Dynamic subnuclear relocalisation of WRKY40 in response to Abscisic acid in Arabidopsis thaliana
WRKY18, WRKY40 and WRKY60 are members of the WRKY transcription factor family and function as transcriptional regulators in ABA signal transduction in Arabidopsis thaliana . Here we show that WRKY18 and WRKY40, but not WRKY60, co-localise with PIF3, PIF4 and PHYB to Phytochrome B-containing nuclear...
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| Veröffentlicht in: | Scientific reports 2015-08, Vol.5 (1), p.13369-13369, Article 13369 |
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| description | WRKY18, WRKY40 and WRKY60 are members of the WRKY transcription factor family and function as transcriptional regulators in ABA signal transduction in
Arabidopsis thaliana
. Here we show that WRKY18 and WRKY40, but not WRKY60, co-localise with PIF3, PIF4 and PHYB to Phytochrome B-containing nuclear bodies (PNBs). Localisation to the PNBs is phosphorylation-dependent and is inhibited by the general Ser/Thr-kinase inhibitor Staurosporine. Upon ABA treatment, WRKY40 relocalises from PNBs to the nucleoplasm in an OST1-dependent manner. This stimulus-induced relocalisation was not observed in response to other abiotic or biotic stimuli, including NaCl, MeJA or flg22 treatment. Bimolecular fluorescence complementation experiments indicate that while PIF3, PIF4 and PHYB physically interact in these bodies, PHYB, PIF3 and PIF4 do not interact with the two WRKY transcription factors, which may suggest a more general role for these bodies in regulation of transcriptional activity. |
| doi_str_mv | 10.1038/srep13369 |
| format | Article |
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Arabidopsis thaliana
. Here we show that WRKY18 and WRKY40, but not WRKY60, co-localise with PIF3, PIF4 and PHYB to Phytochrome B-containing nuclear bodies (PNBs). Localisation to the PNBs is phosphorylation-dependent and is inhibited by the general Ser/Thr-kinase inhibitor Staurosporine. Upon ABA treatment, WRKY40 relocalises from PNBs to the nucleoplasm in an OST1-dependent manner. This stimulus-induced relocalisation was not observed in response to other abiotic or biotic stimuli, including NaCl, MeJA or flg22 treatment. Bimolecular fluorescence complementation experiments indicate that while PIF3, PIF4 and PHYB physically interact in these bodies, PHYB, PIF3 and PIF4 do not interact with the two WRKY transcription factors, which may suggest a more general role for these bodies in regulation of transcriptional activity.</description><identifier>ISSN: 2045-2322</identifier><identifier>EISSN: 2045-2322</identifier><identifier>DOI: 10.1038/srep13369</identifier><identifier>PMID: 26293691</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>14/19 ; 14/34 ; 631/449/448 ; 631/80/386/2382 ; Abscisic acid ; Abscisic Acid - pharmacology ; Arabidopsis - drug effects ; Arabidopsis - metabolism ; Arabidopsis Proteins - metabolism ; Cell Nucleus - drug effects ; Cell Nucleus - metabolism ; Complementation ; Enzyme inhibitors ; Fluorescence ; Gene regulation ; Humanities and Social Sciences ; multidisciplinary ; Nicotiana - metabolism ; Phosphorylation ; Phytochrome B ; Plant Leaves - metabolism ; Protein Transport - drug effects ; Protoplasts - drug effects ; Protoplasts - metabolism ; Science ; Signal transduction ; Sodium chloride ; Staurosporine ; Transcription factors ; Transcription Factors - metabolism ; Transduction</subject><ispartof>Scientific reports, 2015-08, Vol.5 (1), p.13369-13369, Article 13369</ispartof><rights>The Author(s) 2015</rights><rights>Copyright Nature Publishing Group Aug 2015</rights><rights>Copyright © 2015, Macmillan Publishers Limited 2015 Macmillan Publishers Limited</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c504t-6e4a2aaca0c5e600eb03eff00fa535702fd785b0b6fb3a5d1906b150b4295e0f3</citedby><cites>FETCH-LOGICAL-c504t-6e4a2aaca0c5e600eb03eff00fa535702fd785b0b6fb3a5d1906b150b4295e0f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4642543/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4642543/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,27903,27904,41099,42168,51554,53769,53771</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26293691$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Geilen, Katja</creatorcontrib><creatorcontrib>Böhmer, Maik</creatorcontrib><title>Dynamic