Large scale systematic proteomic quantification from non-metastatic to metastatic colorectal cancer
A systematic proteomic quantification of formalin-fixed, paraffin-embedded (FFPE) colorectal cancer tissues from stage I to stage IIIC was performed in large scale. 1017 proteins were identified with 338 proteins in quantitative changes by label free method, while 341 proteins were quantified with s...
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| Veröffentlicht in: | Scientific reports 2015-07, Vol.5 (1), p.12120-12120, Article 12120 |
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| description | A systematic proteomic quantification of formalin-fixed, paraffin-embedded (FFPE) colorectal cancer tissues from stage I to stage IIIC was performed in large scale. 1017 proteins were identified with 338 proteins in quantitative changes by label free method, while 341 proteins were quantified with significant expression changes among 6294 proteins by iTRAQ method. We found that proteins related to migration expression increased and those for binding and adherent decreased during the colorectal cancer development according to the gene ontology (GO) annotation and ingenuity pathway analysis (IPA). The integrin alpha 5 (ITA5) in integrin family was focused, which was consistent with the metastasis related pathway. The expression level of ITA5 decreased in metastasis tissues and the result has been further verified by Western blotting. Another two cell migration related proteins vitronectin (VTN) and actin-related protein (ARP3) were also proved to be up-regulated by both mass spectrometry (MS) based quantification results and Western blotting. Up to now, our result shows one of the largest dataset in colorectal cancer proteomics research. Our strategy reveals a disease driven omics-pattern for the metastasis colorectal cancer. |
| doi_str_mv | 10.1038/srep12120 |
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We found that proteins related to migration expression increased and those for binding and adherent decreased during the colorectal cancer development according to the gene ontology (GO) annotation and ingenuity pathway analysis (IPA). The integrin alpha 5 (ITA5) in integrin family was focused, which was consistent with the metastasis related pathway. The expression level of ITA5 decreased in metastasis tissues and the result has been further verified by Western blotting. Another two cell migration related proteins vitronectin (VTN) and actin-related protein (ARP3) were also proved to be up-regulated by both mass spectrometry (MS) based quantification results and Western blotting. Up to now, our result shows one of the largest dataset in colorectal cancer proteomics research. Our strategy reveals a disease driven omics-pattern for the metastasis colorectal cancer.</description><identifier>ISSN: 2045-2322</identifier><identifier>EISSN: 2045-2322</identifier><identifier>DOI: 10.1038/srep12120</identifier><identifier>PMID: 26175278</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>38/39 ; 631/45/475/2290 ; 631/67/2327 ; 639/638/11/872 ; 82/16 ; 82/29 ; 82/58 ; 82/80 ; Actin ; Actin-Related Protein 3 - metabolism ; Biomarkers, Tumor - analysis ; Blotting, Western ; Cell adhesion & migration ; Cell migration ; Cell Movement ; Chromatography, High Pressure Liquid ; Colorectal cancer ; Colorectal carcinoma ; Colorectal Neoplasms - metabolism ; Colorectal Neoplasms - pathology ; Databases, Factual ; Electrophoresis, Polyacrylamide Gel ; Formaldehyde ; Humanities and Social Sciences ; Humans ; Integrin alphaV - metabolism ; Mass spectrometry ; Mass spectroscopy ; Metabolic Networks and Pathways ; Metastases ; Metastasis ; multidisciplinary ; Neoplasm Metastasis ; Paraffin ; Paraffin Embedding ; Proteins ; Proteome - analysis ; Proteomics ; Science ; Tandem Mass Spectrometry ; Vitronectin ; Vitronectin - metabolism ; Western blotting</subject><ispartof>Scientific reports, 2015-07, Vol.5 (1), p.12120-12120, Article 12120</ispartof><rights>The Author(s) 2015</rights><rights>Copyright Nature Publishing Group Jul 2015</rights><rights>Copyright © 2015, Macmillan Publishers Limited 2015 Macmillan Publishers Limited</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c438t-53dca5b90c8c9200f8c2f31eec6d8702abfcf4775ee57218ec118f66cd95909c3</citedby><cites>FETCH-LOGICAL-c438t-53dca5b90c8c9200f8c2f31eec6d8702abfcf4775ee57218ec118f66cd95909c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4648419/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4648419/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,27924,27925,41120,42189,51576,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26175278$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Yin, Xuefei</creatorcontrib><creatorcontrib>Zhang, Yang</creatorcontrib><creatorcontrib>Guo, Shaowen</creatorcontrib><creatorcontrib>Jin, Hong</creatorcontrib><creatorcontrib>Wang, Wenhai</creatorcontrib><creatorcontrib>Yang, Pengyuan</creatorcontrib><title>Large scale systematic proteomic quantification from non-metastatic to metastatic colorectal cancer</title><title>Scientific reports</title><addtitle>Sci Rep</addtitle><addtitle>Sci Rep</addtitle><description>A systematic proteomic quantification of formalin-fixed, paraffin-embedded (FFPE) colorectal cancer tissues from stage I to stage IIIC was performed in large scale. 