miR-132 can inhibit glioma cells invasion and migration by target MMP16 in vitro
Gliomas are the most common malignant primary brain tumors, and new clinical biomarkers and therapeutic targets are imminently required. MicroRNAs (miRNAs) are a novel class of small non-coding RNAs (∼22nt) involved in the regulation of various biological processes. Here, by using real-time polymera...
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| Veröffentlicht in: | OncoTargets and therapy 2015-01, Vol.8, p.3211-3218 |
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| creator | Wang, Hangzhou Li, Xue-Tao Wu, Chun Wu, Zhi-Wu Li, Yan-Yan Yang, Tian-Quan Chen, Gui-Lin Xie, Xue-Shun Huang, Yu-Lun Du, Zi-Wei Zhou, You-Xin |
| description | Gliomas are the most common malignant primary brain tumors, and new clinical biomarkers and therapeutic targets are imminently required. MicroRNAs (miRNAs) are a novel class of small non-coding RNAs (∼22nt) involved in the regulation of various biological processes. Here, by using real-time polymerase chain reaction, miRNA-132 was found to be significantly deregulated in glioma tissues. Based on the prediction of the target genes of miR-132, we hypothesized that there is a significant association between miR-132 and matrix metalloproteinase (MMP) 16 (MT3-MMP), a protein of the MMP family. We showed that the up-expression of miR-132 inhibited cell migration and invasion in the human glioma cell lines A172, SHG44, and U87. Furthermore, the overexpression of miR-132 reduced the expression of MMP16 in A172, SHG44, and U87 cells. Taken together, our study suggested that miR-132 affects glioma cell migration and invasion by MMP16 and implicates miR-132 as a metastasis-inhibiting miRNA in gliomas. |
| doi_str_mv | 10.2147/OTT.S79282 |
| format | Article |
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MicroRNAs (miRNAs) are a novel class of small non-coding RNAs (∼22nt) involved in the regulation of various biological processes. Here, by using real-time polymerase chain reaction, miRNA-132 was found to be significantly deregulated in glioma tissues. Based on the prediction of the target genes of miR-132, we hypothesized that there is a significant association between miR-132 and matrix metalloproteinase (MMP) 16 (MT3-MMP), a protein of the MMP family. We showed that the up-expression of miR-132 inhibited cell migration and invasion in the human glioma cell lines A172, SHG44, and U87. Furthermore, the overexpression of miR-132 reduced the expression of MMP16 in A172, SHG44, and U87 cells. Taken together, our study suggested that miR-132 affects glioma cell migration and invasion by MMP16 and implicates miR-132 as a metastasis-inhibiting miRNA in gliomas.</description><identifier>ISSN: 1178-6930</identifier><identifier>EISSN: 1178-6930</identifier><identifier>DOI: 10.2147/OTT.S79282</identifier><identifier>PMID: 26604788</identifier><language>eng</language><publisher>New Zealand: Dove Medical Press Limited</publisher><subject>Alzheimer's disease ; Bone cancer ; Brain cancer ; Brain research ; Cancer therapies ; Cell adhesion & migration ; Cell growth ; Development and progression ; Gastric cancer ; Gene expression ; Genetic aspects ; Genetic regulation ; Glioma ; Gliomas ; Health aspects ; Innovations ; Liver cancer ; Medical prognosis ; Melanoma ; Metastasis ; MicroRNA ; MicroRNAs ; Molecular targeted therapy ; Neurosurgery ; Original Research ; Properties ; Prostate cancer ; Proteases ; Proteins ; Tumors</subject><ispartof>OncoTargets and therapy, 2015-01, Vol.8, p.3211-3218</ispartof><rights>COPYRIGHT 2015 Dove Medical Press Limited</rights><rights>2015. This work is licensed under https://creativecommons.org/licenses/by-nc/3.