Protein engineering of Cas9 for enhanced function

CRISPR/Cas systems act to protect the cell from invading nucleic acids in many bacteria and archaea. The bacterial immune protein Cas9 is a component of one of these CRISPR/Cas systems and has recently been adapted as a tool for genome editing. Cas9 is easily targeted to bind and cleave a DNA sequen...

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Veröffentlicht in:Methods in enzymology 2014, Vol.546, p.491-511
Hauptverfasser: Oakes, Benjamin L, Nadler, Dana C, Savage, David F
Format: Artikel
Sprache:eng
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