1017 proteins were identified with 338 proteins in quantitative changes by label free method, while 341 proteins were quantified with significant expression changes among 6294 proteins by iTRAQ method. We found that proteins related to migration expression increased and those for binding and adherent decreased during the colorectal cancer development according to the gene ontology (GO) annotation and ingenuity pathway analysis (IPA). The integrin alpha 5 (ITA5) in integrin family was focused, which was consistent with the metastasis related pathway. The expression level of ITA5 decreased in metastasis tissues and the result has been further verified by Western blotting. Another two cell migration related proteins vitronectin (VTN) and actin-related protein (ARP3) were also proved to be up-regulated by both mass spectrometry (MS) based quantification results and Western blotting. Up to now, our result shows one of the largest dataset in colorectal cancer proteomics research. 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Scientific reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yin, Xuefei</au><au>Zhang, Yang</au><au>Guo, Shaowen</au><au>Jin, Hong</au><au>Wang, Wenhai</au><au>Yang, Pengyuan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Large scale systematic proteomic quantification from non-metastatic to metastatic colorectal cancer</atitle><jtitle>Scientific reports</jtitle><stitle>Sci Rep</stitle><addtitle>Sci Rep</addtitle><date>2015-07-15</date><risdate>2015</risdate><volume>5</volume><issue>1</issue><spage>12120</spage><epage>12120</epage><pages>12120-12120</pages><artnum>12120</artnum><issn>2045-2322</issn><eissn>2045-2322</eissn><abstract>A systematic proteomic quantification of formalin-fixed, paraffin-embedded (FFPE) colorectal cancer tissues from stage I to stage IIIC was performed in large scale. 1017 proteins were identified with 338 proteins in quantitative changes by label free method, while 341 proteins were quantified with significant expression changes among 6294 proteins by iTRAQ method. We found that proteins related to migration expression increased and those for binding and adherent decreased during the colorectal cancer development according to the gene ontology (GO) annotation and ingenuity pathway analysis (IPA). The integrin alpha 5 (ITA5) in integrin family was focused, which was consistent with the metastasis related pathway. The expression level of ITA5 decreased in metastasis tissues and the result has been further verified by Western blotting. Another two cell migration related proteins vitronectin (VTN) and actin-related protein (ARP3) were also proved to be up-regulated by both mass spectrometry (MS) based quantification results and Western blotting. Up to now, our result shows one of the largest dataset in colorectal cancer proteomics research. Our strategy reveals a disease driven omics-pattern for the metastasis colorectal cancer.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>26175278</pmid><doi>10.1038/srep12120</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | 38/39 631/45/475/2290 631/67/2327 639/638/11/872 82/16 82/29 82/58 82/80 Actin Actin-Related Protein 3 - metabolism Biomarkers, Tumor - analysis Blotting, Western Cell adhesion & migration Cell migration Cell Movement Chromatography, High Pressure Liquid Colorectal cancer Colorectal carcinoma Colorectal Neoplasms - metabolism Colorectal Neoplasms - pathology Databases, Factual Electrophoresis, Polyacrylamide Gel Formaldehyde Humanities and Social Sciences Humans Integrin alphaV - metabolism Mass spectrometry Mass spectroscopy Metabolic Networks and Pathways Metastases Metastasis multidisciplinary Neoplasm Metastasis Paraffin Paraffin Embedding Proteins Proteome - analysis Proteomics Science Tandem Mass Spectrometry Vitronectin Vitronectin - metabolism Western blotting |
| title | Large scale systematic proteomic quantification from non-metastatic to metastatic colorectal cancer |
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