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2015 Wang et al. This work is published by Dove Medical Press Limited, and licensed under Creative Commons Attribution – Non Commercial (unported, v3.0) License 2015</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c504t-6172c8a72d39aff6d162bdce49db922d6ebb57afb89ccdde9f77321fba99f67b3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4640442/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4640442/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,3862,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26604788$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wang, Hangzhou</creatorcontrib><creatorcontrib>Li, Xue-Tao</creatorcontrib><creatorcontrib>Wu, Chun</creatorcontrib><creatorcontrib>Wu, Zhi-Wu</creatorcontrib><creatorcontrib>Li, Yan-Yan</creatorcontrib><creatorcontrib>Yang, Tian-Quan</creatorcontrib><creatorcontrib>Chen, Gui-Lin</creatorcontrib><creatorcontrib>Xie, Xue-Shun</creatorcontrib><creatorcontrib>Huang, Yu-Lun</creatorcontrib><creatorcontrib>Du, Zi-Wei</creatorcontrib><creatorcontrib>Zhou, You-Xin</creatorcontrib><title>miR-132 can inhibit glioma cells invasion and migration by target MMP16 in vitro</title><title>OncoTargets and therapy</title><addtitle>Onco Targets Ther</addtitle><description>Gliomas are the most common malignant primary brain tumors, and new clinical biomarkers and therapeutic targets are imminently required. MicroRNAs (miRNAs) are a novel class of small non-coding RNAs (∼22nt) involved in the regulation of various biological processes. Here, by using real-time polymerase chain reaction, miRNA-132 was found to be significantly deregulated in glioma tissues. Based on the prediction of the target genes of miR-132, we hypothesized that there is a significant association between miR-132 and matrix metalloproteinase (MMP) 16 (MT3-MMP), a protein of the MMP family. We showed that the up-expression of miR-132 inhibited cell migration and invasion in the human glioma cell lines A172, SHG44, and U87. Furthermore, the overexpression of miR-132 reduced the expression of MMP16 in A172, SHG44, and U87 cells. Taken together, our study suggested that miR-132 affects glioma cell migration and invasion by MMP16 and implicates miR-132 as a metastasis-inhibiting miRNA in gliomas.</description><subject>Alzheimer's disease</subject><subject>Bone cancer</subject><subject>Brain cancer</subject><subject>Brain research</subject><subject>Cancer therapies</subject><subject>Cell adhesion & migration</subject><subject>Cell growth</subject><subject>Development and progression</subject><subject>Gastric cancer</subject><subject>Gene expression</subject><subject>Genetic aspects</subject><subject>Genetic regulation</subject><subject>Glioma</subject><subject>Gliomas</subject><subject>Health aspects</subject><subject>Innovations</subject><subject>Liver cancer</subject><subject>Medical prognosis</subject><subject>Melanoma</subject><subject>Metastasis</subject><subject>MicroRNA</subject><subject>MicroRNAs</subject><subject>Molecular targeted therapy</subject><subject>Neurosurgery</subject><subject>Original Research</subject><subject>Properties</subject><subject>Prostate cancer</subject><subject>Proteases</subject><subject>Proteins</subject><subject>Tumors</subject><issn>1178-6930</issn><issn>1178-6930</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>8G5</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNptkl1rHCEUhofS0Hy0N_0BRSiUUJjt6Lg63hRC6EcgIaHdXoufs4YZTdRZyL-vw6bJbihe6Dk-55VzfKvqPWwWCGL65Xq1WvymDHXoVXUEIe1qwtrm9c75sDpO6bZpCOkQflMdIkIaTLvuqLoZ3a8atggo4YHzayddBv3gwiiAMsOQSnIjkgseCK_B6Poo8hzJB5BF7E0GV1c3kBQMbFyO4W11YMWQzLvH_aT68_3b6vxnfXn94-L87LJWywbnmkCKVCco0i0T1hINCZJaGcy0ZAhpYqRcUmFlx5TS2jBLaYuglYIxS6hsT6qvW927SY6mVPocxcDvohtFfOBBOL5_492a92HDMcENxqgInD4KxHA_mZT56NLcsvAmTIlDihlpWtbBgn58gd6GKfrSHkcIo2WZM2XPVC8Gw523obyrZlF-hnEBIOpmavEfqixtRqeCN9aV_F7Bp52CtRFDXqcwTPMvpH3w8xZUMaQUjX0aBmz4bBRejMK3Rinwh93xPaH_nNH-BX73toU</recordid><startdate>20150101</startdate><enddate>20150101</enddate><creator>Wang, Hangzhou</creator><creator>Li, Xue-Tao</creator><creator>Wu, Chun</creator><creator>Wu, Zhi-Wu</creator><creator>Li, Yan-Yan</creator><creator>Yang, Tian-Quan</creator><creator>Chen, Gui-Lin</creator><creator>Xie, Xue-Shun</creator><creator>Huang, Yu-Lun</creator><creator>Du, Zi-Wei</creator><creator>Zhou, You-Xin</creator><general>Dove Medical Press Limited</general><general>Taylor & Francis Ltd</general><general>Dove Medical Press</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M2O</scope><scope>M7P</scope><scope>MBDVC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20150101</creationdate><title>miR-132 can inhibit glioma cells invasion and migration by target MMP16 in vitro</title><author>Wang, Hangzhou ; Li, Xue-Tao ; Wu, Chun ; Wu, Zhi-Wu ; Li, Yan-Yan ; Yang, Tian-Quan ; Chen, Gui-Lin ; Xie, Xue-Shun ; Huang, Yu-Lun ; Du, Zi-Wei ; Zhou, You-Xin</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c504t-6172c8a72d39aff6d162bdce49db922d6ebb57afb89ccdde9f77321fba99f67b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Alzheimer's disease</topic><topic>Bone cancer</topic><topic>Brain cancer</topic><topic>Brain research</topic><topic>Cancer therapies</topic><topic>Cell adhesion & migration</topic><topic>Cell growth</topic><topic>Development and progression</topic><topic>Gastric cancer</topic><topic>Gene expression</topic><topic>Genetic aspects</topic><topic>Genetic regulation</topic><topic>Glioma</topic><topic>Gliomas</topic><topic>Health aspects</topic><topic>Innovations</topic><topic>Liver cancer</topic><topic>Medical prognosis</topic><topic>Melanoma</topic><topic>Metastasis</topic><topic>MicroRNA</topic><topic>MicroRNAs</topic><topic>Molecular targeted therapy</topic><topic>Neurosurgery</topic><topic>Original Research</topic><topic>Properties</topic><topic>Prostate cancer</topic><topic>Proteases</topic><topic>Proteins</topic><topic>Tumors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wang, Hangzhou</creatorcontrib><creatorcontrib>Li, Xue-Tao</creatorcontrib><creatorcontrib>Wu, Chun</creatorcontrib><creatorcontrib>Wu, Zhi-Wu</creatorcontrib><creatorcontrib>Li, Yan-Yan</creatorcontrib><creatorcontrib>Yang, Tian-Quan</creatorcontrib><creatorcontrib>Chen, Gui-Lin</creatorcontrib><creatorcontrib>Xie, Xue-Shun</creatorcontrib><creatorcontrib>Huang, Yu-Lun</creatorcontrib><creatorcontrib>Du, Zi-Wei</creatorcontrib><creatorcontrib>Zhou, You-Xin</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Research Library</collection><collection>Biological Science Database</collection><collection>Research Library (Corporate)</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>OncoTargets and therapy</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wang, Hangzhou</au><au>Li, Xue-Tao</au><au>Wu, Chun</au><au>Wu, Zhi-Wu</au><au>Li, Yan-Yan</au><au>Yang, Tian-Quan</au><au>Chen, Gui-Lin</au><au>Xie, Xue-Shun</au><au>Huang, Yu-Lun</au><au>Du, Zi-Wei</au><au>Zhou, You-Xin</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>miR-132 can inhibit glioma cells invasion and migration by target MMP16 in vitro</atitle><jtitle>OncoTargets and therapy</jtitle><addtitle>Onco Targets Ther</addtitle><date>2015-01-01</date><risdate>2015</risdate><volume>8</volume><spage>3211</spage><epage>3218</epage><pages>3211-3218</pages><issn>1178-6930</issn><eissn>1178-6930</eissn><abstract>Gliomas are the most common malignant primary brain tumors, and new clinical biomarkers and therapeutic targets are imminently required. MicroRNAs (miRNAs) are a novel class of small non-coding RNAs (∼22nt) involved in the regulation of various biological processes. Here, by using real-time polymerase chain reaction, miRNA-132 was found to be significantly deregulated in glioma tissues. Based on the prediction of the target genes of miR-132, we hypothesized that there is a significant association between miR-132 and matrix metalloproteinase (MMP) 16 (MT3-MMP), a protein of the MMP family. We showed that the up-expression of miR-132 inhibited cell migration and invasion in the human glioma cell lines A172, SHG44, and U87. Furthermore, the overexpression of miR-132 reduced the expression of MMP16 in A172, SHG44, and U87 cells. Taken together, our study suggested that miR-132 affects glioma cell migration and invasion by MMP16 and implicates miR-132 as a metastasis-inhibiting miRNA in gliomas.</abstract><cop>New Zealand</cop><pub>Dove Medical Press Limited</pub><pmid>26604788</pmid><doi>10.2147/OTT.S79282</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Alzheimer's disease Bone cancer Brain cancer Brain research Cancer therapies Cell adhesion & migration Cell growth Development and progression Gastric cancer Gene expression Genetic aspects Genetic regulation Glioma Gliomas Health aspects Innovations Liver cancer Medical prognosis Melanoma Metastasis MicroRNA MicroRNAs Molecular targeted therapy Neurosurgery Original Research Properties Prostate cancer Proteases Proteins Tumors |
| title | miR-132 can inhibit glioma cells invasion and migration by target MMP16 in vitro |
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