I-SceI-mediated double-strand DNA breaks stimulate efficient gene targeting in the industrial fungus Trichoderma reesei

Targeted integration of expression cassettes for enzyme production in industrial microorganisms is desirable especially when enzyme variants are screened for improved enzymatic properties. However, currently used methods for targeted integration are inefficient and result in low transformation frequ...

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Veröffentlicht in:	Applied microbiology and biotechnology 2015-12, Vol.99 (23), p.10083-10095
Hauptverfasser:	Ouedraogo, Jean Paul, Arentshorst, Mark, Nikolaev, Igor, Barends, Sharief, Ram, Arthur F. J
Format:	Artikel
Sprache:	eng
Schlagworte:	Analysis Applied Genetics and Molecular Biotechnology Biomedical and Life Sciences Biotechnology Deoxyribonucleases, Type II Site-Specific - genetics Deoxyribonucleases, Type II Site-Specific - metabolism Deoxyribonucleic acid DNA DNA Breaks, Double-Stranded DNA damage DNA libraries Efficiency Enzymes Fungi Gene Expression gene targeting Gene Targeting - methods Genetic recombination genome Genomes glucan 1,4-alpha-glucosidase Glucan 1,4-alpha-Glucosidase - genetics Glucan 1,4-alpha-Glucosidase - metabolism glucoamylase homologous recombination Industrial Microbiology - methods integration Lactose Life Sciences loci Metabolic Engineering - methods Microbial Genetics and Genomics Microbiology Microorganisms molecular cloning Polyethylene glycol Proteins Recombinant Proteins - genetics Recombinant Proteins - metabolism Saccharomyces cerevisiae Saccharomyces cerevisiae Proteins - genetics Saccharomyces cerevisiae Proteins - metabolism screening Studies transformation Transformation, Genetic Trichoderma - genetics Trichoderma reesei
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