Characterization of mesenchymal stem cells derived from the equine synovial fluid and membrane
Isolation of mesenchymal stem cells (MSCs) in equines, has been reported for different tissues including bone marrow, adipose, umbilical cord, peripheral blood, and yolk sac. In regard to the MSCs derived from synovial fluid (SF) or membrane (SM), there is data available for humans, dogs, pigs, goat...
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description | Isolation of mesenchymal stem cells (MSCs) in equines, has been reported for different tissues including bone marrow, adipose, umbilical cord, peripheral blood, and yolk sac. In regard to the MSCs derived from synovial fluid (SF) or membrane (SM), there is data available for humans, dogs, pigs, goats and horses. Especially in equines, these cells have being considered promising candidates for articular regeneration. Herein, we established and characterized MSCs obtained from equine SF and SM. Samples were obtained during arthroscopy and cultured using MEM (Minimum Essential Medium). MSCs were characterized by morphology and expression of specific markers for stem cells, pluripotency, inflammation, and cell cycle.
The medium MEM was more effective (97% ± 2) to maintain both cultures. The cultures were composed by adherent cells with fibroblast-like shape, which had a growth pattern represented by a sigmoidal curve. After the expansion, the cells were analyzed by flow cytometry for stem cells, inflammatory, and cell cycle markers, and both lineages showed significant expression of CD45, Oct3/4, Nanog, CD105, CD90, CD34, CD117, CD133, TRA-1-81, VEGF, and LY6a. In contrast, there were differences in the cell cycle phases between the lineages, which was not observed in relation to the mitochondrial electrical potential.
Given the large impact that joint pathology has on the athletic performance horses, our results suggested that the SF and SM are promising sources of stem cells with satisfactory characteristics of growth and gene expression that can be used in equine regenerative medicine. |
doi_str_mv | 10.1186/s12917-015-0531-5 |
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The medium MEM was more effective (97% ± 2) to maintain both cultures. The cultures were composed by adherent cells with fibroblast-like shape, which had a growth pattern represented by a sigmoidal curve. After the expansion, the cells were analyzed by flow cytometry for stem cells, inflammatory, and cell cycle markers, and both lineages showed significant expression of CD45, Oct3/4, Nanog, CD105, CD90, CD34, CD117, CD133, TRA-1-81, VEGF, and LY6a. In contrast, there were differences in the cell cycle phases between the lineages, which was not observed in relation to the mitochondrial electrical potential.
Given the large impact that joint pathology has on the athletic performance horses, our results suggested that the SF and SM are promising sources of stem cells with satisfactory characteristics of growth and gene expression that can be used in equine regenerative medicine.</description><identifier>ISSN: 1746-6148</identifier><identifier>EISSN: 1746-6148</identifier><identifier>DOI: 10.1186/s12917-015-0531-5</identifier><identifier>PMID: 26555093</identifier><language>eng</language><publisher>England: BioMed Central Ltd</publisher><subject>Analysis ; Animals ; Cell Cycle ; Cell Proliferation ; Cells, Cultured ; Horses ; Membrane Potential, Mitochondrial ; Mesenchymal Stromal Cells - cytology ; Mesenchymal Stromal Cells - physiology ; Regenerative medicine ; Stem cells ; Synovial fluid ; Synovial Fluid - cytology ; Synovial Membrane - cytology ; Vascular endothelial growth factor</subject><ispartof>BMC veterinary research, 2015-11, Vol.11 (281), p.281-281, Article 281</ispartof><rights>COPYRIGHT 2015 BioMed Central Ltd.</rights><rights>Copyright BioMed Central 2015</rights><rights>Prado et al. 2015</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c560t-7da5cca82175f0189f4cad3a64099d0613d1d60d96d34b111b76eaffc8a188bb3</citedby><cites>FETCH-LOGICAL-c560t-7da5cca82175f0189f4cad3a64099d0613d1d60d96d34b111b76eaffc8a188bb3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4640348/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4640348/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26555093$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Prado, Aline Ambrogi Franco</creatorcontrib><creatorcontrib>Favaron, Phelipe Oliveira</creatorcontrib><creatorcontrib>da Silva, Luis Claudio Lopes Correia</creatorcontrib><creatorcontrib>Baccarin, Raquel Yvonne Arantes</creatorcontrib><creatorcontrib>Miglino, Maria Angelica</creatorcontrib><creatorcontrib>Maria, Durvanei Augusto</creatorcontrib><title>Characterization of mesenchymal stem cells derived from the equine synovial fluid and membrane</title><title>BMC veterinary research</title><addtitle>BMC Vet Res</addtitle><description>Isolation of mesenchymal stem cells (MSCs) in equines, has been reported for different tissues including bone marrow, adipose, umbilical cord, peripheral blood, and yolk sac. In regard to the MSCs derived from synovial fluid (SF) or membrane (SM), there is data available for humans, dogs, pigs, goats and horses. Especially in equines, these cells have being considered promising candidates for articular regeneration. Herein, we established and characterized MSCs obtained from equine SF and SM. Samples were obtained during arthroscopy and cultured using MEM (Minimum Essential Medium). MSCs were characterized by morphology and expression of specific markers for stem cells, pluripotency, inflammation, and cell cycle.
