Activation of Antibiotic Production in Bacillus spp. by Cumulative Drug Resistance Mutations
Bacillus subtilis strains produce a wide range of antibiotics, including ribosomal and nonribosomal peptide antibiotics, as well as bacilysocin and neotrehalosadiamine. Mutations in B. subtilis strain 168 that conferred resistance to drugs such as streptomycin and rifampin resulted in overproduction...
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| Veröffentlicht in: | Antimicrobial agents and chemotherapy 2015-12, Vol.59 (12), p.7799-7804 |
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| creator | Tojo, Shigeo Tanaka, Yukinori Ochi, Kozo |
| description | Bacillus subtilis strains produce a wide range of antibiotics, including ribosomal and nonribosomal peptide antibiotics, as well as bacilysocin and neotrehalosadiamine. Mutations in B. subtilis strain 168 that conferred resistance to drugs such as streptomycin and rifampin resulted in overproduction of the dipeptide antibiotic bacilysin. Cumulative drug resistance mutations, such as mutations in the mthA and rpsL genes, which confer low- and high-level resistance, respectively, to streptomycin, and mutations in rpoB, which confer resistance to rifampin, resulted in cells that overproduced bacilysin. Transcriptional analysis demonstrated that the enhanced transcription of biosynthesis genes was responsible for the overproduction of bacilysin. This approach was effective also in activating the cryptic genes of Bacillus amyloliquefaciens, leading to actual production of antibiotic(s). |
| doi_str_mv | 10.1128/aac.01932-15 |
| format | Article |
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Mutations in B. subtilis strain 168 that conferred resistance to drugs such as streptomycin and rifampin resulted in overproduction of the dipeptide antibiotic bacilysin. Cumulative drug resistance mutations, such as mutations in the mthA and rpsL genes, which confer low- and high-level resistance, respectively, to streptomycin, and mutations in rpoB, which confer resistance to rifampin, resulted in cells that overproduced bacilysin. Transcriptional analysis demonstrated that the enhanced transcription of biosynthesis genes was responsible for the overproduction of bacilysin. This approach was effective also in activating the cryptic genes of Bacillus amyloliquefaciens, leading to actual production of antibiotic(s).</description><identifier>ISSN: 0066-4804</identifier><identifier>EISSN: 1098-6596</identifier><identifier>DOI: 10.1128/aac.01932-15</identifier><identifier>PMID: 26369962</identifier><language>eng</language><publisher>United States: American Society for Microbiology</publisher><subject>Anti-Bacterial Agents ; Anti-Bacterial Agents - biosynthesis ; Anti-Bacterial Agents - chemistry ; Anti-Bacterial Agents - pharmacology ; Bacillus ; Bacillus - genetics ; Bacillus - growth & development ; Bacillus - metabolism ; Bacillus amyloliquefaciens ; Bacillus subtilis ; Culture Media ; Dipeptides - biosynthesis ; Dipeptides - pharmacology ; Drug Resistance, Bacterial ; Drug Resistance, Bacterial - genetics ; Glucosidases - genetics ; Mechanisms of Resistance ; Mutation ; Mutation - genetics ; Ribosomal Proteins - genetics ; Rifampin - pharmacology</subject><ispartof>Antimicrobial agents and chemotherapy, 2015-12, Vol.59 (12), p.7799-7804</ispartof><rights>Copyright © 2015, American Society for Microbiology. All Rights Reserved.</rights><rights>Copyright © 2015, American Society for Microbiology. All Rights Reserved. 2015 American Society for Microbiology</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a517t-2381a71b076feb3c4ae8a68cf2fc6f1a4fa186377575fa295a87fae611ad89c53</citedby><cites>FETCH-LOGICAL-a517t-2381a71b076feb3c4ae8a68cf2fc6f1a4fa186377575fa295a87fae611ad89c53</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4649196/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4649196/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26369962$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Tojo, Shigeo</creatorcontrib><creatorcontrib>Tanaka, Yukinori</creatorcontrib><creatorcontrib>Ochi, Kozo</creatorcontrib><title>Activation of Antibiotic Production in Bacillus spp. by Cumulative Drug Resistance Mutations</title><title>Antimicrobial agents and chemotherapy</title><addtitle>Antimicrob Agents Chemother</addtitle><addtitle>Antimicrob Agents Chemother</addtitle><description>Bacillus subtilis strains produce a wide range of antibiotics, including ribosomal and nonribosomal peptide antibiotics, as well as bacilysocin and neotrehalosadiamine. 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Tanaka, Yukinori ; Ochi, Kozo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a517t-2381a71b076feb3c4ae8a68cf2fc6f1a4fa186377575fa295a87fae611ad89c53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Anti-Bacterial Agents</topic><topic>Anti-Bacterial Agents - biosynthesis</topic><topic>Anti-Bacterial Agents - chemistry</topic><topic>Anti-Bacterial Agents - pharmacology</topic><topic>Bacillus</topic><topic>Bacillus - genetics</topic><topic>Bacillus - growth & development</topic><topic>Bacillus - metabolism</topic><topic>Bacillus amyloliquefaciens</topic><topic>Bacillus subtilis</topic><topic>Culture Media</topic><topic>Dipeptides - biosynthesis</topic><topic>Dipeptides - pharmacology</topic><topic>Drug Resistance, Bacterial</topic><topic>Drug Resistance, Bacterial - genetics</topic><topic>Glucosidases - genetics</topic><topic>Mechanisms of Resistance</topic><topic>Mutation</topic><topic>Mutation - genetics</topic><topic>Ribosomal Proteins - genetics</topic><topic>Rifampin - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Tojo, Shigeo</creatorcontrib><creatorcontrib>Tanaka, Yukinori</creatorcontrib><creatorcontrib>Ochi, Kozo</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Antimicrobial agents and chemotherapy</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tojo, Shigeo</au><au>Tanaka, Yukinori</au><au>Ochi, Kozo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Activation of Antibiotic Production in Bacillus spp. by Cumulative Drug Resistance Mutations</atitle><jtitle>Antimicrobial agents and chemotherapy</jtitle><stitle>Antimicrob Agents Chemother</stitle><addtitle>Antimicrob Agents Chemother</addtitle><date>2015-12-01</date><risdate>2015</risdate><volume>59</volume><issue>12</issue><spage>7799</spage><epage>7804</epage><pages>7799-7804</pages><issn>0066-4804</issn><eissn>1098-6596</eissn><abstract>Bacillus subtilis strains produce a wide range of antibiotics, including ribosomal and nonribosomal peptide antibiotics, as well as bacilysocin and neotrehalosadiamine. 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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central |
| subjects | Anti-Bacterial Agents Anti-Bacterial Agents - biosynthesis Anti-Bacterial Agents - chemistry Anti-Bacterial Agents - pharmacology Bacillus Bacillus - genetics Bacillus - growth & development Bacillus - metabolism Bacillus amyloliquefaciens Bacillus subtilis Culture Media Dipeptides - biosynthesis Dipeptides - pharmacology Drug Resistance, Bacterial Drug Resistance, Bacterial - genetics Glucosidases - genetics Mechanisms of Resistance Mutation Mutation - genetics Ribosomal Proteins - genetics Rifampin - pharmacology |
| title | Activation of Antibiotic Production in Bacillus spp. by Cumulative Drug Resistance Mutations |
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