>Neovascularization, Pathologic - drug therapy</subject><subject>Plant Extracts - pharmacology</subject><subject>Plant Leaves - chemistry</subject><subject>Psoriasis</subject><subject>Purification</subject><subject>Quinazolines - chemistry</subject><subject>Quinazolines - pharmacology</subject><subject>Science</subject><subject>Science (multidisciplinary)</subject><subject>Signal Transduction - drug effects</subject><subject>Skin cancer</subject><subject>Skin diseases</subject><subject>Wnt protein</subject><subject>Xenograft Model Antitumor Assays</subject><subject>β-Catenin</subject><issn>2045-2322</issn><issn>2045-2322</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>C6C</sourceid><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNplkE9LAzEQxYMoVmoPfgFZ8KSwmmT_exBKtSpUvCg9htlssk3ZTWo2W_Dbm9JaKs5lBubHezMPoQuCbwmO8rvOihUhBGdH6IziOAlpROnxwTxAo65bYl8JLWJSnKIBTXFKcorP0NvjPJwm98E40GYtmkAa2_YNOGV0ADUo3bmghUbVGrSfRAPatBBIa9pgblW9cAoCpzR3xio4RycSmk6Mdn2IPqdPH5OXcPb-_DoZz0IeR7kLaZGlMSmzSmZlxYmUpcCYAq6SCLIEhIxomfCiwlFGKsGjKiJpwYuUAxG08tQQPWx1V33ZiooL7Sw0bGVVC_abGVDs70arBavNmsVp4j8nXuBqJ2DNVy86x5amt9rfzEheFEmKcbaxud5S3JrO5yz3DgSzTfhsH75nLw9P2pO_UXvgZgt0fqVrYQ8s_6n9AI23jwE</recordid><startdate>20150610</startdate><enddate>20150610</enddate><creator>Antony, Jayesh</creator><creator>Saikia, Minakshi</creator><creator>V, Vinod</creator><creator>Nath, Lekshmi. R.</creator><creator>Katiki, Mohana Rao</creator><creator>Murty, M.S.R.</creator><creator>Paul, Anju</creator><creator>A, Shabna</creator><creator>Chandran, Harsha</creator><creator>Joseph, Sophia Margaret</creator><creator>S, Nishanth Kumar</creator><creator>Panakkal, Elizabeth Jayex</creator><creator>V, Sriramya I.</creator><creator>V, Sridivya I.</creator><creator>Ran, Sophia</creator><creator>S, Sankar</creator><creator>Rajan, Easwary</creator><creator>Anto, Ruby John</creator><general>Nature Publishing Group UK</general><general>Nature Publishing Group</general><scope>C6C</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>5PM</scope></search><sort><creationdate>20150610</creationdate><title>DW-F5: A novel formulation against malignant melanoma from Wrightia tinctoria</title><author>Antony, Jayesh ; Saikia, Minakshi ; V, Vinod ; Nath, Lekshmi. R. ; Katiki, Mohana Rao ; Murty, M.S.R. ; Paul, Anju ; A, Shabna ; Chandran, Harsha ; Joseph, Sophia Margaret ; S, Nishanth Kumar ; Panakkal, Elizabeth Jayex ; V, Sriramya I. ; V, Sridivya I. ; Ran, Sophia ; S, Sankar ; Rajan, Easwary ; Anto, Ruby John</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c438t-297641b7df7bdc1ffbe002a0d53a75aef32b5c9d0371dec3d3169c96ca1e2dd53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>13</topic><topic>13/1</topic><topic>13/106</topic><topic>13/109</topic><topic>13/2</topic><topic>13/31</topic><topic>13/51</topic><topic>13/95</topic><topic>14</topic><topic>14/34</topic><topic>59</topic><topic>631/67/1813/1634</topic><topic>64</topic><topic>64/60</topic><topic>692/699/67/1813</topic><topic>82</topic><topic>82/1</topic><topic>82/16</topic><topic>82/29</topic><topic>82/51</topic><topic>96</topic><topic>96/1</topic><topic>96/106</topic><topic>96/109</topic><topic>96/2</topic><topic>96/63</topic><topic>AKT protein</topic><topic>Angiogenesis</topic><topic>Animals</topic><topic>Antineoplastic Agents, Phytogenic - chemistry</topic><topic>Antineoplastic Agents, Phytogenic - isolation & purification</topic><topic>Antineoplastic Agents, Phytogenic - pharmacology</topic><topic>Apocynaceae - chemistry</topic><topic>Apoptosis - drug effects</topic><topic>Cell Line, Tumor</topic><topic>Cell Survival - drug effects</topic><topic>Cell Transformation, Neoplastic - drug effects</topic><topic>Cytotoxicity</topic><topic>Dichloromethane</topic><topic>Disease Models, Animal</topic><topic>Humanities and Social Sciences</topic><topic>Humans</topic><topic>Melanoma</topic><topic>Melanoma - drug therapy</topic><topic>Melanoma - metabolism</topic><topic>Melanoma - pathology</topic><topic>Metastases</topic><topic>Mice, Inbred NOD</topic><topic>Mice, SCID</topic><topic>Microphthalmia-associated transcription factor</topic><topic>multidisciplinary</topic><topic>Neoplasm Metastasis</topic><topic>Neovascularization, Pathologic - drug therapy</topic><topic>Plant Extracts - pharmacology</topic><topic>Plant Leaves - chemistry</topic><topic>Psoriasis</topic><topic>Purification</topic><topic>Quinazolines - chemistry</topic><topic>Quinazolines - pharmacology</topic><topic>Science</topic><topic>Science (multidisciplinary)</topic><topic>Signal Transduction - drug effects</topic><topic>Skin cancer</topic><topic>Skin diseases</topic><topic>Wnt protein</topic><topic>Xenograft Model Antitumor Assays</topic><topic>β-Catenin</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Antony, Jayesh</creatorcontrib><creatorcontrib>Saikia, Minakshi</creatorcontrib><creatorcontrib>V, Vinod</creatorcontrib><creatorcontrib>Nath, Lekshmi. R.</creatorcontrib><creatorcontrib>Katiki, Mohana Rao</creatorcontrib><creatorcontrib>Murty, M.S.R.</creatorcontrib><creatorcontrib>Paul, Anju</creatorcontrib><creatorcontrib>A, Shabna</creatorcontrib><creatorcontrib>Chandran, Harsha</creatorcontrib><creatorcontrib>Joseph, Sophia Margaret</creatorcontrib><creatorcontrib>S, Nishanth Kumar</creatorcontrib><creatorcontrib>Panakkal, Elizabeth Jayex</creatorcontrib><creatorcontrib>V, Sriramya I.</creatorcontrib><creatorcontrib>V, Sridivya I.</creatorcontrib><creatorcontrib>Ran, Sophia</creatorcontrib><creatorcontrib>S, Sankar</creatorcontrib><creatorcontrib>Rajan, Easwary</creatorcontrib><creatorcontrib>Anto, Ruby John</creatorcontrib><collection>Springer Nature OA Free Journals</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Biological Science Database</collection><collection>Access via ProQuest (Open Access)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Scientific reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Antony, Jayesh</au><au>Saikia, Minakshi</au><au>V, Vinod</au><au>Nath, Lekshmi. R.</au><au>Katiki, Mohana Rao</au><au>Murty, M.S.R.</au><au>Paul, Anju</au><au>A, Shabna</au><au>Chandran, Harsha</au><au>Joseph, Sophia Margaret</au><au>S, Nishanth Kumar</au><au>Panakkal, Elizabeth Jayex</au><au>V, Sriramya I.</au><au>V, Sridivya I.</au><au>Ran, Sophia</au><au>S, Sankar</au><au>Rajan, Easwary</au><au>Anto, Ruby John</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>DW-F5: A novel formulation against malignant melanoma from Wrightia tinctoria</atitle><jtitle>Scientific reports</jtitle><stitle>Sci Rep</stitle><addtitle>Sci Rep</addtitle><date>2015-06-10</date><risdate>2015</risdate><volume>5</volume><issue>1</issue><spage>11107</spage><pages>11107-</pages><artnum>11107</artnum><issn>2045-2322</issn><eissn>2045-2322</eissn><abstract>Wrightia tinctoria