The medium MEM was more effective (97% ± 2) to maintain both cultures. The cultures were composed by adherent cells with fibroblast-like shape, which had a growth pattern represented by a sigmoidal curve. After the expansion, the cells were analyzed by flow cytometry for stem cells, inflammatory, and cell cycle markers, and both lineages showed significant expression of CD45, Oct3/4, Nanog, CD105, CD90, CD34, CD117, CD133, TRA-1-81, VEGF, and LY6a. In contrast, there were differences in the cell cycle phases between the lineages, which was not observed in relation to the mitochondrial electrical potential.
Given the large impact that joint pathology has on the athletic performance horses, our results suggested that the SF and SM are promising sources of stem cells with satisfactory characteristics of growth and gene expression that can be used in equine regenerative medicine.</description><subject>Analysis</subject><subject>Animals</subject><subject>Cell Cycle</subject><subject>Cell Proliferation</subject><subject>Cells, Cultured</subject><subject>Horses</subject><subject>Membrane Potential, Mitochondrial</subject><subject>Mesenchymal Stromal Cells - cytology</subject><subject>Mesenchymal Stromal Cells - physiology</subject><subject>Regenerative medicine</subject><subject>Stem cells</subject><subject>Synovial fluid</subject><subject>Synovial Fluid - cytology</subject><subject>Synovial Membrane - cytology</subject><subject>Vascular endothelial growth factor</subject><issn>1746-6148</issn><issn>1746-6148</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><recordid>eNptkk1rFTEYhQdRbK3-ADcScONmat6bj0k2Qrn4BQU3ujVk8tGbMknaZObC9deb4dbaimSRkDznvJxwuu414HMAwd9X2EgYegysx4xAz550pzBQ3nOg4umD80n3otZrjCmVA3_enWw4YwxLctr93O500WZ2JfzSc8gJZY-iqy6Z3SHqCdXZRWTcNFVkG7R3FvmSI5p3DrnbJSSH6iHlfWisn5ZgkU62OcSx6OReds-8nqp7dbefdT8-ffy-_dJffvv8dXtx2RvG8dwPVjNjtNjAwDwGIT012hLNKZbSYg7EguXYSm4JHQFgHLjT3huhQYhxJGfdh6PvzTJGZ41Lc9GTuikh6nJQWQf1-CWFnbrKe0XbCEJFM3h3Z1Dy7eLqrGKoa-wWIi9VwUAISMAEN_TtP-h1Xkpq8Ro1SMFBwOYvdaUnp0Lyuc01q6m6oFRgJhlhjTr_D9WWdTGYnJwP7f6RAI4CU3Ktxfn7jIDVWgp1LIVqpVBrKdSqefPwc-4Vf1pAfgMasbKa</recordid><startdate>20151110</startdate><enddate>20151110</enddate><creator>Prado, Aline Ambrogi Franco</creator><creator>Favaron, Phelipe Oliveira</creator><creator>da Silva, Luis Claudio Lopes Correia</creator><creator>Baccarin, Raquel Yvonne Arantes</creator><creator>Miglino, Maria Angelica</creator><creator>Maria, Durvanei Augusto</creator><general>BioMed Central Ltd</general><general>BioMed Central</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QG</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>H94</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20151110</creationdate><title>Characterization of mesenchymal stem cells derived from the equine synovial fluid and membrane</title><author>Prado, Aline Ambrogi Franco ; Favaron, Phelipe Oliveira ; da Silva, Luis Claudio Lopes Correia ; Baccarin, Raquel Yvonne Arantes ; Miglino, Maria Angelica ; Maria, Durvanei Augusto</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c560t-7da5cca82175f0189f4cad3a64099d0613d1d60d96d34b111b76eaffc8a188bb3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Analysis</topic><topic>Animals</topic><topic>Cell Cycle</topic><topic>Cell Proliferation</topic><topic>Cells, Cultured</topic><topic>Horses</topic><topic>Membrane Potential, Mitochondrial</topic><topic>Mesenchymal Stromal Cells - cytology</topic><topic>Mesenchymal Stromal Cells - physiology</topic><topic>Regenerative medicine</topic><topic>Stem cells</topic><topic>Synovial fluid</topic><topic>Synovial Fluid - cytology</topic><topic>Synovial Membrane - cytology</topic><topic>Vascular endothelial growth factor</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Prado, Aline Ambrogi Franco</creatorcontrib><creatorcontrib>Favaron, Phelipe Oliveira</creatorcontrib><creatorcontrib>da Silva, Luis Claudio Lopes Correia</creatorcontrib><creatorcontrib>Baccarin, Raquel Yvonne Arantes</creatorcontrib><creatorcontrib>Miglino, Maria Angelica</creatorcontrib><creatorcontrib>Maria, Durvanei Augusto</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Access via ProQuest (Open Access)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>BMC veterinary research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Prado, Aline Ambrogi Franco</au><au>Favaron, Phelipe Oliveira</au><au>da Silva, Luis Claudio Lopes Correia</au><au>Baccarin, Raquel Yvonne Arantes</au><au>Miglino, Maria Angelica</au><au>Maria, Durvanei Augusto</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Characterization of mesenchymal stem cells derived from the equine synovial fluid and membrane</atitle><jtitle>BMC veterinary research</jtitle><addtitle>BMC Vet Res</addtitle><date>2015-11-10</date><risdate>2015</risdate><volume>11</volume><issue>281</issue><spage>281</spage><epage>281</epage><pages>281-281</pages><artnum>281</artnum><issn>1746-6148</issn><eissn>1746-6148</eissn><abstract>Isolation of mesenchymal stem cells (MSCs) in equines, has been reported for different tissues including bone marrow, adipose, umbilical cord, peripheral blood, and yolk sac. In regard to the MSCs derived from synovial fluid (SF) or membrane (SM), there is data available for humans, dogs, pigs, goats and horses. Especially in equines, these cells have being considered promising candidates for articular regeneration. Herein, we established and characterized MSCs obtained from equine SF and SM. Samples were obtained during arthroscopy and cultured using MEM (Minimum Essential Medium). MSCs were characterized by morphology and expression of specific markers for stem cells, pluripotency, inflammation, and cell cycle.
The medium MEM was more effective (97% ± 2) to maintain both cultures. The cultures were composed by adherent cells with fibroblast-like shape, which had a growth pattern represented by a sigmoidal curve. After the expansion, the cells were analyzed by flow cytometry for stem cells, inflammatory, and cell cycle markers, and both lineages showed significant expression of CD45, Oct3/4, Nanog, CD105, CD90, CD34, CD117, CD133, TRA-1-81, VEGF, and LY6a. In contrast, there were differences in the cell cycle phases between the lineages, which was not observed in relation to the mitochondrial electrical potential.
Given the large impact that joint pathology has on the athletic performance horses, our results suggested that the SF and SM are promising sources of stem cells with satisfactory characteristics of growth and gene expression that can be used in equine regenerative medicine.</abstract><cop>England</cop><pub>BioMed Central Ltd</pub><pmid>26555093</pmid><doi>10.1186/s12917-015-0531-5</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record> |
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subjects | Analysis Animals Cell Cycle Cell Proliferation Cells, Cultured Horses Membrane Potential, Mitochondrial Mesenchymal Stromal Cells - cytology Mesenchymal Stromal Cells - physiology Regenerative medicine Stem cells Synovial fluid Synovial Fluid - cytology Synovial Membrane - cytology Vascular endothelial growth factor |
title | Characterization of mesenchymal stem cells derived from the equine synovial fluid and membrane |
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