subnuclear relocalisation of WRKY40 in response to Abscisic acid in Arabidopsis thaliana</title><title>Scientific reports</title><addtitle>Sci Rep</addtitle><addtitle>Sci Rep</addtitle><description>WRKY18, WRKY40 and WRKY60 are members of the WRKY transcription factor family and function as transcriptional regulators in ABA signal transduction in
Arabidopsis thaliana
. Here we show that WRKY18 and WRKY40, but not WRKY60, co-localise with PIF3, PIF4 and PHYB to Phytochrome B-containing nuclear bodies (PNBs). Localisation to the PNBs is phosphorylation-dependent and is inhibited by the general Ser/Thr-kinase inhibitor Staurosporine. Upon ABA treatment, WRKY40 relocalises from PNBs to the nucleoplasm in an OST1-dependent manner. This stimulus-induced relocalisation was not observed in response to other abiotic or biotic stimuli, including NaCl, MeJA or flg22 treatment. Bimolecular fluorescence complementation experiments indicate that while PIF3, PIF4 and PHYB physically interact in these bodies, PHYB, PIF3 and PIF4 do not interact with the two WRKY transcription factors, which may suggest a more general role for these bodies in regulation of transcriptional activity.</description><subject>14/19</subject><subject>14/34</subject><subject>631/449/448</subject><subject>631/80/386/2382</subject><subject>Abscisic acid</subject><subject>Abscisic Acid - pharmacology</subject><subject>Arabidopsis - drug effects</subject><subject>Arabidopsis - metabolism</subject><subject>Arabidopsis Proteins - metabolism</subject><subject>Cell Nucleus - drug effects</subject><subject>Cell Nucleus - metabolism</subject><subject>Complementation</subject><subject>Enzyme inhibitors</subject><subject>Fluorescence</subject><subject>Gene regulation</subject><subject>Humanities and Social Sciences</subject><subject>multidisciplinary</subject><subject>Nicotiana - metabolism</subject><subject>Phosphorylation</subject><subject>Phytochrome B</subject><subject>Plant Leaves - metabolism</subject><subject>Protein Transport - drug effects</subject><subject>Protoplasts - drug effects</subject><subject>Protoplasts - metabolism</subject><subject>Science</subject><subject>Signal transduction</subject><subject>Sodium chloride</subject><subject>Staurosporine</subject><subject>Transcription factors</subject><subject>Transcription Factors - metabolism</subject><subject>Transduction</subject><issn>2045-2322</issn><issn>2045-2322</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>C6C</sourceid><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNplkV1rFTEQhoMottRe-AdkwRsVjk4-9-RGONRPLAiiiFdxkk3alD3JNtkV-u9NOfVw1NxM4H3mnRleQh5TeEmBr1_V4ifKudL3yDEDIVeMM3b_4H9ETmu9gvYk04Lqh-SIKaZbBz0mP9_cJNxG19XFpsWNHktX_JgdjrHiHHPqcui-f_n0Q0AXU9PqlFP13Zy7ja0u1taLLg634qagjUOeaqzdfNkcMOEj8iDgWP3pXT0h3969_Xr2YXX--f3Hs835ykkQ80p5gQzRITjpFYC3wH0IAAEllz2wMPRracGqYDnKgWpQlkqwgmnpIfAT8nrnOy126wfn01xwNFOJWyw3JmM0fyspXpqL_MsIJZgUvBk8uzMo-XrxdTbbWJ0fR0w-L9XQHhSDXjPV0Kf_oFd5KamdZ-ha655L2utGPd9RruTaUgr7ZSiY2-jMPrrGPjncfk_-CaoBL3ZAbVK68OVg5H9uvwFzD6N4</recordid><startdate>20150821</startdate><enddate>20150821</enddate><creator>Geilen, Katja</creator><creator>Böhmer, Maik</creator><general>Nature Publishing Group UK</general><general>Nature Publishing Group</general><scope>C6C</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20150821</creationdate><title>Dynamic subnuclear relocalisation of WRKY40 in response to Abscisic acid in Arabidopsis thaliana</title><author>Geilen, Katja ; Böhmer, Maik</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c504t-6e4a2aaca0c5e600eb03eff00fa535702fd785b0b6fb3a5d1906b150b4295e0f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>14/19</topic><topic>14/34</topic><topic>631/449/448</topic><topic>631/80/386/2382</topic><topic>Abscisic acid</topic><topic>Abscisic