Recovery of the human immunodeficiency virus from fibreoptic bronchoscopes
Ten bronchoscopes that had been used on patients with the acquired immunodeficiency syndrome were sampled to determine the nature and extent of microbial contamination. Samples were taken by irrigating the suction biopsy channel with modified viral transport medium and by swabbing the insertion tube...
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| Veröffentlicht in: | Thorax 1991-06, Vol.46 (6), p.410-412 |
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| creator | Hanson, P J Gor, D Clarke, J R Chadwick, M V Gazzard, B Jeffries, D J Gaya, H Collins, J V |
| description | Ten bronchoscopes that had been used on patients with the acquired immunodeficiency syndrome were sampled to determine the nature and extent of microbial contamination. Samples were taken by irrigating the suction biopsy channel with modified viral transport medium and by swabbing the insertion tube. Sampling was repeated after they had been cleaned in detergent and after two minutes' disinfection in 2% alkaline glutaraldehyde. Before being cleaned the seven bronchoscopes tested by polymerase chain reaction were contaminated with the human immunodeficiency virus, though infectivity and antigen assays gave negative results. Other organisms identified were hepatitis B virus (1), commensal bacteria (9), and Pneumocystis carinii (4). Mean bacterial contamination was 2.27 log colony forming organisms per millilitre. Cleaning the bronchoscope before disinfection removed all detectable contaminants with a reduction in bacterial growth of up to 8 log colony forming units/ml. |
| doi_str_mv | 10.1136/thx.46.6.410 |
| format | Article |
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Samples were taken by irrigating the suction biopsy channel with modified viral transport medium and by swabbing the insertion tube. Sampling was repeated after they had been cleaned in detergent and after two minutes' disinfection in 2% alkaline glutaraldehyde. Before being cleaned the seven bronchoscopes tested by polymerase chain reaction were contaminated with the human immunodeficiency virus, though infectivity and antigen assays gave negative results. Other organisms identified were hepatitis B virus (1), commensal bacteria (9), and Pneumocystis carinii (4). Mean bacterial contamination was 2.27 log colony forming organisms per millilitre. 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Immunoglobulinopathies ; Immunopathology ; Medical sciences</subject><ispartof>Thorax, 1991-06, Vol.46 (6), p.410-412</ispartof><rights>1991 INIST-CNRS</rights><rights>Copyright BMJ Publishing Group LTD Jun 1991</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-b4870-12af107cc3425708b8f06750a38b0ccea3be5fe85184cad278a56286a96695a23</citedby><cites>FETCH-LOGICAL-b4870-12af107cc3425708b8f06750a38b0ccea3be5fe85184cad278a56286a96695a23</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC463186/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC463186/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=19830030$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1858078$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hanson, P J</creatorcontrib><creatorcontrib>Gor, D</creatorcontrib><creatorcontrib>Clarke, J R</creatorcontrib><creatorcontrib>Chadwick, M V</creatorcontrib><creatorcontrib>Gazzard, B</creatorcontrib><creatorcontrib>Jeffries, D J</creatorcontrib><creatorcontrib>Gaya, H</creatorcontrib><creatorcontrib>Collins, J V</creatorcontrib><title>Recovery of the human immunodeficiency virus from fibreoptic bronchoscopes</title><title>Thorax</title><addtitle>Thorax</addtitle><description>Ten bronchoscopes that had been used on patients with the acquired immunodeficiency syndrome were sampled to determine the nature and extent of microbial contamination. Samples were taken by irrigating the suction biopsy channel with modified viral transport medium and by swabbing the insertion tube. Sampling was repeated after they had been cleaned in detergent and after two minutes' disinfection in 2% alkaline glutaraldehyde. Before being cleaned the seven bronchoscopes tested by polymerase chain reaction were contaminated with the human immunodeficiency virus, though infectivity and antigen assays gave negative results. Other organisms identified were hepatitis B virus (1), commensal bacteria (9), and Pneumocystis carinii (4). Mean bacterial contamination was 2.27 log colony forming organisms per millilitre. 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Immunoglobulinopathies</topic><topic>Immunopathology</topic><topic>Medical sciences</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hanson, P J</creatorcontrib><creatorcontrib>Gor, D</creatorcontrib><creatorcontrib>Clarke, J R</creatorcontrib><creatorcontrib>Chadwick, M V</creatorcontrib><creatorcontrib>Gazzard, B</creatorcontrib><creatorcontrib>Jeffries, D J</creatorcontrib><creatorcontrib>Gaya, H</creatorcontrib><creatorcontrib>Collins, J V</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central</collection><collection>BMJ Journals</collection><collection>ProQuest One Community College</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Thorax</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hanson, P J</au><au>Gor, D</au><au>Clarke, J R</au><au>Chadwick, M V</au><au>Gazzard, B</au><au>Jeffries, D J</au><au>Gaya, H</au><au>Collins, J V</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Recovery of the human immunodeficiency virus from fibreoptic bronchoscopes</atitle><jtitle>Thorax</jtitle><addtitle>Thorax</addtitle><date>1991-06-01</date><risdate>1991</risdate><volume>46</volume><issue>6</issue><spage>410</spage><epage>412</epage><pages>410-412</pages><issn>0040-6376</issn><eissn>1468-3296</eissn><coden>THORA7</coden><abstract>Ten bronchoscopes that had been used on patients with the acquired immunodeficiency syndrome were sampled to determine the nature and extent of microbial contamination. 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| ispartof | Thorax, 1991-06, Vol.46 (6), p.410-412 |
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| language | eng |
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| source | MEDLINE; EZB-FREE-00999 freely available EZB journals; PubMed Central; Alma/SFX Local Collection |
| subjects | AIDS/HIV Biological and medical sciences Bronchoscopes DNA, Viral - analysis Enzyme-Linked Immunosorbent Assay Equipment Contamination Fiber Optic Technology HIV - isolation & purification Humans Immunodeficiencies Immunodeficiencies. Immunoglobulinopathies Immunopathology Medical sciences |
| title | Recovery of the human immunodeficiency virus from fibreoptic bronchoscopes |
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