is a constituent of several ayurvedic preparations against skin disorders including psoriasis and herpes, though not yet has been explored for anticancer potential. Herein, for the first time, we report the significant anticancer properties of a semi-purified fraction, DW-F5, from the dichloromethane extract of
W. tinctoria
leaves against malignant melanoma. DW-F5 exhibited anti-melanoma activities, preventing metastasis and angiogenesis in NOD-SCID mice, while being non-toxic
in vivo
. The major pathways in melanoma signaling mediated through BRAF, WNT/β-catenin and Akt-NF-κB converging in MITF-M, the master regulator of melanomagenesis, were inhibited by DW-F5, leading to complete abolition of MITF-M. Purification of DW-F5 led to the isolation of two cytotoxic components, one being tryptanthrin and the other being an unidentified aliphatic fraction. The overall study predicts
Wrightia tinctoria
as a candidate plant to be further explored for anticancer properties and DW-F5 as a forthcoming drug formulation to be evaluated as a chemotherapeutic agent against malignant melanoma.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>26061820</pmid><doi>10.1038/srep11107</doi><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 2045-2322 |
| ispartof | Scientific reports, 2015-06, Vol.5 (1), p.11107, Article 11107 |
| issn | 2045-2322 2045-2322 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_4650611 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; Springer Nature OA Free Journals; Nature Free; EZB-FREE-00999 freely available EZB journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | 13 13/1 13/106 13/109 13/2 13/31 13/51 13/95 14 14/34 59 631/67/1813/1634 64 64/60 692/699/67/1813 82 82/1 82/16 82/29 82/51 96 96/1 96/106 96/109 96/2 96/63 AKT protein Angiogenesis Animals Antineoplastic Agents, Phytogenic - chemistry Antineoplastic Agents, Phytogenic - isolation & purification Antineoplastic Agents, Phytogenic - pharmacology Apocynaceae - chemistry Apoptosis - drug effects Cell Line, Tumor Cell Survival - drug effects Cell Transformation, Neoplastic - drug effects Cytotoxicity Dichloromethane Disease Models, Animal Humanities and Social Sciences Humans Melanoma Melanoma - drug therapy Melanoma - metabolism Melanoma - pathology Metastases Mice, Inbred NOD Mice, SCID Microphthalmia-associated transcription factor multidisciplinary Neoplasm Metastasis Neovascularization, Pathologic - drug therapy Plant Extracts - pharmacology Plant Leaves - chemistry Psoriasis Purification Quinazolines - chemistry Quinazolines - pharmacology Science Science (multidisciplinary) Signal Transduction - drug effects Skin cancer Skin diseases Wnt protein Xenograft Model Antitumor Assays β-Catenin |
| title | DW-F5: A novel formulation against malignant melanoma from Wrightia tinctoria |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-22T04%3A02%3A19IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=DW-F5:%20A%20novel%20formulation%20against%20malignant%20melanoma%20from%20Wrightia%20tinctoria&rft.jtitle=Scientific%20reports&rft.au=Antony,%20Jayesh&rft.date=2015-06-10&rft.volume=5&rft.issue=1&rft.spage=11107&rft.pages=11107-&rft.artnum=11107&rft.issn=2045-2322&rft.eissn=2045-2322&rft_id=info:doi/10.1038/srep11107&rft_dat=%3Cproquest_pubme%3E1899560075%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1899560075&rft_id=info:pmid/26061820&rfr_iscdi=true |