Acid - pharmacology</topic><topic>Arabidopsis - drug effects</topic><topic>Arabidopsis - metabolism</topic><topic>Arabidopsis Proteins - metabolism</topic><topic>Cell Nucleus - drug effects</topic><topic>Cell Nucleus - metabolism</topic><topic>Complementation</topic><topic>Enzyme inhibitors</topic><topic>Fluorescence</topic><topic>Gene regulation</topic><topic>Humanities and Social Sciences</topic><topic>multidisciplinary</topic><topic>Nicotiana - metabolism</topic><topic>Phosphorylation</topic><topic>Phytochrome B</topic><topic>Plant Leaves - metabolism</topic><topic>Protein Transport - drug effects</topic><topic>Protoplasts - drug effects</topic><topic>Protoplasts - metabolism</topic><topic>Science</topic><topic>Signal transduction</topic><topic>Sodium chloride</topic><topic>Staurosporine</topic><topic>Transcription factors</topic><topic>Transcription Factors - metabolism</topic><topic>Transduction</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Geilen, Katja</creatorcontrib><creatorcontrib>Böhmer, Maik</creatorcontrib><collection>Springer Nature OA Free Journals</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health Medical collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection (Proquest) (PQ_SDU_P3)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Biological Sciences</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>ProQuest Science Journals</collection><collection>Biological Science Database</collection><collection>Publicly Available Content Database (Proquest) (PQ_SDU_P3)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Scientific reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Geilen, Katja</au><au>Böhmer, Maik</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Dynamic subnuclear relocalisation of WRKY40 in response to Abscisic acid in Arabidopsis thaliana</atitle><jtitle>Scientific reports</jtitle><stitle>Sci Rep</stitle><addtitle>Sci Rep</addtitle><date>2015-08-21</date><risdate>2015</risdate><volume>5</volume><issue>1</issue><spage>13369</spage><epage>13369</epage><pages>13369-13369</pages><artnum>13369</artnum><issn>2045-2322</issn><eissn>2045-2322</eissn><abstract>WRKY18, WRKY40 and WRKY60 are members of the WRKY transcription factor family and function as transcriptional regulators in ABA signal transduction in
Arabidopsis thaliana
. Here we show that WRKY18 and WRKY40, but not WRKY60, co-localise with PIF3, PIF4 and PHYB to Phytochrome B-containing nuclear bodies (PNBs). Localisation to the PNBs is phosphorylation-dependent and is inhibited by the general Ser/Thr-kinase inhibitor Staurosporine. Upon ABA treatment, WRKY40 relocalises from PNBs to the nucleoplasm in an OST1-dependent manner. This stimulus-induced relocalisation was not observed in response to other abiotic or biotic stimuli, including NaCl, MeJA or flg22 treatment. Bimolecular fluorescence complementation experiments indicate that while PIF3, PIF4 and PHYB physically interact in these bodies, PHYB, PIF3 and PIF4 do not interact with the two WRKY transcription factors, which may suggest a more general role for these bodies in regulation of transcriptional activity.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>26293691</pmid><doi>10.1038/srep13369</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | 14/19 14/34 631/449/448 631/80/386/2382 Abscisic acid Abscisic Acid - pharmacology Arabidopsis - drug effects Arabidopsis - metabolism Arabidopsis Proteins - metabolism Cell Nucleus - drug effects Cell Nucleus - metabolism Complementation Enzyme inhibitors Fluorescence Gene regulation Humanities and Social Sciences multidisciplinary Nicotiana - metabolism Phosphorylation Phytochrome B Plant Leaves - metabolism Protein Transport - drug effects Protoplasts - drug effects Protoplasts - metabolism Science Signal transduction Sodium chloride Staurosporine Transcription factors Transcription Factors - metabolism Transduction |
| title | Dynamic subnuclear relocalisation of WRKY40 in response to Abscisic acid in Arabidopsis thaliana |
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